Surgically induced aortic coarctation in a neonatal porcine model allows for longitudinal assessment of cardiovascular changes
CONCLUSIONS: This model addresses many limitations of prior COA models including neonatal creation at an expected anatomic position with intimal injury and vessel sizes similar to humans.PMID:38488518 | DOI:10.1152/ajpheart.00087.2024 (Source: American Journal of Physiology. Heart and Circulatory Physiology)
Source: American Journal of Physiology. Heart and Circulatory Physiology - March 15, 2024 Category: Physiology Authors: Michael Stellon Leah Gober Matthew Allen Culver Joshua Hermsen Dana Irrer Colleen Witzenburg Alejandro Rold án-Alzate Luke Lamers Source Type: research

BAG3 localizes to mitochondria in cardiac fibroblasts and regulates mitophagy
Am J Physiol Heart Circ Physiol. 2024 Mar 15. doi: 10.1152/ajpheart.00736.2023. Online ahead of print.ABSTRACTThe co-chaperone BAG3 is a central node in protein quality control in the heart. In humans and animal models, decreased BAG3 expression is associated with cardiac dysfunction and dilated cardiomyopathy. While previous studies focused on BAG3 in cardiomyocytes, cardiac fibroblasts are also critical drivers of pathologic remodeling. Yet, BAG3's role in cardiac fibroblasts is almost completely unexplored. Here, we show BAG3 is expressed in primary rat neonatal cardiac fibroblasts and preferentially localizes to mitoch...
Source: American Journal of Physiology. Heart and Circulatory Physiology - March 15, 2024 Category: Physiology Authors: Thomas G Martin Laura A Sherer Jonathan A Kirk Source Type: research

Single-cell ionic current phenotyping explains stem cell-derived cardiomyocyte action potential morphology
In this study, we use recently published, brief (10 s), dynamic voltage clamp (VC) data to provide mechanistic insights into the ionic currents contributing to AP heterogeneity - we call this approach rapid ionic current phenotyping (RICP). Features of this VC data were correlated to AP recordings from the same cells and we used computational models to generate mechanistic insights into cellular heterogeneity. This analysis uncovered several interesting links between AP morphology and ionic current density: both L-type calcium and sodium currents contribute to upstroke velocity, rapid delayed rectifier K+ current is the ma...
Source: American Journal of Physiology. Heart and Circulatory Physiology - March 15, 2024 Category: Physiology Authors: Alexander P Clark Siyu Wei Kristin Fullerton Trine Krogh-Madsen David J Christini Source Type: research

Surgically induced aortic coarctation in a neonatal porcine model allows for longitudinal assessment of cardiovascular changes
CONCLUSIONS: This model addresses many limitations of prior COA models including neonatal creation at an expected anatomic position with intimal injury and vessel sizes similar to humans.PMID:38488518 | DOI:10.1152/ajpheart.00087.2024 (Source: American Journal of Physiology. Heart and Circulatory Physiology)
Source: American Journal of Physiology. Heart and Circulatory Physiology - March 15, 2024 Category: Physiology Authors: Michael Stellon Leah Gober Matthew Allen Culver Joshua Hermsen Dana Irrer Colleen Witzenburg Alejandro Rold án-Alzate Luke Lamers Source Type: research

BAG3 localizes to mitochondria in cardiac fibroblasts and regulates mitophagy
Am J Physiol Heart Circ Physiol. 2024 Mar 15. doi: 10.1152/ajpheart.00736.2023. Online ahead of print.ABSTRACTThe co-chaperone BAG3 is a central node in protein quality control in the heart. In humans and animal models, decreased BAG3 expression is associated with cardiac dysfunction and dilated cardiomyopathy. While previous studies focused on BAG3 in cardiomyocytes, cardiac fibroblasts are also critical drivers of pathologic remodeling. Yet, BAG3's role in cardiac fibroblasts is almost completely unexplored. Here, we show BAG3 is expressed in primary rat neonatal cardiac fibroblasts and preferentially localizes to mitoch...
Source: American Journal of Physiology. Heart and Circulatory Physiology - March 15, 2024 Category: Physiology Authors: Thomas G Martin Laura A Sherer Jonathan A Kirk Source Type: research

