Filtered By:
Education: Academia

This page shows you your search results in order of date. This is page number 2.

Order by Relevance | Date

Total 93 results found since Jan 2013.

Abstracts of Presentations at the Association of Clinical Scientists 143 < sup > rd < /sup > Meeting Louisville, KY May 11-14,2022
Conclusion: These assays are suitable for routine diagnostic. The UltraFast NextGenPCR is the fastest with average time (30mins), followed by Agilent (2 hrs) and MassArray (6hrs). Upon completion of this activity, participants should be able to examine, measure and compare results from different assays for SARS detection, evaluate and diagnose accurately, as well as being able to plan, organize and recommend a diagnostic procedure for diagnostic laboratory. Key words: SARS-CoV-2, RNA extraction, RT-PCR, limit of detection, quantification cycle, COVID-19, in vitro diagnostic tests, Agilent, Massarray, Ultrafast. [20] From t...
Source: Annals of Clinical and Laboratory Science - July 1, 2022 Category: Laboratory Medicine Source Type: research

Multifunctional Applications of Engineered Extracellular Vesicles in the Treatment of Cancer.
Abstract Extracellular vesicles (EVs) are key players of intercellular communication in physiological and pathological conditions. In cancer, EVs mediate complex signaling mechanisms between cancer cells and the tumor microenvironment, and can influence tumor progression and the response to existing therapies. Importantly, EVs can be loaded with therapeutic agents and modified to display tumor-targeting molecules. In the field of nanomedicine, EVs have been engineered to serve as therapeutic delivery vehicles for several anti-cancer agents, including antibodies, chemotherapy, compounds, CRISPR/Cas9 and siRNA. Nota...
Source: Endocrinology - January 7, 2021 Category: Endocrinology Authors: Kugeratski FG, McAndrews KM, Kalluri R Tags: Endocrinology Source Type: research

Characterization of Positively Charged Polyplexes by Tunable Resistive Pulse Sensing.
This article describes a new coating solution based on choline-chloride. Coating is verified by current-voltage (I-V) recordings and ultimately tested on a positively charged nanoparticle formulation comprising of siRNA and PEG-PCL-PEI polymer. This coating allows successful size, polydispersity index (PDI) and concentration measurement by tunable resistive pulse sensing of positively charged PEI-based polyplexes. This article provides the foundation for further characterization of polyplexes as well as other positively charged nanoparticle formulations based on particle by particle measurements. PMID: 33338601 [PubMe...
Source: European Journal of Pharmaceutics and Biopharmaceutics - December 15, 2020 Category: Drugs & Pharmacology Authors: Wm Keil T, Merkel OM Tags: Eur J Pharm Biopharm Source Type: research

The SARS-CoV-2 receptor, Angiotensin converting enzyme 2 (ACE2) is required for human endometrial stromal cell decidualization.
Abstract The coronavirus disease 2019 (COVID-19) first appeared in December 2019 and rapidly spread throughout the world. The SARS-CoV-2 virus enters the host cells by binding to the angiotensin-converting enzyme (ACE2). Although much of the focus is on respiratory symptoms, recent reports suggest that SARS-CoV-2 can cause pregnancy complications such as pre-term birth and miscarriages; and women with COVID-19 had maternal vascular malperfusion and decidual arteriopathy in their placentas. Here, we report that ACE2 protein is expressed in both endometrial epithelial and stromal cells in the proliferative phase of ...
Source: Biology of Reproduction - November 18, 2020 Category: Reproduction Medicine Authors: Chadchan SB, Popli P, Maurya VK, Kommagani R Tags: Biol Reprod Source Type: research

Sirtuin-1 Inhibits Endothelin-2 Expression in Human Granulosa-Lutein Cells via HIF1A and Epigenetic Modifications †.
Sirtuin-1 Inhibits Endothelin-2 Expression in Human Granulosa-Lutein Cells via HIF1A and Epigenetic Modifications†. Biol Reprod. 2020 Oct 28;: Authors: Szymanska M, Manthe S, Shrestha K, Girsh E, Harlev A, Kisliouk T, Meidan R Abstract Endothelin-2 (EDN2) expression in granulosa cells was previously shown to be highly dependent on the hypoxic mediator, HIF1A. Here we investigated whether sirtuin-1 (SIRT1), by deacetylating HIF1A and class III histones, modulates EDN2 in human granulosa-lutein cells (hGLCs). We found that HIF1A was markedly suppressed in the presence of resveratrol or a specific SIRT...
Source: Biology of Reproduction - October 28, 2020 Category: Reproduction Medicine Authors: Szymanska M, Manthe S, Shrestha K, Girsh E, Harlev A, Kisliouk T, Meidan R Tags: Biol Reprod Source Type: research

