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Procedure: Transplants

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Total 526 results found since Jan 2013.

Novel nanosome delivery system combined with siRNA targeting the antimicrobial gene DFB4: A new approach for psoriasis management?
This study encourages the exploration of topical gene silencing strategies in dermatology, and refocuses our attention on both, the role of hBD2 in psoriasis pathogenesis, and the thorny question which animal model reflects human psoriasis most faithfully. This article is protected by copyright. All rights reserved.
Source: Experimental Dermatology - March 28, 2014 Category: Dermatology Authors: A Keren, M David, A Gilhar Tags: Commentary from the Editorial Board Source Type: research

siRNA Mediated Knockdown of Tissue Factor Expression in Pigs for Xenotransplantation
Abstract Acute vascular rejection (AVR), in particular microvascular thrombosis, is an important barrier to successful pig‐to‐primate xenotransplantation. Here, we report the generation of pigs with decreased tissue factor (TF) levels induced by small interfering (si)RNA‐mediated gene silencing. Porcine fibroblasts were transfected with TF‐targeting small hairpin (sh)RNA and used for somatic cell nuclear transfer. Offspring were analyzed for siRNA, TF mRNA and TF protein level. Functionality of TF downregulation was investigated by a whole blood clotting test and a flow chamber assay. TF siRNA was expressed in all ...
Source: American Journal of Transplantation - March 23, 2015 Category: Transplant Surgery Authors: H. E. Ahrens, B. Petersen, D. Herrmann, A. Lucas‐Hahn, P. Hassel, M. Ziegler, W. A. Kues, U. Baulain, W. Baars, R. Schwinzer, J. Denner, D. Rataj, S. Werwitzke, A. Tiede, A. K. Bongoni, P. S. Garimella, A. Despont, R. Rieben, H. Niemann Tags: Brief Communication Source Type: research

Vitamin A-coupled liposomes containing siRNA against HSP47 ameliorate skin fibrosis in chronic graft-versus-host disease
Chronic graft-versus-host disease (GVHD) after allogeneic hematopoietic stem cell transplantation (SCT) is characterized by multiorgan fibrosis and profoundly affects the quality of life of transplant survivors. Heat shock protein 47 (HSP47), a collagen-specific molecular chaperone, plays a critical role in collagen synthesis in myofibroblasts. We explored the role of HSP47 in the fibrotic process of cutaneous chronic GVHD in mice. Immunohistochemical analysis showed massive fibrosis with elevated amounts of collagen deposits and accumulation of F4/80+ macrophages, as well as myofibroblasts expressing HSP47 and retinol-bin...
Source: Blood - March 29, 2018 Category: Hematology Authors: Yamakawa, T., Ohigashi, H., Hashimoto, D., Hayase, E., Takahashi, S., Miyazaki, M., Minomi, K., Onozawa, M., Niitsu, Y., Teshima, T. Tags: Transplantation Source Type: research

AMF siRNA treatment of keloid through inhibition signaling pathway of RhoA/ROCK1
In conclusion, AMF siRNA is expected to be a novel strategy to treat KD by inhibiting signaling pathway of RhoA/ROCK1.
Source: Genes and Diseases - May 19, 2018 Category: Genetics & Stem Cells Source Type: research

Ocular Instillation of Vitamin A-Coupled Liposomes Containing HSP47 siRNA Ameliorates Dry Eye Syndrome in Chronic Graft-Versus-Host Disease
Chronic graft-versus-host disease (GVHD) is characterized by multiorgan fibrosis and affects the quality of life of transplant survivors. Ocular manifestations are found in 40-60% of GVHD patients, and the most common symptom is dry eye syndrome associated with lacrimal gland fibrosis. We previously demonstrated heat shock protein 47 (HSP47), a collagen specific molecular chaperone, plays a critical role in excessive collagen synthesis in chronic GVHD, and systemic administration of vitamin A-coupled liposomes carrying HSP47 siRNA (VA-lip HSP47) ameliorates skin and salivary gland fibrosis in chronic GVHD (Yamakawa, et al, Blood 2018).
Source: Biology of Blood and Marrow Transplantation - January 31, 2019 Category: Hematology Authors: Hiroyuki Ohigashi, Daigo Hashimoto, Shuichiro Takahashi, Tomohiro Yamakawa, Eiko Hayase, Takanori Teshima Tags: 229 Source Type: research

Novel Complement C5 Small-interfering RNA Lipid Nanoparticle Prolongs Graft Survival in a Hypersensitized Rat Kidney Transplant Model
Conclusions. C5 siRNA-LNP combined with immunosuppressants CsA and DSG downregulated C5 activity and significantly prolonged graft survival compared with control siRNA-LNP with CsA and DSG. Downregulation of C5 expression using C5 siRNA-LNP may be an effective therapeutic approach for AMR.
Source: Transplantation - December 1, 2022 Category: Transplant Surgery Tags: Original Basic Science Source Type: research

