In vitro and in vivo osteogenic potential of niobium ‐doped 45S5 bioactive glass: A comparative study
AbstractIn vitro and in vivo experiments were undertaken to evaluate the solubility, apatite ‐forming ability, cytocompatibility, osteostimulation, and osteoinduction for a series of Nb‐containing bioactive glass (BGNb) derived from composition of 45S5 Bioglass. Inductively coupled plasma optical emission spectrometry (ICP‐OES) revealed that the rate at which Na, Ca, Si, P, and Nb spe cies are leached from the glass decrease with the increasing concentration of the niobium oxide. The formation of apatite as a function of time in simulated body fluid was monitored by 31P Magic Angle Spinning (MAS) Nuclear magnetic res...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - October 4, 2019 Category: Materials Science Authors: Jo ão H. Lopes, Lucas P. Souza, Juliana A. Domingues, Filipe V. Ferreira, Moema Alencar Hausen, José A. Camilli, Richard A. Martin, Eliana A. Rezende Duek, Italo O. Mazali, Celso A. Bertran Tags: ORIGINAL RESEARCH REPORT Source Type: research

Issue Information
Journal of Biomedical Materials Research Part B: Applied Biomaterials, Volume 107, Issue 8, Page 2451-2456, November 2019. (Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials)
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - October 3, 2019 Category: Materials Science Tags: Issue Information Source Type: research

Collagen and bone morphogenetic protein ‐2 functionalized hydroxyapatite scaffolds induce osteogenic differentiation in human adipose‐derived stem cells
In this study, the surface of porous HAp scaffold was modified by collagen treatment and bone morphogenetic protein ‐2 (BMP‐2) conjugation. The surface modification did not affect the HAp scaffold's bulk properties. No significant difference in compressive strength was found among different scaffolds, with HAp, collagen modified HAp, and collagen–BMP‐2‐functionalized HAp having compressive strengths of 45.8 ± 3.12, 51.2 ± 4.09, and 50.7 ± 3.98 MPa, respectively. In vitro studies were performed to compare adhesion and osteogenic differentiat...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - October 1, 2019 Category: Materials Science Authors: Nguyen T. B. Linh, Celine D. G. Abueva, Dong ‐Woo Jang, Byong‐Taek Lee Tags: ORIGINAL RESEARCH REPORT Source Type: research

A cell ‐free approach with a supporting biomaterial in the form of dispersed microspheres induces hyaline cartilage formation in a rabbit knee model
The objective of this study was to test a regenerative medicine strategy for the regeneration of articular cartilage. This approach combines microfracture of the subchondral bone with the implant at the site of the cartilage defect of a supporting biomaterial in the form of microspheres aimed at creating an adequate biomechanical environment for the differentiation of the mesenchymal stem cells that migrate from the bone marrow. The possible inflammatory response to these biomaterials was previously studied by means of the culture of RAW264.7 macrophages. The microspheres were implanted in a 3  mm‐diameter defect ...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - September 14, 2019 Category: Materials Science Authors: Javier Zurriaga Carda, Maria L. Lastra, Carmen M. Antolinos ‐Turpin, Rosa M. Morales‐Román, María Sancho‐Tello, Sofía Perea‐Ruiz, Lara Milián, Juan M. Fernández, Ana M. Cortizo, Carmen Carda, Gloria Gallego‐Ferrer, José L. Góme Tags: ORIGINAL RESEARCH REPORT Source Type: research

Layer ‐by‐layer films based on catechol‐modified polysaccharides produced by dip‐ and spin‐coating onto different substrates
AbstractLayer ‐by‐layer films based on chitosan and hyaluronic acid were produced by dip‐ and spin‐coating techniques onto glass, 316L stainless steel and titanium. These natural polymers were modified with catechol groups, in order to build coatings with improved adhesive properties. Polymeric coatings w ere exclusively composed by both modified polymers whereas the multifunctional coatings combined an inorganic phase of bioactive glass nanoparticles with the polymeric layers to confer bioactivity. Ultraviolet–visible spectroscopy demonstrated that both polymers were successfully synthesized. Four ier transf...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - September 13, 2019 Category: Materials Science Authors: Ana C. Almeida, Ana C. Vale, Ricardo A. Pires, Rui L. Reis, Nat ália M. Alves Tags: ORIGINAL RESEARCH REPORT Source Type: research

Chitosan ‐laponite nanocomposite scaffolds for wound dressing application
AbstractThe pivotal issue of skin regeneration research is the development of effective biomaterials that exhibit biological activities as fungicide and bactericide, combining simple and low cost manufacturing technologies. In this context, nanocomposite scaffolds based on chitosan (Ch)/Laponite (Lap) were produced by using different concentrations of Lap via freeze ‐drying process for potential application in skin regeneration. The influence of Lap concentration on the scaffold properties was evaluated. The prepared scaffolds were characterized by x‐ray diffraction (XRD), scanning electron microscopy (SEM), porosity, ...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - September 12, 2019 Category: Materials Science Authors: Virg ínia de A. M. Gonzaga, Alessandra L. Poli, Juliana S. Gabriel, Daiane Y. Tezuka, Talita A. Valdes, Andrei Leitão, Camila F. Rodero, Taís M. Bauab, Marlus Chorilli, Carla C. Schmitt Tags: ORIGINAL RESEARCH REPORT Source Type: research

