Evaluation of Various < em > Escherichia coli < /em > Strains for Enhanced Lycopene Production
In this study, we evaluated 16 E. coli strains to determine the best lycopene production host by introducing a lycopene biosynthetic pathway (crtE, crtB, and crtI genes cloned from Deinococcus wulumuqiensis R12 and dxs, dxr, ispA, and idi genes cloned from E. coli). The 16 lycopene strain titers diverged from 0 to 0.141 g/l, with MG1655 demonstrating the highest titer (0.141 g/l), while the SURE and W strains expressed the lowest (0 g/l) in an LB medium. When a 2 × YTg medium replaced the MG1655 culture medium, the titer further escalated to 1.595 g/l. These results substantiate that strain selection is vital in metabolic...
Source: Journal of Microbiology and Biotechnology - April 26, 2023 Category: Biotechnology Authors: Jun Ren Junhao Shen Thi Duc Thai Min-Gyun Kim Seung Ho Lee Wonseop Lim Dokyun Na Source Type: research
Evaluation of Various < em > Escherichia coli < /em > Strains for Enhanced Lycopene Production
In this study, we evaluated 16 E. coli strains to determine the best lycopene production host by introducing a lycopene biosynthetic pathway (crtE, crtB, and crtI genes cloned from Deinococcus wulumuqiensis R12 and dxs, dxr, ispA, and idi genes cloned from E. coli). The 16 lycopene strain titers diverged from 0 to 0.141 g/l, with MG1655 demonstrating the highest titer (0.141 g/l), while the SURE and W strains expressed the lowest (0 g/l) in an LB medium. When a 2 × YTg medium replaced the MG1655 culture medium, the titer further escalated to 1.595 g/l. These results substantiate that strain selection is vital in metabolic...
Source: Journal of Microbiology and Biotechnology - April 26, 2023 Category: Biotechnology Authors: Jun Ren Junhao Shen Thi Duc Thai Min-Gyun Kim Seung Ho Lee Wonseop Lim Dokyun Na Source Type: research
Evaluation of Various < em > Escherichia coli < /em > Strains for Enhanced Lycopene Production
In this study, we evaluated 16 E. coli strains to determine the best lycopene production host by introducing a lycopene biosynthetic pathway (crtE, crtB, and crtI genes cloned from Deinococcus wulumuqiensis R12 and dxs, dxr, ispA, and idi genes cloned from E. coli). The 16 lycopene strain titers diverged from 0 to 0.141 g/l, with MG1655 demonstrating the highest titer (0.141 g/l), while the SURE and W strains expressed the lowest (0 g/l) in an LB medium. When a 2 × YTg medium replaced the MG1655 culture medium, the titer further escalated to 1.595 g/l. These results substantiate that strain selection is vital in metabolic...
Source: Journal of Microbiology and Biotechnology - April 26, 2023 Category: Biotechnology Authors: Jun Ren Junhao Shen Thi Duc Thai Min-Gyun Kim Seung Ho Lee Wonseop Lim Dokyun Na Source Type: research
Evaluation of Various < em > Escherichia coli < /em > Strains for Enhanced Lycopene Production
In this study, we evaluated 16 E. coli strains to determine the best lycopene production host by introducing a lycopene biosynthetic pathway (crtE, crtB, and crtI genes cloned from Deinococcus wulumuqiensis R12 and dxs, dxr, ispA, and idi genes cloned from E. coli). The 16 lycopene strain titers diverged from 0 to 0.141 g/l, with MG1655 demonstrating the highest titer (0.141 g/l), while the SURE and W strains expressed the lowest (0 g/l) in an LB medium. When a 2 × YTg medium replaced the MG1655 culture medium, the titer further escalated to 1.595 g/l. These results substantiate that strain selection is vital in metabolic...
