Filtered By:
Source: Viruses

This page shows you your search results in order of date. This is page number 13.

Order by Relevance | Date

Total 1003 results found since Jan 2013.

Viruses, Vol. 15, Pages 300: A Serological Investigation of Porcine Reproductive and Respiratory Syndrome and Three Coronaviruses in the Campania Region, Southern Italy
ena Montagnaro Porcine coronaviruses and reproductive and respiratory syndrome (PRRS) are responsible for severe outbreaks that cause huge economic losses worldwide. In Italy, three coronaviruses have been reported historically: porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV) and porcine respiratory coronavirus (PRCV). Although repeated outbreaks have been described, especially in northern Italy, where intensive pig farming is common, there is a worrying lack of information on the spread of these pathogens in Europe. In this work, we determined the seroprevalence of three porcine coro...
Source: Viruses - January 20, 2023 Category: Virology Authors: Gianmarco Ferrara Emanuele D ’Anza Antonella Rossi Elvira Improda Valentina Iovane Ugo Pagnini Giuseppe Iovane Serena Montagnaro Tags: Brief Report Source Type: research

Viruses, Vol. 15, Pages 287: Quinazolinone-Peptido-Nitrophenyl-Derivatives as Potential Inhibitors of SARS-CoV-2 Main Protease
In this study, a fragment-based strategy including potential antiviral quinazolinone moiety and glutamine- or glutamate-derived peptidomimetic backbone and positioned nitro functional groups was used to synthesize putative Mpro inhibitors. Two compounds, G1 and G4, exhibited anti-Mpro enzymatic activity in a dose-dependent manner, with the calculated IC50 values of 22.47 ± 8.93 μM and 24.04 ± 0.67 μM, respectively. The bio-layer interferometer measured real-time binding. The dissociation kinetics of G1/Mpro and G4/Mpro also showed similar equilibrium dissociation constant...
Source: Viruses - January 19, 2023 Category: Virology Authors: Huynh-Nguyet-Huong Giang Feng-Pai Chou Ching-Yun Chen Shen-Chieh Chou Sheng-Cih Huang Tuoh Wu Bui-Thi-Buu Hue Hong-Cheu Lin Tung-Kung Wu Tags: Article Source Type: research

Viruses, Vol. 15, Pages 290: Protein Arginylation Is Regulated during SARS-CoV-2 Infection
Conclusions: We demonstrate that ATE1 is increased during SARS-CoV-2 infection and its inhibition has potential therapeutic value.
Source: Viruses - January 19, 2023 Category: Virology Authors: Janaina Macedo-da-Silva Livia Rosa-Fernandes Vinicius de Morais Gomes Veronica Feijoli Santiago Deivid Martins Santos Catarina Maria Stanischesk Molnar Bruno Rafael Barboza Edmarcia Elisa de Souza Rodolfo Ferreira Marques Silvia Beatriz Boscardin Edison L Tags: Article Source Type: research

Viruses, Vol. 15, Pages 264: Unique Variants of Avian Coronaviruses from Indigenous Chickens in Kenya
David L. Suarez The avian gamma-coronavirus infectious bronchitis virus (AvCoV, IBV; Coronaviridae family) causes upper respiratory disease associated with severe economic losses in the poultry industry worldwide. Here, we report for the first time in Kenya and the Eastern African region two novel AvCoVs, designated IBV/ck/KE/1920/A374/2017 (A374/17) and AvCoV/ck/KE/1922/A376/2017 (A376/17), inadvertently discovered using random nontargeted next-generation sequencing (NGS) of cloacal swabs collected from indigenous chickens. Despite having genome organization (5′UTR-[Rep1a/1ab-S-3a-3b-E-M-4b-4c-5a-5b-N-...
Source: Viruses - January 17, 2023 Category: Virology Authors: Henry M. Kariithi Jeremy D. Volkening Iryna V. Goraichuk Leonard O. Ateya Dawn Williams-Coplin Tim L. Olivier Yatinder S. Binepal Claudio L. Afonso David L. Suarez Tags: Article Source Type: research

Viruses, Vol. 15, Pages 245: Detection and Molecular Characterization of the SARS-CoV-2 Delta Variant and the Specific Immune Response in Companion Animals in Switzerland
In conclusion, our study is the first to describe the SARS-CoV-2 Delta variant transmission to animals in Switzerland and provides the first-ever description of Delta-variant pangolin lineages AY.129 and AY.4 in animals. Our results reinforce the need of a One Health approach in the monitoring of SARS-CoV-2 in animals.
Source: Viruses - January 15, 2023 Category: Virology Authors: Evelyn Kuhlmeier Tatjana Chan Cecilia Valenzuela Ag üí Barbara Willi Aline Wolfensberger Christian Beisel Ivan Topolsky Niko Beerenwinkel Tanja Stadler Swiss SARS-CoV-2 Sequencing Consortium Swiss SARS-CoV-2 Sequencing Consortium Sarah Jones Grace Tyson Tags: Article Source Type: research