Advancement of PI3 Kinase Inhibitor Combination Therapies for PI3K-Aberrant Chordoma
Conclusion High throughput with in vitro combination screening is feasible with chordoma cells and allows for rapid identification of synergistic dual-therapies. Potential combination therapies and targetable pathways were identified. T (brachyury) knockdown produced significant reduction in cell viability, but did not show additional benefit with PI3K pathway inhibition in this model. Further in vitro and in vivo validation of these therapeutic combinations is warranted. [...] Georg Thieme Verlag KG Stuttgart · New YorkArticle in Thieme eJournals: Table of contents  |  Abstract  |  Full text
Source: Journal of Neurological Surgery Part B: Skull Base - October 12, 2020 Category: Neurosurgery Authors: Heft Neal, Molly E. Michmerhuizen, Nicole L. Kovatch, Kevin J. Owen, John Henry J. Zhai, Jingyi Jiang, Hui McKean, Erin L. Prince, Mark E.P. Brenner, J. Chad Tags: Original Article Source Type: research

Nrf2/ARE pathway activation is involved in negatively regulating heat-induced apoptosis in non-small cell lung cancer cells.
In this study, using immunocytochemistry and western blot analysis, we demonstrated that heat stress induced Nrf2/ARE activation through the nuclear translocation of Nrf2 in non-small cell lung cancer cells. Luciferase activity was also increased. Additionally, antioxidant enzymes were increased through Nrf2 activation after heat stress. Transfection of lung cancer cells with siRNA directed against Nrf2 increased heat cytotoxicity and cell apoptosis. Heat stress could induce reactive oxygen species (ROS) accumulation, while the antioxidant NAC obviously reduced cell apoptosis ratio, indicating that heat stress induced cell...
Source: Acta Biochimica et Biophysica Sinica - April 6, 2020 Category: Biochemistry Authors: Xie W, Tan B, Yang Z, Yu X, Chen L, Ran D, Xu Q, Zhou X Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

FoxM1 affects adhesive, migratory, and invasive abilities of human retinoblastoma Y-79 cells by targeting matrix metalloproteinase 2.
In this study, we aimed to investigate the correlation between FoxM1 and retinoblastoma (Rb) metastasis and to explore the detailed mechanism. Wound healing, cell adhesion, and invasion assays showed that FoxM1 overexpression induced epithelial-mesenchymal transition in Y-79 cells and inhibited adhesion and subsequently promoted metastasis of Y-79 cells, while FoxM1 knockdown showed the opposite effects. A luciferase reporter assay and chromatin immunoprecipitation assay provided evidence that FoxM1 promoted matrix metalloproteinase 2 (MMP2) transcription by directly binding to and promoting MMP2 promoter. MMP2 knockdown b...
Source: Acta Biochimica et Biophysica Sinica - March 9, 2020 Category: Biochemistry Authors: Zhu X, Yu M, Wang K, Zou W, Zhu L Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Interleukin-6 (IL-6) activates the NOTCH1 signaling pathway through E-proteins in endometriotic lesions.
CONCLUSIONS: IL-6 induced NOTCH1 expression is mediated by E-proteins in the ectopic GE cells, which may promote endometriotic lesion development. PMID: 32119078 [PubMed - as supplied by publisher]
Source: The Journal of Clinical Endocrinology and Metabolism - March 1, 2020 Category: Endocrinology Authors: Song Y, Su RW, Joshi NR, Kim TH, Lessey BA, Jeong JW, Fazleabas AT Tags: J Clin Endocrinol Metab Source Type: research

