Association between HLA alleles and risk of celiac disease in Iranian patients.
In this study, 40 patients of CD and 40 healthy control samples were genotyped for HLA-DQB1 and 14 patients of CD and 14 healthy control samples were genotyped for HLA-DQA1genes using the SSP-PCR technique and a commercial kit.The DQA1*05 allele had the highest frequency among the patient group (42.86%). The frequency of this allele was 28.57% in healthy controls, and there was no statistically significant difference in this case (p= 0.771).The DQB1*02 allele was the most common in patients (33.75%) followed by the DQB1*03 allele (31.25%).The difference in frequency of the HLA-DQB1*02 allele in the patient and control grou...
Source: Human Antibodies - October 30, 2019 Category: Biochemistry Tags: Hum Antibodies Source Type: research

Optimized protocol for soluble prokaryotic expression, purification and refolding of the human inhibin α subunit, a cysteine rich peptide chain.
Optimized protocol for soluble prokaryotic expression, purification and refolding of the human inhibin α subunit, a cysteine rich peptide chain. Hum Antibodies. 2019 Oct 18;: Authors: Kolivand S, Nazari M, Modarressi MH, Najafabadi MRH, Hemati A, Ghafouri-Fard S, Motevaseli E Abstract BACKGROUND: Inhibin A, a member of TGF-β superfamily, consists of α and β subunits. These subunits contain several cysteine residues in amino acid sequence that forms inter- and intra-subunits disulfide bonds. Due to the reducing environment of the bacterial cytoplasm, disulfide bonds formation in E....
Source: Human Antibodies - October 30, 2019 Category: Biochemistry Tags: Hum Antibodies Source Type: research

The modified vaccination technique designed to prevent and cure acute and chronic disorders.
Authors: Barabas AZ, Cole CD, Kovacs ZB, Kovacs EI, Lafreniere R, Weir DM Abstract In spite of enormous efforts there have been no solutions to date for preventing/terminating certain acute and chronic disorders of humans by vaccination or drugs. Yet it is well understood that if the target antigen (ag) could be presented appropriately to the cells of the immune system then solutions could be found. Recently, the Barabas research group has introduced and described the third vaccination method - called modified vaccination technique (MVT) - which has the ability to provide a corrective immune response in experimenta...
Source: Human Antibodies - October 10, 2019 Category: Biochemistry Tags: Hum Antibodies Source Type: research

Association between tumor necrosis factor-alpha gene rs1800629 (-308G/A) and rs361525 (-238G > A) polymorphisms and prostate cancer risk in an Iranian cohort.
CONCLUSIONS: Although, the achieved results of this investigation demonstrated that the two examined genetic variants do not seem to be suitable markers for early diagnosis of prostate cancer in this pilot study; however increased risk for the disease is shown in GA heterozygotes and smokers which is indicative of some epigenetic factors influence on prostate cancer etiology. PMID: 31594215 [PubMed - as supplied by publisher] (Source: Human Antibodies)
Source: Human Antibodies - October 10, 2019 Category: Biochemistry Tags: Hum Antibodies Source Type: research

Assessment the genes and molecular mechanisms of B cells activation through systems biology approaches.
In conclusion, we explored holistic methods for molecular assay of difference between B cell activation mechanisms with Th1 and Th2. PMID: 31524152 [PubMed - as supplied by publisher] (Source: Human Antibodies)
Source: Human Antibodies - September 18, 2019 Category: Biochemistry Tags: Hum Antibodies Source Type: research

The relationship between health literacy and quality of life among hemodialysis patients: An integrative review.
CONCLUSION: There is a relationship between health literacy and quality of life among patients receiving hemodialysis. Conclusively, addressing health literacy may improve the quality of life among patients receiving hemodialysis. PMID: 31524153 [PubMed - as supplied by publisher] (Source: Human Antibodies)
Source: Human Antibodies - September 18, 2019 Category: Biochemistry Tags: Hum Antibodies Source Type: research