Promotion through external magnetic field of osteogenic differentiation potential in adipose ‐derived mesenchymal stem cells: Design of polyurethane/poly(lactic) acid sponges doped with iron oxide nanoparticles
AbstractRecently, iron oxide nanoparticles (IONPs) have gathered special attention in regenerative medicine. Owing to their magnetic and bioactive properties, IONPs are utilized in the fabrication of novel biomaterials. Yet, there was no report regarding thermoplastic polyurethane (TPU) and poly(lactic acid) (PLA) polymer doped with IONPs on osteogenic differentiation of mesenchymal stem cells. Thus the objectives of presented study was to: (a) fabricate magnetic TPU  + PLA sponges doped with iron (III) oxide Fe2O3 nanoparticles; (b) investigate the effects of biomaterial and its exposition to static magnetic...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - September 12, 2019 Category: Materials Science Authors: Krzysztof Marycz, Michalina Alicka, Katarzyna Kornicka ‐Garbowska, Joanna Polnar, Anna Lis‐Bartos, Rafał J. Wiglusz, Michael Roecken, Jean‐Marie Nedelec Tags: ORIGINAL RESEARCH REPORT Source Type: research

Doxycycline containing hydroxyapatite ceramic microspheres as a bone ‐targeting drug delivery system
AbstractDrug delivery technology is a promising way to enhance the therapeutic efficacy of drugs. The purpose of this study is to evaluate the physical and chemical properties of hydroxyapatite ceramic microspheres loaded with doxycycline (HADOX), their effects on in vitro osteoblast viability, and their antimicrobial activity, and to determine the effects of DOX on the healing of rat sockets after tooth extraction. The internal microsphere porosity was sensitive to the treatment used to adsorb DOX onto microsphere surface; HA microspheres without DOX presented 26% of pores, whereas HADOX0.15 microspheres presented 52.0%. ...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - September 8, 2019 Category: Materials Science Authors: Carlos Soriano ‐Souza, Helder Valiense, Elena Mavropoulos, Victor Martinez‐Zelaya, Andrea Machado Costa, Adriana T. Alves, Mariana Longuinho, Rodrigo Resende, Carlos Mourão, Jose Granjeiro, Maria H. Rocha‐Leao, Alexandre Rossi, Mônica Tags: ORIGINAL RESEARCH REPORT Source Type: research

Issue Information
Journal of Biomedical Materials Research Part B: Applied Biomaterials, Volume 107, Issue 7, Page 2203-2208, October 2019. (Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials)
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - September 3, 2019 Category: Materials Science Tags: Issue Information Source Type: research

Retrieval analysis of TiN (titanium nitride) coated knee replacements: Coating wear and degradation in vivo
ConclusionTiN coatings of knee replacements undergo wear and degradation related to presence of third bodies and microscopic defects on their surface. Since coating integrity may be compromised in vivo, such implants should be used with caution in metal sensitive patients. (Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials)
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - August 31, 2019 Category: Materials Science Authors: Łukasz Łapaj, Justyna Rozwalka Tags: CLINICAL DEVICE ‐RELATED ARTICLE Source Type: research

Bioactive glass ions for in vitro osteogenesis and microvascularization in gellan gum ‐collagen hydrogels
In conclusion, we demonstrated efficient hASC o steogenic differentiation and microvessel‐like network formation in coculture with HUVECs. (Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials)
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - August 31, 2019 Category: Materials Science Authors: Kaisa Vuornos, Heini Huhtala, Minna K ääriäinen, Kirsi Kuismanen, Leena Hupa, Minna Kellomäki, Susanna Miettinen Tags: ORIGINAL RESEARCH REPORT Source Type: research

Bone augmentation using small molecules with biodegradable calcium sulfate particles in a vertical onlay graft model in the rabbit calvarium
AbstractSmall molecules including sodium butyrate (SB) and dimethyloxalylglycine (DMOG) can promote bone regeneration via inhibitive effects eliciting cellular responses through signaling cascades. The purpose of this study was to determine the synergistic effects of SB and DMOG loaded on calcium sulfate (CaS) on bone regeneration in the challenging vertical augmentation model in the rabbit calvarium. Four plastic cylinders screwed on the calvarium of each of 10 rabbits were randomly grafted with CaS, CaS/SB, CaS/DMOG, or CaS/DMOG/SB. All specimens were assessed by radiographic, histologic, and histomorphometric analyses. ...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - August 30, 2019 Category: Materials Science Authors: Doo H. Kim, Jae ‐Kook Cha, Young W. Song, Kyung M. Woo, Ui‐Won Jung Tags: ORIGINAL RESEARCH REPORT Source Type: research

Additively manufactured small ‐diameter vascular grafts with improved tissue healing using a novel SNAP impregnation method
AbstractThe vascular network has a complex architecture such as branches, curvatures, and bifurcations which is even more complicated in view of individual patients' defect anatomy requiring custom ‐specifically designed vascular implants. In this work, 3D printing is used to overcome these challenges and a new shorter impregnation method was developed to incorporate S‐nitroso‐N‐acetyl‐d‐penicillamine (SNAP) as a nitric oxide (NO) donor to printed grafts. The 3D‐printed small‐diameter vascular grafts (SDVGs) were impregnated with SNAP solution during SNAP synthesis (S1) or with SNAP dissolved in methanol (S...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - August 30, 2019 Category: Materials Science Authors: Fatemeh Kabirian, Peiman Brouki Milan, Ali Zamanian, Ruth Heying, Masoud Mozafari Tags: ORIGINAL RESEARCH REPORT Source Type: research