Resveratrol attenuates diabetes-associated cell centrosome amplification via inhibiting the PKC α-p38 to c-myc/c-jun pathway.
In conclusion, our results suggest that PKCα-p38 to c-myc/c-jun is the signaling pathway of the diabetes-associated centrosome amplification, and resveratrol attenuates the centrosome amplification by inhibiting this signaling pathway. PMID: 31844893 [PubMed - as supplied by publisher]
Source: Acta Biochimica et Biophysica Sinica - December 15, 2019 Category: Biochemistry Authors: Wu Q, Chen X, He Q, Lang L, Xu P, Wang P, Lee SC Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Yes-associated protein (YAP1) is required for proliferation and function of bovine granulosa cells in vitro$.
Abstract Yes-associated protein (YAP1) is a major component of the Hippo signaling pathway. Although the exact extracellular signals that control the Hippo pathway are currently unknown, increasing evidence supports a critical role for the Hippo pathway in embryonic development, regulation of organ size, and carcinogenesis. Granulosa cells within the ovarian follicle proliferate and produce steroids and growth factors, which facilitate growth of follicle and maturation of the oocyte. We hypothesize that YAP1 plays a role in proliferation and estrogen secretion of granulosa cells. In the current study, we examined ...
Source: Biology of Reproduction - July 26, 2019 Category: Reproduction Medicine Authors: Plewes MR, Hou X, Zhang P, Liang A, Hua G, Wood JR, Cupp AS, Lv X, Wang C, Davis JS Tags: Biol Reprod Source Type: research

Synergistic inhibition of csal1 and csal3 in granulosa cell proliferation and steroidogenesis of hen ovarian prehierarchical development.
This study aimed to explore the potential role and mechanism of csal1 and csal3 in granulosa cells (GCs) proliferation, differentiation, and follicle selection within the PFs of hen ovary. Our data demonstrated that the csal1 and csal3 transcriptions were highly expressed in GCs of the various-sized PFs and their proteins were mainly localized in the cytoplasm of granulosa cells and oocytes of the examined PFs as well as in the ovarian stroma and epithelium. It initially revealed that both csal1 and csal3 may be involved in chicken PF development via a translocation mechanism. Furthermore, our results showed an abundance o...
Source: Biology of Reproduction - July 26, 2019 Category: Reproduction Medicine Authors: Zhu H, Qin N, Xu X, Sun X, Chen X, Zhao J, Xu R, Mishra B Tags: Biol Reprod Source Type: research

Endogenous retrovirus-encoded Syncytin-2 contributes to exosome-mediated immunosuppression of T cells.
Abstract Modulation of the activation status of immune cell populations during pregnancy depends on placental villous cytotrophoblast (VCT) cells and the syncytiotrophoblast (STB) layer. Failure in the establishment of this immunoregulatory function leads to pregnancy complications. Our laboratory has been studying Syncytin-2 (Syn-2), an endogenous retroviral protein expressed in placenta and on the surface of placental exosomes. This protein plays an important role in STB formation through its fusogenic properties, but also possesses an active immunosuppressive domain (ISD). Considering that Syn-2 expression is i...
Source: Biology of Reproduction - July 17, 2019 Category: Reproduction Medicine Authors: Lokossou AG, Toudic C, Nguyen PT, Elisseeff X, Vargas A, Rassart É, Lafond J, Leduc L, Bourgault S, Gilbert C, Scorza T, Tolosa J, Barbeau B Tags: Biol Reprod Source Type: research

SMAD-dependent signaling mediates BMP6-induced stimulation of connective tissue growth factor in luteinized human granulosa cells.
In this study, we demonstrated that BMP6 treatment significantly increased the expression of CTGF in both primary and immortalized human granulosa cells. Using both pharmacological inhibitors and siRNA-mediated knockdown approaches, we showed that ALK2 and ALK3 type I receptors are required for BMP6-induced cellular activities. Furthermore, this effect is most likely mediated by a SMAD-dependent pathway. Our studies provide novel insight into the molecular mechanisms by which an intraovarian growth factor affects the production of another factor via a paracrine effect in human granulosa cells. PMID: 31210269 [PubMed -...
Source: Biology of Reproduction - June 17, 2019 Category: Reproduction Medicine Authors: Liu S, Chang HM, Yi Y, Yao YQ, Leung PCK Tags: Biol Reprod Source Type: research

YAP mediates the positive regulation of hnRNPK on the lung adenocarcinoma H1299 cell growth.
In this study, we observed that the expression pattern of hnRNPK in H1299 lung adenocarcinoma cells varied depending on the cell density in culture. Moreover, hnRNPK stimulated the ability of proliferation and colony formation of H1299 cells, which is important for the multilayered cell growth in culture. We further investigated whether there is an association between hnRNPK and the elements involved in the cell contact inhibition pathway. By using quantitative reverse transcriptase-polymerase chain reaction assay and a YAP activity reporter system, we found that hnRNPK upregulated the mRNA and protein levels and transcrip...
Source: Acta Biochimica et Biophysica Sinica - June 11, 2019 Category: Biochemistry Authors: Xu L, Zhang T, Huang W, Liu X, Lu J, Gao X, Zhang YF, Liu L Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research