The nine lives of epitope-based antibody patents.
This article strives to give an overview about the as is situation both in Europe and the United States. PMID: 31524154 [PubMed - as supplied by publisher] (Source: Human Antibodies)
Source: Human Antibodies - September 18, 2019 Category: Biochemistry Tags: Hum Antibodies Source Type: research

Former antiplatelet drug administration and consequences of intravenous thrombolysis in acute ischemic stroke.
CONCLUSION: Based on the results of this study, patients with acute ischemic stroke and history of taking antiplatelet drugs have poor outcome following administration of standard dose of tPA. PMID: 31356199 [PubMed - as supplied by publisher] (Source: Human Antibodies)
Source: Human Antibodies - July 31, 2019 Category: Biochemistry Tags: Hum Antibodies Source Type: research

Cellular immune responses against cancer-germline genes in cancers.
CONCLUSION: Cellular immune responses against cancer-germline genes are indicative of appropriateness of these genes as therapeutic targets. PMID: 31356200 [PubMed - as supplied by publisher] (Source: Human Antibodies)
Source: Human Antibodies - July 31, 2019 Category: Biochemistry Tags: Hum Antibodies Source Type: research

Nanobodies: The "magic bullets" in therapeutics, drug delivery and diagnostics.
Nanobodies: The "magic bullets" in therapeutics, drug delivery and diagnostics. Hum Antibodies. 2019 Jul 12;: Authors: Mir MA, Mehraj U, Sheikh BA, Hamdani SS Abstract Antibodies represent a well-established class of clinical diagnostics for medical applications as well as essential research and biotechnological tools. Although both polyclonal and monoclonal antibodies are indispensable reagents in basic research and diagnostics but both of them have their limitations. Hence, there is urgent need to develop strategies aimed at production of alternative scaffolds and recombinant antibodies of ...
Source: Human Antibodies - July 21, 2019 Category: Biochemistry Tags: Hum Antibodies Source Type: research

The association between diet quality and obesity-related metabolic risks.
CONCLUSIONS: High diet quality incorporating more anti-inflammatory diet may have a potential benefit in reducing obesity-related metabolic risks. PMID: 31282409 [PubMed - as supplied by publisher] (Source: Human Antibodies)
Source: Human Antibodies - July 11, 2019 Category: Biochemistry Tags: Hum Antibodies Source Type: research

Anaemia and thrombocytopenia among pregnant women attending Aminu Kano Teaching Hospital, Kano State, North Western Nigeria.
CONCLUSION: The values obtained from this research showed an increase in prevalence of anaemia and thrombocytopenia among pregnant women compared to the non-pregnant controls. It is vital to routinely monitor the indices of anaemia and thrombocytopenia among pregnant women to reduce the incidence of these diseases and of their complications. PMID: 31282410 [PubMed - as supplied by publisher] (Source: Human Antibodies)
Source: Human Antibodies - July 11, 2019 Category: Biochemistry Tags: Hum Antibodies Source Type: research

Effects of malaria parasitaemia on some haemostatic parameters among pregnant women of African descent in Specialist Hospital Sokoto, Nigeria.
CONCLUSION: This study has shown that malaria in pregnancy causes a significant decrease in the platelet count and prolongation in the prothrombin (PT) and the activated partial thromboplastin time (APTT). There is need for the malaria and haemostatic parameters to be assayed routinely on pregnant women particularly those presenting to antenatal clinic with febrile illness. PMID: 31282411 [PubMed - as supplied by publisher] (Source: Human Antibodies)
Source: Human Antibodies - July 11, 2019 Category: Biochemistry Tags: Hum Antibodies Source Type: research