Single-cell ionic current phenotyping explains stem cell-derived cardiomyocyte action potential morphology
In this study, we use recently published, brief (10 s), dynamic voltage clamp (VC) data to provide mechanistic insights into the ionic currents contributing to AP heterogeneity - we call this approach rapid ionic current phenotyping (RICP). Features of this VC data were correlated to AP recordings from the same cells and we used computational models to generate mechanistic insights into cellular heterogeneity. This analysis uncovered several interesting links between AP morphology and ionic current density: both L-type calcium and sodium currents contribute to upstroke velocity, rapid delayed rectifier K+ current is the ma...
Source: American Journal of Physiology. Heart and Circulatory Physiology - March 15, 2024 Category: Physiology Authors: Alexander P Clark Siyu Wei Kristin Fullerton Trine Krogh-Madsen David J Christini Source Type: research

Surgically induced aortic coarctation in a neonatal porcine model allows for longitudinal assessment of cardiovascular changes
CONCLUSIONS: This model addresses many limitations of prior COA models including neonatal creation at an expected anatomic position with intimal injury and vessel sizes similar to humans.PMID:38488518 | DOI:10.1152/ajpheart.00087.2024 (Source: American Journal of Physiology. Heart and Circulatory Physiology)
Source: American Journal of Physiology. Heart and Circulatory Physiology - March 15, 2024 Category: Physiology Authors: Michael Stellon Leah Gober Matthew Allen Culver Joshua Hermsen Dana Irrer Colleen Witzenburg Alejandro Rold án-Alzate Luke Lamers Source Type: research

BAG3 localizes to mitochondria in cardiac fibroblasts and regulates mitophagy
Am J Physiol Heart Circ Physiol. 2024 Mar 15. doi: 10.1152/ajpheart.00736.2023. Online ahead of print.ABSTRACTThe co-chaperone BAG3 is a central node in protein quality control in the heart. In humans and animal models, decreased BAG3 expression is associated with cardiac dysfunction and dilated cardiomyopathy. While previous studies focused on BAG3 in cardiomyocytes, cardiac fibroblasts are also critical drivers of pathologic remodeling. Yet, BAG3's role in cardiac fibroblasts is almost completely unexplored. Here, we show BAG3 is expressed in primary rat neonatal cardiac fibroblasts and preferentially localizes to mitoch...
Source: American Journal of Physiology. Heart and Circulatory Physiology - March 15, 2024 Category: Physiology Authors: Thomas G Martin Laura A Sherer Jonathan A Kirk Source Type: research

Single-cell ionic current phenotyping explains stem cell-derived cardiomyocyte action potential morphology
In this study, we use recently published, brief (10 s), dynamic voltage clamp (VC) data to provide mechanistic insights into the ionic currents contributing to AP heterogeneity - we call this approach rapid ionic current phenotyping (RICP). Features of this VC data were correlated to AP recordings from the same cells and we used computational models to generate mechanistic insights into cellular heterogeneity. This analysis uncovered several interesting links between AP morphology and ionic current density: both L-type calcium and sodium currents contribute to upstroke velocity, rapid delayed rectifier K+ current is the ma...
Source: American Journal of Physiology. Heart and Circulatory Physiology - March 15, 2024 Category: Physiology Authors: Alexander P Clark Siyu Wei Kristin Fullerton Trine Krogh-Madsen David J Christini Source Type: research

Advanced maternal age alters cardiac functional and structural adaptations to pregnancy in rats
Am J Physiol Heart Circ Physiol. 2024 Mar 8. doi: 10.1152/ajpheart.00057.2024. Online ahead of print.ABSTRACTA significant number of pregnancies occur at advanced maternal age (>35 years), which is a risk factor for pregnancy complications. Healthy pregnancies require massive hemodynamic adaptations, including an increased blood volume and cardiac output. There is growing evidence that these cardiovascular adaptations are impaired with age, however, little is known about maternal cardiac function with advanced age. We hypothesized that cardiac adaptations to pregnancy are impaired with advanced maternal age. Younger (4 ...
Source: American Journal of Physiology. Heart and Circulatory Physiology - March 8, 2024 Category: Physiology Authors: Amy L Wooldridge Raven Kirschenman Floor Spaans Mazhar Pasha Sandra T Davidge Christy-Lynn M Cooke Source Type: research

Characterizing the influence of Cardiorespiratory Fitness on Left Atrial size and function in the general population
Am J Physiol Heart Circ Physiol. 2024 Mar 8. doi: 10.1152/ajpheart.00422.2023. Online ahead of print.ABSTRACTIncreased left atrial (LA) size and reduced LA function have been associated with heart failure and atrial fibrillation (AF) in at risk populations. However, atrial remodeling has also been associated with exercise training and the relationship between fitness, LA size and function has not been defined across the fitness spectrum. In a cross-sectional study of 559 ostensibly healthy participants, comprising 304 males (mean age 46 ± 20 years) and 255 females (mean age 47 ± 15 years), we sought to define the relatio...
Source: American Journal of Physiology. Heart and Circulatory Physiology - March 8, 2024 Category: Physiology Authors: Luke Spencer Leah Wright Stephen J Foulkes Stephanie J Rowe Hayley T Dillon Rachel Climie Ashley Bigaran Kristel Janssens Amy Mitchell Imogen Wallace Anniina Lindqvist Lauren Burnham David L Prior Erin J Howden Andr é La Gerche Source Type: research

