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Total 41092 results found since Jan 2013.
Combination of siRNA-directed Kras oncogene silencing and arsenic-induced apoptosis using a nanomedicine strategy for the effective treatment of pancreatic cancer
Abstract: The synergetic inhibitory effects on human pancreatic cancer by nanoparticle-mediated siRNA and arsenic therapy were investigated both in vitro and in vivo. Poly(ethylene glycol)-block-poly(l-lysine) were prepared to form siRNA-complexed polyplex and poly(ethylene glycol)-block-poly(dl-lactide) were prepared to form arsenic-encapsulated vesicle, respectively. Down-regulation of the mutant Kras gene by siRNA caused defective abilities of proliferation, clonal formation, migration, and invasion of pancreatic cancer cells, as well as cell cycle arrest at the G0/G1 phase, which substantially enhanced the apoptosis-in...
Source: Nanomedicine : Nanotechnology, Biology, and Medicine - September 11, 2013 Category: Nanotechnology Authors: Linjuan Zeng, Jingguo Li, Yong Wang, Chenchen Qian, Yinting Chen, Qiubo Zhang, Wei Wu, Zhong Lin, Jianzhong Liang, Xintao Shuai, Kaihong Huang Tags: Pathology, Neoplasms, PanC, Kras, [(siRNA)-PEG-PLL], [(Arsenic)-PEG-PDLL] Co-administration Source Type: research
Hybrid lipid–polymer nanoparticles for sustained siRNA delivery and gene silencing
Abstract: The development of controlled-release nanoparticle (NP) technologies has great potential to further improve the therapeutic efficacy of RNA interference (RNAi), by prolonging the release of small interfering RNA (siRNA) for sustained, long-term gene silencing. Herein, we present an NP platform with sustained siRNA-release properties, which can be self-assembled using biodegradable and biocompatible polymers and lipids. The hybrid lipid–polymer NPs showed excellent silencing efficacy, and the temporal release of siRNA from the NPs continued for over one month. When tested on luciferase-expressed HeLa cells and A...
Source: Nanomedicine : Nanotechnology, Biology, and Medicine - March 19, 2014 Category: Nanotechnology Authors: Jinjun Shi, Yingjie Xu, Xiaoyang Xu, Xi Zhu, Eric Pridgen, Jun Wu, Alexander R. Votruba, Archana Swami, Bruce R. Zetter, Omid C. Farokhzad Tags: Pharmacology, Nano-Enabled Drug Delivery, Controlled Release, Polymer-siRNA Hybrid Source Type: research
Comparative cellular pharmacokinetics and pharmacodynamics of siRNA delivery by SPANosomes and by cationic liposomes
Abstract: Mechanistic understanding of intracellular trafficking is important for the development of small interfering RNA (siRNA) delivery vehicles. Here, we describe a novel methodology to quantitatively analyze nanocarrier-mediated disposition of siRNA. Cellular uptake and cytoplasmic release of siRNA over time were quantified by measuring the fluorescence intensities of fluorescently-labeled siRNAs and molecular beacons using flow cytometry. This method was used to investigate the cellular pharmacokinetics (PK) of siRNA delivery by SPANosomes (SP) and by cationic liposomes (CL). The results showed that the superior pha...
Source: Nanomedicine : Nanotechnology, Biology, and Medicine - October 31, 2012 Category: Nanotechnology Authors: Chenguang Zhou, Yue Zhang, Bo Yu, Mitch A. Phelps, L. James Lee, Robert J. Lee Tags: Pharmaceutics, siRNA Delivery, PKPD, Liposomes Source Type: research
i-Fect Delivers siRNA in vitro and in vivo
In this study data suggest the protective effects of EPO on NUV injuries are highly associated with the increase of p-Cx43, which improves GJIC to reduce neurotoxic substances: Ziyi Zhoua, Xiaobai Weib, Jun Xiang, Junpeng Gao, Lixin Wang, Jinsong You, Yefeng Cai , Dingfang Caid. Protection of erythropoietin against ischemic neurovascular unit injuries through the effects of connexin43. Biochemical and Biophysical Research Communications. doi:10.1016/j.bbrc.2015.02...The strands were incubated at 90°C for 5 min and then at 37°C for 1 h. SiRNA was prepared immediately before administration by mixing the RNA solution (1 μg...
Source: Neuromics - February 21, 2015 Category: Neuroscience Tags: Cerebral Ischemia Connexin 43 Delivering siRNA to the CNS EPO HDAC2 i-Fect Stroke Source Type: news
Using i-Fect to Knock Down GLT-1 Gene in vivo
Pain Researchers continue to use our i-FectTM Transfection Kit for Gene Expression AnalysisValproate Prevents Dysregulation of Spinal Glutamate and Reduces the Development of Hypersensitivity in Rats After Peripheral Nerve Injury. The Journal of Pain, Volume 14, Issue 11, November 2013, Pages 1485–1491 http://dx.doi.org/10.1016/j.jpain.2013.07.007.Researchers use the kit to deliver Glutamate Receptor 1 siRNA in vivo.The present study examined whether the histone deacetylase inhibitor valproate prevents downregulation of glutamate transporters in the primary cultured astrocytes and in the spinal cord after L5-L6 spinal ne...