Assessment of SGO1 and SGO1-AS1 contribution in breast cancer.
Authors: Nasim N, Ghafouri-Fard S, Soleimani S, Esfandi F, Shirkhoda M, Safaei M, Oskooei VK, Taheri M, Raheb J Abstract Shugoshin-like protein 1 (SGO1) participated in the proper progression of mitosis. This fundamental role has indicated the importance of this gene in the pathogenesis of cancer as a disorder of mitotic cell division. A previous high throughput study of long non-coding RNAs (lncRNAs) expression in lung cancer has identified aberrant expression of SGO1-antisense 1 (SGO1-AS1) in these specimens. In the current study, we quantified expression of SGO1 and SGO1-AS1 in 39 breast cancer tissues and their...
Source: Human Antibodies - June 4, 2019 Category: Biochemistry Tags: Hum Antibodies Source Type: research

Comparison between the effect of regular human insulin and NPH with novo-rapid and levemir insulin in glycemic control in gestational diabetes.
CONCLUSION: Altogether, The Novo-rapid and Levemir Insulin in comparing with the Regular and NPH Insulin were practically advantageous due to the simple using method and short hospitalization period of the patient. Thus, we prefer and suggest this beneficial method (using Novo-rapid and Levemir Insulin) to reach therapeutic goals. PMID: 31156155 [PubMed - as supplied by publisher] (Source: Human Antibodies)
Source: Human Antibodies - June 4, 2019 Category: Biochemistry Tags: Hum Antibodies Source Type: research

Menstrual cycle-associated effects on some acute phase reactants parameters of students in Usmanu Danfodiyo University Sokoto, North Western Nigeria.
CONCLUSIONS: There is need to generate data on menstrual disorders and their impact on women's health status, quality of life and social integration. It is vital that evaluation and treatment of menstrual complaints should be given a higher priority in primary care programs. There is need to invest in public enlightenment program to increase awareness in secondary schools to increase the level of awareness among adolescents as well as young females. PMID: 31156156 [PubMed - as supplied by publisher] (Source: Human Antibodies)
Source: Human Antibodies - June 4, 2019 Category: Biochemistry Tags: Hum Antibodies Source Type: research

Effects of resveratrol supplementation in male Wistar rats undergoing an endurance exercise and acute exercise training.
CONCLUSION: This study provides further evidence of the antioxidant effects of RES after exercise. However, several factors such as type and duration of exercise, the type of model, the amount of RES supplementation and the time-course consideration can affect the quality of the results. For this reason, further studies in this field are required. PMID: 31127758 [PubMed - as supplied by publisher] (Source: Human Antibodies)
Source: Human Antibodies - May 29, 2019 Category: Biochemistry Tags: Hum Antibodies Source Type: research

Serum inducible protein-10 chemokine as a biomarker for clearance of HCV with and without treatment in Egyptian patients.
Authors: Tabll A, El Shenawy R, Elsharkawy H, Mohamed FZ Abstract BACKGROUND AND AIMS: Assessment the potential predictive value of serum inducible protein-10 chemokine (IP-10) in the clearance of HCV in Egyptian patients with and without treatment. MATERIALS AND METHODS: Ninety Egyptian individuals were involved in the current study where, 20 patients (23%) were chronic HCV (positive HCV antibodies and positive HCV RNA without treatment, 20 (22%) were healthy individuals (negative for both HCV antibodies and HCV RNA, 20 (22%) were natural clearance (positive HCV antibodies and negative for HCV RNA without trea...
Source: Human Antibodies - May 29, 2019 Category: Biochemistry Tags: Hum Antibodies Source Type: research

Leydig cells express the FABP9 in human testis.
CONCLUSION: The expression of FABP9 in a different cell type rather than spermatogenic cells in other mammals, reports of a plausible different function for the gene product like its involvement in fertility potential in homo sapiens. PMID: 31127760 [PubMed - as supplied by publisher] (Source: Human Antibodies)
Source: Human Antibodies - May 29, 2019 Category: Biochemistry Tags: Hum Antibodies Source Type: research