Viruses, Vol. 15, Pages 250: Chetomin, a SARS-CoV-2 3C-like Protease (3CLpro) Inhibitor: In Silico Screening, Enzyme Docking, Molecular Dynamics and Pharmacokinetics Analysis
med-Elamir F. Hegazy The emergence of the Coronavirus Disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has led to over 6 million deaths. The 3C-like protease (3CLpro) enzyme of the SARS-CoV-2 virus is an attractive druggable target for exploring therapeutic drug candidates to combat COVID-19 due to its key function in viral replication. Marine natural products (MNPs) have attracted considerable attention as alternative sources of antiviral drug candidates. In looking for potential 3CLpro inhibitors, the MNP database (>14,000 molecules) was virtually scre...
Source: Viruses - January 15, 2023 Category: Virology Authors: Mahmoud A. A. Ibrahim Alaa H. M. Abdelrahman Dina E. M. Mohamed Khlood A. A. Abdeljawaad Mohamed Ahmed Naeem Gamal A. Gabr Ahmed M. Shawky Mahmoud E. S. Soliman Peter A. Sidhom Paul W. Par é Mohamed-Elamir F. Hegazy Tags: Article Source Type: research

Viruses, Vol. 15, Pages 223: Disappearance and Re-Emergence of Influenza during the COVID-19 Pandemic: Association with Infection Control Measures
In this study, the associations between infection control measures taken for COVID-19 and the global disappearance of the influenza virus were investigated. The detection rate of influenza from baseline was investigated during four seasons (12 weeks from epidemiological week 49 in 2020 and 2021 and 12 weeks from epidemiological week 23 in 2020 and 2021) in each country participating in the surveillance system of the World Health Organization. Three measures of infection control: mask use ratio, social distancing index (an index of human mobility and physical distance obligations), and an index of stringency of measures tak...
Source: Viruses - January 13, 2023 Category: Virology Authors: Hikaru Takeuchi Ryuta Kawashima Tags: Article Source Type: research

Viruses, Vol. 15, Pages 215: Proline & ndash;Proline Dyad in the Fusion Peptide of the Murine & beta; & ndash;Coronavirus Spike Protein & rsquo;s S2 Domain Modulates Its Neuroglial Tropism
Viruses, Vol. 15, Pages 215: Proline–Proline Dyad in the Fusion Peptide of the Murine β–Coronavirus Spike Protein’s S2 Domain Modulates Its Neuroglial Tropism Viruses doi: 10.3390/v15010215 Authors: Safiriyu Mulchandani Anakkacheri Pal Das Sarma The β-Coronavirus mouse hepatitis virus (MHV-A59)-RSA59 has a patent stretch of fusion peptide (FP) containing two consecutive central prolines (PP) in the S2 domain of the Spike protein. Our previous studies compared the PP-containing fusogenic-demyelinating strain RSA59(PP) to its one proline-deleted mutant ...
Source: Viruses - January 12, 2023 Category: Virology Authors: Safiriyu Mulchandani Anakkacheri Pal Das Sarma Tags: Article Source Type: research

Viruses, Vol. 15, Pages 213: Multi-Targeting Approach in Selection of Potential Molecule for COVID-19 Treatment
dra Nayak The coronavirus disease (COVID-19) is a pandemic that started in the City of Wuhan, Hubei Province, China, caused by the spread of coronavirus (SARS-CoV-2). Drug discovery teams around the globe are in a race to develop a medicine for its management. It takes time for a novel molecule to enter the market, and the ideal way is to exploit the already approved drugs and repurpose them therapeutically. We have attempted to screen selected molecules with an affinity towards multiple protein targets in COVID-19 using the Schrödinger suit for in silico predictions. The proteins selected were angiotensin...
Source: Viruses - January 12, 2023 Category: Virology Authors: Varalakshmi Velagacherla Akhil Suresh Chetan Hasmukh Mehta Usha Y. Nayak Yogendra Nayak Tags: Article Source Type: research

Viruses, Vol. 15, Pages 212: Reduced Seasonal Coronavirus Antibody Responses in Children Following COVID-19 Mitigation Measures, The Netherlands
Pieter L. A. Fraaij SARS-CoV-2 prevention and control measures did not only impact SARS-CoV-2 circulation, but also the timing and prevalence of other seasonal respiratory viruses. Especially in children, information on exposure and infections to seasonal coronaviruses as well as SARS-CoV-2 in the first year of the pandemic is largely lacking. Therefore, we set up a one-year serological survey in a large tertiary hospital in the Netherlands. We show that seasonal coronavirus seroprevalence significantly decreased in 2021 in children less than one year, most likely due to COVID-19 control measures. The SARS-CoV-2 seropr...
Source: Viruses - January 12, 2023 Category: Virology Authors: Reina S. Sikkema Erwin de Bruin Christian Ramakers Robbert Bentvelsen Wentao Li Berend-Jan Bosch Brenda Westerhuis Bart Haagmans Marion P. G. Koopmans Pieter L. A. Fraaij Tags: Brief Report Source Type: research

