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Infectious Disease: Adenoviruses

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Total 599 results found since Jan 2013.

Genetic Modification of Dendritic Cells with RNAi
Gene silencing with RNAi is an invaluable technique in cell biology to knock down the target gene expression. Dendritic cells (DC) are the most effective antigen-presenting cells (APC), and the efficacy of antigen presentation is tightly controlled by the stimulatory as well as inhibitory mechanisms. In recent studies, RNAi technology has been employed to silence the expression of the intrinsic inhibitors of antigen presentation in DC, improving the efficacy of DC vacciness against tumor antigens in pre-clinical studies. Here, we describe the technique of using siRNA oligonucleotides, adenovirus expressing shRNA (Ad-shRNA)...
Source: Springer protocols feed by Cancer Research - April 7, 2014 Category: Cancer & Oncology Source Type: news

Silencing histone deacetylase 2 using small hairpin RNA induces regression of fibrotic plaque in a rat model of Peyronie's disease
Conclusion• Specific inhibition of HDAC2 with RNA interference may represent a novel targeted therapy for PD.
Source: BJU International - May 19, 2014 Category: Urology & Nephrology Authors: Ki‐Dong Kwon, Min Ji Choi, Jin‐Mi Park, Kang‐Moon Song, Mi‐Hye Kwon, Dulguun Batbold, Guo Nan Yin, Woo Jean Kim, Ji‐Kan Ryu, Jun‐Kyu Suh Tags: Translational Science Source Type: research

Orphan nuclear receptor Nur77 mediates fasting-induced hepatic fibroblast growth factor 21 expression.
In conclusion, this study shows that Nur77 mediates fasting-induced hepatic FGF21 expression, and suggests an alternative mechanism via which hepatic FGF21 transcription is mediated under fasting conditions. PMID: 24885573 [PubMed - as supplied by publisher]
Source: Endocrinology - June 2, 2014 Category: Endocrinology Authors: Min AK, Bae KH, Jung YA, Choi YK, Kim MJ, Kim JH, Jeon JH, Kim JG, Lee IK, Park KG Tags: Endocrinology Source Type: research

Promotion of Lung Tumor Growth by Interleukin-17.
Abstract Recent findings demonstrate that inhaled cigarette smoke, the predominant lung carcinogen, elicits a T helper 17 (Th17) inflammatory phenotype. Interleukin-17A (IL-17), the hallmark cytokine of Th17 inflammation, displays pro- and anti-tumorigenic properties in a manner that varies according to tumor type and assay system. To investigate the role of IL-17 in lung tumor growth, we used an autochthonous tumor model (K-ras(LA1) mice) with lung delivery of a recombinant adenovirus that expresses IL-17A. Virus-mediated expression of IL-17A in K-Ras(LA1) mice at 8-10 weeks of age doubled lung tumor growth in th...
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - July 18, 2014 Category: Cytology Authors: Xu B, Guenther JF, Pociask DA, Wang Y, Kolls JK, You Z, Chandrasekar B, Shan B, Sullivan DE, Morris GF Tags: Am J Physiol Lung Cell Mol Physiol Source Type: research

Blockade of the OX40/OX40L pathway and induction of PD-L1 synergistically protects mouse islet allografts from rejection.
CONCLUSIONS: This study demonstrates the successful construction of the recombinant lentivirus vector OX40L-RNAi-LV and adenovirus vector Ad-PD-L1 for the blockade of OX40/OX40L and activation of PD-1/PD-L1 costimulatory pathways simultaneously in pancreatic islet allografts in diabetic mice. Combination therapy with these two vectors resulted in inhibition of T cell activation, synergistically prolonging the survival time of pancreatic islet allografts. PMID: 25043090 [PubMed - in process]
Source: Chinese Medical Journal - July 1, 2014 Category: Journals (General) Authors: Li T, Ma R, Zhu J, Wang F, Huang L, Leng X Tags: Chin Med J (Engl) Source Type: research