Perceptions of leadership, motivation, structure, and assurance for implementation of emergency medical services in Ethiopia: Perspectives of emergency medical services case teams based on focus group discussions.
Authors: Gebru AA, Mosadeghrad AM, Sari AA Abstract BACKGROUND: The research was assessed the Ethiopian Emergency Medical Services Case teams' and officers views on Emergency Medical services in Ethiopia. The aim of study was to present the point of views, prospect and priorities of Emergency medical services case teams and its coordinator along some main dimensions of emergency medical services, such as Leadership, Motivation, Structure, and Assurance for implementation. METHODS: Six focus group discussion were facilitated with EMS case teams, focal persons and FMoH emergency medical services case teams from D...
Source: Human Antibodies - May 29, 2019 Category: Biochemistry Tags: Hum Antibodies Source Type: research

Some haematological parameters among pregnant women of African descent attending antenatal clinic in Sokoto North Western Nigeria.
Authors: Polycarp II, Osaro E, Kester D Abstract Pregnancy is the fertilization and development of one or more offspring, known as an embryo or foetus in a woman's uterus. Pregnancy is a critical stage of development during which maternal nutrition can strongly influence obstetric and neonatal outcomes. The aim of this study was to determine the effect of pregnancy on the full blood count parameters of pregnant women of African descent residing in Sokoto, North Western Nigeria. This case-control study investigated 74 pregnant subjects and 22 non-pregnant controls. The mean age and income of the subjects w...
Source: Human Antibodies - May 4, 2019 Category: Biochemistry Tags: Hum Antibodies Source Type: research

Estimating the household drought driven food insecurity using system dynamics model: The case of afar national regional state of Ethiopia.
Authors: Belay GD, Ostadtaghizadeh A, Khoei EM, Ardalan A, Hosseinzadeh-Attar MJ, Assen M Abstract BACKGROUND: Building resilient communities has emerged as a dominant schema in the policy arena and in academia in the wake of recent disasters. Food insecurity is a complex and multidimensional phenomenon that is not appropriately captured by conventional proxy indicators currently used to assess the problem in developing countries. Ethiopia like many countries in sub-Saharan Africa, continues to experience high levels of drought driven food insecurity despite decades of implementing poverty alleviation and preventio...
Source: Human Antibodies - April 24, 2019 Category: Biochemistry Tags: Hum Antibodies Source Type: research

Humoral immune responses against cancer-testis antigens in human malignancies.
CONCLUSION: Humoral immune responses against CTAs are putative biomarkers for cancer detection and follow-up. PMID: 31006681 [PubMed - as supplied by publisher] (Source: Human Antibodies)
Source: Human Antibodies - April 24, 2019 Category: Biochemistry Tags: Hum Antibodies Source Type: research

Client satisfaction on Emergency department services and quality of emergency medical care in Ethiopia: A systematic review.
CONCLUSION: This study finding shown that there was the variety of client perception on the emergency department and its quality care. Therefore, Emergency outpatient department should be raised progressively further by health care managers, governmental executives, EMS directorate, and other to address the efficient attribute of deprived value of health care and continuity of care delivery system but also it should be tied with new policy in Ethiopia. PMID: 30958338 [PubMed - as supplied by publisher] (Source: Human Antibodies)
Source: Human Antibodies - April 9, 2019 Category: Biochemistry Tags: Hum Antibodies Source Type: research

Emergency medical services in Ethiopia: Drivers, challenges and opportunities.
CONCLUSION: A long-term discussion is needed to further services system in various health care facilities. An Emergency Medical services policy making and analysis framework is needed to make quality emergency medical care at Emergency department in hospitals and outside the hospitals. PMID: 30958339 [PubMed - as supplied by publisher] (Source: Human Antibodies)
Source: Human Antibodies - April 9, 2019 Category: Biochemistry Tags: Hum Antibodies Source Type: research

Impact of food insecurity and malnutrition on the burden of Non-communicable diseases and death in Ethiopia: A situational analysis.
CONCLUSION: The SDGs are a target to call for comprehensive and it should have participatory approaches worldwide. Therefore, Ethiopian government and all policy-makers should be given great attention in order to improve the overall problems in the country. Especially, it requires the knowledge, commitment and responsibilities of politicians, researchers, health professionals and all stakeholders in the country. PMID: 30958340 [PubMed - as supplied by publisher] (Source: Human Antibodies)
Source: Human Antibodies - April 9, 2019 Category: Biochemistry Tags: Hum Antibodies Source Type: research