Hot isostatic pressure treatment of 3D printed Ti6Al4V alters surface modifications and cellular response
AbstractAdditive manufacturing can be used to create personalized orthopedic and dental implants with varying geometries and porosities meant to mimic morphological properties of bone. These qualities can alleviate stress shielding and increase osseointegration through bone ingrowth, but at the expense of reduced fatigue properties compared to machined implants, and potential for loose build particle erosion. Hot isostatic pressure (HIP) treatment is used to increase fatigue resistance; implant surface treatments like grit ‐blasting and acid‐etching create microroughness and reduce the presence of loose particles. Howe...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - August 30, 2019 Category: Materials Science Authors: Michael B. Berger, Thomas W. Jacobs, Barbara D. Boyan, Zvi Schwartz Tags: ORIGINAL RESEARCH REPORT Source Type: research

Biocompatibility of (Ba,Ca)(Zr,Ti)O3 piezoelectric ceramics for bone replacement materials
AbstractTotal joint replacement implants are generally designed to physically mimic the biological environment to ensure compatibility with the host tissue. However, implant instability exposes patients to long recovery periods, high risk for revision surgeries, and high expenses. Introducing electrical stimulation to the implant site to accelerate healing is promising, but the cumbersome nature of wired devices is detrimental to the implant design. We propose a novel strategy to stimulate cells at the implant site by utilizing piezoelectric ceramics as electrical stimulation sources. The inherent ability of these material...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - August 24, 2019 Category: Materials Science Authors: Kara K. Poon, Matthias C. Wurm, Donald M. Evans, Mari ‐Ann Einarsrud, Rainer Lutz, Julia Glaum Tags: ORIGINAL RESEARCH REPORT Source Type: research

Delivery of long chain C16 and C24 ceramide in HeLa cells using oxidized graphene nanoribbons
AbstractThe bioactive sphingolipid ceramide has many important roles in cell signaling processes, particularly in signaling programmed cell death in cancer. However, ceramide levels are often impaired in multi ‐drug resistant and radiation resistant cancers due to the dysregulation of ceramide metabolism. Restoration of ceramide levels through external delivery therefore represents a potential therapeutic target for the treatment of resistant cancers. However, as a lipid, ceramide is extremely hydrophob ic and requires a delivery system to enter cells. Here we report the development of a method to load significant amount...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - August 24, 2019 Category: Materials Science Authors: Cassandra Suhrland, Jean ‐Philip Truman, Lina M. Obeid, Balaji Sitharaman Tags: ORIGINAL RESEARCH REPORT Source Type: research

Inhibition of melanin synthesis and melanosome transfer by chitosan biomaterials
In this study, we investigated the effects of chitosan, a natural marine product, on melanin synthesis and melanosome transfer. Treating B16F10 melanoma cells caused the inhibitory effect of chitosan on melanogenesis to be more prominent under α‐melanocyte‐stimulating hormone (α‐MSH) stimulation. Chitosan samples of different molecular weights inhibited melanogenesis to a comparable extent, whereas increasing the deacetylation of chitosan enhanced its depigmentation effects. Chitosan was found to effectively reduce basal or α‐M SH‐stimulated melanogenesis by suppressing the expression of melanog...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - August 22, 2019 Category: Materials Science Authors: Hsi ‐Wen Chen, Ya‐Shuan Chou, Tai‐Horng Young, Nai‐Chen Cheng Tags: ORIGINAL RESEARCH REPORT Source Type: research

Three ‐dimensional electrospun nanofibrous scaffolds for bone tissue engineering
AbstractElectrospinning technology has been widely used in the past few decades to prepare nanofibrous scaffolds that mimic extracellular matrices. However, traditional two ‐dimensional (2D) electrospun nanofibrous mats still have some inherent disadvantages for bone tissue engineering, such as limited cell infiltration and lack of three‐dimensional (3D) structure. The development of 3D electrospun scaffolds with larger pore sizes and porosity provides new perspect ives for electrospinning‐based tissue engineering scaffolds. However, there are still some challenges and areas for improvement. In this review, the appli...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - August 22, 2019 Category: Materials Science Authors: Weimin Lin, Miao Chen, Tao Qu, Jidong Li, Yi Man Tags: REVIEW ARTICLE Source Type: research

A new approach to the production of a biovital skin graft based on human acellular dermal matrix produced in ‐house, in vitro revitalized internally by human fibroblasts and keratinocytes on the surface
AbstractPatients with extensive and deep burns who do not have enough donor sites for autologous skin grafts require alternative treatment methods. Tissue engineering is a useful tool to solve this problem. The aim of this study was to find the optimal method for the production of a biovital skin substitute based on acellular dermal matrix (ADM) and in vitro cultured fibroblasts and keratinocytes. In this work, nine methods of ADM production were assessed. The proposed methods are based on the use of the following enzymes: Dispase II, collagenase I/ethylenediaminetetraacetic acid (EDTA), collagenase II/EDTA, and mechanical...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - August 20, 2019 Category: Materials Science Authors: Wojciech Łabuś, Diana Kitala, Agnieszka Klama‐Baryła, Michał Szapski, Wojciech Smętek, Małgorzata Kraut, Ryszard Poloczek, Justyna Glik, Anna Pielesz, Dorota Biniaś, Ewa Sarna, Joanna Grzybowska‐Pietras, Marek Kucharzewski Tags: ORIGINAL RESEARCH REPORT Source Type: research

The effect of platelet ‐rich fibrin exudate addition to porous poly(lactic‐co‐glycolic acid) scaffold in bone healing: An in vivo study
In this study a novel procedure was adopted to incorporate both the dissolved contents and mechanical components of leukocyte‐ and platelet‐rich fibrin (L‐PRF) into an PLGA scaffold through a two‐step method: (a) extraction of the L‐PRF membrane transudate with subsequent immersion of the PLGA scaffold in transudate followed by (b) delivering a fibrin gel as a low‐viscosity component that subsequently polymerizes into a highly viscous, gel‐like biological material within the pores of the PLGA scaffold. Two, ~0.40 cm3, submandibular defects (n =  24) were created per side using rotary instrumenta...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - August 19, 2019 Category: Materials Science Authors: Lukasz Witek, Han Tian, Nick Tovar, Andrea Torroni, Rodrigo Neiva, Luiz F. Gil, Paulo G. Coelho Tags: ORIGINAL RESEARCH REPORT Source Type: research

