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Infectious Disease: Gastroenteritis
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Total 36196 results found since Jan 2013.
Identification and characterisation of a major outer membrane protein from Methylacidiphilum fumariolicum SolV
In this study, we identified and characterised a major outer membrane protein (WP_009059494) from Methylacidiphilum fumariolicum SolV. PRED-TMBB and AlphaFold2 predicted this protein to form a porin with a β-barrel structure consisting of ten antiparallel β-sheets and with a small amphipathic N-terminal α-helix in the periplasm. We purified soluble recombinant protein WP_009059494 from E. coli using Tris-HCl buffer with SDS. Antibodies were raised against two peptides in the two large extracellular loops of protein WP_009059494 and immunogold localisation showed this protein to be mainly present in the OM of strain SolV...
Source: Antonie van Leeuwenhoek - September 22, 2023 Category: Microbiology Authors: Changqing Liu Rob Mesman Arjan Pol Federica Angius Huub J M Op den Camp Source Type: research
Surface inoculation method impacts microbial reduction and transfer of Salmonella Enteritidis PT 30 and potential surrogates during dry sanitation
Int J Food Microbiol. 2023 Sep 18;406:110405. doi: 10.1016/j.ijfoodmicro.2023.110405. Online ahead of print.ABSTRACTDry sanitation methods are often limited to physical removal strategies such as brushing or wiping with sanitary cleaning tools. However, the relative efficacy of these approaches to remove microbiota on surfaces, and the risk of transferring cells to other surfaces via the cleaning tool, is unclear. The effect of dry wiping with a single-use towel on the removal of four different bacteria (Salmonella Enteritidis, Enterococcus faecium, Listeria innocua, Escherichia coli) was investigated. We also quantified t...
Source: International Journal of Food Microbiology - September 21, 2023 Category: Food Science Authors: Long Chen Abigail B Snyder Source Type: research
Identification of potential inhibitors against E.coli via novel approaches based on deep learning and quantum mechanics-based atomistic investigations
Arch Biochem Biophys. 2023 Sep 19:109761. doi: 10.1016/j.abb.2023.109761. Online ahead of print.ABSTRACTCurrently, drug resistance to commercially available antibiotics is imparting negative consequences to global health, and the development of novel antibiotics in a timely manner is a prime need of the hour. In the current study, an e-pharmacophore model was built using the 3D structure of DNA gyrase in complex with a standard inhibitor. The generated model was subjected to a pharmacophore based virtual screening against 45,257,086 molecules having 223,460,579 conformers available in MCULE database. Pharmacophore based sc...
Source: Archives of Biochemistry and Biophysics - September 21, 2023 Category: Biochemistry Authors: Mubashir Aziz Syeda Abida Ejaz Bshra A Alsfouk Ahlam Sultan Chen Li Source Type: research
Phospholipid dependency of membrane protein insertion by the Sec translocon
Biochim Biophys Acta Biomembr. 2023 Sep 19:184232. doi: 10.1016/j.bbamem.2023.184232. Online ahead of print.ABSTRACTMembrane protein insertion into and translocation across the bacterial cytoplasmic membrane are essential processes facilitated by the Sec translocon. Membrane insertion occurs co-translationally whereby the ribosome nascent chain is targeted to the translocon via signal recognition particle and its receptor FtsY. The phospholipid dependence of membrane protein insertion has remained mostly unknown. Here we assessed in vitro the dependence of the SecA independent insertion of the mannitol permease MtlA into t...
Source: Biochimica et Biophysica Acta - September 21, 2023 Category: Biochemistry Authors: Max J den Uijl Arnold J M Driessen Source Type: research
Design and Synthesis of a Library of C2-Substituted Sulfamidoadenosines to Probe Bacterial Permeability
Bioorg Med Chem Lett. 2023 Sep 19:129486. doi: 10.1016/j.bmcl.2023.129486. Online ahead of print.ABSTRACTAntibiotic resistance is a major threat to public health, and Gram-negative bacteria pose a particular challenge due to their combination of a low permeability cell envelope and efflux pumps. Our limited understanding of the chemical rules for overcoming these barriers represents a major obstacle in antibacterial drug discovery. Several recent efforts to address this problem have involved screening compound libraries for accumulation in bacteria in order to understand the structural properties required for Gram-negative...