Source: Journal of Microbiology and Biotechnology - April 26, 2023 Category: Biotechnology Authors: Jun Ren Junhao Shen Thi Duc Thai Min-Gyun Kim Seung Ho Lee Wonseop Lim Dokyun Na Source Type: research
Evaluation of Various < em > Escherichia coli < /em > Strains for Enhanced Lycopene Production
In this study, we evaluated 16 E. coli strains to determine the best lycopene production host by introducing a lycopene biosynthetic pathway (crtE, crtB, and crtI genes cloned from Deinococcus wulumuqiensis R12 and dxs, dxr, ispA, and idi genes cloned from E. coli). The 16 lycopene strain titers diverged from 0 to 0.141 g/l, with MG1655 demonstrating the highest titer (0.141 g/l), while the SURE and W strains expressed the lowest (0 g/l) in an LB medium. When a 2 × YTg medium replaced the MG1655 culture medium, the titer further escalated to 1.595 g/l. These results substantiate that strain selection is vital in metabolic...
Source: Journal of Microbiology and Biotechnology - April 26, 2023 Category: Biotechnology Authors: Jun Ren Junhao Shen Thi Duc Thai Min-Gyun Kim Seung Ho Lee Wonseop Lim Dokyun Na Source Type: research
Evaluation of Various < em > Escherichia coli < /em > Strains for Enhanced Lycopene Production
In this study, we evaluated 16 E. coli strains to determine the best lycopene production host by introducing a lycopene biosynthetic pathway (crtE, crtB, and crtI genes cloned from Deinococcus wulumuqiensis R12 and dxs, dxr, ispA, and idi genes cloned from E. coli). The 16 lycopene strain titers diverged from 0 to 0.141 g/l, with MG1655 demonstrating the highest titer (0.141 g/l), while the SURE and W strains expressed the lowest (0 g/l) in an LB medium. When a 2 × YTg medium replaced the MG1655 culture medium, the titer further escalated to 1.595 g/l. These results substantiate that strain selection is vital in metabolic...
Source: Journal of Microbiology and Biotechnology - April 26, 2023 Category: Biotechnology Authors: Jun Ren Junhao Shen Thi Duc Thai Min-Gyun Kim Seung Ho Lee Wonseop Lim Dokyun Na Source Type: research
Evaluation of Various < em > Escherichia coli < /em > Strains for Enhanced Lycopene Production
In this study, we evaluated 16 E. coli strains to determine the best lycopene production host by introducing a lycopene biosynthetic pathway (crtE, crtB, and crtI genes cloned from Deinococcus wulumuqiensis R12 and dxs, dxr, ispA, and idi genes cloned from E. coli). The 16 lycopene strain titers diverged from 0 to 0.141 g/l, with MG1655 demonstrating the highest titer (0.141 g/l), while the SURE and W strains expressed the lowest (0 g/l) in an LB medium. When a 2 × YTg medium replaced the MG1655 culture medium, the titer further escalated to 1.595 g/l. These results substantiate that strain selection is vital in metabolic...
Source: Journal of Microbiology and Biotechnology - April 26, 2023 Category: Biotechnology Authors: Jun Ren Junhao Shen Thi Duc Thai Min-Gyun Kim Seung Ho Lee Wonseop Lim Dokyun Na Source Type: research
Evaluation of Various < em > Escherichia coli < /em > Strains for Enhanced Lycopene Production
In this study, we evaluated 16 E. coli strains to determine the best lycopene production host by introducing a lycopene biosynthetic pathway (crtE, crtB, and crtI genes cloned from Deinococcus wulumuqiensis R12 and dxs, dxr, ispA, and idi genes cloned from E. coli). The 16 lycopene strain titers diverged from 0 to 0.141 g/l, with MG1655 demonstrating the highest titer (0.141 g/l), while the SURE and W strains expressed the lowest (0 g/l) in an LB medium. When a 2 × YTg medium replaced the MG1655 culture medium, the titer further escalated to 1.595 g/l. These results substantiate that strain selection is vital in metabolic...