Reviewing recently developed technologies to direct cell activity through the control of pore size: From the macro ‐ to the nanoscale
AbstractScaffold pore size plays a fundamental role in the regeneration of new tissue since it has been shown to direct cell activity in situ. It is well known that cellular response changes in relation with pores diameter. Consequently, researchers developed efficient approaches to realize scaffolds with controllable macro ‐, micro‐, and nanoporous architecture. In this context, new strategies aiming at the manufacturing of scaffolds with multiscale pore networks have emerged, in the attempt to mimic the complex hierarchical structures found in living systems. In this review, we aim at providing an overview of the fab...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - August 19, 2019 Category: Materials Science Authors: Viola Sgarminato, Chiara Tonda ‐Turo, Gianluca Ciardelli Tags: REVIEW ARTICLE Source Type: research

Improved exposure of bioactive peptides to the outermost surface of the polylactic acid nanofiber scaffold
In this study, we evaluated two strategies to improve the exposing efficiency of IKVAV. One strategy introduced a hydrophilic diethy lene glycol (diEG) segment between IKVAV and ODLA. In the second method, these modified nanofibers were heated to a given temperature and cooled to room temperature in water. The IKVAV peptides at the outermost surface were quantified by the fluorescein isothiocyanate staining method. After being he ated above the PLLA glass transition temperature, the exposing efficiency of the IKVAV sequence on the PLLA/ODLA–diEG–IKVAV nanofiber was three times higher than that on the PLLA/ODLA&...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - August 19, 2019 Category: Materials Science Authors: Yu ‐I Hsu, Tetsuji Yamaoka Tags: ORIGINAL RESEARCH REPORT Source Type: research

CoCrMo metal release in metal ‐on‐highly crosslinked polyethylene hip implants
This study provides strong evidence that tribocorrosion at the articulating surfaces in MoP hip implants could result in significant metal release. This inf ormation should be taken into account when studying the mechanisms of ALTRs and developing strategies of preventing metal release in total hip replacements. (Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials)
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - August 19, 2019 Category: Materials Science Authors: Qiong Wang, Felipe Eltit, Donald Garbuz, Clive Duncan, Bassam Masri, Nelson Greidanus, Rizhi Wang Tags: ORIGINAL RESEARCH REPORT Source Type: research

Implementation of a dynamic culture condition to the heterotypic 3D breast cancer model
AbstractCell culture system is used for a wide range of research and biotechnology production. Majority of in vitro cell studies are conducted as static, two dimensional (2D) dish culture system where cells grow in a monolayer. However, to better reflect the in vivo condition, three dimensional (3D) culture systems were introduced that allow investigating the cell –cell and cell–microenvironment interactions. In this work, the 3D breast cancer model was investigated. Previously, we developed a 3D breast cancer model that constituted of fibroblasts and breast cancer cells seeded on the silkworm silk scaffold. Th...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - August 16, 2019 Category: Materials Science Authors: Karolina Penderecka, Matthew Ibbs, Apolonia Kaluzna, Anna Lewandowska, Andrzej Marszalek, Andrzej Mackiewicz, Hanna Dams ‐Kozlowska Tags: Original Research Report Source Type: research

In vivo hydroxyapatite scaffold performance in infected bone defects
This study proposes an alternative to the autograft using a porous, hydroxyapatite (HA) scaffold evaluated with and without infection and antibiotics. Twenty ‐four New Zealand white rabbits received either our HA scaffold or a pulverized autograft (PBA) within a surgically created critical‐sized defect in the femur. The two grafts were evaluated in either septic or aseptic defects and with or without antibiotic treatment. The HA scaffolds were charac terized with micro computed tomography. Post‐euthanasia, micro computed tomography, histology, and white blood cells component analysis were completed. The HA had significantly greater (p
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - August 13, 2019 Category: Materials Science Authors: Joseph J. Pearson, Nicholas Gerken, Chunsik Bae, Kyu ‐Bok Lee, Arpan Satsangi, Sean McBride, Mark R. Appleford, David D. Dean, Jeffrey O. Hollinger, Joo L. Ong, Teja Guda Tags: ORIGINAL RESEARCH REPORT Source Type: research

A bench ‐top molding method for the production of cell‐laden fibrin micro‐fibers with longitudinal topography
AbstractTissue ‐engineered constructs have great potential in many intervention strategies. In order for these constructs to function optimally, they should ideally mimic the cellular alignment and orientation found in the tissues to be treated. Here we present a simple and reproducible method for the production of cell‐laden pure fibrin micro‐fibers with longitudinal topography. The micro‐fibers were produced using a molding technique and longitudinal topography was induced by a single initial stretch. Using this method, fibers up to 1 m in length and with diameters of 0.2–3 mm could be produc ...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - August 13, 2019 Category: Materials Science Authors: Hans Keijdener, Jens Konrad, Bernd Hoffmann, Jos é Gerardo‐Nava, Stephan Rütten, Rudolf Merkel, Jaime Vázquez‐Jiménez, Gary A. Brook, Stefan Jockenhoevel, Petra Mela Tags: ORIGINAL RESEARCH REPORT Source Type: research

