Anaerobic antibiotic coverage in aspiration pneumonia and the associated benefits and harms: A retrospective cohort study
Chest. 2024 Feb 20:S0012-3692(24)00260-5. doi: 10.1016/j.chest.2024.02.025. Online ahead of print.ABSTRACTBACKGROUND: Antibiotics with extended anaerobic coverage are commonly used to treat aspiration pneumonia, which is not recommended by current guidelines.RESEARCH QUESTION: In patients admitted to hospital for community-acquired aspiration pneumonia, is there a difference between antibiotic therapy with limited anaerobic coverage (LAC) versus antibiotic therapy with extended anaerobic coverage (EAC) in terms of in-hospital mortality and risk of Clostridioides difficile colitis?METHODS: We conducted a multicenter retrosp...
Source: Chest - February 22, 2024 Category: Respiratory Medicine Authors: Anthony D Bai Siddhartha Srivastava Genevi ève C Digby Vincent Girard Fahad Razak Amol A Verma Source Type: research
Comparative evaluation of antimicrobial efficacy of triple antibiotic paste and amoxicillin clavulanate paste as an intracanal medicament against Enterococcus faecalis: An in vitro study
Conclusion: Considering the limitations of this in vitro study, the findings suggest that ACP couldbe an effective alternative intracanal medicament to TAP for endodontic therapy.Key Words: Amoxicillin ‑potassium clavulanate combination, antibacterial agent, calcium hydroxide,ciprofloxacin, Enterococcus faecalis, metronidazole, minocycline (Source: Dental Research Journal)
Source: Dental Research Journal - February 21, 2024 Category: Dentistry Source Type: research
Electro-antibacterial therapy (EAT) to enhance intracellular bacteria clearance in pancreatic cancer cells
In this study, we investigate the use of electroporation in combination with metronidazole to enhance the clearance of intracellular Fusobacterium nucleatum within pancreatic cancer cells. We explore various parameters, including electric field strength, pulse width, and pulse number to assess the permeability of pancreatic cancer cells infected with F. nucleatum, compared to non-infected cells of the same type. We subsequently quantify the clearance of intracellular bacteria when these pulsing schemes are applied to a suspension of infected pancreatic cancer cells in the presence of metronidazole. Our results reveal disti...
Source: Bioelectrochemistry - February 20, 2024 Category: Biochemistry Authors: Josie L Duncan Raffae N Ahmad Hunter Danesi Daniel J Slade Rafael V Davalos Scott S Verbridge Source Type: research
Electro-antibacterial therapy (EAT) to enhance intracellular bacteria clearance in pancreatic cancer cells
In this study, we investigate the use of electroporation in combination with metronidazole to enhance the clearance of intracellular Fusobacterium nucleatum within pancreatic cancer cells. We explore various parameters, including electric field strength, pulse width, and pulse number to assess the permeability of pancreatic cancer cells infected with F. nucleatum, compared to non-infected cells of the same type. We subsequently quantify the clearance of intracellular bacteria when these pulsing schemes are applied to a suspension of infected pancreatic cancer cells in the presence of metronidazole. Our results reveal disti...
