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3-deazaneplanocin A, a histone methyltransferase inhibitor, improved the chemoresistance induced under hypoxia in melanoma cells
In conclusion, a histone methyltransferase EZH2 inhibitor, DZNep was capable of tackling acquired chemoresistance via the suppression of histone methylation induced under hypoxic tumor microenvironment.PMID:37542772 | DOI:10.1016/j.bbrc.2023.08.003
Source: Biochemical and Biophysical Research communications - August 5, 2023 Category: Biochemistry Authors: Mika Hosokawa Sekai Tetsumoto Mirano Yasui Yusuke Kono Ken-Ichi Ogawara Source Type: research

Expression and Purification of FGFR1-Fc Fusion Protein and Its Effects on Human Lung Squamous Carcinoma
Appl Biochem Biotechnol. 2023 May 9. doi: 10.1007/s12010-023-04542-6. Online ahead of print.ABSTRACTMolecular-targeted therapies for lung squamous cell carcinoma (LSCC) are limited mainly because targetable oncogenic aberrations are absent in LSCC. Recent genomic analyses have revealed that the fibroblast growth factor (FGF) signaling pathway plays a fundamental role in LSCC progression via cancer cell proliferation and angiogenesis. In the present study, we designed, expressed, and purified a fibroblast growth factor receptor fragment (FGFR1-Fc) fusion protein using NS/0 cells. In FGF2-FGFR1 overexpressed NCI-H1703 cells,...
Source: Applied Biochemistry and Biotechnology - May 9, 2023 Category: Biochemistry Authors: Lulu Zheng Huan Liu Lingfeng Chen Xinyi You Fangyi Lv Haibing Fan Qi Hui Baohua Liu Xiaojie Wang Source Type: research

ANXA2 as a novel substrate of FBXW7 promoting esophageal squamous cell carcinoma via ERK phosphorylation
In conclusion, this study provided evidence that FBXW7 loss of function could promote esophageal cancer through ANXA2 overexpression, and this novel regulation pathway may be used as an efficient target for ESCC treatment.PMID:36758484 | DOI:10.1016/j.bbrc.2023.01.082
Source: Biochemical and Biophysical Research communications - February 9, 2023 Category: Biochemistry Authors: Zongfei Li Yunzhi Pan Jiayi Yao Yingyin Gao Yulan Qian Minxue Zheng Sai Ma Source Type: research

Genetic Engineering of Dendritic Cells Using Partially Zwitterionic Dendrimer-Entrapped Gold Nanoparticles Boosts Efficient Tumor Immunotherapy
Biomacromolecules. 2022 Mar 2. doi: 10.1021/acs.biomac.1c01571. Online ahead of print.ABSTRACTEffective processing and cross-priming of tumor neoantigen by dendritic cells (DCs) to T cells for spontaneous immune response generation to effectively kill cancer cells remain challenging in cancer immunotherapy. Here, we report a general approach to genetically engineer DCs through silencing their YTHDF1 protein (an important reader protein responsible for RNA m6A methylation) expression via a dendrimeric non-viral vector to boost effective tumor immunotherapy. Poly(amidoamine) dendrimers of generation 5 were partially decorate...
Source: Biomacromolecules - March 2, 2022 Category: Biochemistry Authors: Zhijun Ouyang Yue Gao Rui Yang Mingwu Shen Xiangyang Shi Source Type: research

The MDM2 ligand Nutlin-3 differentially alters expression of the immune blockade receptors PD-L1 and CD276.
Conclusion: Genetic inactivation of TP53, or the use of the MDM2 ligand Nutlin-3, alters the expression of the immune blockade receptors PD-L1 and CD276. The biological function of elevated CD276 is to promote altered cell cycle progression in response to Nutlin-3, whilst the major effect of elevated PD-L1 is T-cell suppression. These data indicate that TP53 gene status, ATM and MDM2 influence PD-L1 and CD276 paralogs on the cell surface. These data have implications for the use of drugs that target the p53 pathway as modifiers of immune checkpoint receptor expression. PMID: 32874188 [PubMed - in process]
Source: Cellular and Molecular Biology Letters - September 4, 2020 Category: Biochemistry Authors: Li R, Zatloukalova P, Muller P, Gil-Mir M, Kote S, Wilkinson S, Kemp AJ, Hernychova L, Wang Y, Ball KL, Tao K, Hupp T, Vojtesek B Tags: Cell Mol Biol Lett Source Type: research

Evaluating the effect of siRNA on sox2ot expression in the Human Neuron-Committed Teratocarcinoma NT2 Cellline.
Authors: Sadeghi Z, Dodange F, Maleki P, Zarei M, Taheri M, Raheb J Abstract Non-coding RNA elongated (lncRNAs) have recently attracted as molecules that regulate gene expression of the pluripotent properties (pluripotency) of stem cells. Recently our colleagues examined the role of one of these RNAs called SOX2OT in esophageal squamous cell carcinoma, and found a concomitant increase in its expression with some regulatory genes of cell proliferation. In the present study, using the design of suitable primers from SOX2OT gene, we investigated the effect of siRNA on expression of SOX2OT. PMID: 32716352 [PubMed -...
Source: Human Antibodies - July 29, 2020 Category: Biochemistry Tags: Hum Antibodies Source Type: research