Source: Journal of Microbiology and Biotechnology - April 26, 2023 Category: Biotechnology Authors: Jun Ren Junhao Shen Thi Duc Thai Min-Gyun Kim Seung Ho Lee Wonseop Lim Dokyun Na Source Type: research
Evaluation of Various < em > Escherichia coli < /em > Strains for Enhanced Lycopene Production
In this study, we evaluated 16 E. coli strains to determine the best lycopene production host by introducing a lycopene biosynthetic pathway (crtE, crtB, and crtI genes cloned from Deinococcus wulumuqiensis R12 and dxs, dxr, ispA, and idi genes cloned from E. coli). The 16 lycopene strain titers diverged from 0 to 0.141 g/l, with MG1655 demonstrating the highest titer (0.141 g/l), while the SURE and W strains expressed the lowest (0 g/l) in an LB medium. When a 2 × YTg medium replaced the MG1655 culture medium, the titer further escalated to 1.595 g/l. These results substantiate that strain selection is vital in metabolic...
Source: Journal of Microbiology and Biotechnology - April 26, 2023 Category: Biotechnology Authors: Jun Ren Junhao Shen Thi Duc Thai Min-Gyun Kim Seung Ho Lee Wonseop Lim Dokyun Na Source Type: research
Evaluation of Various < em > Escherichia coli < /em > Strains for Enhanced Lycopene Production
In this study, we evaluated 16 E. coli strains to determine the best lycopene production host by introducing a lycopene biosynthetic pathway (crtE, crtB, and crtI genes cloned from Deinococcus wulumuqiensis R12 and dxs, dxr, ispA, and idi genes cloned from E. coli). The 16 lycopene strain titers diverged from 0 to 0.141 g/l, with MG1655 demonstrating the highest titer (0.141 g/l), while the SURE and W strains expressed the lowest (0 g/l) in an LB medium. When a 2 × YTg medium replaced the MG1655 culture medium, the titer further escalated to 1.595 g/l. These results substantiate that strain selection is vital in metabolic...
Source: Journal of Microbiology and Biotechnology - April 26, 2023 Category: Biotechnology Authors: Jun Ren Junhao Shen Thi Duc Thai Min-Gyun Kim Seung Ho Lee Wonseop Lim Dokyun Na Source Type: research
Evaluation of Various < em > Escherichia coli < /em > Strains for Enhanced Lycopene Production
In this study, we evaluated 16 E. coli strains to determine the best lycopene production host by introducing a lycopene biosynthetic pathway (crtE, crtB, and crtI genes cloned from Deinococcus wulumuqiensis R12 and dxs, dxr, ispA, and idi genes cloned from E. coli). The 16 lycopene strain titers diverged from 0 to 0.141 g/l, with MG1655 demonstrating the highest titer (0.141 g/l), while the SURE and W strains expressed the lowest (0 g/l) in an LB medium. When a 2 × YTg medium replaced the MG1655 culture medium, the titer further escalated to 1.595 g/l. These results substantiate that strain selection is vital in metabolic...
Source: Journal of Microbiology and Biotechnology - April 26, 2023 Category: Biotechnology Authors: Jun Ren Junhao Shen Thi Duc Thai Min-Gyun Kim Seung Ho Lee Wonseop Lim Dokyun Na Source Type: research
Lycopene inhibits endothelial-to-mesenchymal transition of choroidal vascular endothelial cells in laser-induced mouse choroidal neovascularization
J Cell Mol Med. 2023 Apr 17. doi: 10.1111/jcmm.17730. Online ahead of print.ABSTRACTChoroidal neovascularization (CNV), is a major cause of irreversible blindness among the elderly population in developed countries, which is resulted from subretinal fibrosis without effective therapeutic strategies. Endothelial-to-mesenchymal transition (EndMT) of choroidal vascular endothelial cells (CVECs) contributes to subretinal fibrosis. Lycopene (LYC), a non-pro-vitamin A carotenoid, plays an anti-fibrotic role. Herein, we explored the effect and mechanism of LYC on the EndMT of CVECs during CNV. Firstly, LYC inhibited EndMT in hypo...
