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Vaccination: Influenza Vaccine
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Total 6499 results found since Jan 2013.
Identification of catalytically active domain epitopes in neuraminidase protein of H9N2 subtype of avian influenza virus
In this study, a truncated NA gene (205-900 bp) was cloned from the NA sequence of H9N2 strain, and then expressed using pET-28a (+) vector. This purified recombinant NA protein was used to immunize BALB/c mice, and the monoclonal antibodies were screened through the indirect enzyme-linked immunosorbent assay (ELISA). Next, eight prokaryotic expression vectors were constructed for epitope identification. After cell fusion, three hybridoma cell lines producing the antibodies special to NA protein were screened by ELISA, western blotting, and indirect immunofluorescence; these were named 1B10, 2B6, and 5B2, respectively. Epi...
Source: Avian Pathology - August 15, 2023 Category: Pathology Authors: Xiangyu Huang Yiqin Cai Guihu Yin Zili Chen Jianing Hu Zichen Gao Xinyu Guo Fuqiang Xiong Xiuli Feng Source Type: research
Effects of COVID-19 and Influenza Vaccination on Rheumatic Diseases: Results From a Survey of Patient-Reported Outcomes After Vaccination
CONCLUSION: The results of the survey of patients with ARD revealed that patient-reported AEs and underlying disease flares after receiving the COVID-19 vaccine were significantly higher than those after the influenza vaccine.PMID:37582497 | PMC:PMC10427213 | DOI:10.3346/jkms.2023.38.e247
Source: J Korean Med Sci - August 15, 2023 Category: General Medicine Authors: Ji-Won Kim Ju-Yang Jung Chang-Hee Suh Young-Min Ye Hyoun-Ah Kim Source Type: research
Identification of catalytically active domain epitopes in neuraminidase protein of H9N2 subtype of avian influenza virus
In this study, a truncated NA gene (205-900 bp) was cloned from the NA sequence of H9N2 strain, and then expressed using pET-28a (+) vector. This purified recombinant NA protein was used to immunize BALB/c mice, and the monoclonal antibodies were screened through the indirect enzyme-linked immunosorbent assay (ELISA). Next, eight prokaryotic expression vectors were constructed for epitope identification. After cell fusion, three hybridoma cell lines producing the antibodies special to NA protein were screened by ELISA, western blotting, and indirect immunofluorescence; these were named 1B10, 2B6, and 5B2, respectively. Epi...
Source: Avian Pathology - August 15, 2023 Category: Pathology Authors: Xiangyu Huang Yiqin Cai Guihu Yin Zili Chen Jianing Hu Zichen Gao Xinyu Guo Fuqiang Xiong Xiuli Feng Source Type: research
Effects of COVID-19 and Influenza Vaccination on Rheumatic Diseases: Results From a Survey of Patient-Reported Outcomes After Vaccination
CONCLUSION: The results of the survey of patients with ARD revealed that patient-reported AEs and underlying disease flares after receiving the COVID-19 vaccine were significantly higher than those after the influenza vaccine.PMID:37582497 | PMC:PMC10427213 | DOI:10.3346/jkms.2023.38.e247
Source: J Korean Med Sci - August 15, 2023 Category: General Medicine Authors: Ji-Won Kim Ju-Yang Jung Chang-Hee Suh Young-Min Ye Hyoun-Ah Kim Source Type: research
Identification of catalytically active domain epitopes in neuraminidase protein of H9N2 subtype of avian influenza virus
In this study, a truncated NA gene (205-900 bp) was cloned from the NA sequence of H9N2 strain, and then expressed using pET-28a (+) vector. This purified recombinant NA protein was used to immunize BALB/c mice, and the monoclonal antibodies were screened through the indirect enzyme-linked immunosorbent assay (ELISA). Next, eight prokaryotic expression vectors were constructed for epitope identification. After cell fusion, three hybridoma cell lines producing the antibodies special to NA protein were screened by ELISA, western blotting, and indirect immunofluorescence; these were named 1B10, 2B6, and 5B2, respectively. Epi...
Source: Avian Pathology - August 15, 2023 Category: Pathology Authors: Xiangyu Huang Yiqin Cai Guihu Yin Zili Chen Jianing Hu Zichen Gao Xinyu Guo Fuqiang Xiong Xiuli Feng Source Type: research
Effects of COVID-19 and Influenza Vaccination on Rheumatic Diseases: Results From a Survey of Patient-Reported Outcomes After Vaccination
CONCLUSION: The results of the survey of patients with ARD revealed that patient-reported AEs and underlying disease flares after receiving the COVID-19 vaccine were significantly higher than those after the influenza vaccine.PMID:37582497 | DOI:10.3346/jkms.2023.38.e247
Source: J Korean Med Sci - August 15, 2023 Category: General Medicine Authors: Ji-Won Kim Ju-Yang Jung Chang-Hee Suh Young-Min Ye Hyoun-Ah Kim Source Type: research
An immune-enhanced multivalent DNA nanovaccine to prevent H7 and H9 avian influenza virus in mice
Int J Biol Macromol. 2023 Aug 12:126286. doi: 10.1016/j.ijbiomac.2023.126286. Online ahead of print.ABSTRACTH7 avian influenza virus has caused multiple human infections and poses a severe public health threat. In response to the highly variable nature of AIVs, a novel, easily regenerated DNA vaccine has great potential in treating or preventing avian influenza pandemics. Nevertheless, DNA vaccines have many disadvantages, such as weak immunogenicity and poor in vivo delivery. To further characterize and solve these issues and develop a novel H7 AIV DNA vaccine with enhanced stability and immunogenicity, we constructed nin...
Source: International Journal of Biological Macromolecules - August 14, 2023 Category: Biochemistry Authors: Shangen Xu Hailing Lan Qiaoyang Teng Xuesong Li Zheng Jin Yang Qu Jiawei Li Qihong Zhang Hong Kang Tan Hui Yin Zejun Li Kai Zhao Source Type: research
Parental acceptability of vaccinating young children against influenza and COVID-19
CONCLUSION: About 40 % of the parents were supportive of children's vaccinations against COVID-19 and influenza respectively (37 % and 42 %) in order to protect the vulnerable people around them. The media influences the decision to vaccinate especially when the level of education is low.PMID:37580209 | DOI:10.1016/j.vaccine.2023.08.005
Source: Vaccine - August 14, 2023 Category: Allergy & Immunology Authors: C éline Berthélémy Pascal Bouch é Zohra Lamiral Jean-Marc Boivin Source Type: research
CDC20 is a potential target gene to inhibit the tumorigenesis of MDCK cells
Biologicals. 2023 Aug 12;83:101697. doi: 10.1016/j.biologicals.2023.101697. Online ahead of print.ABSTRACTMDCK is currently the main cell line used for influenza vaccine production in culture. Previous studies have reported that MDCK cells possess tumorigenic ability in nude mice. Although complete cell lysis can be ensured during vaccine production, host cell DNA released after cell lysis may still pose a risk for tumorigenesis. Greater caution is needed in the production of human vaccines; therefore, the use of gene editing to establish cells incapable of forming tumors may significantly improve the safety of influenza v...
Source: Biologicals : Journal of the International Association of Biological Standardization - August 14, 2023 Category: Biotechnology Authors: Zhenbin Liu Mengyuan Pei Geng Liu Zhenyu Qiu Siya Wang Zilin Qiao Jiamin Wang Dongwu Jin Jiayou Zhang Kai Duan Xuanxuan Nian Zhongren Ma Xiaoming Yang Source Type: research
