Refactoring of a synthetic raspberry ketone pathway with EcoFlex
A key focus of synthetic biology is to develop microbial or cell-free based biobased routes to value-added chemicals such as fragrances. Originally, we developed the EcoFlex system, a Golden Gate toolkit, to ... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - June 10, 2021 Category: Microbiology Authors: Simon J. Moore, Yonek B. Hleba, Sarah Bischoff, David Bell, Karen M. Polizzi and Paul S. Freemont Tags: Research Source Type: research
An ADH toolbox for raspberry ketone production from natural resources via a biocatalytic cascade
Appl Microbiol Biotechnol. 2021 May 14. doi: 10.1007/s00253-021-11332-9. Online ahead of print.ABSTRACTRaspberry ketone is a widely used flavor compound in food and cosmetic industry. Several processes for its biocatalytic production have already been described, but either with the use of genetically modified organisms (GMOs) or incomplete conversion of the variety of precursors that are available in nature. Such natural precursors are rhododendrol glycosides with different proportions of (R)- and (S)-rhododendrol depending on the origin. After hydrolysis of these rhododendrol glycosides, the formed rhododendrol enantiomer...
Source: Applied Microbiology and Biotechnology - May 14, 2021 Category: Microbiology Authors: Aileen Becker Dominique B öttcher Werner Katzer Karsten Siems Lutz M üller-Kuhrt Uwe T Bornscheuer Source Type: research
Efficient bioconversion of raspberry ketone in Escherichia coli using fatty acids feedstocks
Phenylpropanoid including raspberry ketone, is a kind of important natural plant product and widely used in pharmaceuticals, chemicals, cosmetics, and healthcare products. Bioproduction of phenylpropanoid in Esch... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - March 12, 2021 Category: Microbiology Authors: Chen Chang, Bo Liu, Yihong Bao, Yong Tao and Weifeng Liu Tags: Research Source Type: research
[ASAP] Efficient Biosynthesis of Raspberry Ketone by Engineered < italic toggle="yes" > Escherichia coli < /italic > Coexpressing Zingerone Synthase and Glucose Dehydrogenase
Journal of Agricultural and Food ChemistryDOI: 10.1021/acs.jafc.0c07697 (Source: Journal of Agricultural and Food Chemistry)
Source: Journal of Agricultural and Food Chemistry - February 16, 2021 Category: Food Science Authors: Bo Yang, Pu Zheng, Dan Wu, and Pengcheng Chen Source Type: research
Molecules, Vol. 25, Pages 2877: Zingerone in the Flower of Passiflora Maliformis Attracts an Australian Fruit Fly, Bactrocera Jarvisi (Tryon)
lip W. Taylor
Passiflora maliformis is an introduced plant in Australia but its flowers are known to attract the native Jarvis&rsquo;s fruit fly, Bactrocera jarvisi (Tryon). The present study identifies and quantifies likely attractant(s) of male B. jarvisi in P. maliformis flowers. The chemical compositions of the inner and outer coronal filaments, anther, stigma, ovary, sepal, and petal of P. maliformis were separately extracted with ethanol and analyzed using gas chromatography-mass spectrometry (GC-MS). Polyisoprenoid lipid precursors, fatty acids and their derivatives, and phenylpropanoids were detected in...
Source: Molecules - June 21, 2020 Category: Chemistry Authors: Soo Jean Park Stefano G. De Faveri Jodie Cheesman Benjamin L. Hanssen Donald N. S. Cameron Ian M. Jamie Phillip W. Taylor Tags: Article Source Type: research
Acute feeding suppression and toxicity of raspberry ketone [4-(4-hydroxyphenyl)-2-butanone] in mice.
Abstract
Raspberry ketone (RK; [4-(4-hydroxyphenyl)-2-butanone]) is used by the food and cosmetic industry as a flavoring agent. RK is also marketed as a dietary supplement for weight maintenance and appetite control. The purpose of the study was to characterize the acute feeding suppression with RK (64-640 mg/kg) by oral gavage in male and female C57BL/6J mice. Cumulative 24 h food intake was reduced at 200 mg/kg (24% feeding suppression) in males and reliably reduced at 640 mg/kg (49-77% feeding suppression). Feeding suppression was not associated with pica behavior over the range of doses or conditioned...