Source: Bioelectrochemistry - February 20, 2024 Category: Biochemistry Authors: Josie L Duncan Raffae N Ahmad Hunter Danesi Daniel J Slade Rafael V Davalos Scott S Verbridge Source Type: research
Deep eutectic solvent-based ferrofluid for highly efficient preconcentration and determination of metronidazole by vortex-assisted liquid-phase microextraction under experimental design optimization
In conclusion, the effectiveness of the vortex and ferrofluid combination, along with the convenience of collection and elimination of the need for centrifugation, bestows a highly valuable technique for determining metronidazole in water samples.PMID:38364554 | DOI:10.1016/j.talanta.2024.125705 (Source: Talanta)
Source: Talanta - February 16, 2024 Category: Chemistry Authors: Fatemeh Mehrabi Mehrorang Ghaedi Ebrahim Alipanahpour Dil Lobat Tayebi Source Type: research
Antibacterial activity of ibezapolstat against antimicrobial-resistant clinical strains of < em > Clostridioides difficile < /em >
This study demonstrated the potent antibacterial activity of IBZ against a large collection of C. difficile strains including multidrug-resistant strains. This study highlights the therapeutic potential of IBZ against multidrug-resistant strains of C. difficile.PMID:38364016 | DOI:10.1128/aac.01621-23 (Source: Antimicrobial Agents and Chemotherapy)
Source: Antimicrobial Agents and Chemotherapy - February 16, 2024 Category: Microbiology Authors: Eug énie Bassères Taryn A Eubank Khurshida Begum M Jahangir Alam Jinhee Jo Thanh M Le Chris K Lancaster Anne J Gonzales-Luna Kevin W Garey Source Type: research
Deep eutectic solvent-based ferrofluid for highly efficient preconcentration and determination of metronidazole by vortex-assisted liquid-phase microextraction under experimental design optimization
In conclusion, the effectiveness of the vortex and ferrofluid combination, along with the convenience of collection and elimination of the need for centrifugation, bestows a highly valuable technique for determining metronidazole in water samples.PMID:38364554 | DOI:10.1016/j.talanta.2024.125705 (Source: Talanta)
Source: Talanta - February 16, 2024 Category: Chemistry Authors: Fatemeh Mehrabi Mehrorang Ghaedi Ebrahim Alipanahpour Dil Lobat Tayebi Source Type: research
Antibacterial activity of ibezapolstat against antimicrobial-resistant clinical strains of < em > Clostridioides difficile < /em >
This study demonstrated the potent antibacterial activity of IBZ against a large collection of C. difficile strains including multidrug-resistant strains. This study highlights the therapeutic potential of IBZ against multidrug-resistant strains of C. difficile.PMID:38364016 | DOI:10.1128/aac.01621-23 (Source: Antimicrobial Agents and Chemotherapy)
Source: Antimicrobial Agents and Chemotherapy - February 16, 2024 Category: Microbiology Authors: Eug énie Bassères Taryn A Eubank Khurshida Begum M Jahangir Alam Jinhee Jo Thanh M Le Chris K Lancaster Anne J Gonzales-Luna Kevin W Garey Source Type: research
Antibacterial activity of ibezapolstat against antimicrobial-resistant clinical strains of < em > Clostridioides difficile < /em >
This study demonstrated the potent antibacterial activity of IBZ against a large collection of C. difficile strains including multidrug-resistant strains. This study highlights the therapeutic potential of IBZ against multidrug-resistant strains of C. difficile.PMID:38364016 | DOI:10.1128/aac.01621-23 (Source: Antimicrobial Agents and Chemotherapy)
Source: Antimicrobial Agents and Chemotherapy - February 16, 2024 Category: Microbiology Authors: Eug énie Bassères Taryn A Eubank Khurshida Begum M Jahangir Alam Jinhee Jo Thanh M Le Chris K Lancaster Anne J Gonzales-Luna Kevin W Garey Source Type: research
Prevalence and antimicrobial resistance of bacterial enteropathogens in Crete, Greece, during 2011-2022
This study investigated the epidemiology and the antibiotic susceptibility of bacterial enteropathogens among diarrheal patients of all ages in Crete, Greece during 2011-2022. Stool specimens were tested by conventional cultural methods for Salmonella, Shigella, Campylobacter, diarrheagenic Escherichia coli (EPEC, STEC), Yersinia enterocolitica, Aeromonas species and Clostridioides difficile. Antimicrobial susceptibility was determined by the disk diffusion method for Enterobacterales, Campylobacter and Aeromonas, and by the gradient diffusion method for C. difficile. Of the 26,060 stool samples from patients of any age, 1...