Effect of ionizing radiation on mechanical properties and translucency of monolithic zirconia
In conclusion, RT may affect flexural strength and crystalline content of zirconia‐based materials, but this effect was not observed under fatigue. Translucency of Y‐PSZ restorations is also not affected by RT. (Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials)
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - August 13, 2019 Category: Materials Science Authors: Abdullah A. Alshamrani, Grace M. De Souza Tags: ORIGINAL RESEARCH REPORT Source Type: research

A biphasic calcium phosphate ceramic scaffold loaded with oxidized cellulose nanofiber –gelatin hydrogel with immobilized simvastatin drug for osteogenic differentiation
AbstractA novel bone scaffold containing bioceramic and biopolymer materials with an osteoinductive simvastatin molecule was developed to enhance bone regeneration. An oxidized cellulose nanofiber (OCNF) –Gelatin (Gel) hydrogel was loaded into a biphasic calcium phosphate (BCP) ceramic in which simvastatin was entrapped, resulting in a scaffold with both osteoconductive and osteoinductive properties. The fabricated scaffold showed interconnected porosity with micro‐ and macroporous orientation. After loading the OCNF–Gel (HG), the mechanical stability of the ceramic BCP scaffold was increased suitable for the...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - August 13, 2019 Category: Materials Science Authors: Omar Faruq, Shithima Sayed, Boram Kim, Soo ‐Bin Im, Byong‐Taek Lee Tags: ORIGINAL RESEARCH REPORT Source Type: research

Histopathological patterns seen around failed metal ‐on‐metal hip replacements: Cluster and latent class analysis of patterns of failure
AbstractWe aimed to establish latent subtypes of histopathological patterns in failed metal ‐on‐metal hip replacements. Tissue samples of the synovia from the neocapsule were retrieved from 284 revised ASR (Articular Surface Replacement) hip replacements and analyzed histologically. Hierarchical cluster analysis and polytomous latent class analysis were performed to establish the under lying structure and relationships of the histological observations and to find similar cohorts of cases. Clustering analyses suggested four distinct subtypes that could be readily and reasonably labeled and mapped against a recent consen...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - August 13, 2019 Category: Materials Science Authors: Aleksi Reito, Lari Lehtovirta, Jyrki Parkkinen, Antti Eskelinen Tags: ORIGINAL RESEARCH REPORT Source Type: research

Evaluation of topographical and chemical modified TiAl6V4 implant surfaces in a weight ‐bearing intramedullary femur model in rabbit
In this study, the surface of TiAl6V4 (Tilastan ®) implants was modified by (a) corundum blasting, (b) corundum blasting followed by electrochemical calcium phosphate (CaP) deposition, and (c) titanium plasma spraying followed by electrochemical CaP deposition. All modifications resulted in a surface roughness suitable to enhance primary implant stabilization and to favor osteoblast adhesion and function; the thin, biomimetic CaP coating is characterized by fast resorbability and served as chemical cue to stimulate osteogenesis. After implantation in a full weight‐bearing rabbit intramedullary distal femur model, oss...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - August 12, 2019 Category: Materials Science Authors: Henriette Bretschneider, Jan Mettelsiefen, Claudia Rentsch, Michael Gelinsky, Helmut D. Link, Klaus ‐Peter Günther, Anja Lode, Christine Hofbauer Tags: ORIGINAL RESEARCH REPORT Source Type: research

A minimally ‐invasive cryogel based approach for the development of human ectopic liver in a mouse model
In this study, we have established an ectopic functional human liver tissue in a mouse model, using a minimally ‐invasive method. Firstly, a human liver tissue mass using HepG2 cells and poly(N‐isopropylacrylamide) (PNIPAAm) incorporated poly(ethylene glycol)‐alginate‐gelatin (PAG) cryogel matrix was developed in vitro. It was later implanted in mouse peritoneal cavity using a 16 G needle. Viscoelastic nature along with low Young's modulus provided injectable properties to the cry ogel. We confirmed minimal cell loss/death while injecting. Further, by in vivo study efficacy of both injectable and surgical im...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - August 9, 2019 Category: Materials Science Authors: Jyoti Kumari, Arun K. Teotia, Anjali A. Karande, Ashok Kumar Tags: ORIGINAL RESEARCH REPORT Source Type: research

Bone regeneration response in an experimental long bone defect orthotopically implanted with alginate ‐pullulan‐glass‐ceramic composite scaffolds
AbstractIn the present study, scaffolds based on alginate ‐pullulan‐bioactive glass‐ceramic with 0.5 and 1.5 mol % copper oxide were orthotopically implanted in experimental rat models to assess their ability to heal an induced bone defect. By implying magnetic resonance and imaging scans together with histological evaluation of the processed samples , a progressive healing of bone was observed within 5 weeks. Furthermore, as the regenerative process continued, new bone tissue was formed, enhancing the growth of irregular bone spicules around the scaffolds. A significantly higher amount of new bone was formed ...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - August 9, 2019 Category: Materials Science Authors: Radu A. Popescu, Flaviu A. T ăbăran, Sidonia Bogdan, Alexandru Fărcăṣanu, Robert Purdoiu, Klara Magyari, Adriana Vulpoi, Alexandra Dreancă, Bogdan Sevastre, Simion Simon, Ionel Papuc, Lucian Baia Tags: ORIGINAL RESEARCH REPORT Source Type: research