Source: Food and Chemical Toxicology - June 17, 2020 Category: Food Science Authors: Hao L, Kshatriya D, Li X, Badrinath A, Szmacinski Z, Goedken MJ, Polunas M, Bello NT Tags: Food Chem Toxicol Source Type: research
Hepatoprotective effect of Raspberry ketone and white tea against acrylamide-induced toxicity in rats
. (Source: Drug and Chemical Toxicology)
Source: Drug and Chemical Toxicology - June 2, 2020 Category: Toxicology Authors: Soha M. Hamdy Zakaria El-Khayat Abdel Razik Farrag Ola N. Sayed Mervat M. El-Sayed Diaa Massoud Source Type: research
Synthesis of the character impact compound raspberry ketone and additional flavoring phenylbutanoids of biotechnological interest with Corynebacterium glutamicum
The phenylbutanoid 4-(4-hydroxyphenyl)butan-2-one, commonly known as raspberry ketone, is responsible for the typical scent and flavor of ripe raspberries. Chemical production of nature-identical raspberry ket... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - April 21, 2020 Category: Microbiology Authors: Lars Milke, Mario Mutz and Jan Marienhagen Tags: Research Source Type: research
Molecules, Vol. 25, Pages 1193: Chemometric Analysis of Low-field 1H NMR Spectra for Unveiling Adulteration of Slimming Dietary Supplements by Pharmaceutical Compounds
Gilard
The recent introduction of compact or low-field (LF) NMR spectrometers that use permanent magnets, giving rise to proton (1H) NMR frequencies between 40 and 80 MHz, have opened up new areas of application. The two main limitations of the technique are its insensitivity and poor spectral resolution. However, this study demonstrates that the chemometric treatment of LF 1H NMR spectral data is suitable for unveiling medicines as adulterants of slimming dietary supplements (DS). To this aim, 66 DS were analyzed with LF 1H NMR after quick and easy sample preparation. A first PLS-DA model built with the LF 1H NMR spec...
Source: Molecules - March 5, 2020 Category: Chemistry Authors: Nao Wu St éphane Balayssac Sa ïda Danoun Myriam Malet-Martino V éronique Gilard Tags: Communication Source Type: research
Influence of Diet ‐Induced Obesity on the Bioavailability and Metabolism of Raspberry Ketone (4‐(4‐Hydroxyphenyl)‐2‐Butanone) in Mice
ConclusionsRK was highly bioavailable, rapidly metabolized, and exhibited significantly different pharmacokinetic behaviors between obese and control mice. Lipid ‐rich tissues, especially WAT, could be a direct target of RK.This article is protected by copyright. All rights reserved (Source: Molecular Nutrition and Food Research)
Source: Molecular Nutrition and Food Research - February 13, 2020 Category: Food Science Authors: Danyue Zhao,
Bo Yuan,
Dushyant Kshatriya,
Andrew Polyak,
James E. Simon,
Nicholas T. Bello,
Qingli Wu Tags: Research Article Source Type: research
An Acetal Acylation Methodology for Producing Diversity of Trihalomethyl-1,3-dielectrophiles and 1,2-Azole Derivatives
A series of functionalized 1,1,1-trihalo-4-methoxy-3-alken-2-ones [CX3C(O)CR1=CROMe, where X = F or Cl; R = n-heptyl, n-octyl, n-nonyl, n-decyl, n-undecyl, n-tridecyl, (CH2)2CH=C(Me)2, (CH2)2Ph, (CH2)2-(4-HOC6H4), (CH2)2-(4-MeOC6H4), (CH2)2CO2Me, (CH2)3CO2Me, CH(SMe)CH3, CH2(2-MeOC6H4), and R1 = H, and R = H and R1 = n-decyl] were synthesized from respective alkyl methyl ketones or aldehyde via acetal acylation using trifluoroacetic anhydride and trichloroacetyl chloride. 1,1,1-Trihalo-4-methoxy-3-alken-2-ones with acid-compatible substituents were easily hydrolyzed to respective trihalomethyl-1,3-diketones. The 1,1,1-trih...
Source: Journal of the Brazilian Chemical Society - January 20, 2020 Category: Chemistry Source Type: research
Biochemical characterization of an organic solvent ‐tolerant glycosyltransferase from Bacillus licheniformis PI15 with potential application for raspberry ketone glycoside production
This article is protected by copyright. All rights reserved (Source: Biotechnology and Applied Biochemistry)
Source: Biotechnology and Applied Biochemistry - October 17, 2019 Category: Biochemistry Authors: Bingfeng Li,
Bo Fan,
Jingping Fan,
Siyuan Chang,
Xin Pan,
Yinan Wang,
Yaming Wu,
Junsong Song,
Xuejun He Tags: Original Article Source Type: research
Effect of Raspberry Ketone on Normal, Obese and Health-Compromised Obese Mice: A Preliminary Study.
This study suggests that higher doses of RK may cause adverse effects in health compromised conditions. Under these conditions, prolonged use of RK, especially in high doses, may pose a health hazard.
PMID: 31603036 [PubMed - as supplied by publisher] (Source: Journal of Dietary Supplements)
Source: Journal of Dietary Supplements - October 12, 2019 Category: Nutrition Tags: J Diet Suppl Source Type: research