Source: Bioorganic and Medicinal Chemistry Letters - September 21, 2023 Category: Chemistry Authors: Shibin Zhao Julian Maceren Mia Chung Samantha Stone Raphael Gei ßen Melissa S Boby Bradley S Sherborne Derek S Tan Source Type: research
Recent findings in molecular reactions of E. coli as exposed to alkylated, nano- and ordinary chitosans
Int J Biol Macromol. 2023 Sep 19:127006. doi: 10.1016/j.ijbiomac.2023.127006. Online ahead of print.ABSTRACTThe antibacterial effects of chitosan have been widely studied, but the underlying molecular mechanisms are not fully understood. We investigated the molecular responses of Escherichia coli MG1655 cell, a model gram-negative bacterium, upon exposure to chitosan (Cs), alkylated Cs (AlkCs), and chitosan nanoparticles (CsNPs). Nine target genes involved in relevant signaling pathways (ompF, ompC, ompA, mrcA, mrcB, mgtA, glnA, kdpA, lptA) were selected for analysis. A significant reduction in the expression of mrcA, mgtA...
Source: International Journal of Biological Macromolecules - September 21, 2023 Category: Biochemistry Authors: Vasighe Sadat Mirbagheri Alireza Alishahi Gholamreza Ahmadian Seyyed Hamidreza Hashemi Petroudi Seyed Mahdi Ojagh Gianfranco Romanazzi Source Type: research
Heterologous expression of a deacetylase and its application in L-glufosinate preparation
Bioprocess Biosyst Eng. 2023 Sep 21. doi: 10.1007/s00449-023-02925-x. Online ahead of print.ABSTRACTWith potent herbicidal activity, biocatalysis synthesis of L-glufosinate has drawn attention. In present research, NAP-Das2.3, a deacetylase capable of stereoselectively resolving N-acetyl-L-glufosinate to L-glufosinate mined from Arenimonas malthae, was heterologously expressed and characterized. In Escherichia coli, NAP-Das2.3 activity only reached 0.25 U/L due to the formation of inclusive bodies. Efficient soluble expression of NAP-Das2.3 was achieved in Pichia pastoris. In shake flask and 5 L bioreactor fermentation, NA...
Source: Bioprocess and Biosystems Engineering - September 21, 2023 Category: Biomedical Engineering Authors: Yuan-Shan Wang Mei-Hua Gong Jin-Hao Wang Jia-Cheng Yu Mei-Jing Li Ya-Ping Xue Yu-Guo Zheng Source Type: research
Tannin-Assisted Synthesis of Nanocomposites Loaded with Silver Nanoparticles and Their Multifunctional Applications
Biomacromolecules. 2023 Sep 21. doi: 10.1021/acs.biomac.3c00737. Online ahead of print.ABSTRACTNanocomposites have been widely used in many important areas due to their particular physical/chemical properties; however, just though a simple technology, endowing multiple functions into a single nanomaterial for realizing their multifunctional applications is still a challenge. Here, we report a robust method for the facile synthesis of Ag-based multifunctional nanocomposites via using tannin-coated phenol-formaldehyde resin nanospheres (TA-PFRN) as silver nanoparticle (Ag NP) carriers. The thickness of the tannin coating is ...
Source: Biomacromolecules - September 21, 2023 Category: Biochemistry Authors: Mengyao Xia Weikun Jiang Chen Wu Chao Wang Chang Geun Yoo Yu Liu Gaojin Lyu Source Type: research
Pentavalent outer membrane vesicles immunized mice sera confers passive protection against five prevalent pathotypes of diarrhoeagenic Escherichia coli in neonatal mice
Immunol Lett. 2023 Sep 19:S0165-2478(23)00158-X. doi: 10.1016/j.imlet.2023.09.009. Online ahead of print.ABSTRACTDiarrhoeagenic Escherichia coli (DEC) pathotypes are one of the major causative agents of diarrhoea induced childhood morbidity and mortality in developing countries. Licensed vaccines providing broad spectrum protection against DEC mediated infections are not available. Outer membrane vesicles (OMVs) are microvesicles released by gram-negative bacteria during the growth phase and contain multiple immunogenic proteins. Based on prevalence of infections, we have formulated a pentavalent outer-membrane vesicles (P...
Source: Immunology Letters - September 21, 2023 Category: Allergy & Immunology Authors: Soumalya Banerjee Prolay Halder Sanjib Das Suhrid Maiti Ushasi Bhaumik Moumita Dutta Goutam Chowdhury Kei Kitahara Shin-Ichi Miyoshi Asish Kumar Mukhopadhyay Shanta Dutta Hemanta Koley Source Type: research
Acetylation of K188 and K192 inhibits the DNA-binding ability of NarL to regulate < em > Salmonella < /em > virulence
In this study, we demonstrate that the DNA-binding sites K188 and 192 of NarL can be acetylated by bacterial metabolite acetyl phosphate and that the degree of acetylation has a considerable influence on the regulatory function of NarL. Specifically, acetylation of NarL negatively regulates the transcription of narG, narK, and napF, which affects the utilization of nitrate in Salmonella. Besides, both cell and mouse models show that acetylated K188 and K192 result in attenuated replication in RAW 264.7 cells, as well as impaired virulence in mouse model. Together, this research identifies a novel NarL acetylation mechanism...