Viruses, Vol. 15, Pages 204: The Adenovirus Vector Platform: Novel Insights into Rational Vector Design and Lessons Learned from the COVID-19 Vaccine
Ehrhardt The adenovirus vector platform remains one of the most efficient toolboxes for generation of transfer vehicles used in gene therapy and virotherapy to treat tumors, as well as vaccines to protect from infectious diseases. The adenovirus genome and capsids can be modified using highly efficient techniques, and vectors can be produced at high titers, which facilitates their rapid adaptation to current needs and disease applications. Over recent years, the adenovirus vector platform has been in the center of attention for vaccine development against the ongoing coronavirus SARS-CoV-2/COVID-19 pandemic. The worldw...
Source: Viruses - January 11, 2023 Category: Virology Authors: Erwan Sallard Wenli Zhang Malik Aydin Katrin Schr öer Anja Ehrhardt Tags: Review Source Type: research

Viruses, Vol. 15, Pages 174: Development and Characterization of Phage Display-Derived Monoclonal Antibodies to the S2 Domain of Spike Proteins of Wild-Type SARS-CoV-2 and Multiple Variants
In this study, four SARS-CoV-2 spike protein-specific single-chain variable fragments (scFvs) were isolated from a synthetic antibody library using phage display technology. Following the conversion of these scFvs into monoclonal antibodies (mAbs) (K104.1–K104.4) and production and purification of the mAbs, the antibody pair (K104.1 and K104.2) that exhibited the highest binding affinity (K104.1 and K104.2, 1.3 nM and 1.9 nM) was selected. Biochemical analyses revealed that this antibody pair specifically bound to different sites on the S2 subunit of the spike protein. Furthermore, we developed a highly sensi...
Source: Viruses - January 6, 2023 Category: Virology Authors: Ji Woong Kim Ah Hyun Cho Ha Gyeong Shin Sung Hoon Jang Su Yeon Cho Ye Rim Lee Sukmook Lee Tags: Article Source Type: research

Viruses, Vol. 15, Pages 162: Using Wastewater Surveillance to Compare COVID-19 Outbreaks during the Easter Holidays over a 2-Year Period in Cape Town, South Africa
In this study, the changes in the SARS-CoV-2 RNA levels in wastewater during Easter holidays in 2021 and 2022 in the City of Cape Town were monitored over nine weeks. Our findings showed a statistically significant difference in the SARS-CoV-2 RNA viral load between the study weeks over the Easter period in 2021 and 2022, except for study week 1 and 4. During the Easter week, 52% of the wastewater treatment plants moved from the lower (low viral RNA) category in 2021 to the higher (medium to very high viral RNA) categories in 2022. As a result, the median SARS-CoV-2 viral loads where higher during the Easter week in 2022 t...
Source: Viruses - January 5, 2023 Category: Virology Authors: Nomfundo Mahlangeni Ren ée Street Suranie Horn Angela Mathee Noluxabiso Mangwana Stephanie Dias Jyoti Rajan Sharma Pritika Ramharack Johan Louw Tarylee Reddy Swastika Surujlal-Naicker Sizwe Nkambule Candice Webster Mongezi Mdhluli Glenda Gray Christo Mul Tags: Article Source Type: research

Viruses, Vol. 15, Pages 134: A Cross-Sectional Comparative Characterization of Hematological Changes in Patients with COVID-19 Infection, Non-COVID Influenza-like Illnesses and Healthy Controls
Conclusion: We demonstrated morphological changes in neutrophils, monocytes and lymphocytes in COVID-19 infection and also non-COVID-19 Influenza-like illnesses with the help of VCS parameters. A cutoff for the absolute neutrophils count was able to differentiate COVID-19 infection requiring hospitalization from healthy controls and eosinopenia was a characteristic finding in cases with COVID-19 infection.
Source: Viruses - December 31, 2022 Category: Virology Authors: Mansi Kala Sohaib Ahmad Meghali Dhebane Kunal Das Manish Raturi Meghna Tyagi Anuradha Kusum Tags: Article Source Type: research

Viruses, Vol. 15, Pages 139: Monoclonal Antibodies Specific for SARS-CoV-2 Spike Protein Suitable for Multiple Applications for Current Variants of Concern
xander A. Khromykh Roy A. Hall Andreas Suhrbier Daniel J. Rawle Jody Hobson-Peters The global coronavirus disease 2019 (COVID-19) pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spawned an ongoing demand for new research reagents and interventions. Herein we describe a panel of monoclonal antibodies raised against SARS-CoV-2. One antibody showed excellent utility for immunohistochemistry, clearly staining infected cells in formalin-fixed and paraffin embedded lungs and brains of mice infected with the original and the omicron variants of SARS-CoV-2. We demonstrate the rea...
Source: Viruses - December 31, 2022 Category: Virology Authors: Mahali S. Morgan Kexin Yan Thuy T. Le Ryan A. Johnston Alberto A. Amarilla David A. Muller Christopher L. D. McMillan Naphak Modhiran Daniel Watterson James R. Potter Julian D. J. Sng Mary Lor Devina Paramitha Ariel Isaacs Alexander A. Khromykh Roy A. Hal Tags: Article Source Type: research