Source: siRNA and DsiRNA Transfection Efficiency - November 6, 2013 Category: Neuroscience Tags: Delivering siRNA to the CNS GLT-1 Gene Glutamate Receptor 1 siRNA in vivo i-Fect intrathecal delivery of siRNA Source Type: news
Neuromics' Transfection Kits-Genes Studied
Delivering siRNA, miRNA, Plasmids and Viral Vectors for Gene Expression Analysis.I have shared the many genes researchers have studied using our Transfection Kits. These include: β-arrestin, ABCA, ASIC, β-arrestin, CAV1.2, CX3CR1, DOR, EHDAC2, LOVL4, IKBKAP, K+-ATPase, KV1.1, KV9.1 , neuroligin 2, The β3 subunit of the Na+,K+-ATPase, NTS1, NAV1.8, NTS1, NOV, Raf-1, RANK, SNSR1, hTert, NOV, Survivin, TLR4, Troy and TRPV1 and More!We can now add GPNMB to this list: Lili Hou, Yanfeng Zhang, Yong Yang, Kai Xiang, Qindong Tan, Qulian Guo. Intrathecal siRNA Against GPNMB Attenuates Nociception in a Rat Model of Neuropath...
Source: siRNA and DsiRNA Transfection Efficiency - July 23, 2014 Category: Neuroscience Tags: Delivering siRNA to the CNS Gene Expression Analysis Gene Silencing i-Fect in vivo siRNA pn-Fect tansfection Transfection Kits Source Type: news
Neuromics ' Transfection Kits-Genes Studied
Delivering siRNA, miRNA, Plasmids and Viral Vectors for Gene Expression Analysis . I have shared the many genes researchers have studied using our Transfection Kits . These include: β-arrestin, ABCA, ASIC, β-arrestin, CAV1.2, CX3CR1, DOR, EHDAC2, LOVL4, IKBKAP, K+-ATPase, KV1.1, KV9.1 , neuroligin 2, The β3 subunit of the Na+,K+-ATPase, NTS1, NAV1.8, NTS1, NOV, Raf-1, RANK, SNSR1, hTert, NOV, Survivin, TLR4, Troy and TRPV1 and More! We can now add GPNMB to this list: Lili Hou, Yanfeng Zhang, Yong Yang, Kai Xiang, Qindong Tan, Qulian Guo. Intrathecal siRNA Against GPNMB Attenuates Nociception in a Rat Model of N...
Source: siRNA and DsiRNA Transfection Efficiency - July 23, 2014 Category: Neuroscience Tags: Delivering siRNA to the CNS Gene Expression Analysis Gene Silencing i-Fect in vivo siRNA pn-Fect tansfection Transfection Kits Source Type: news
High efficacy gold-KDEL peptide-siRNA nanoconstruct-mediated transfection in C2C12 myoblasts and myotubes
Abstract: Gold nanoparticles (AuNP) were conjugated with cysteine terminated KDEL (Lys-Asp-Glu-Leu) peptide and siRNA directed against NADPH Oxidase 4 (Nox4). Fluorescence microscopy analysis provided evidence of cytocellular retrograde transport pathways and sub-cellular colocalization of AuNP nanoconstructs in both undifferentiated C2C12 myoblasts and differentiated C2C12 myotubes. The cellular trafficking of AuNP nanoconstructs in undifferentiated myoblasts suggests stable and efficient transfection of siRNA as demonstrated by colocalization of AuNP-delivered KDEL and siRNA. The cellular uptake of AuNP nanoconstructs wa...
Source: Nanomedicine : Nanotechnology, Biology, and Medicine - August 8, 2013 Category: Nanotechnology Authors: Suresh Acharya, Rodney Allan Hill Tags: Cell Biology, Intracellular Transport Mechanism, Sirna-Au-KDEL Peptide Nanoconstructs Source Type: research
i-Fect, BDNF and Irritable Bowel Syndrome (IBS)
Conclusion: Chronic prenatal stress followed by a second exposure to HeICS in adult offspring exacerbated visceral hypersensitivity (VHS) greater in female offspring that persisted longer than in male offspring. Chronic prenatal stress up-regulated BDNF expression in the lumbar-sacral dorsal horn that correlated with the exacerbation of VHS in female, but not in male offspring by increasing RNA Pol II binding and histone H3 acetylation, and decreasing histone deacetylase 1 association with the core promoter of BDNF in female offspring.