Source: Acta Microbiologica et Immunologica Hungarica - February 12, 2024 Category: Microbiology Authors: Sofia Maraki Viktoria Eirini Mavromanolaki Dimitra Stafylaki Evangelia Iliaki-Giannakoudaki Anna Kasimati Source Type: research
The efficacy of combining adjuvants with non-surgical periodontal therapy in individuals with type 2 diabetes: A Bayesian network meta-analysis
CONCLUSIONS: Adjuvant use of metronidazole or alpha lipoic acid was the best adjunct option to provide clinically meaningful HbA1c levels and probing depth reductions. However, no strong recommendation can be drawn due to the scarcity of studies for each adjuvant treatment and the low certainty of the resultant evidence.PMID:38342946 | DOI:10.1111/jcpe.13946 (Source: Journal of Clinical Periodontology)
Source: Journal of Clinical Periodontology - February 11, 2024 Category: Dentistry Authors: Fabr ício Batistin Zanatta Raquel Pippi Antoniazzi Leandro Machado Oliveira Adam D Lietzan Patricia A Miguez Cristiano Susin Source Type: research
Nutritional Value and in vitro Antigiardial Activity of Anise (Pimpinella anisum L.) Seeds
Chem Biodivers. 2024 Feb 9:e202301254. doi: 10.1002/cbdv.202301254. Online ahead of print.ABSTRACTThe present study focused on evaluating the proximate analysis, mineral composition, and in vitro anti-giardial activity of Pimpinella anisum seed extracts, which are aromatic plants with a long history of usage in folk and conventional medicine, as well as pharmaceutical manufacturing. Standard methods were used to determine the proximate analysis of the powdered plant sample, including dry matter, ash, fat, protein, fiber, and carbohydrates. The mineral contents of Pimpinella anisum seed were analyzed using Inductively Coupl...
Source: Chemistry and Biodiversity - February 9, 2024 Category: Biochemistry Authors: Ayat Ahmed Alrasheid Ahmed Saeed Kabbashi Amar Osman Ali Amel Ahmed Alrasheed Zeinab Kanani Missa Mohammed Saleh Gokhan Zengin Saad Mohammed Hussein Ayoub Source Type: research
GSE253746 Interactions of erythromycin and an antibiotic-mixture to the gut microbiome of juvenile rainbow trout
The objectives of experiment 1 were to understand uptake and depuration of ERY in juvenile rainbow trout (RBT) over a 7 d uptake followed by a 7 d depuration period using three concentrations of ERY. Furthermore, throughout the study changes in gut microbiome response were assessed. In experiment 2, a follow-up study was conducted using an identical experimental design to assess the impacts of an antibiotic-mixture (ERY, ampicillin, metronidazole, and ciprofloxacin at 100 µg/g each). Here, three matrices were analyzed, with gut collected for 16s metabarcoding, plasma for untargeted metabolomics, and brain for mRNA-seq ana...
Source: GEO: Gene Expression Omnibus - February 7, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Source Type: research
Inhibition of choline kinase as an antiamoebic approach in Entamoeba histolytica infection
This study investigates a novel anti-amoebic approach targeting the CDP-choline pathway. Inhibition studies were conducted using potential choline kinase (CK) inhibitors to inhibit the EhCK enzyme, and RNA interference was employed to knock down the EhCK gene. Km and Vmax of purified EhCK and hCKa2 proteins were determined by pyruvate kinase-lactate dehydrogenase (PK-LDH) coupled assay. The IC50 values for EhCK and hCKa2 were determined with several commercial CK inhibitors. Selected inhibitors were incubated with E. histolytica trophozoites for 48 hours to determine the EC50 for each inhibitor. Silencing of gene encoding ...
Source: Tropical Biomedicine - February 3, 2024 Category: Tropical Medicine Authors: Z H Teh B H Lim W C See Too L L Few Source Type: research
Inhibition of choline kinase as an antiamoebic approach in Entamoeba histolytica infection
This study investigates a novel anti-amoebic approach targeting the CDP-choline pathway. Inhibition studies were conducted using potential choline kinase (CK) inhibitors to inhibit the EhCK enzyme, and RNA interference was employed to knock down the EhCK gene. Km and Vmax of purified EhCK and hCKa2 proteins were determined by pyruvate kinase-lactate dehydrogenase (PK-LDH) coupled assay. The IC50 values for EhCK and hCKa2 were determined with several commercial CK inhibitors. Selected inhibitors were incubated with E. histolytica trophozoites for 48 hours to determine the EC50 for each inhibitor. Silencing of gene encoding ...
Source: Tropical Biomedicine - February 3, 2024 Category: Tropical Medicine Authors: Z H Teh B H Lim W C See Too L L Few Source Type: research
