Evaluation of rabbit adipose derived stem cells fate in perfused multilayered silk fibroin composite scaffold for Osteochondral repair
In conclusion, applying flow perfusion on the prepared integrated trilayered SF-based scaffold can support osteogenic and chondrogenic differentiation for repairing osteochondral defects. (Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials)
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - March 5, 2024 Category: Materials Science Authors: Sara Zadegan, Bahman Vahidi, Jhamak Nourmohammadi, Asiyeh Shojaee, Nooshin Haghighipour Tags: RESEARCH ARTICLE Source Type: research

Development and optimization of kaempferol loaded ethosomes using Box –Behnken statistical design: In vitro and ex‐vivo assessments
AbstractKaempferol (KMP) belong to flavonoid class have developed in ethosomal formulation and were evaluated for their potential to treat diabetic foot ulcers. Even though ethosomes are highly deformable, they can pass through human skin intact. KMP ethosomes were formulated using the cold method and optimized by Box –Behnken design (BBD) (three-factor, three-level (33)). The formulation variables used for optimization are drug concentration of KMP, soylecithin content, and ethanol percentage. The optimized formulation was examined using transmission electronic microscopy (TEM), differential scanning calorimetry (DSC), ...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - March 4, 2024 Category: Materials Science Authors: Shraddha Singh Raghav, Bhavna Kumar, Neeraj Kumar Sethiya, Shilpa Pahwa Tags: RESEARCH ARTICLE Source Type: research

Zinc ‐doped hydroxyapatite as a pH responsive drug delivery system for anticancer drug 6‐mercaptopurine
Abstract6-Mercaptopurine (6MP) is commonly used in the treatment of acute lymphoblastic leukemia as an important agent in maintenance therapy. Despite its therapeutic benefits, 6MP has some limitations during therapy. Taking into account the disadvantages during 6MP therapy, there is a great need to create an appropriate delivery system for this drug. 6MP contains in its structure nitrogen and sulfur atoms capable of forming coordination compounds with metal ions, for example zinc. Therefore, in this work, we prepared biocompatible hydroxyapatite (HAp) doped with zinc ions, and used it as a carrier for 6MP. Doped HAp has n...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - March 4, 2024 Category: Materials Science Authors: Marcel Jakubowski, Łukasz Majchrzycki, Aleksej Zarkov, Adam Voelkel, Mariusz Sandomierski Tags: RESEARCH ARTICLE Source Type: research

Development of guar gum reinforced calcium magnesium phosphate ‐based bone biocement
This study aims at developing a calcium magnesium phosphate-based bone biocement that combines a natural polymer and regenerative properties of bone bonding materials. The formulation of this biocement consists of oxidized guar gum, polydopamine, and calcium magnesium phosphate. The oxidized guar gum is easily soluble in water and has a slightly basic pH, unlike unmodified guar gum, thus allowing a homogenous paste to form in the alkaline environment of calcium magnesium phosphate. Three different oxidized degrees of guar gum were made, and the impact on the biocement properties was studied. The modified guar gum-reinforce...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - February 25, 2024 Category: Materials Science Authors: Vidul Goenka, Anupama Devi V. K., Ceera Manikandan, Amit Kumar Jaiswal Tags: RESEARCH ARTICLE Source Type: research

Studying the electrical, mechanical, and biological properties of BCZT –HA composites
AbstractThe piezoelectric properties of natural bone and their influence on bone growth have inspired researchers to study a range of bio-piezoelectric composite materials. By exploring these materials, researchers aim to understand better, how piezoelectricity can be controlled to promote bone growth and tissue regeneration. In this work, the prominent piezoelectric material, (Ba, Zr) TiO3-x(Ba,Ca)TiO3, abbreviated as BCZT, was selected as a possible bone growth enhancer in hydroxyapatite (HA) scaffolds. Initially, BCZT and hydroxyapatite (HA) powders were synthesized using the sol –gel method. Subsequently, various com...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - February 23, 2024 Category: Materials Science Authors: Fatemeh Zare Dehnov, Raziye Hayati, Lobat Tayebi Tags: RESEARCH ARTICLE Source Type: research