Source: Applied and Environmental Microbiology - September 21, 2023 Category: Microbiology Authors: Liu-Qing Zhang Yi-Lin Shen Bang-Ce Ye Ying Zhou Source Type: research
DNAzyme-powered AuNRs-DNA walker for Visual Detection of Escherichia coli
New J. Chem., 2023, Accepted Manuscript DOI: 10.1039/D3NJ02386A, PaperZongqiang Li, Jingjing Li, Xu Wang, Qianru Li, Jingru Zhu, Yuying Yao, Zhiqiang Guo, Yu Wang, Su Liu, Jiadong Huang Escherichia coli (E. coli) is a common Gram-negative bacterium that is widely distributed in nature and is susceptible to natural and random genetic factors. It is a pathogenic factor for... The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - New J. Chem. latest articles - September 20, 2023 Category: Chemistry Authors: Zongqiang Li Source Type: research
Potential bacteriophages to overcome bacterial infection of Alcaligenes faecalis in diabetic ulcer
CONCLUSIONS: The 6 isolates that were isolated had the ability to infect by forming a prophage that could inhibit the growth of Alcaligenes faecalis and other pathogenic bacteria in diabetic ulcers.PMID:37728456 | DOI:10.5114/pedm.2023.125363
Source: Pediatric Endocrinology, Diabetes, and Metabolism - September 20, 2023 Category: Endocrinology Authors: Erlia Narulita Vivi Indah Nur Cahyati Riska A Febrianti Mochammad Iqbal Source Type: research
Cypate-loaded hollow mesoporous Prussian blue nanoparticle/hydrogel system for efficient photodynamic therapy/photothermal therapy dual-modal antibacterial therapy
This study provides ideas for the design of an antibiotic-free antimicrobial strategy against infectious diseases.PMID:37728144 | DOI:10.1002/jbm.a.37613
Source: Biomed Res - September 20, 2023 Category: Research Authors: Zheng Xing Bingyu Dong Xiaoxiao Zhang Lin Qiu Pengju Jiang Yang Xuan Xinye Ni Hongbin Xu Jianhao Wang Source Type: research
Cytocompatibility, antibacterial, and corrosion properties of chitosan/polymethacrylates and chitosan/poly(4-vinylpyridine) smart coatings, electrophoretically deposited on nanosilver-decorated titania nanotubes
This study involved different surface treatments of titanium surface, mainly by electrochemical oxidation to produce a nanotubular oxide layer (TNTs), a subsequent electrochemical reduction of silver nitrate and decoration of a nanotubular surface with silver nanoparticles (AgNPs), and finally electrophoretic deposition (EPD) of a composite of chitosan (CS) and either polymethacrylate-based copolymer Eudragit E 100 (EE100) or poly(4-vinylpyridine) (P4VP) coating. The effects of each stage of this multi-step modification were examined in terms of morphology, roughness, wettability, corrosion resistance, coating-substrate ad...
Source: Biomed Res - September 20, 2023 Category: Research Authors: Łukasz Pawłowski Micha ł Bartmański Anna Ronowska Adrianna Banach-Kope ć Szymon Mania Bart łomiej Michał Cieślik Aleksandra Mielewczyk-Gry ń Jakub Karczewski Andrzej Zieli ński Source Type: research
Virulence Factors and Antimicrobial Resistance of Uropathogenic < em > Escherichia coli < /em > EQ101 UPEC Isolated from UTI Patient in Quetta, Balochistan, Pakistan
Biomed Res Int. 2023 Sep 11;2023:7278070. doi: 10.1155/2023/7278070. eCollection 2023.ABSTRACTInfectious diseases have been tremendously increasing as the organisms of even normal flora become opportunistic and cause an infection, and Escherichia coli (E. coli EQ101) is one of them. Urinary tract infections are caused by various microorganisms, but Escherichia coli is the primary cause of almost 70%-90% of all UTIs. It has multiple strains, possessing diverse virulence factors, contributing to its pathogenicity. Furthermore, these virulent strains also can cause overlapping pathogenesis by sharing resistance and virulence ...
Source: Biomed Res - September 20, 2023 Category: Research Authors: Sareen Fatima Ali Akbar Muhammad Irfan Muhammad Shafee Amjad Ali Zaara Ishaq Syed Kashif Raza Abdul Samad Mohammad Y Alshahrani Syed Shah Hassan Source Type: research