Bioengineered corneal epithelial cell sheet from mesenchymal stem cells —A functional alternative to limbal stem cells for ocular surface reconstruction
This study aims to use adipose mesenchymal stem cells (ASC) as an alternative autologous cell source for treating bilateral limbal deficiency conditions. ASCs derived from rabbit fat tissue were differentiated into corneal epithelial lineage using limbal explant condition media. Apart from transdifferentiation, ASC sheets were developed to facilitate effective delivery of these cells to the damage site. A thermoresponsive polymerN‐isopropylacrylamide‐co‐glycidylmethacrylate (NGMA) was synthesized and characterized to demonstrate ASC sheet formation. Transdifferentiated ASCs showed positive expression of corneal epith...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - August 9, 2019 Category: Materials Science Authors: Balu Venugopal, Sachin J. Shenoy, Sumitha Mohan, P. R. Anil Kumar, T. V. Kumary Tags: ORIGINAL RESEARCH REPORT Source Type: research

Oxygen ‐releasing polycaprolactone/calcium peroxide composite microspheres
AbstractOxygen ‐releasing polycaprolactone/calcium peroxide (PCL/CaO2) composite microspheres were fabricated via homogenization, electrospray with a single nozzle, and electrospray with a co ‐axial nozzle, resulting in homogenized, single‐walled, and double‐walled microspheres, respectively. Scanning electron microscopy revealed that homogenized microspheres had pores, while electrosprayed microspheres did not. Alizarin Red S staining showed a core‐shell structure for double‐w alled microspheres. In a hypoxia incubator, single‐walled, double‐walled, and homogenized microspheres could maintain oxygen tensio...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - August 8, 2019 Category: Materials Science Authors: Mengen Zhang, Tawan Kiratiwongwan, Wei Shen Tags: ORIGINAL RESEARCH REPORT Source Type: research

Synthesis and in vivo evaluation of a scaffold containing wollastonite/ β‐TCP for bone repair in a rabbit tibial defect model
AbstractScaffolds are models designed to aid the interaction between cells and extracellular bone matrix, providing structural support for newly formed bone tissue. In this work, wollastonite with β‐TCP porous ceramic scaffolds was developed by the polymer sponge replication. Their microstructure, cell viability and bioactivity were tested. in vivo was performed to evaluate the use of a calcium silicate‐based implant in the repair of rabbit tibias. Holes were made in the both proximal an d distal tibial metaphysis of each animal and filled with calcium silicate‐based implant, and in the left tibia, no implant we...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - August 8, 2019 Category: Materials Science Authors: Willams T. Barbosa, Katilayne V. de Almeida, Gabriel G. de Lima, Miguel A. Rodriguez, Marcos V. Lia Fook, Raul Garc ía‐Carrodeguas, Valdemiro Amaro da Silva Junior, Francisco A. de Sousa Segundo, Marcelo J. C. de Sá Tags: ORIGINAL RESEARCH REPORT Source Type: research

Fabrication and biological activity of polycaprolactone/phlorotannin endotracheal tube to prevent tracheal stenosis: An in vitro and in vivo study
This study demonstrates that an endotracheal tube coated with phlorotannin, which is present in extracts of the brown algaEcklonia cava, can prevent tracheal stenosis in a rabbit model. An in vitro study shows that phlorotannin inhibits proliferation of human tracheal fibroblasts treated with transforming growth factor β1. Phlorotannin‐coated endotracheal tubes show steady release of phlorotannin for up to 7 days, and removal of the tube 1 week after insertion reveals a reduction in both fibrogenesis and thickening of tracheal submucosa. Western blot analysis of tracheal tissues after removal of the phlorotanni n‐...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - August 7, 2019 Category: Materials Science Authors: Hyoung Shin Lee, Min ‐Seon Jeong, Seok‐Chun Ko, Seong‐Yeong Heo, Hyun Wook Kang, Sung Won Kim, Chi Woo Hwang, Kang Dae Lee, Chulho Oak, Min Jung Jung, Junghwan Oh, Won Sun Park, Il‐Whan Choi, Won‐Kyo Jung Tags: ORIGINAL RESEARCH REPORT Source Type: research

Fabrication and biological activity of polycaproalctone/phlorotannin endotracheal tube to prevent tracheal stenosis: An in vitro and in vivo study
This study demonstrates that an endotracheal tube coated with phlorotannin, which is present in extracts of the brown algaEcklonia cava, can prevent tracheal stenosis in a rabbit model. An in vitro study shows that phlorotannin inhibits proliferation of human tracheal fibroblasts treated with transforming growth factor β1. Phlorotannin‐coated endotracheal tubes show steady release of phlorotannin for up to 7 days, and removal of the tube 1 week after insertion reveals a reduction in both fibrogenesis and thickening of tracheal submucosa. Western blot analysis of tracheal tissues after removal of the phlorotanni n‐...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - August 7, 2019 Category: Materials Science Authors: Hyoung Shin Lee, Min Sun Jung, Seok ‐Chun Ko, Seong‐Yeong Heo, Hyun Wook Kang, Sung Won Kim, Chi Woo Hwang, Kang Dae Lee, Chulho Oak, Min Jung Jung, Junghwan Oh, Won Sun Park, Il‐Whan Choi, Won‐Kyo Jung Tags: ORIGINAL RESEARCH REPORT Source Type: research

Ultrasound ‐triggered breast tumor sonodynamic therapy through hematoporphyrin monomethyl ether‐loaded liposome
In this study, we prepared the hematoporphyrin monomethyl ether (H MME)‐loaded liposome as the sonosensitizers for SDT and evaluated their effects on human MCF‐7 breast cancer cellsin vitro andin vivo. Liposomes prepared by thin film hydration technique were about 100  nm in size with positive zeta potential and exhibited spherical in shape. Following irradiation of ultrasound which generates intracellular ROS, the liposome facilitated the delivery of HMME to tumor cells. HMME‐loaded liposomes showed low cytotoxicity under basal condition but significant sono dynamic effects under ultrasonic irradiation. Notab...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - August 6, 2019 Category: Materials Science Authors: Yi Zhang, Yulong Ou, Jia Guo, Xiaojia Huang Tags: ORIGINAL RESEARCH REPORT Source Type: research

