Lessons from resistance analysis in clinical trials of IV zanamivir
In this study ten resistance substitutions, two of which were treatment emergent, were detected by NGS that otherwise would not have been detectable by Sanger sequencing. None of the substitutions were present at any other timepoints analysed. The effect these mutations have on clinical response is difficult to characterize; in fact, all patients from which these variants were isolated had a successful clinical outcome and the effect on clinical response was therefore likely minimal. Although NGS is becoming a routine method for nucleic acid sequencing and will detect substitutions previously undetected by Sanger sequencin...
Source: Virus Research - January 7, 2023 Category: Virology Authors: Phillip J Yates Nalini Mehta Helen A Watson Amanda F Peppercorn Source Type: research
Lessons from resistance analysis in clinical trials of IV zanamivir
In this study ten resistance substitutions, two of which were treatment emergent, were detected by NGS that otherwise would not have been detectable by Sanger sequencing. None of the substitutions were present at any other timepoints analysed. The effect these mutations have on clinical response is difficult to characterize; in fact, all patients from which these variants were isolated had a successful clinical outcome and the effect on clinical response was therefore likely minimal. Although NGS is becoming a routine method for nucleic acid sequencing and will detect substitutions previously undetected by Sanger sequencin...
Source: Virus Research - January 7, 2023 Category: Virology Authors: Phillip J Yates Nalini Mehta Helen A Watson Amanda F Peppercorn Source Type: research
Lessons from resistance analysis in clinical trials of IV zanamivir
In this study ten resistance substitutions, two of which were treatment emergent, were detected by NGS that otherwise would not have been detectable by Sanger sequencing. None of the substitutions were present at any other timepoints analysed. The effect these mutations have on clinical response is difficult to characterize; in fact, all patients from which these variants were isolated had a successful clinical outcome and the effect on clinical response was therefore likely minimal. Although NGS is becoming a routine method for nucleic acid sequencing and will detect substitutions previously undetected by Sanger sequencin...
Source: Virus Research - January 7, 2023 Category: Virology Authors: Phillip J Yates Nalini Mehta Helen A Watson Amanda F Peppercorn Source Type: research
Lessons from resistance analysis in clinical trials of IV zanamivir
In this study ten resistance substitutions, two of which were treatment emergent, were detected by NGS that otherwise would not have been detectable by Sanger sequencing. None of the substitutions were present at any other timepoints analysed. The effect these mutations have on clinical response is difficult to characterize; in fact, all patients from which these variants were isolated had a successful clinical outcome and the effect on clinical response was therefore likely minimal. Although NGS is becoming a routine method for nucleic acid sequencing and will detect substitutions previously undetected by Sanger sequencin...
Source: Virus Research - January 7, 2023 Category: Virology Authors: Phillip J Yates Nalini Mehta Helen A Watson Amanda F Peppercorn Source Type: research
Molecules, Vol. 27, Pages 7797: Essential Oils and Their Compounds as Potential Anti-Influenza Agents
eji
Essential oils (EOs) are chemical substances, mostly produced by aromatic plants in response to stress, that have a history of medicinal use for many diseases. In the last few decades, EOs have continued to gain more attention because of their proven therapeutic applications against the flu and other infectious diseases. Influenza (flu) is an infectious zoonotic disease that affects the lungs and their associated organs. It is a public health problem with a huge health burden, causing a seasonal outbreak every year. Occasionally, it comes as a disease pandemic with unprecedentedly high hospitalization and mortality...
Source: Molecules - November 12, 2022 Category: Chemistry Authors: Ayodeji Oluwabunmi Oriola Adebola Omowunmi Oyedeji Tags: Review Source Type: research
Binding of Glycans to the SARS CoV-2 Spike Protein, an Open Question: NMR Data on Binding Site Localization, Affinity, and Selectivity
Chemistry. 2022 Sep 26. doi: 10.1002/chem.202202614. Online ahead of print.ABSTRACTWe have used NMR experiments to explore binding of selected glycans and glycomimetics to the SARS CoV-2 spike glycoprotein (S-protein) and to its receptor binding domain (RBD). STD NMR experiments confirm binding of sialoglycans to the S-protein of the prototypic Wuhan strain virus and yield dissociation constants in the mM range. The absence of STD effects for sialoglycans in the presence of the Omicron/BA.1 S-protein reflects a loss of binding as a result of S-protein evolution. Likewise, no STD effects are observed for the deletion mutant...