Transient Low Shear Stress Preconditioning Influences Long-term Endothelial Traction and Alignment under High Shear Flow
Am J Physiol Heart Circ Physiol. 2024 Mar 8. doi: 10.1152/ajpheart.00067.2024. Online ahead of print.ABSTRACTEndothelial cells (ECs) within the vascular system encounter fluid shear stress (FSS). High, laminar FSS promotes vasodilation and anti-inflammatory responses, while low or disturbed FSS induces dysfunction and inflammation. However, the adaptation of ECs to dynamically changing FSS patterns remains underexplored. Here, by combining traction force microscopy with a custom flow chamber, we examined human umbilical vein endothelial cells adapting their traction during transitions from short-term low shear to long-term...
Source: American Journal of Physiology. Heart and Circulatory Physiology - March 8, 2024 Category: Physiology Authors: Mohanish K Chandurkar Nikhil Mittal Shaina P Royer-Weeden Steven D Lehmann Etienne B Michels Samuel E Haarman Scott A Severance Yeonwoo Rho Sangyoon J Han Source Type: research

Advanced maternal age alters cardiac functional and structural adaptations to pregnancy in rats
Am J Physiol Heart Circ Physiol. 2024 Mar 8. doi: 10.1152/ajpheart.00057.2024. Online ahead of print.ABSTRACTA significant number of pregnancies occur at advanced maternal age (>35 years), which is a risk factor for pregnancy complications. Healthy pregnancies require massive hemodynamic adaptations, including an increased blood volume and cardiac output. There is growing evidence that these cardiovascular adaptations are impaired with age, however, little is known about maternal cardiac function with advanced age. We hypothesized that cardiac adaptations to pregnancy are impaired with advanced maternal age. Younger (4 ...
Source: American Journal of Physiology. Heart and Circulatory Physiology - March 8, 2024 Category: Physiology Authors: Amy L Wooldridge Raven Kirschenman Floor Spaans Mazhar Pasha Sandra T Davidge Christy-Lynn M Cooke Source Type: research

Characterizing the influence of Cardiorespiratory Fitness on Left Atrial size and function in the general population
Am J Physiol Heart Circ Physiol. 2024 Mar 8. doi: 10.1152/ajpheart.00422.2023. Online ahead of print.ABSTRACTIncreased left atrial (LA) size and reduced LA function have been associated with heart failure and atrial fibrillation (AF) in at risk populations. However, atrial remodeling has also been associated with exercise training and the relationship between fitness, LA size and function has not been defined across the fitness spectrum. In a cross-sectional study of 559 ostensibly healthy participants, comprising 304 males (mean age 46 ± 20 years) and 255 females (mean age 47 ± 15 years), we sought to define the relatio...
Source: American Journal of Physiology. Heart and Circulatory Physiology - March 8, 2024 Category: Physiology Authors: Luke Spencer Leah Wright Stephen J Foulkes Stephanie J Rowe Hayley T Dillon Rachel Climie Ashley Bigaran Kristel Janssens Amy Mitchell Imogen Wallace Anniina Lindqvist Lauren Burnham David L Prior Erin J Howden Andr é La Gerche Source Type: research

Transient Low Shear Stress Preconditioning Influences Long-term Endothelial Traction and Alignment under High Shear Flow
Am J Physiol Heart Circ Physiol. 2024 Mar 8. doi: 10.1152/ajpheart.00067.2024. Online ahead of print.ABSTRACTEndothelial cells (ECs) within the vascular system encounter fluid shear stress (FSS). High, laminar FSS promotes vasodilation and anti-inflammatory responses, while low or disturbed FSS induces dysfunction and inflammation. However, the adaptation of ECs to dynamically changing FSS patterns remains underexplored. Here, by combining traction force microscopy with a custom flow chamber, we examined human umbilical vein endothelial cells adapting their traction during transitions from short-term low shear to long-term...
Source: American Journal of Physiology. Heart and Circulatory Physiology - March 8, 2024 Category: Physiology Authors: Mohanish K Chandurkar Nikhil Mittal Shaina P Royer-Weeden Steven D Lehmann Etienne B Michels Samuel E Haarman Scott A Severance Yeonwoo Rho Sangyoon J Han Source Type: research