Establishment of serum derived infectivity coculture model for enhancement of hepatitis C virus replication in vitro.
CONCLUSIONS: Viral replication capacity was increased 3-5 folds in the coculture condition compared to the individual cell lines, which indicates an improvement to viral infectivity in vitro. SIGNIFICANCE STATEMENT: This novel coculture system represents a new in vitro model that will help study the underlying mechanisms of HCV pathogenicity. PMID: 30958341 [PubMed - as supplied by publisher] (Source: Human Antibodies)
Source: Human Antibodies - April 9, 2019 Category: Biochemistry Tags: Hum Antibodies Source Type: research

Assessment of malnutrition and its anthropometric measurement among 0-59 month children aged at Amibara and Awash Fentale districts, afar national regional state of Ethiopia.
Authors: Hosseinzadeh Attar MJ, Belay GD, Ardalan A, Assen M, Khoei EM, Ostadtaghizadeh A Abstract PURPOSE: Famines and food shortages are one of the most common long time histories of Ethiopia. Hence, malnutrition has been persisted at alarming raters in the country. Moreover, it is a major cause of morbidity and mortality in Afar specifically, Amibara and Awash Fentale districts. The anthropometric data were presented on height, weight, MUAC, weight/height/Age and derived measure of Body Mass Index (BMI) of under-five aged children's. METHODS: A cross-sectional descriptive survey was conducted from March 01, ...
Source: Human Antibodies - April 9, 2019 Category: Biochemistry Tags: Hum Antibodies Source Type: research

Circulating innate and adaptive immunity against anti-Haemophilus influenzae type b.
Authors: Zarei AE, Linjawi MH, Redwan EM Abstract Haemophilus influenzae type b (Hib) are one of most dangerous microbes that occupies the paediatric nasopharyngeal as a commensal opportunistic bacterium, which may lead to meningitis in uncontrolled infection. Colonisation of pharyngeal tissues is the starting point for most H. influenzae infections, which may develop into invasive diseases, such meningitis. The vaccination against Hib in specific, as well as against most of vaccines preventable diseases; in general, play a major role in reducing children (
Source: Human Antibodies - April 9, 2019 Category: Biochemistry Tags: Hum Antibodies Source Type: research

Short note: Infliximab recovery in a simulated intestinal fluid of the upper intestine tract.
Authors: Volpatti D, Gulisano E, Spanghero M Abstract BACKGROUND: The oral administration of Infliximab (IFX) antibody would ensure a direct action on inflammatory intestinal tissues without side effects. Thus, investigations about its resilience within the intestinal environment are required. OBJECTIVE: Quantify the IFX recovery in a simulated upper intestinal environment. METHODS: IFX was incubated for different times until 120 min in simulated intestinal fluids (SIF) which differed (i) for pH (7.2 vs 6.8, Exp 1), (ii) for addition or not with pancreatin (Exp 2) and (iii) for addition or not with bov...
Source: Human Antibodies - April 9, 2019 Category: Biochemistry Tags: Hum Antibodies Source Type: research

Some haematological parameters, copper and selenium level among children of African descent with sickle cell disease in specialist hospital Sokoto, Nigeria.
Authors: Erhabor O, Ogar K, Tosan E, Dangana A Abstract BACKGROUND: Sickle cell disease is a genetic disorder of haemoglobin causing myriad of pathology including anaemia. OBJECTIVES: The aim of this study was to evaluate some haematological parameters and trace elements of total of forty-five (45) children with Sickle cell disease attending Specialist Hospital Sokoto. METHOD: Twenty-five (25) apparently healthy children which were assessed as controls. The haematological parameters were determined using automated method and trace elements (copper and selenium) were determined using colorimetric and atomic ...
Source: Human Antibodies - March 26, 2019 Category: Biochemistry Tags: Hum Antibodies Source Type: research

