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Formulation of glutathione responsive anti-proliferative nanoparticles from thiolated Akt1 siRNA and disulfide-crosslinked PEI for efficient anti-cancer gene therapy
In this study, thiol-modified siRNA (SH-siRNA) was delivered by bioreducible polyethylenimine (ssPEI), to enhance physicochemical properties of polyplexes and function of siRNA through disulfide bonding between SH-siRNA and ssPEI. The ssPEI was utilized to deliver Akt1 SH-siRNA for suppression of Akt1 mRNA and blockage of Akt1 protein translation, resulting in reduced cellular proliferation and the induction of apoptosis. Disulfide bondings between the ssPEI and SH-siRNA through thiol groups in both were confirmed by DTT treatment. Complexation between ssPEI and Akt1SH-siRNA was enhanced and reduced surface charge of ssPEI...
Source: Colloids and Surfaces B: Biointerfaces - January 9, 2015 Category: Biochemistry Source Type: research
Antibody-mediated delivery of anti-KRAS-siRNA in vivo overcomes therapy resistance in colon cancer.
Conclusions:The coupling of siRNA against KRAS to anti-EGFR antibodies provides a novel therapy approach for KRAS-mutated EGFR-positive cancer cells in vitro and in vivo. These findings provide an innovative approach for cancer specific siRNA application and for enhanced therapeutic potential of monoclonal antibody therapy and personalized treatment of cancer entities.
PMID: 25589625 [PubMed - as supplied by publisher]
Source: Clinical Cancer Research - January 14, 2015 Category: Cancer & Oncology Authors: Baeumer S, Baeumer N, Appel N, Terheyden L, Fremerey J, Schelhaas S, Wardelmann E, Buchholz F, Berdel WE, Müller-Tidow C Tags: Clin Cancer Res Source Type: research
Peptide‐like Polymers Exerting Effective Glioma‐Targeted siRNA Delivery and Release for Therapeutic Application
Lipopolymer 49, a solid‐phase synthesized T‐shaped peptide‐like oligoamide containing two central oleic acids, 20 aminoethane, and two terminal cysteine units, is identified as very potent and biocompatible small interfering RNA (siRNA) carrier for gene silencing in glioma cells. This carrier is combined with a novel targeting polymer 727, containing a precise sequence of Angiopep 2 targeting peptide, linked with 28 monomer units of ethylene glycol, 40 aminoethane, and two terminal cysteines in siRNA complex formation. Angiopep‐polyethylene glycol (PEG)/siRNA polyplexes exhibit good nanoparticle features, effective...
Source: Small - July 1, 2015 Category: Nanotechnology Authors: Sai An, Dongsheng He, Ernst Wagner, Chen Jiang Tags: Full Paper Source Type: research
IL-1β siRNA adenovirus benefits liver regeneration by improving mesenchymal stem cells survival after acute liver failure.
CONCLUSION: IL-1? siRNA adenovirus could enhance MSC ability of tissue regeneration through increasing its survival rate. Accordingly, combination of IL-1? siRNA adenovirus and MSC had a synergistic effect on acute liver failure.
PMID: 26845604 [PubMed - as supplied by publisher]
Source: Annals of Hepatology - February 5, 2016 Category: Gastroenterology Tags: Ann Hepatol Source Type: research
Development of a cell-defined siRNA microarray for analysis of gene functionin human bone marrow stromal cells
Publication date: March 2016 Source:Stem Cell Research, Volume 16, Issue 2 Author(s): H.C. Kim, G.-H. Kim, S.-G. Cho, E.J. Lee, Y.-J. Kwon Small interfering RNA (siRNA) screening approaches have provided useful tools for the validation of genetic functions; however, image-based siRNA screening using multiwell plates requires large numbers of cells and time, which could be the barrier in application for gene mechanisms study using human adult cells. Therefore, we developed the advanced method with the cell-defined siRNA microarray (CDSM), for functional analysis of genes in small scale within slide glass using human ...
