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Cancer: Rhabdomyosarcoma

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Total 29 results found since Jan 2013.

Long non-coding RNA SNHG9 regulates viral replication in rhabdomyosarcoma cells infected with enterovirus D68 via miR-150-5p/c-Fos axis
ConclusionOur findings indicate that the SNHG9/miR-150-5p/c-Fos axis influences EV-D68 replication in host cells and that SNHG9 may be a possible target for anti-EV-D68 infection therapies.
Source: Frontiers in Microbiology - January 19, 2023 Category: Microbiology Source Type: research

Genes, Vol. 12, Pages 1373: High Frequency of Tumor Propagating Cells in Fusion-Positive Rhabdomyosarcoma
In this study we investigated the expression of key stem cell markers, developed a sphere assay, and investigated the seven most common FPRMS cell lines for subpopulations of tumor propagating cancer stem-like cells, also called cancer stem cells (CSCs). Moreover, loss- and gain-of-functions of the stem cell genes SOX2, OCT4, and NANOG were investigated in the same cells. Single-cell clonal analysis was performed in vitro as well as in vivo. We found that no stable CSC subpopulation could be enriched in FPRMS. Unlike depletion of PAX3-FOXO1, neither overexpression nor siRNA-mediated downregulation of SOX2, OCT4, and NANOG ...
Source: Genes - August 31, 2021 Category: Genetics & Stem Cells Authors: Melanie Generali Sampoorna Satheesha Peter K. Bode Debora Wanner Beat W. Sch äfer Elisa A. Casanova Tags: Article Source Type: research

Pharmacologic Inhibition of Ezrin-Radixin-Moesin Phosphorylation is a Novel Therapeutic Strategy in Rhabdomyosarcoma.
This study investigates the inhibition of ERM phosphorylation using a small molecule pharmacophore NSC668394 as a potential strategy against RMS. Upon in vitro treatment with NSC668394, RMS cells exhibit a dose-dependent decrease in cell viability and proliferation, with induction of caspase-3 cleavage and apoptosis. siRNA-mediated knockdown of individual ERM protein expression revealed that each regulates RMS survival to a different degree. In vivo administration of NSC668394 in RMS xenografts causes significant decrease in tumor growth, with no adverse effect on body weight. Collectively, this study highlights the import...
Source: Sarcoma - October 3, 2020 Category: Cancer & Oncology Tags: Sarcoma Source Type: research

Characterization of GRK5 as a novel regulator of rhabdomyosarcoma tumor cell growth and self-renewal.
Authors: Pham T, Robinson K, Vleeshouwer-Neumann T, Annis JE, Chen EY Abstract Rhabdomyosarcoma (RMS) is the most common soft-tissue pediatric sarcoma. Clinical outcomes for RMS patients with relapsed or metastatic disease remain poor. Treatment options remain limited, presenting an urgent need for novel therapeutic targets. Using a high-throughput siRNA screen against the human kinome, we identified GRK5, a G-protein receptor kinase, as a novel regulator of RMS tumor cell growth and self-renewal. Through functional assays in vitro and in vivo, we show that GRK5 regulates cell cycle in a kinase-independent manner t...
Source: Oncotarget - May 5, 2020 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

GSE140040 Next-generation sequencing reveals a novel role of lysine-specific demethylase 1 in adhesion of rhabdomyosarcoma cells RNA-seq
This study aims at elucidating changes in the RNA expression profile by RNA Sequencing in control and LSD1 knockdown conditions using transient silencing with siRNA. In addition, ChIP Sequencing was employed to investigate changes in the histone methylation pattern of H3K4me2 as a target of LSD1 in control and LSD1 knockdown conditions.
Source: GEO: Gene Expression Omnibus - November 7, 2019 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

GSE140039 Next-generation sequencing reveals a novel role of lysine-specific demethylase 1 in adhesion of rhabdomyosarcoma cells ChIP-seq
This study aims at elucidating changes in the RNA expression profile by RNA Sequencing in control and LSD1 knockdown conditions using transient silencing with siRNA. In addition, ChIP Sequencing was employed to investigate changes in the histone methylation pattern of H3K4me2 as a target of LSD1 in control and LSD1 knockdown conditions.
Source: GEO: Gene Expression Omnibus - November 7, 2019 Category: Genetics & Stem Cells Tags: Genome binding/occupancy profiling by high throughput sequencing Homo sapiens Source Type: research

Macropinocytosis Dependent Entry of Chikungunya Virus into Human Muscle Cells
This study shows for the first time, that the infectious entry of CHIKV into human muscle cells is mediated by macropinocytosis. Together, the da ta from this study may pave the way for the development of specific inhibitors that target the entry process of CHIKV into cells.
Source: PLoS Neglected Tropical Diseases - August 25, 2019 Category: Tropical Medicine Authors: Ching Hua, Regina Lee Source Type: research