Morbidity, mortality and antimicrobial resistance of pneumococcal infections in the Jamaican paediatric and adult populations.
CONCLUSIONS: The high burden of IPD in at-risk groups in our population and the associated increase in morbidity and mortality underlie the need for improved preventive and therapeutic management strategies in these patients. PMID: 30909200 [PubMed - as supplied by publisher] (Source: Human Antibodies)
Source: Human Antibodies - March 26, 2019 Category: Biochemistry Tags: Hum Antibodies Source Type: research

Dys-regulation of peripheral transcript levels of ecto-5'-nucleotidase in multiple sclerosis patients.
Authors: Kobarfard F, Noroozi R, Beirami AD, Taheri M, Ghafouri-Fard S Abstract CD73, also entitled as ecto-5'-nucleotidase (NT5E), is an ecto-nucleotidase that contributes in the breakage of extracellular ATP to adenosine and the preservation of immune balance. In spite of acknowledged role for immune response imbalance in the pathogenesis of multiple sclerosis (MS), data regarding NT5E expression in MS patients are scarce. In the current study, we assessed expression of NT5E in peripheral blood of MS patients and healthy subjects to unravel its role in the pathogenesis of MS. Results of Multilevel Bayesian model ...
Source: Human Antibodies - March 26, 2019 Category: Biochemistry Tags: Hum Antibodies Source Type: research

Prevalence, pattern, magnitude and associated factors of trauma in the Emergency Department at Health Institutes in Ethiopia: A systematic review.
Authors: Gebru AA, Mosadeghrad AM, Sari AA, Tafesse TB, Kahsay WG Abstract BACKGROUND: We aimed to identify and describe the prevalence, patterns, magnitude and associated factors of trauma in the Emergency Department at Health Institute in Ethiopia. METHODS: In this systematic review we searched for peer-reviewed and grey literature publications reporting the prevalence, pattern, magnitude and associated factors of trauma between 2000 to 2019. The documents which recruited are directly related to trauma and emergency department. In this regard, we searched databases of PubMed, Elsevier, Science directed and ME...
Source: Human Antibodies - March 26, 2019 Category: Biochemistry Tags: Hum Antibodies Source Type: research

Impact of climate change on community health and resilience in Ethiopia: A review article.
This study aimed to determine and describe community health status through building drought resilience in Ethiopia. METHODS: This review was conducted based on the available peer-reviewed articles that were published from January 2004 to December 2018 Online databases from PubMed, MEDLINE, Scopus, EMBASE and Google Scholar were searched for studies related to the building drought and community health resilience at different regions of Ethiopia that were published in English language. The search was made by using "Emergency disaster", "prevention", "preparedness", "response", &quo...
Source: Human Antibodies - March 26, 2019 Category: Biochemistry Tags: Hum Antibodies Source Type: research

Pregnancy outcomes of the patients with anti smooth muscle antibody positivity.
In this study, 40 ASMA positive patient were evaluated and compared with 80 control cases. Gestational week at delivery, birthweight and APGAR scores fort the 1st, 5th, 10th were significantly lower at patients with ASMA positivity (p
Source: Human Antibodies - March 26, 2019 Category: Biochemistry Tags: Hum Antibodies Source Type: research

Cancer-testis antigens: An update on their roles in cancer immunotherapy.
CONCLUSIONS: Based on the availability of different formulations of CTA-based vaccines, future researches should compare efficiency of these modalities. PMID: 30909205 [PubMed - as supplied by publisher] (Source: Human Antibodies)
Source: Human Antibodies - March 26, 2019 Category: Biochemistry Tags: Hum Antibodies Source Type: research