Controlled cross ‐linking of porcine cholecyst extracellular matrix for preparing tissue engineering scaffold
AbstractTreatment with cross ‐linking agents for stabilizing biomolecules is an integral step during the preparation of many extracellular matrix‐based tissue engineering scaffolds from mammalian organs. However, excess cross‐linking may cause nonavailability of biomolecules and consequent deterioration of bioinductive pr operties of the scaffold. The present study considered controlling the extent of cross‐linking in a porcine cholecyst extracellular matrix scaffold prepared by a nonenzymatic and nondetergent method, byex situ incubation of the source organ in varying concentrations of neutral buffered formaldehyd...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - August 6, 2019 Category: Materials Science Authors: Manjula P. Mony, Thapasimuthu V. Anilkumar Tags: ORIGINAL RESEARCH REPORT Source Type: research

Grayscale mask ‐assisted photochemical crosslinking for a dense collagen construct with stiffness gradient
This study proposes a novel fabrication process to achieve a compressed collagen con struct with a stiffness gradient, named COSDIENT, at a level of ~ 1 MPa while maintaining in vivo ECM‐like dense collagen fibrillar structures. The COSDIENT was fabricated by collagen compression followed by grayscale mask‐assisted UV–riboflavin crosslinking. The collagen compression process enabled the remarkable increase in the stiffness of the collagen gel from ~ 1–10 kPa to ~ 1 MPa by physical compaction. The subsequent UV–riboflavin crosslinking with a continuous‐tone grayscale mask could simply generate...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - August 6, 2019 Category: Materials Science Authors: Hyeonjun Hong, Sang Min Park, Dohui Kim, Sung Jea Park, Dong Sung Kim Tags: ORIGINAL RESEARCH REPORT Source Type: research

A novel “turn‐on” fluorescent sensor for hydrogen peroxide based on oxidized porous g‐C3N4 nanosheets
In this study, for the first time, a porous fluorescence probe based on CNNs and FeTMPyP was designed and an excellent H2O2 detection performance with a large detection range of 0.1 ~  100 μM and a detection limit of 0.07 μM was achieved. Furthermore, the proposed method was successfully used for H2O2 detection in RAW 264.7 cells. (Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials)
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - August 6, 2019 Category: Materials Science Authors: Zhaoliang Guo, Qiannan Zhao, Yuqian Zhang, Bingdong Li, Lijuan Li, Liwei Feng, Manman Wang, Xianguang Meng, Guifu Zuo Tags: ORIGINAL RESEARCH REPORT Source Type: research

Bioactive glass and high ‐intensity lasers as a promising treatment for dentin hypersensitivity: An in vitro study
AbstractThis in vitro study aimed to analyze the physical and chemical characteristics of the hypersensitive human dentin ‐like surface after application of a bioactive glass (BG) paste (BG/Ac) irradiated or not with high‐power lasers. Dentin specimens were treated with 17% Ethylenediamine tetraacetic acid (EDTA) solution to mimic a hypersensitive dentin and then submitted to neodymium: yttrium–aluminum–garnet (Nd:YAG) laser or CO2 laser irradiation prior and after application of BG/Ac. Characterizations were performed by using X ‐ray diffraction, Fourier transformed infrared spectroscopy, scanning electr...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - August 5, 2019 Category: Materials Science Authors: Ester M. R. Lee, Roger Borges, Juliana Marchi, Carlos Paula Eduardo, M árcia M. Marques Tags: ORIGINAL RESEARCH REPORT Source Type: research

Urethroplasty with a bilayered poly ‐D,L‐lactide‐co‐ε‐caprolactone scaffold seeded with allogenic mesenchymal stem cells
AbstractReconstructive surgery for urethral defects employing tissue ‐engineered scaffolds represents an alternative treatment for urethroplasty. The aim of this study was to compare the therapeutic efficacy of the bilayer poly‐D,L‐lactide/poly‐ε‐caprolactone (PL‐PC) scaffold seeded with allogenic mesenchymal stem cells (MSCs) for urethra reconstruction in a rabbit model with conventional urethroplasty employing an autologous buccal mucosa graft (BG). The inner layer of the scaffold based on poly‐D,L‐lactic acid (PL) was seeded with MSCs, while the outer layer, prepared from poly‐ε‐capro...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - August 1, 2019 Category: Materials Science Authors: Natalia M. Yudintceva, Yulia A. Nashchekina, Nataliya A. Mikhailova, Tatiana I. Vinogradova, Petr K. Yablonsky, Anna A. Gorelova, Alexandr N. Muraviov, Andrey V. Gorelov, Igor A. Samusenko, Boris P. Nikolaev, Ludmila Y. Yakovleva, Maxim A. Shev Tags: ORIGINAL RESEARCH REPORT Source Type: research

Glucosamine ‐grafted methacrylated gelatin hydrogels as potential biomaterials for cartilage repair
AbstractGlucosamine (GlcN) has been widely used to reduce joint pain and osteoarthritis progression, but the efficacy of GlcN remains controversial because of the low GlcN concentration reaching the articular cavity. The aim of this study is to provide an effective approach of GlcN delivery to a target site using photocrosslinkable methacrylated gelatin (GelMA) ‐based hydrogels, where GlcN could be gradually released during the degradation of the GelMA hydrogel. Herein, GlcN was acrylated as the acryloyl glucosamine (AGA) and covalently grafted to GelMA, and more than 87.7% of 15% (w/v) GelMA hydrogel was grafted with AG...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - August 1, 2019 Category: Materials Science Authors: Hairui Suo, Liang Li, Chuanxin Zhang, Jun Yin, Kedi Xu, Jingyi Liu, Jianzhong Fu Tags: ORIGINAL RESEARCH REPORT Source Type: research