Improved islet transplantation outcome by the co-delivery of siRNAs for iNOS and 17β-estradiol using an R3V6 peptide carrier.
Abstract Silencing target genes such as inducible nitric oxide synthase (iNOS) using small interfering double-stranded RNA (siRNA) in islet cells has been attempted to enhance the survival of transplanted islets. However, the efficient and safe delivery of siRNA into intact islets is challenging. Here, we prepared R3V6 peptides containing a three-arginine stretch and a six-valine stretch, which form micelles with hydrophobic valine cores and cationic arginine surfaces in aqueous solution, to co-deliver siRNA and cytoprotective hydrophobic drugs to islet cells. The cationic surfaces bound to the negatively charged ...
Source: Biomaterials - December 6, 2014 Category: Materials Science Authors: Hwang HJ, Lee M, Park JH, Jung HS, Kang JG, Kim CS, Lee SJ, Ihm SH Tags: Biomaterials Source Type: research

Naked caspase 3 small interfering RNA is effective in cold preservation but not in autotransplantation of porcine kidneys
Conclusions: Naked caspase 3 siRNA administered into the kidney was effective in cold preservation but not enough to protect posttransplant kidneys, which might be because of systemic complementary responses overcoming local effects.
Source: Journal of Surgical Research - July 27, 2012 Category: Surgery Authors: Cheng Yang, Yichen Jia, Tian Zhao, Yinjia Xue, Zitong Zhao, Junlin Zhang, Jina Wang, Xuanchuan Wang, Yongyin Qiu, Miao Lin, Dong Zhu, Guisheng Qi, Yue Qiu, Qunye Tang, Ruiming Rong, Ming Xu, Sujie Ni, Bin Lai, Michael L. Nicholson, Tongyu Zhu, Bin Yang Tags: Transplantation/Immunology Source Type: research

Synergic silencing of costimulatory molecules prevents cardiac allograft rejection
Conclusion: This study demonstrates that the simultaneous silencing of CD40 and CD80 genes has synergistic effects in preventing allograft rejection, and may therefore have therapeutic potential in clinical transplantation.
Source: Journal of Translational Medicine - May 22, 2014 Category: Research Authors: Xusheng ZhangYanling LiuGuangfeng ZhangJun ShiXiao ZhangXiufen ZhengAlex JiangZhu-Xu ZhangNathan JohnstonKing SiuRuiqi ChenDameng LianDavid KoosDouglas QuanWei-Ping Min Source Type: research

Suppression of NF‐kappaB p65 expression attenuates delayed xenograft rejection
ConclusionTransfection of NF‐kappaB p65 siRNA in donor animals can delay the emergence of DXR. This treatment may be used as part of strategies to minimize the complex and multi‐faceted rejection responses in vascularized xenografts.
Source: Xenotransplantation - March 13, 2013 Category: Transplant Surgery Authors: Zhenya Shen, Wenxue Ye, Xiaomei Ten Tags: Original Article Source Type: research

Small Interfering RNA Targeting Nuclear Factor Kappa B to Prevent Vein Graft Stenosis in Rat Models
We examined whether neointimal hyperplasia and vein graft stenosis could be inhibited by silencing the NF-κB gene with small interference RNA (siRNA).Methods: Sixty adult male Sprague-Dawley rats were randomly divided into a normal vein group, a vein graft group, a scrambled siRNA group, and an NF-κB siRNA group. We performed reverse interpositional grafting of the autologous external jugular vein to the abdominal aorta. Vein grafts were treated with liposome and gel complexes containing NF-κB siRNA or scrambled siRNA. The levels of monocyte chemoattractant protein -1, tumor necrosis factor-α, and NF-κB p65 in vessel ...
Source: Transplantation Proceedings - July 1, 2013 Category: Transplant Surgery Authors: X.B. Meng, X.L. Bi, H.L. Zhao, J.B. Feng, J.P. Zhang, G.M. Song, W.Y. Sun, Y.W. Bi Tags: Experimental Observations Source Type: research

PU.1-silenced dendritic cells prolong allograft survival in rats receiving intestinal transplantation.
CONCLUSION: PU.1-silenced semi-mature DCs induced partial immune tolerance both in vitro and in vivo, which could be used as a new strategy to promote transplantation tolerance. PMID: 24282365 [PubMed - in process]
Source: World Journal of Gastroenterology : WJG - November 21, 2013 Category: Gastroenterology Authors: Xu XW, Ding BW, Zhu CR, Ji W, Li JS Tags: World J Gastroenterol Source Type: research

Silencing ephrinB3 improves functional recovery following spinal cord injury.
Abstract EphrinB3 may restrict axonal regeneration and recovery following traumatic injury of the adult mammalian central nervous system (CNS). Therefore, inhibition of ephrinB3 expression may enhance the ability of the nervous system to regenerate following damage. In the present study, lentiviral expressing vectors, pGCSIL-GFP, expressing an active small interfering RNA (siRNA) targeting the EphB3 sequence, were used to determine the effect of inhibiting EphB3 on nerve functional recovery and regeneration. Basso-Beattie-Bresnahan (BBB) locomotor scores and growth-associated protein, 43 kDa (GAP43) expression le...
Source: Molecular Medicine - March 6, 2014 Category: Molecular Biology Authors: Qu Y, Zhao J, Wang Y, Gao Z Tags: Mol Med Rep Source Type: research