Source: Chemistry - September 26, 2022 Category: Chemistry Authors: Thorben Maass George Ssebyatika Marlene Br ückner Lea Breckwoldt Thomas Krey Alvaro Mallagaray Thomas Peters Martin Frank Robert Creutznacher Source Type: research
Molecules, Vol. 27, Pages 5920: In Silico Studies Reveal Peramivir and Zanamivir as an Optimal Drug Treatment Even If H7N9 Avian Type Influenza Virus Acquires Further Resistance
dekar
An epidemic of avian type H7N9 influenza virus, which took place in China in 2013, was enhanced by a naturally occurring R294K mutation resistant against Oseltamivir at the catalytic site of the neuraminidase. To cope with such drug-resistant neuraminidase mutations, we applied the molecular docking technique to evaluate the fitness of the available drugs such as Oseltamivir, Zanamivir, Peramivir, Laninamivir, L-Arginine and Benserazide hydrochloride concerning the N9 enzyme with single (R294K, R119K, R372K), double (R119_294K, R119_372K, R294_372K) and triple (R119_294_372K) mutations in the pocket. We found tha...
Source: Molecules - September 12, 2022 Category: Chemistry Authors: Edita Sarukhanyan Tipack Ayothyapattanam Shanmugam Thomas Dandekar Tags: Article Source Type: research
Insights into binding molecular mechanism of hemagglutinin H3N2 of influenza virus complexed with arbidol and its derivative: A molecular dynamics simulation perspective
Comput Biol Chem. 2022 Aug 28;101:107764. doi: 10.1016/j.compbiolchem.2022.107764. Online ahead of print.ABSTRACTRecently, the H3N2 influenza outbreak has caused serious global public health concern for future control of the next influenza pandemic. Since using current anti-influenza drugs targeting neuraminidase (oseltamivir and zanamivir) and the proton M2 channel (amantadine and rimantadine) leads to drug resistance, it is essential to seek new anti-viral agents that act on additional viral targets. Hemagglutinin (HA), a glycoprotein embedded in the viral surface and playing a critical role in influenza the viral replic...
Source: Computational Biology and Chemistry - September 1, 2022 Category: Bioinformatics Authors: Thitiya Boonma Nattharuja Soikudrua Bodee Nutho Thanyada Rungrotmongkol Nadtanet Nunthaboot Source Type: research
Insights into binding molecular mechanism of hemagglutinin H3N2 of influenza virus complexed with arbidol and its derivative: A molecular dynamics simulation perspective
Comput Biol Chem. 2022 Aug 28;101:107764. doi: 10.1016/j.compbiolchem.2022.107764. Online ahead of print.ABSTRACTRecently, the H3N2 influenza outbreak has caused serious global public health concern for future control of the next influenza pandemic. Since using current anti-influenza drugs targeting neuraminidase (oseltamivir and zanamivir) and the proton M2 channel (amantadine and rimantadine) leads to drug resistance, it is essential to seek new anti-viral agents that act on additional viral targets. Hemagglutinin (HA), a glycoprotein embedded in the viral surface and playing a critical role in influenza the viral replic...
Source: Computational Biology and Chemistry - September 1, 2022 Category: Bioinformatics Authors: Thitiya Boonma Nattharuja Soikudrua Bodee Nutho Thanyada Rungrotmongkol Nadtanet Nunthaboot Source Type: research
Insights into binding molecular mechanism of hemagglutinin H3N2 of influenza virus complexed with arbidol and its derivative: A molecular dynamics simulation perspective
Comput Biol Chem. 2022 Aug 28;101:107764. doi: 10.1016/j.compbiolchem.2022.107764. Online ahead of print.ABSTRACTRecently, the H3N2 influenza outbreak has caused serious global public health concern for future control of the next influenza pandemic. Since using current anti-influenza drugs targeting neuraminidase (oseltamivir and zanamivir) and the proton M2 channel (amantadine and rimantadine) leads to drug resistance, it is essential to seek new anti-viral agents that act on additional viral targets. Hemagglutinin (HA), a glycoprotein embedded in the viral surface and playing a critical role in influenza the viral replic...
Source: Computational Biology and Chemistry - September 1, 2022 Category: Bioinformatics Authors: Thitiya Boonma Nattharuja Soikudrua Bodee Nutho Thanyada Rungrotmongkol Nadtanet Nunthaboot Source Type: research
Insights into binding molecular mechanism of hemagglutinin H3N2 of influenza virus complexed with arbidol and its derivative: A molecular dynamics simulation perspective
Comput Biol Chem. 2022 Aug 28;101:107764. doi: 10.1016/j.compbiolchem.2022.107764. Online ahead of print.ABSTRACTRecently, the H3N2 influenza outbreak has caused serious global public health concern for future control of the next influenza pandemic. Since using current anti-influenza drugs targeting neuraminidase (oseltamivir and zanamivir) and the proton M2 channel (amantadine and rimantadine) leads to drug resistance, it is essential to seek new anti-viral agents that act on additional viral targets. Hemagglutinin (HA), a glycoprotein embedded in the viral surface and playing a critical role in influenza the viral replic...