Source: J Cell Mol Med - April 18, 2023 Category: Molecular Biology Authors: Lele Li Xin Cao Lili Huang Xiaobo Huang Jiayi Gu Xiaoli Yu Yan Zhu Yue Zhou Yu Song Manhui Zhu Source Type: research
Lycopene inhibits endothelial-to-mesenchymal transition of choroidal vascular endothelial cells in laser-induced mouse choroidal neovascularization
J Cell Mol Med. 2023 Apr 17. doi: 10.1111/jcmm.17730. Online ahead of print.ABSTRACTChoroidal neovascularization (CNV), is a major cause of irreversible blindness among the elderly population in developed countries, which is resulted from subretinal fibrosis without effective therapeutic strategies. Endothelial-to-mesenchymal transition (EndMT) of choroidal vascular endothelial cells (CVECs) contributes to subretinal fibrosis. Lycopene (LYC), a non-pro-vitamin A carotenoid, plays an anti-fibrotic role. Herein, we explored the effect and mechanism of LYC on the EndMT of CVECs during CNV. Firstly, LYC inhibited EndMT in hypo...
Source: Molecular Medicine - April 18, 2023 Category: Molecular Biology Authors: Lele Li Xin Cao Lili Huang Xiaobo Huang Jiayi Gu Xiaoli Yu Yan Zhu Yue Zhou Yu Song Manhui Zhu Source Type: research
Lycopene inhibits endothelial-to-mesenchymal transition of choroidal vascular endothelial cells in laser-induced mouse choroidal neovascularization
J Cell Mol Med. 2023 Apr 17. doi: 10.1111/jcmm.17730. Online ahead of print.ABSTRACTChoroidal neovascularization (CNV), is a major cause of irreversible blindness among the elderly population in developed countries, which is resulted from subretinal fibrosis without effective therapeutic strategies. Endothelial-to-mesenchymal transition (EndMT) of choroidal vascular endothelial cells (CVECs) contributes to subretinal fibrosis. Lycopene (LYC), a non-pro-vitamin A carotenoid, plays an anti-fibrotic role. Herein, we explored the effect and mechanism of LYC on the EndMT of CVECs during CNV. Firstly, LYC inhibited EndMT in hypo...
Source: J Cell Mol Med - April 18, 2023 Category: Molecular Biology Authors: Lele Li Xin Cao Lili Huang Xiaobo Huang Jiayi Gu Xiaoli Yu Yan Zhu Yue Zhou Yu Song Manhui Zhu Source Type: research
Lycopene inhibits endothelial-to-mesenchymal transition of choroidal vascular endothelial cells in laser-induced mouse choroidal neovascularization
J Cell Mol Med. 2023 Apr 17. doi: 10.1111/jcmm.17730. Online ahead of print.ABSTRACTChoroidal neovascularization (CNV), is a major cause of irreversible blindness among the elderly population in developed countries, which is resulted from subretinal fibrosis without effective therapeutic strategies. Endothelial-to-mesenchymal transition (EndMT) of choroidal vascular endothelial cells (CVECs) contributes to subretinal fibrosis. Lycopene (LYC), a non-pro-vitamin A carotenoid, plays an anti-fibrotic role. Herein, we explored the effect and mechanism of LYC on the EndMT of CVECs during CNV. Firstly, LYC inhibited EndMT in hypo...
Source: Molecular Medicine - April 18, 2023 Category: Molecular Biology Authors: Lele Li Xin Cao Lili Huang Xiaobo Huang Jiayi Gu Xiaoli Yu Yan Zhu Yue Zhou Yu Song Manhui Zhu Source Type: research