Source: Stem Cell Research - February 20, 2016 Category: Stem Cells Source Type: research
Binary‐copolymer system base on low‐density lipoprotein‐coupled N‐succinyl chitosan lipoic acid micelles for co‐delivery MDR1 siRNA and paclitaxel, enhances antitumor effects via reducing drug
Abstract
The development of effective and stable carriers of small interfering RNA (siRNA) is important for treating cancer with multidrug resistance (MDR). We developed a new gene and drug co‐delivery system and checked its characteristics. Low‐density lipoprotein (LDL) was coupled with N‐succinyl chitosan (NSC) Lipoic acid (LA) micelles and co‐delivered MDR1 siRNA and paclitaxel (PTX‐siRNA/LDL‐NSC‐LA) to enhance antitumor effects by silencing the MDR gene of tumors (Li et al., Adv Mater 2014;26:8217‐8224). In our study, we developed a new type of containing paclitaxel‐loaded micelles and siRNA‐loaded ...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - March 23, 2016 Category: Materials Science Authors: Shu‐Di Yang, Wen‐Jing Zhu, Qiao‐Ling Zhu, Wei‐Liang Chen, Zhao‐Xiang Ren, Fang Li, Zhi‐Qiang Yuan, Ji‐Zhao Li, Yang Liu, Xiao‐Feng Zhou, Chun Liu, Xue‐Nong Zhang Tags: Original Research Report Source Type: research
Development of a cell-defined siRNA microarray for analysis of gene function in human bone marrow stromal cells
Publication date: March 2016 Source:Stem Cell Research, Volume 16, Issue 2 Author(s): Hi Chul Kim, Gi-Hwan Kim, Ssang-Goo Cho, Eun Ju Lee, Yong-Jun Kwon Small interfering RNA (siRNA) screening approaches have provided useful tools for the validation of genetic functions; however, image-based siRNA screening using multiwell plates requires large numbers of cells and time, which could be the barrier in application for gene mechanisms study using human adult cells. Therefore, we developed the advanced method with the cell-defined siRNA microarray (CDSM), for functional analysis of genes in small scale within slide glas...
Source: Stem Cell Research - April 5, 2016 Category: Stem Cells Source Type: research
Transcutaneous iontophoretic delivery of STAT3 siRNA using layer-by-layer chitosan coated gold nanoparticles to treat melanoma
In conclusion, layer-by-layer chitosan coated AuNP can be developed as a carrier for iontophoretic delivery of STAT3 siRNA to treat melanoma. Graphical abstract
Source: Colloids and Surfaces B: Biointerfaces - June 16, 2016 Category: Biochemistry Source Type: research
Knockdown of β-catenin by siRNA influences proliferation, apoptosis and invasion of the colon cancer cell line SW480.
Authors: Li K, Zhou ZY, Ji PP, Luo HS
Abstract
The aim of the present study was to explore the effect of knocking down the expression of β-catenin by small interference (si)RNA on the activity of the Wnt/β-catenin signaling pathway, and the proliferation, apoptosis and invasion abilities of the human colon cancer cell line SW480. For that purpose, double-stranded siRNA targeting β-catenin (β-catenin-siRNA) was synthesized and transfected into SW480 cells. Reverse transcription-polymerase chain reaction (RT-PCR) and western blotting were used to detect the messenger (m)RNA and protein levels of β-catenin in SW4...
Source: Oncology Letters - June 19, 2016 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research
SiRNA/DOX lodeded chitosan based nanoparticles: Development, Characterization and in vitro evaluation on A549 lung cancer cell line.
Authors: Seifi-Najmi M, Hajivalili M, Safaralizadeh R, Sadreddini S, Esmaeili S, Razavi R, Ahmadi M, Mikaeili H, Baradaran B, Shams-Asenjan K, Yousefi M
Abstract
High-mobility group AT-hook2 (HMGA2), involved in epithelial mesenchymal transition (EMT) process, has a pivotal role in lung cancer metastasis. Lung cancer therapy with HMGA2 suppressing small interfering RNA (siRNA) has been introduced recently while doxorubicin (DOX) has been used as a frequent cancer chemotherapy agent. Both reagents have been faced with obstacles in clinic which make them ineffective. NanoParticles (NPs) provided a platform for effici...
Source: Cellular and Molecular Biology - October 20, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research
Abstract P6-08-01: A directed siRNA screen identifies INHBA as a major regulator of tumor aggressiveness in basal HER2 breast cancer
In conclusion, we identified INHBA as a major regulator of metabolism and aggressiveness in HER2+ basal breast cancer cells that is associated with poor outcome in patients.Citation Format: Korkola JE, Liu M, Smith R, Liby T, Heiser L, Gray JW. A directed siRNA screen identifies INHBA as a major regulator of tumor aggressiveness in basal HER2 breast cancer [abstract]. In: Proceedings of the Thirty-Ninth Annual CTRC-AACR San Antonio Breast Cancer Symposium; 2016 Dec 6-10; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2017;77(4 Suppl):Abstract nr P6-08-01.