Source: Computational Biology and Chemistry - September 1, 2022 Category: Bioinformatics Authors: Thitiya Boonma Nattharuja Soikudrua Bodee Nutho Thanyada Rungrotmongkol Nadtanet Nunthaboot Source Type: research
Insights into binding molecular mechanism of hemagglutinin H3N2 of influenza virus complexed with arbidol and its derivative: A molecular dynamics simulation perspective
Comput Biol Chem. 2022 Aug 28;101:107764. doi: 10.1016/j.compbiolchem.2022.107764. Online ahead of print.ABSTRACTRecently, the H3N2 influenza outbreak has caused serious global public health concern for future control of the next influenza pandemic. Since using current anti-influenza drugs targeting neuraminidase (oseltamivir and zanamivir) and the proton M2 channel (amantadine and rimantadine) leads to drug resistance, it is essential to seek new anti-viral agents that act on additional viral targets. Hemagglutinin (HA), a glycoprotein embedded in the viral surface and playing a critical role in influenza the viral replic...
Source: Computational Biology and Chemistry - September 1, 2022 Category: Bioinformatics Authors: Thitiya Boonma Nattharuja Soikudrua Bodee Nutho Thanyada Rungrotmongkol Nadtanet Nunthaboot Source Type: research
Characterization of influenza B viruses with reduced susceptibility to influenza neuraminidase inhibitors
Antiviral Res. 2022 Mar 15;200:105280. doi: 10.1016/j.antiviral.2022.105280. Online ahead of print.ABSTRACTA total of 3425 influenza B viruses collected from the Asia-Pacific region were tested against the four registered neuraminidase inhibitors (NAIs) (oseltamivir carboxylate, zanamivir, peramivir and laninamivir) as part of the routine surveillance work at the WHO Collaborating Centre for Research and Reference on Influenza, Melbourne between 2016 and 2020. Forty-five influenza B viruses with reduced susceptibility to one or more NAIs were identified. While the majority of these had neuraminidase (NA) mutations that wer...
Source: Antiviral Research - March 19, 2022 Category: Virology Authors: Sook Kwan Brown Yeu-Yang Tseng Ammar Aziz Mariana Baz Ian G Barr Source Type: research
Characterization of influenza B viruses with reduced susceptibility to influenza neuraminidase inhibitors
Antiviral Res. 2022 Mar 15;200:105280. doi: 10.1016/j.antiviral.2022.105280. Online ahead of print.ABSTRACTA total of 3425 influenza B viruses collected from the Asia-Pacific region were tested against the four registered neuraminidase inhibitors (NAIs) (oseltamivir carboxylate, zanamivir, peramivir and laninamivir) as part of the routine surveillance work at the WHO Collaborating Centre for Research and Reference on Influenza, Melbourne between 2016 and 2020. Forty-five influenza B viruses with reduced susceptibility to one or more NAIs were identified. While the majority of these had neuraminidase (NA) mutations that wer...
Source: Antiviral Research - March 19, 2022 Category: Virology Authors: Sook Kwan Brown Yeu-Yang Tseng Ammar Aziz Mariana Baz Ian G Barr Source Type: research
Characterization of influenza B viruses with reduced susceptibility to influenza neuraminidase inhibitors
Antiviral Res. 2022 Mar 15;200:105280. doi: 10.1016/j.antiviral.2022.105280. Online ahead of print.ABSTRACTA total of 3425 influenza B viruses collected from the Asia-Pacific region were tested against the four registered neuraminidase inhibitors (NAIs) (oseltamivir carboxylate, zanamivir, peramivir and laninamivir) as part of the routine surveillance work at the WHO Collaborating Centre for Research and Reference on Influenza, Melbourne between 2016 and 2020. Forty-five influenza B viruses with reduced susceptibility to one or more NAIs were identified. While the majority of these had neuraminidase (NA) mutations that wer...
Source: Antiviral Research - March 19, 2022 Category: Virology Authors: Sook Kwan Brown Yeu-Yang Tseng Ammar Aziz Mariana Baz Ian G Barr Source Type: research