The Role of Caveolin-1 and Syndecan-4 in the Internalization of PEGylated PAMAM Dendrimer Polyplexes into Myoblast and Hepatic Cells.
This study gives a clue how to take strategies by up- or down-regulation of the expressions of Syndecan-4 and Caveolin-1 to improve in vivo gene delivery efficiency of the PEG-PAMAM dendrimers in clinical transgenic therapy. PMID: 25083608 [PubMed - as supplied by publisher]
Source: European Journal of Pharmaceutics and Biopharmaceutics - July 29, 2014 Category: Drugs & Pharmacology Authors: Shen W, van Dongen MA, Han Y, Yu M, Li Y, Liu G, BanaszakHoll MM, Qi R Tags: Eur J Pharm Biopharm Source Type: research

Sumo E2 Ligase UBC9 is Required for Efficient Protein Quality Control in Cardiomyocytes.
Conclusions: UBC9 plays a significant role in cardiomyocyte PQC and its activity can be exploited to reduce toxic levels of misfolded or aggregated proteins in cardiomyopathy. PMID: 25097219 [PubMed - as supplied by publisher]
Source: Circulation Research - August 5, 2014 Category: Cardiology Authors: Gupta MK, Gulick J, Liu R, Wang X, Molkentin JD, Robbins J Tags: Circ Res Source Type: research

SUMOylation protects against IL-1β-induced apoptosis in INS-1 832/13 and human islets.
We examined insulin secretion, intracellular Ca(2+) responses, induction of ER stress markers and inducible nitric oxide synthase (iNOS) expression, and apoptosis by TUNEL and caspase 3 cleavage. Surprisingly, up-regulation of SENP1 reduces insulin secretion and impairs intracellular Ca(2+) handling. This secretory dysfunction is due to SENP1-induced cell death. Indeed, the detrimental effect of SENP1 on secretory function is diminished when two mediators of β-cell death, iNOS and NFκB, are pharmacologically inhibited. Conversely, enhanced SUMOylation protects against IL-1β-induced cell death. This is associated with re...
Source: American Journal of Physiology. Endocrinology and Metabolism - August 19, 2014 Category: Physiology Authors: Hajmrle C, Ferdaoussi M, Plummer G, Spigelman AF, Lai K, Fox JE, MacDonald PE Tags: Am J Physiol Endocrinol Metab Source Type: research

Shp/Npas2 axis in regulating the oscillation of liver lipid metabolism
Conclusion: Shp and Npas2 crosstalk is essential to maintain hepatic lipid homeostasis. (Hepatology 2014)
Source: Hepatology - September 12, 2014 Category: Internal Medicine Authors: Sang Min Lee, Yuxia Zhang, Hiroyuki Tsuchiya, Rana Smalling, Anton M. Jetten, Li Wang Tags: Steatohepatitis and Metabolic Liver Disease Source Type: research

Promotion of lung tumor growth by interleukin-17
Recent findings demonstrate that inhaled cigarette smoke, the predominant lung carcinogen, elicits a T helper 17 (Th17) inflammatory phenotype. Interleukin-17A (IL-17), the hallmark cytokine of Th17 inflammation, displays pro- and antitumorigenic properties in a manner that varies according to tumor type and assay system. To investigate the role of IL-17 in lung tumor growth, we used an autochthonous tumor model (K-RasLA1 mice) with lung delivery of a recombinant adenovirus that expresses IL-17A. Virus-mediated expression of IL-17A in K-RasLA1 mice at 8–10 wk of age doubled lung tumor growth in 3 wk relative to litte...
Source: AJP: Lung Cellular and Molecular Physiology - September 15, 2014 Category: Respiratory Medicine Authors: Xu, B., Guenther, J. F., Pociask, D. A., Wang, Y., Kolls, J. K., You, Z., Chandrasekar, B., Shan, B., Sullivan, D. E., Morris, G. F. Tags: ARTICLES Source Type: research