Source: Cancer Research - February 13, 2017 Category: Cancer & Oncology Authors: JE Korkola, M Liu, R Smith, T Liby, L Heiser, JW Gray Tags: Poster Session Abstracts Source Type: research
Targeting neuronal nitric oxide synthase by a cell penetrating peptide Tat-LK15/siRNA bioconjugate
Publication date: 22 May 2017 Source:Neuroscience Letters, Volume 650 Author(s): Jie Peng, Yun Rao, Xue Yang, Ji Jia, Youping Wu, Jianhua Lu, Yuanxiang Tao, Weifeng Tu We developed a cell penetrating peptide (CPP) Tat-LK15, as a siRNA carrier to target nNOS. The feasibility, stability, efficiency and selectivity of this peptide-siRNA complex were evaluated in rat neuronal cells. We also compared the new method with conventional siRNA carrier Lipofectamine™. It was found that the CPP Tat-LK15 effectively and specifically delivered nNOS-siRNA into Rat retinal ganglia (RGC-5) cells and silenced the expression of nNOS. The ...
Source: Neuroscience Letters - April 30, 2017 Category: Neuroscience Source Type: research
TPP-dendrimer nanocarriers for siRNA delivery to the pulmonary epithelium and their dry powder and metered-dose inhaler formulations
Publication date: 15 July 2017 Source:International Journal of Pharmaceutics, Volume 527, Issues 1–2 Author(s): Elizabeth Bielski, Qian Zhong, Hamad Mirza, Matthew Brown, Ashura Molla, Teresa Carvajal, Sandro R.P. da Rocha The regulation of genes utilizing the RNA interference (RNAi) mechanism via the delivery of synthetic siRNA has great potential in the treatment of a variety of lung diseases. However, the delivery of siRNA to the lungs is challenging due to the poor bioavailability of siRNA when delivered intraveneously, and difficulty in formulating and maintaining the activity of free siRNA when delivered directly ...
Source: International Journal of Pharmaceutics - June 1, 2017 Category: Drugs & Pharmacology Source Type: research
Recent advances in mechanism-based chemotherapy drug-siRNA pairs in co-delivery systems for cancer: A review.
Abstract
Co-delivery of chemotherapy drugs and siRNA for cancer therapy has achieved remarkable results according to synergistic/combined antitumor effects, and is recognized as a promising therapeutic modality. However, little attention has been paid to the extremely complex mechanisms of chemotherapy drug-siRNA pairs during co-delivery process. Proper selection of chemotherapy drug-siRNA pairs is beneficial for achieving desirable cancer therapeutic effects. Exploring the inherent principles during chemotherapy drug-siRNA pair selection for co-delivery would greatly enhanced therapeutic efficiency. To achieve id...
Source: Colloids and Surfaces - June 8, 2017 Category: Biotechnology Authors: Wang M, Wang J, Li B, Meng L, Tian Z Tags: Colloids Surf B Biointerfaces Source Type: research
Binary ‐copolymer system base on low‐density lipoprotein‐coupled N‐succinyl chitosan lipoic acid micelles for co‐delivery MDR1 siRNA and paclitaxel, enhances antitumor effects via reducing drug
Abstract
The development of effective and stable carriers of small interfering RNA (siRNA) is important for treating cancer with multidrug resistance (MDR). We developed a new gene and drug co‐delivery system and checked its characteristics. Low‐density lipoprotein (LDL) was coupled with N‐succinyl chitosan (NSC) Lipoic acid (LA) micelles and co‐delivered MDR1 siRNA and paclitaxel (PTX‐siRNA/LDL‐NSC‐LA) to enhance antitumor effects by silencing the MDR gene of tumors (Li et al., Adv Mater 2014;26:8217‐8224). In our study, we developed a new type of containing paclitaxel‐loaded micelles and siRNA‐loaded ...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - March 23, 2016 Category: Materials Science Authors: Shu ‐Di Yang, Wen‐Jing Zhu, Qiao‐Ling Zhu, Wei‐Liang Chen, Zhao‐Xiang Ren, Fang Li, Zhi‐Qiang Yuan, Ji‐Zhao Li, Yang Liu, Xiao‐Feng Zhou, Chun Liu, Xue‐Nong Zhang Tags: Original Research Report Source Type: research