Strontium ‐loaded 3D intramedullary nail titanium implant for critical‐sized femoral defect in rabbits
In this study, an intramedullary nail shaped three-dimensional (3D)-printed porous titanium implant that is capable of releasing strontium ions was developed through a simple and cost-effective surface modification technique. The feasibility of this implant as a stand-alone solution was evaluated using a rabbit's segmental diaphyseal as a defect model. The strontium-loaded implant exhibited a favorable environment for cell adhesion, and mechanical properties that were commensurate with those of a rabbit's cortical bone. Radiographic, biomechanical, and histological analyses revealed a significantly higher amount of bone in...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - February 22, 2024 Category: Materials Science Authors: Shintaro Honda, Shunsuke Fujibayashi, Takayoshi Shimizu, Seiji Yamaguchi, Yaichiro Okuzu, Yusuke Takaoka, Soichiro Masuda, Mitsuru Takemoto, Toshiyuki Kawai, Bungo Otsuki, Koji Goto, Shuichi Matsuda Tags: RESEARCH ARTICLE Source Type: research

Issue Information
(Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials)
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - February 20, 2024 Category: Materials Science Tags: ISSUE INFORMATION Source Type: research

Combination of in silico and cell culture strategies to predict biomaterial performance: Effects of sintering temperature on the biological properties of hydroxyapatite
In this study, differential gene expression analyses were performed using the gene set variat ion analysis (GSVA) algorithm from the transcriptomes respecting the thermal changes of HA, which were validated using exclusively in vitro strategies. Furthermore, in silico approaches elected biomarkers during cell behavior in response to different sintering temperatures of HA, and it was further validated using cell culture and qPCR technologies. Altogether, the combination of those strategies shows the capacity of sintered HA at 1250°C to present a better performance in organizing an adequate microenvironment favoring bone re...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - February 15, 2024 Category: Materials Science Authors: Gerson S. de Almeida, Marcel R. Ferreira, C élio C. Fernandes Jr, Carlos A. O. de Biagi Jr, Wilson Araújo Silva Jr, Elidiane C. Rangel, Paulo N. Lisboa‐Filho, Willian F. Zambuzzi Tags: RESEARCH ARTICLE Source Type: research

Functionalization of a zirconia surface by covalently immobilized fibronectin and its effects on resistance to thermal, acid, and mechanical exposure
In this study, we investigated the stability of the 3-aminopropyldiisopropylethoxysilane (APDS) layer on yttria-partially stabilized zirconia (Y-TZP) surfaces after mechanical, acid, and thermal treatment in order to simulate fluctuations within the oral cavity. To accomplish that, the viability of human gingival fibroblasts on APDS-modified surfaces after applied treatment strategies was assessed by live/dead staining. Moreover, the hydrolysis stability and enzymatic degradation resistance of crosslinked fibronectin to the APDS layer was examined by immunostaining and western blot. The results revealed that the applied mo...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - February 14, 2024 Category: Materials Science Authors: Alena L. Palkowitz, Taskin Tuna, Robert Kaufmann, Eva Miriam Buhl, Stefan Wolfart, Horst Fischer Tags: RESEARCH ARTICLE Source Type: research

Osseointegration of implant surfaces in metabolic syndrome and type ‐2 diabetes mellitus
AbstractThisin vivo study evaluated the bone healing response around endosteal implants with varying surface topography/chemistry in a preclinical, large transitional model induced with metabolic syndrome (MS) and type-2 diabetes mellitus (T2DM). Fifteen G öttingen minipigs were randomly distributed into two groups: (i) control (normal diet,n = 5) and (ii) O/MS (cafeteria diet for obesity induction,n = 10). Following obesity induction, five minipigs from the obese/metabolic syndrome (O/MS) group were further allocated, randomly, into the third experimental group: (iii) T2DM (cafeteria diet + streptozotocin). ...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - February 14, 2024 Category: Materials Science Authors: Edmara T. P. Bergamo, Paula G. F. P. de Oliveira, Tiago M. B. Campos, Estevam A. Bonfante, Nick Tovar, Daniel Boczar, Vasudev Vivekanand Nayak, Paulo G. Coelho, Lukasz Witek Tags: RESEARCH ARTICLE Source Type: research

Combining PLGA microspheres loaded with Liver X receptor agonist GW3965 with a chitosan nerve conduit can promote the healing and regeneration of the wounded sciatic nerve
AbstractGlobally, peripheral nerve injury (PNI) is a common clinical issue. Successfully repairing severe PNIs has posed a major challenge for clinicians. GW3965 is a highly selective LXR agonist, and previous studies have demonstrated its positive protective effects in both central and peripheral nerve diseases. In this work, we examined the potential reparative effects of GW3965-loaded polylactic acid co-glycolic acid microspheres in conjunction with a chitosan nerve conduit for peripheral nerve damage. The experiment revealed that GW3965 promoted Schwann cell proliferation and neurotrophic factor release in vitro. In vi...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - February 14, 2024 Category: Materials Science Authors: Yuanwu Zhong, Shiqi Li, Yanzhen Chen, Yuan Tang, Xinmao Xiao, Tao Nie Tags: RESEARCH ARTICLE Source Type: research