ASPP2 attenuates triglycerides to protect against hepatocyte injury by reducing autophagy in a cell and mouse model of non‐alcoholic fatty liver disease
In conclusion, ASPP2 may participate in the lipid metabolism of non‐alcoholic steatohepatitis and attenuate liver failure.
Source: Journal of Cellular and Molecular Medicine - September 25, 2014 Category: Molecular Biology Authors: Fang Xie, Lin Jia, Minghua Lin, Ying Shi, Jiming Yin, Yin Liu, Dexi Chen, Qinghua Meng Tags: Original Article Source Type: research

ASPP2 attenuates triglycerides to protect against hepatocyte injury by reducing autophagy in a cell and mouse model of non-alcoholic fatty liver disease.
In conclusion, ASPP2 may participate in the lipid metabolism of non-alcoholic steatohepatitis and attenuate liver failure. PMID: 25256142 [PubMed - as supplied by publisher]
Source: J Cell Mol Med - September 25, 2014 Category: Molecular Biology Authors: Xie F, Jia L, Lin M, Shi Y, Yin J, Liu Y, Chen D, Meng Q Tags: J Cell Mol Med Source Type: research

SUMOylation protects against IL-1{beta}-induced apoptosis in INS-1 832/13 cells and human islets
We examined insulin secretion, intracellular Ca2+ responses, induction of endoplasmic reticulum stress markers and inducible nitric oxide synthase (iNOS) expression, and apoptosis by TUNEL and caspase 3 cleavage. Surprisingly, upregulation of SENP1 reduces insulin secretion and impairs intracellular Ca2+ handling. This secretory dysfunction is due to SENP1-induced cell death. Indeed, the detrimental effect of SENP1 on secretory function is diminished when two mediators of β-cell death, iNOS and NF-B, are pharmacologically inhibited. Conversely, enhanced SUMOylation protects against IL-1β-induced cell death. This ...
Source: AJP: Endocrinology and Metabolism - October 15, 2014 Category: Endocrinology Authors: Hajmrle, C., Ferdaoussi, M., Plummer, G., Spigelman, A. F., Lai, K., Manning Fox, J. E., MacDonald, P. E. Tags: Articles Source Type: research

miR-134 Regulates Ischemia/Reperfusion Injury-Induced Neuronal Cell Death by Regulating CREB Signaling
Abstract microRNA-134 (miR-134) has been reported to be a brain-specific miRNA and is differently expressed in brain tissues subjected to ischemic injury. However, the underlying mechanism of miR-134 in regulating cerebral ischemic injury remains poorly understood. The current study was designed to delineate the molecular basis of miR-134 in regulating cerebral ischemic injury. Using the oxygen-glucose deprivation (OGD) model of hippocampal neuron ischemia in vitro, we found that the overexpression of miR-134 mediated by recombinant adeno-associated virus (AAV) vector infection significantly promoted neuron death ...
Source: Journal of Molecular Neuroscience - October 16, 2014 Category: Neuroscience Source Type: research

Role of the Prostaglandin E2/E-Prostanoid 2 Receptor Signaling Pathway in TGF-β-induced Mice Mesangial Cell damage.
Abstract The prostaglandin E2 receptor, E-prostanoid 2 (EP2), plays important roles in mice glomerular mesangial cell (MCs) damage induced by transforming growth factor-β1(TGF β1), however the molecular mechanisms for this remain unknown. The present study examined the role of the EP2 signaling pathway in TGF-β1-induced MCs proliferation, extracellular matrix (ECM) accumulation and expression of prostaglandin E2 synthase (PGES). We generated primary mice MCs. Results showed MCs proliferation promoted by TGF-β1 were increased, however, the production of Cyclic Adenosine 3',5'-Monophosphate (cAMP) and prostaglan...
Source: Bioscience Reports - October 20, 2014 Category: Biomedical Science Authors: Li NN, Xu YY, Chen XL, Fan YP, Wu JH Tags: Biosci Rep Source Type: research