Susceptibility of Enterovirus-D68 to RNAi-mediated antiviral knockdown
Publication date: Available online 20 July 2019Source: Antiviral ResearchAuthor(s): Nicholas Klaiber, Michael A. McVoy, Wei ZhaoAbstractEnterovirus D68 (EV-D68) represents an emerging pathogen which has demonstrated a capacity for causing epidemic illness in pediatric and immunocompromised patients. With no effective antiviral treatment available, therapeutic interventions are currently limited to supportive care. Utilizing available genomic sequences from the 2014 B3 Epidemic EV-D68 clade and the 1962 Fermon EV-D68 strains, we performed in silico comparative genomic analysis, identifying several islands of phylogenetic co...
Source: Antiviral Therapy - July 21, 2019 Category: Virology Source Type: research

Susceptibility of Enterovirus-D68 to RNAi-mediated antiviral knockdown.
Abstract Enterovirus D68 (EV-D68) represents an emerging pathogen which has demonstrated a capacity for causing epidemic illness in pediatric and immunocompromised patients. With no effective antiviral treatment available, therapeutic interventions are currently limited to supportive care. Utilizing available genomic sequences from the 2014 B3 Epidemic EV-D68 clade and the 1962 Fermon EV-D68 strains, we performed in silico comparative genomic analysis, identifying several islands of phylogenetic conservation within the viral RNA-dependent RNA polymerase gene. The effects of transfecting short-interfering double-st...
Source: Antiviral Research - July 19, 2019 Category: Virology Authors: Klaiber N, McVoy MA, Zhao W Tags: Antiviral Res Source Type: research

Complement C5b-9 and Cancer: Mechanisms of Cell Damage, Cancer Counteractions, and Approaches for Intervention
In conclusion, osmotic burst of inflated complement-damaged cells may occur, but these bursts are most likely a consequence of metabolic collapse of the cell rather than the cause of cell death. The Complement Cell Death Mediator: A Concerted Action of Toxic Moieties Membrane pores caused by complement were first visualized by electron microscopy on red blood cell membranes as large ring structures (22). Similar lesions were viewed on E. coli cell walls (23). Over the years, ample information on the fine ultrastructure of the MAC that can activate cell death has been gathered (24) and has been recently further examined (...
Source: Frontiers in Immunology - April 9, 2019 Category: Allergy & Immunology Source Type: research

Targeting ALK in pediatric RMS does not induce antitumor activity in vivo
ConclusionsWhile ALK appears to be a relevant target in RMS in vitro, targeting this kinase in vivo yields no therapeutic efficacy, warranting extreme caution when considering the use of these agents in pediatric RMS patients.
Source: Cancer Chemotherapy and Pharmacology - May 31, 2018 Category: Cancer & Oncology Source Type: research

Hedgehog signaling negatively co-regulates BH3-only protein Noxa and TAp73 in TP53-mutated cells
In the present study, we show that pharmacological repression by the Hedgehog (Hh) pathway inhibitor (HPI) GANT61 induces expression of the proapoptotic protein Noxa in TP53-mutated embryonal pediatric tumor cells driven by Hh signaling (i.e. rhabdomyosarcoma (RMS) and medulloblastoma (MB)). Similarly, genetic silencing of Gli1 by siRNA causes increased Noxa mRNA and protein levels, while overexpression of Gli1 results in decreased Noxa expression. Furthermore, TAp73 mRNA and protein levels are increased upon Gli1 knockdown, while Gli1 overexpression reduces TAp73 mRNA and protein levels.
Source: Cancer Letters - April 24, 2018 Category: Cancer & Oncology Authors: Michael Torsten Meister, Cathinka Boedicker, Thomas Klingebiel, Simone Fulda Tags: Original Articles Source Type: research

DUX4-induced dsRNA and MYC mRNA stabilization activate apoptotic pathways in human cell models of facioscapulohumeral dystrophy
by Sean C. Shadle, Jun Wen Zhong, Amy E. Campbell, Melissa L. Conerly, Sujatha Jagannathan, Chao-Jen Wong, Timothy D. Morello, Silv ère M. van der Maarel, Stephen J. Tapscott Facioscapulohumeral dystrophy (FSHD) is caused by the mis-expression of DUX4 in skeletal muscle cells. DUX4 is a transcription factor that activates genes normally associated with stem cell biology and its mis-expression in FSHD cells results in apoptosis. To identify genes and pathways necessary for DUX4-mediated apoptosis, we performed an siRNA screen in an RD rhabdomyosarcoma cell line with an inducibleDUX4 transgene. Our screen identified compon...
Source: PLoS Genetics - March 7, 2017 Category: Genetics & Stem Cells Authors: Sean C. Shadle Source Type: research

GSE87495 DUX4-induced dsRNA and MYC mRNA Stabilization Lead to Apoptosis in Human Cell Models of Facioscapulohumeral Dystrophy
Contributors : Sean C Shadle ; Jun W Zhong ; Amy E Campbell ; Melissa L Conerly ; Sujatha Jagannathan ; Chao-Jen Wong ; Timothy D Morello ; Stephen J TapscottSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensFacioscapulohumeral muscular dystophy (FSHD) is caused by the mis-expression of DUX4 in skeletal muscle cells. DUX4 is a transcription factor that activates genes normally associated with stem cell biology and its mis-expression in FSHD cells results in apoptosis. To identify genes and pathways necessary for DUX4-mediated apoptosis, we performed an siRNA screen in an RD rhabdomyosar...
Source: GEO: Gene Expression Omnibus - December 31, 2016 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research