BRAFV600E dictates cell survival via c-Myc-dependent induction of Skp2 in human melanoma.
In this study, we demonstrate that BRAFV600E drives cell growth and glycolysis in melanoma cells but does so by a previously unappreciated mechanism that involves direct induction of Skp2. Skp2 is highly expressed in melanoma tissues and particularly in tissues with BRAFV600E mutation. The inhibition of BRAFV600E by either siRNA or inhibitor vemurafenib suppressed Skp2 expression and cell growth. Mechanistic study shows that BRAFV600E suppression of Skp2 is dependent on c-Myc transcription factor via specifically bounding to the E-box region on SKP2 promoter. Further, the overexpression of Skp2 resulted in a markedly incre...
Source: Biochemical and Biophysical Research communications - January 20, 2020 Category: Biochemistry Authors: Feng L, Li J, Bu X, Zuo Y, Shen L, Qu X Tags: Biochem Biophys Res Commun Source Type: research

Alkylation enhances biocompatibility and siRNA delivery efficiency of cationic curdlan nanoparticles.
Abstract Cationic curdlan derivatives are a class of promising carriers for nucleic acid delivery including short interfering RNA (siRNA). While our previous studies demonstrated the siRNA delivery efficiency of aminated curdlan derivatives, the associated cytotoxicity issue remained unsolved. To investigate the effects of alkylation on the toxicity as well as the transfection efficiency, we conjugated short alkyl chains to 6-amino-6-deoxy-curdlan (6AC-100). The cytotoxicity of alkylated 6AC-100 derivatives (denote CuVa polymers) decreased with the increase of the degree of substitution (DS). CuVa3, with the highe...
Source: International Journal of Biological Macromolecules - December 5, 2019 Category: Biochemistry Authors: Erdene-Ochir T, Ganbold T, Zandan J, Han S, Borjihan G, Baigude H Tags: Int J Biol Macromol Source Type: research

Downregulation of nicotinamide N-methyltransferase inhibits migration and epithelial-mesenchymal transition of esophageal squamous cell carcinoma via Wnt/ β-catenin pathway.
Downregulation of nicotinamide N-methyltransferase inhibits migration and epithelial-mesenchymal transition of esophageal squamous cell carcinoma via Wnt/β-catenin pathway. Mol Cell Biochem. 2019 Jul 05;: Authors: Cui Y, Zhang L, Wang W, Ma S, Liu H, Zang X, Zhang Y, Guan F Abstract Nicotinamide N-methyltransferase (NNMT) is an important methyltransferase involved in the biotransformation of many drugs and exogenous compounds. Abnormal expression of NNMT protein is closely associated with the onset and progression of many malignancies, but little is known about its role in esophageal squamous cell ca...
Source: Molecular and Cellular Biochemistry - July 4, 2019 Category: Biochemistry Authors: Cui Y, Zhang L, Wang W, Ma S, Liu H, Zang X, Zhang Y, Guan F Tags: Mol Cell Biochem Source Type: research

Voltage-gated sodium channel Nav1.5 promotes proliferation, migration and invasion of oral squamous cell carcinoma.
In this study, we found that Nav1.5 was highly expressed in OSCC cell lines compared with normal oral keratinocyte HOK cell line by using western blot analysis. CCK-8 assay results revealed that downregulation of Nav1.5 expression by its specific siRNA reduced proliferation of OSCC HSC-3 cells. Moreover, transwell assay results showed Nav1.5 knockdown significantly inhibited migration and invasion of HSC-3 cells. Meanwhile, qRT-PCR and western blot analysis results showed that epidermal growth factor (EGF) induced Nav1.5 expression in a time- and dose-dependent manner. In addition, EGF promoted proliferation, migration and...
Source: Acta Biochimica et Biophysica Sinica - May 28, 2019 Category: Biochemistry Authors: Zhang J, Mao W, Dai Y, Qian C, Dong Y, Chen Z, Meng L, Jiang Z, Huang T, Hu J, Luo P, Korner H, Jiang Y, Ying S Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Delivery of MTH1 inhibitor (TH287) and MDR1 siRNA via hyaluronic acid-based mesoporous silica nanoparticles for oral cancers treatment
Publication date: 1 January 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 173Author(s): Xiao-Lei Shi, Yuan Li, Lu-Ming Zhao, Lin-Wang Su, Gang DingAbstractOral cancer accounts for 95% of all maxillofacial malignant neoplasm. Presently, surgery and radiation (with and without chemotherapy) are the major treatments for oral cancer; however it met with limited therapeutic outcome. Overcoming the multidrug resistance and finding new therapeutic agents for oral cancer treatment are some of the serious challenges. Small molecule, TH287 potently and selectively inhibits the MTH1 protein in cells and could act as a ne...
Source: Colloids and Surfaces B: Biointerfaces - October 23, 2018 Category: Biochemistry Source Type: research

The oncolytic virus H101 combined with GNAQ siRNA ‐mediated knockdown reduces uveal melanoma cell viability
Journal of Cellular Biochemistry, EarlyView.
Source: Journal of Cellular Biochemistry - October 15, 2018 Category: Biochemistry Authors: Yongyun Li, Jie He, Chun Qiu, Qingfeng Shang, Guanxiang Qian, Xianqun Fan, Shengfang Ge, Renbing Jia Source Type: research

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