Blood group antigen-Binding Adhesion2 (BabA2) gene in gastric tissue biopsies as a diagnostic biomarker for Helicobacter pylori infection.
CONCLUSION: BabA2 gene detection in gastric tissue biopsies could be suggested as a diagnostic biomarker to be included among the other biomarkers routinely performed for clinical diagnosis of H. pylori infection. PMID: 30909206 [PubMed - as supplied by publisher] (Source: Human Antibodies)
Source: Human Antibodies - March 26, 2019 Category: Biochemistry Tags: Hum Antibodies Source Type: research

Long non-coding RNAs: Diverse roles in various disorders.
CONCLUSIONS: Taken together, lncRNAs constitute a novel vast area of research to find answer to fundamental biologic questions. PMID: 30909207 [PubMed - as supplied by publisher] (Source: Human Antibodies)
Source: Human Antibodies - March 26, 2019 Category: Biochemistry Tags: Hum Antibodies Source Type: research

Some haematological parameters, manganese and zinc levels among diabetic patients of African descent attending Specialist Hospital Sokoto, Nigeria.
CONCLUSIONS There is need to routinely monitor the full blood count, zinc and manganese levels among diabetic subjects in the area to optimize the care offered to these patients. PMID: 30909208 [PubMed - as supplied by publisher] (Source: Human Antibodies)
Source: Human Antibodies - March 26, 2019 Category: Biochemistry Tags: Hum Antibodies Source Type: research

Expression analysis of Inhibitor Of DNA Binding 1 (ID-1) gene in breast cancer.
CONCLUSION: ID-1 expression may be implicated in the pathogenesis of breast cancer especially in patient with ER negative status. PMID: 30856107 [PubMed - as supplied by publisher] (Source: Human Antibodies)
Source: Human Antibodies - March 13, 2019 Category: Biochemistry Tags: Hum Antibodies Source Type: research

Impact of extractable nuclear antigen, anti-double stranded DNA, antiphospholipid antibody, and anticardiolipin antibody positivity on obstetrical complications and pregnancy outcomes.
Authors: Tanacan A, Sinan Beksac M, Orgul G, Duru S, Sener B, Karaagao E Abstract BACKGROUND: Extractable nuclear antigen (ENA) and anti-double stranded DNA (anti-dsDNA) positivity and related diseases like systemic lupus erythematosus, Sjögren syndrome, and other autoimmune diseases are known to be associated with obstetrical complications and poor perinatal outcomes. OBJECTIVE: To demonstrate the importance of ENA, anti-dsDNA, antiphospholipid (APL), and anticardiolipin (ACL) antibody positivity on pregnancy outcomes. METHODS: Ninety one pregnant women with known ENA, anti-dsDNA, APL IgG and IgM, and...
Source: Human Antibodies - March 13, 2019 Category: Biochemistry Tags: Hum Antibodies Source Type: research

Investigation of LPA sequence variants rs6415084, rs3798220 with conventional coronary artery disease in Iranian CAD patients.
CONCLUSION: Our data did not indicate a relationship between genomic LPA variants (rs10455872 and rs3798220) and subsequent cardiovascular events in Iranian CAD patients. We did not confirm the association of the theses SNPs with CAD in our samples of Iranian patients. For the studied variants, our finding is consistent with reports which showed the lack of this genetic association in other populations. PMID: 30594920 [PubMed - as supplied by publisher] (Source: Human Antibodies)
Source: Human Antibodies - January 1, 2019 Category: Biochemistry Tags: Hum Antibodies Source Type: research

Safety and tolerability of mice to repeated subcutaneous injections of a peptide mix as a potential vaccine against HCV infection.
CONCLUSIONS: The extravagant histopathological changes in different organs have exiled the 16 μg dose out of acceptable range and validated that Cenv6 is safe and tolerable at the two lower doses (800 and 1600 ng/25 gm bw). PMID: 30594921 [PubMed - as supplied by publisher] (Source: Human Antibodies)
Source: Human Antibodies - January 1, 2019 Category: Biochemistry Tags: Hum Antibodies Source Type: research