Hybrid cardiovascular sourced extracellular matrix scaffolds as possible platforms for vascular tissue engineering
AbstractThe aim when designing a scaffold is to provide a supportive microenvironment for the native cells, which is generally achieved by structurally and biochemically imitating the native tissue. Decellularized extracellular matrix (ECM) possesses the mechanical and biochemical cues designed to promote native cell survival. However, when decellularized and reprocessed, the ECM loses its cell supporting mechanical integrity and architecture. Herein, we propose dissolving the ECM into a polymer/solvent solution and electrospinning it into a fibrous sheet, thus harnessing the biochemical cues from the ECM and the mechanica...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - August 1, 2019 Category: Materials Science Authors: James A. Reid, Anthony Callanan Tags: ORIGINAL RESEARCH REPORT Source Type: research

Silver (Ag) doped magnesium phosphate microplatelets as next ‐generation antibacterial orthopedic biomaterials
This article reports for the first time our successful result in the synthesis of antibacterial single ‐phase newberyite (NB, MgHPO4.3H2O), an important magnesium phosphate (MgP) bioceramic. The prime novelty lies in the fact that we explore novel MgPs as next ‐generation orthopedic biomaterials as opposed to conventional calcium phosphates (CaP). While NB has already shown great promise, unlike its competitor struvite (ST, MgNH4PO4.6H2O), NB is not intrinsically antibacterial. Given the havoc created by surgical site infections (SSI) in orthopedics, it would be worthwhile to explore if antibacterial NB can be synthesi...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - July 31, 2019 Category: Materials Science Authors: Prabaha Sikder, Sarit B. Bhaduri, Joo L. Ong, Teja Guda Tags: ORIGINAL RESEARCH REPORT Source Type: research

System for application of controlled forces on dental implants in rat maxillae: Influence of the number of load cycles on bone healing
AbstractExperimental studies on the effect of micromotion on bone healing around implants are frequently conducted in long bones. In order to more closely reflect the anatomical and clinical environments around dental implants, and eventually be able to experimentally address load ‐management issues, we have developed a system that allows initial stabilization, protection from external forces, and controlled axial loading of implants. Screw‐shaped implants were placed on the edentulous ridge in rat maxillae. Three loading regimens were applied to validate the system;case A no loading (unloaded implant) for 14  d...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - July 31, 2019 Category: Materials Science Authors: Renan Barros e Lima Bueno, Ana P. Dias, Katia J. Ponce, John B. Brunski, Antonio Nanci Tags: ORIGINAL RESEARCH REPORT Source Type: research

High ‐charge‐capacity sputtered iridium oxide neural stimulation electrodes deposited using water vapor as a reactive plasma constituent
AbstractThe deposition and properties of sputtered iridium oxide films (SIROFs) using water vapor as a reactive gas constituent are investigated for their potential as high ‐charge‐capacity neural stimulation electrodes. Systematic investigation through a series of optical and electrochemical measurements reveals that the incorporation of water vapor as a reactive gas constituent, along with oxygen, alters the reduction–oxidation (redox) state of the plasma as we ll as its morphology and the electrochemical characteristics, including the cathodal charge‐storage capacity (CSCc) and charge ‐injection capacity (...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - July 28, 2019 Category: Materials Science Authors: Jimin Maeng, Bitan Chakraborty, Negar Geramifard, Tong Kang, Rashed T. Rihani, Alexandra Joshi ‐Imre, Stuart F. Cogan Tags: ORIGINAL RESEARCH REPORT Source Type: research

Freeze ‐dry processing of three‐dimensional cell constructs for bone graft materials
In this study, we found that sucrose‐containing phosphate buffer was suitable for freeze drying to maintain minerals and protein functions within 3D cell constructs, w hereas citrate buffer was inappropriate. The insights gained by this study may facilitate the development of novel cell‐based biomaterials fabricated by tissue engineering approaches and bone graft biomaterials. (Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials)
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - July 26, 2019 Category: Materials Science Authors: Jun ‐Ichi Sasaki, Itsumi Yoshimoto, Chihiro Katata, Ririko Tsuboi, Satoshi Imazato Tags: ORIGINAL RESEARCH REPORT Source Type: research

Synthesis, characterization, and cytocompatibility of yttria stabilized zirconia nanopowders for creating a window to the brain
In this study, a new pr ocess using aerosol spray pyrolysis was established for synthesizing 6–7 nm YSZ nanopowders with precisely controlled compositions. YSZ nanopowders with 3 M ratios of yttria to zirconia, specifically 3, 6, and 8% yttria in zirconia (referred to as 3YSZ, 6YSZ, and 8YSZ, respectively) were synthesiz ed and characterized. The size, structure, and composition of the produced YSZ nanoparticles are highly controllable and scalable. The in vitro cytocompatibility of the YSZ nanoparticles with bone marrow mesenchymal stem cells (BMSCs) was investigated using a direct exposure culture method for crani ...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - July 24, 2019 Category: Materials Science Authors: Dana Rutherford, Stephen Exarhos, Changlu Xu, Matt Niacaris, Crystal Mariano, Bryce Dayap, Lorenzo Mangolini, Huinan Liu Tags: ORIGINAL RESEARCH REPORT Source Type: research