Source: siRNA and DsiRNA Transfection Efficiency - March 11, 2014 Category: Neuroscience Tags: BDNF;chronic stress;functional bowel disorders;iritable bowel syndrome;visceral hypersnsitivity i-Fect intrathecal delivery of siRNA Source Type: news
Preclinical development of siRNA therapeutics: Towards the match between fundamental science and engineered systems
Abstract: The evolution of synthetic RNAi faces the paradox of interfering with the human biological environment. Due to the fact that all cell physiological processes can be target candidates, silencing a precise biological pathway could be challenging if target selectivity is not properly addressed. Molecular biology has provided scientific tools to suppress some of the most critical issues in gene therapy, while setting the standards for siRNA clinical application. However, the protein down-regulation through the mRNA silencing is intimately related to the sequence-specific siRNA ability to interact accurately with the ...
Source: Nanomedicine : Nanotechnology, Biology, and Medicine - December 11, 2013 Category: Nanotechnology Authors: M. Videira, A. Arranja, D. Rafael, R. Gaspar Tags: Cell Biology and Genetics, Amino Acids, Nucleic Acids, siRNA Therapy, Basic Science vs. Engineering Source Type: research
EPO Protects Against Cerebral Ischemia
In this study data suggest the protective effects of EPO on NUV injuries are highly associated with the increase of p-Cx43, which improves GJIC to reduce neurotoxic substances: Ziyi Zhoua, Xiaobai Weib, Jun Xiang, Junpeng Gao, Lixin Wang, Jinsong You, Yefeng Cai , Dingfang Caid. Protection of erythropoietin against ischemic neurovascular unit injuries through the effects of connexin43. Biochemical and Biophysical Research Communications. doi:10.1016/j.bbrc.2015.02...The strands were incubated at 90°C for 5 min and then at 37°C for 1 h. SiRNA was prepared immediately before administration by mixing the RNA solution (1 μg...
Source: siRNA and DsiRNA Transfection Efficiency - February 18, 2015 Category: Neuroscience Tags: cerebral ischemia Connexin 43 Delivering siRNA to the CNS i-Fect iFect siRNA transfection of neurons Source Type: news
Polymeric delivery of siRNA for dual silencing of Mcl-1 and P-glycoprotein and apoptosis induction in drug-resistant breast cancer cells
& H Uludağ
Source: Cancer Gene Therapy - March 1, 2013 Category: Cancer & Oncology Authors: H M AliabadiP MahdipoorH Uludağ Tags: lipophilic polymers Mcl-1 multidrug resistance P-glycoprotein siRNA delivery Source Type: research
Current Development of siRNA Bioconjugates: From Research to the Clinic
In this study, it was shown that the main factor determining the nature of the biodistribution of conjugates is their lipophilicity. Conjugates of siRNA with lower lipophilicity; i.e., derivatives of retinoic acid, lithocholic acid, and docosahexanoic acid with greater efficiency than cholesterol conjugates accumulated in the kidneys, bladder, and lungs of the mouse after subcutaneous injection (Biscans et al., 2018). This fact is consistent with previous data that showed that more lipophilic conjugates bind more efficiently to serum components, and thus are not excreted by the kidneys (Wolfrum et al., 2007; Osborn et al.,...
Source: Frontiers in Pharmacology - April 25, 2019 Category: Drugs & Pharmacology Source Type: research
Tumor-specific delivery of siRNA using supramolecular assembly of hyaluronic acid nanoparticles and 2b RNA-binding protein/siRNA complexes.
Abstract
Anticancer therapeutics delivering exogenous siRNA have been explored to suppress the tumor-associated genes, but several limitations of siRNA delivery such as tumor-targeted delivery, controlled siRNA release at the sites of interest, or instabilities of siRNA in physiological fluids should be preferentially addressed for its clinical applications. As an attempt to meet these criteria, we designed a supramolecular assembly, which was composed of cholesterol-bearing hyaluronic acid (HA-Chol) conjugates and 2b RNA-binding protein (2b)/siRNA complexes. In contrast to the traditional siRNA polyplexes using e...
Source: Biomaterials - May 19, 2014 Category: Materials Science Authors: Choi KM, Jang M, Kim JH, Ahn HJ Tags: Biomaterials Source Type: research
SiRNA-phospholipid conjugates for gene and drug delivery in cancer treatment.
In this study, the thiol-modified sense and antisense siRNA are chemically conjugated with phospholipids to form sense and antisense siRNA-phospholipid, and then these sense or antisense siRNA-phospholipids with equal amounts are annealed to generate siRNA-phospholipids. The siRNA-phospholipids can serve dual functions as agents that can silence gene expression and as a component of nanoparticles to embed hydrophobic anticancer drugs to cure tumor. siRNA-phospholipids together with cationic lipids and DSPE-PEG2000 fuse around PLGA to form siRNA-phospholipids enveloped nanoparticles (siRNA-PCNPs), which can deliver siRNAs a...
Source: Biomaterials - May 2, 2014 Category: Materials Science Authors: Liu H, Li Y, Mozhi A, Zhang L, Liu Y, Xu X, Xing J, Liang X, Ma G, Yang J, Zhang X Tags: Biomaterials Source Type: research