Management and the pregnancy outcomes of patients positive for anti-parietal cell antibody.
CONCLUSIONS: Patients with more than one autoantibody positivity, including APCA, must be considered as high-risk patients. PMID: 30594922 [PubMed - as supplied by publisher] (Source: Human Antibodies)
Source: Human Antibodies - January 1, 2019 Category: Biochemistry Tags: Hum Antibodies Source Type: research

Altered methylation and expression patterns of genes regulating placental nitric oxide pathway in patients with severe preeclampsia.
Authors: Azizi F, Omrani MD, Amiri V, Mirfakhraie R, Dodangeh F, Shahmirzadi SA, Gargari SS Abstract Pre-eclampsia is a common pregnancy disorder syndrome whose molecular mechanism is not clear. Nitric oxide (NO) is a key regulator of placentation. Reduction of NO has previously been associated with endothelial dysfunction in pre-eclamptic women. Therefore, we measured expression and methylation of some placental genes that were involved in NO pathway like named ARG II, PRMT1 and DDAH2 in pre-eclampsia and normal pregnancies in order to determine whether impairment of expression of these genes in the pre-eclamptic ...
Source: Human Antibodies - January 1, 2019 Category: Biochemistry Tags: Hum Antibodies Source Type: research

Tomato endo beta-mannanase: A candidate of potential tomato allergen protein detected with human monoclonal antibody established from a patient suffered from Japanese cedar pollinosis.
Authors: Endo S, Yamada T, Naganawa Y, Tabuchi T, Shinmoto H Abstract Peripheral blood lymphocytes from a patient allergic to Japanese cedar pollens were transformed by Epstein-Barr virus infection. Some transformed B-lymphoblastoid cells (BLCs) secreted IgM class antibodies to cedar pollen extracts and tomato fruit extracts. One stable human-mouse hybridoma clone Y-22-3-3 secreting IgM class monoclonal antibody to tomato fruit extracts was established by cell fusion of BLCs with mouse myeloma cells. Western blot analysis of tomato extracts showed Y-22-3-3 monoclonal antibody recognized a tomato protein with a mole...
Source: Human Antibodies - January 1, 2019 Category: Biochemistry Tags: Hum Antibodies Source Type: research

Analysis of STAT1, STAT2 and STAT3 mRNA expression levels in the blood of patients with multiple sclerosis.
This study aimed to assess STAT1, STAT2 and STAT3 gene expression in the blood of 50 relapsing-remitting MS (RR-MS) patients and 50 healthy controls by TaqMan Quantitative Real-Time PCR. RESULTS: The results showed that STAT1 gene expression was significantly up-regulated (p= 0.023), whereas STAT2 gene expression was significantly down-regulated (p
Source: Human Antibodies - November 11, 2018 Category: Biochemistry Tags: Hum Antibodies Source Type: research

Immunogenicity comparison of lactoferrin purified from Saudi Arabia camel clans milk.
Authors: Almehdar HA, El-Baky NA, Alhaider AA, Redwan EM Abstract Secretory lactoferrins play a crucial rolls at mucosal surfaces as not only antimicrobial molecules in primate as well as human, but as physiological protein. Its multiple functions extended to be one of immunogen could elicited autoimmune disorders. Purified camel lactoferrin (cLfs) from different Saudi camel clans were shown to be a potent immunogen when injected into rabbit. Four rabbit were subcutaneously immunized with different camel clans lactoferrin/Freunds adjuvant. Anti-cLfs potency titration was reach 1:32000 and did not significantly diff...
Source: Human Antibodies - October 31, 2018 Category: Biochemistry Tags: Hum Antibodies Source Type: research

Production and characterization of monoclonal antibodies against recombinant extracellular domain of CD99.
CONCLUSION: Based on these results, we propose that the purified monoclonal antibody 547F2 4F12 could be possibly used for targeting tumors which over express CD99. PMID: 30282350 [PubMed - as supplied by publisher] (Source: Human Antibodies)
Source: Human Antibodies - October 6, 2018 Category: Biochemistry Tags: Hum Antibodies Source Type: research