In vivo behavior in rabbit radius bone defect of scaffolds based on nanocarbonate hydroxyapatite
In this study, we studied the bone-inducing capacity of nCHA-based scaffolds alone (SAG) and enriched with osteostatin (SAGO) or with bone marrow aspirate(SAGB) after implantation for 12  weeks in a 15-mm long critical defect performed in the radius of New Zealand rabbits. Bone formation obtained was compared with a group with the unfilled defect (CE), as control group, and other with the defect filed with iliac crest autograft (GS), as gold standard. X-ray follow-up was performed at 2, 4, 6 and 12 weeks and μCT and histological studies at 12 weeks. The radiological results showed a greater increment in bone formati...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - February 13, 2024 Category: Materials Science Authors: Lorena Garc ía‐Lamas, Juan Peña, Jesús Roman, Victoria Cabañas, Beatriz Bravo‐Giménez, Verónica Jiménez‐Díaz, Sandra Sánchez‐Salcedo, Javier Jiménez‐Holguín, Monica Abella, Manuel Desco, Daniel Lozano, David Cecilia‐Lópe Tags: RESEARCH ARTICLE Source Type: research

Degrees of macrophage ‐facilitated healing in aneurysm occlusion devices
AbstractInsufficient healing of aneurysms following treatment with vascular occlusion devices put patients at severe risk of fatal rupture. Therefore, promoting healing and not just occlusion is vital to enhance aneurysm healing. Following occlusion device implantation, healing is primarily orchestrated by macrophage immune cells, ending with fibroblasts depositing collagen to stabilize the aneurysm neck and dome, preventing rupture. Several modified occlusion devices are available currently on-market. Previous in vivo work demonstrated that modifications of occlusion devices with a shape memory polymer foam had enhanced a...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - February 12, 2024 Category: Materials Science Authors: Del A. Donehoo, Claudia A. Collier, Sabrina N. VandenHeuvel, Sanjana Roy, Spencer C. Solberg, Shreya A. Raghavan Tags: RESEARCH ARTICLE Source Type: research

Systematic enhancement of microbial decontamination efficiency in bone graft processing by means of high hydrostatic pressure using Escherichia coli as a model organism
This study aims to investigate the ability of high-pressure decontamination and to establish a treatment protocol that is able to successfully inactivate microorganisms with the final goal to sterilize bone specimens. UsingEscherichia coli (E. coli) as a model organism, HHP treatment parameters like temperature and duration, pressurization medium, and the number of treatment cycles were systematically adjusted to maximize the efficiency of inactivating logarithmic and stationary phase bacteria. Towards that we quantified colony-forming units (cfu) after treatment and investigated morphological changes via Field Emission Sc...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - February 12, 2024 Category: Materials Science Authors: Henrike Loeffler, Janine Waletzko ‐Hellwig, Ralf‐Joerg Fischer, Mirko Basen, Marcus Frank, Anika Jonitz‐Heincke, Rainer Bader, Annett Klinder Tags: RESEARCH ARTICLE Source Type: research

Characterization of residual debris on packaged hip arthroplasty stems demonstrates the dominance of less than 10  μm sized particulate: Updated USP788 guidelines for orthopedic implants
AbstractPast evaluation of particle contamination on packaged implants has typically been conducted using US Pharmacopeia (USP) 788, a 1970s pharmaceutical guideline created to evaluate contaminant particles in injectable fluids and syringes. Our objective was to reestablish relevant acceptance criteria for residual orthopedic and other implant debris, including smaller particles (i.e.,<10  μm in diameter). Packaged total hip arthroplasty (THA) titanium (Ti6Al4V)-alloy femoral stems were used (hydroxyapatite [HA]-coated and non-coated stems). Short-term ultrasonication and longer-term 24-hour soak/agitation methods w...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - February 11, 2024 Category: Materials Science Authors: Nadim J. Hallab, Salem R. Hallab, Anastasia Alexander, Robin Pourzal Tags: RESEARCH ARTICLE Source Type: research