Site-directed mutagenesis at the Glu78 in Ec-NhaA transporter impacting ion exchange: a biophysical study
In this study, we have explored the role of Glu78 in the regulation of pH by Ec-NhaA. Although we have created various mutants, E78C has shown a considerable effect on the stoichiometry of NhaA and presented comparable phenotypes. The ITC experiment has shown the binding of ~ 5 protons in response to the transport of one lithium ion. The phenotype analysis on selective medium showed a significant expression compared to WT Ec-NhaA. This represents the importance of Glu78 in transporting the H+ across the membrane where a single mutation with Cys amino acid alters the number of H+ significantly maintaining the activity of th...
Source: European Biophysics Journal : EBJ - April 22, 2024 Category: Physics Authors: Anuradha Yadav Dinesh Kumar Manish Dwivedi Source Type: research
Site-directed mutagenesis at the Glu78 in Ec-NhaA transporter impacting ion exchange: a biophysical study
In this study, we have explored the role of Glu78 in the regulation of pH by Ec-NhaA. Although we have created various mutants, E78C has shown a considerable effect on the stoichiometry of NhaA and presented comparable phenotypes. The ITC experiment has shown the binding of ~ 5 protons in response to the transport of one lithium ion. The phenotype analysis on selective medium showed a significant expression compared to WT Ec-NhaA. This represents the importance of Glu78 in transporting the H+ across the membrane where a single mutation with Cys amino acid alters the number of H+ significantly maintaining the activity of th...
Source: European Biophysics Journal : EBJ - April 22, 2024 Category: Physics Authors: Anuradha Yadav Dinesh Kumar Manish Dwivedi Source Type: research
Site-directed mutagenesis at the Glu78 in Ec-NhaA transporter impacting ion exchange: a biophysical study
In this study, we have explored the role of Glu78 in the regulation of pH by Ec-NhaA. Although we have created various mutants, E78C has shown a considerable effect on the stoichiometry of NhaA and presented comparable phenotypes. The ITC experiment has shown the binding of ~ 5 protons in response to the transport of one lithium ion. The phenotype analysis on selective medium showed a significant expression compared to WT Ec-NhaA. This represents the importance of Glu78 in transporting the H+ across the membrane where a single mutation with Cys amino acid alters the number of H+ significantly maintaining the activity of th...
Source: European Biophysics Journal : EBJ - April 22, 2024 Category: Physics Authors: Anuradha Yadav Dinesh Kumar Manish Dwivedi Source Type: research
Site-directed mutagenesis at the Glu78 in Ec-NhaA transporter impacting ion exchange: a biophysical study
In this study, we have explored the role of Glu78 in the regulation of pH by Ec-NhaA. Although we have created various mutants, E78C has shown a considerable effect on the stoichiometry of NhaA and presented comparable phenotypes. The ITC experiment has shown the binding of ~ 5 protons in response to the transport of one lithium ion. The phenotype analysis on selective medium showed a significant expression compared to WT Ec-NhaA. This represents the importance of Glu78 in transporting the H+ across the membrane where a single mutation with Cys amino acid alters the number of H+ significantly maintaining the activity of th...
Source: European Biophysics Journal : EBJ - April 22, 2024 Category: Physics Authors: Anuradha Yadav Dinesh Kumar Manish Dwivedi Source Type: research
Site-directed mutagenesis at the Glu78 in Ec-NhaA transporter impacting ion exchange: a biophysical study
In this study, we have explored the role of Glu78 in the regulation of pH by Ec-NhaA. Although we have created various mutants, E78C has shown a considerable effect on the stoichiometry of NhaA and presented comparable phenotypes. The ITC experiment has shown the binding of ~ 5 protons in response to the transport of one lithium ion. The phenotype analysis on selective medium showed a significant expression compared to WT Ec-NhaA. This represents the importance of Glu78 in transporting the H+ across the membrane where a single mutation with Cys amino acid alters the number of H+ significantly maintaining the activity of th...
Source: European Biophysics Journal : EBJ - April 22, 2024 Category: Physics Authors: Anuradha Yadav Dinesh Kumar Manish Dwivedi Source Type: research
Site-directed mutagenesis at the Glu78 in Ec-NhaA transporter impacting ion exchange: a biophysical study
In this study, we have explored the role of Glu78 in the regulation of pH by Ec-NhaA. Although we have created various mutants, E78C has shown a considerable effect on the stoichiometry of NhaA and presented comparable phenotypes. The ITC experiment has shown the binding of ~ 5 protons in response to the transport of one lithium ion. The phenotype analysis on selective medium showed a significant expression compared to WT Ec-NhaA. This represents the importance of Glu78 in transporting the H+ across the membrane where a single mutation with Cys amino acid alters the number of H+ significantly maintaining the activity of th...
Source: European Biophysics Journal : EBJ - April 22, 2024 Category: Physics Authors: Anuradha Yadav Dinesh Kumar Manish Dwivedi Source Type: research
LiF/Li3N ‐Rich Electrode–Electrolyte Interfaces Enabled by Multi‐Functional Electrolyte Additive to Achieve High‐Performance Li/LiNi0.8Co0.1Mn0.1O2 Batteries
By adding N-succinimidyl trifluoroacetate (NST) as an innovative and multifunctional additive into conventional LiPF6-based carbonate electrolyte, the ability to simultaneously in situ construct bi-electrodes-electrolyte LiF/Li3N-rich interphases with high Li+ conductivity and optimize the electrolyte system via removing HF/H2O and affecting Li+ solvation structure are well achieved. Thus, the electrochemical performances and safety of high-energy-density rechargeable Li/LiNi0.8Co0.1Mn0.1O2 batteries are remarkably boosted. AbstractLiPF6-based carbonate electrolytes have been extensively employed in commercial Li-ion batte...
Source: Small - April 22, 2024 Category: Nanotechnology Authors: Yue Lei,
Xin Xu,
Junying Yin,
Kang Xi,
Lai Wei,
Junzi Zheng,
Yuhao Wang,
Haihua Wu,
Sen Jiang,
Yunfang Gao Tags: Research Article Source Type: research
Understanding the Coupling Mechanism of Intercalation and Conversion Hybrid Storage in Lithium –Graphite Anode
The working mechanisms of lithium –graphite composite anode are comprehensively investigated by analyzing the capacity decay ratios under different specific capacities. The capacity decay models of composite anode including four stages can be reached with intercalation and conversion hybrid storage mechanism. These results can pre sent novel insights for working mechanisms and design principles of high-capacity anode materials. AbstractAnodes with high capacity and long lifespan play an important role in the advanced batteries. However, none of the existing anodes can meet these two requirements simultaneously. Lithium (...
Source: Small - April 22, 2024 Category: Nanotechnology Authors: Xin Sun,
He Liu,
Ke ‐Feng Ren,
Wen‐Bo Tang,
Cong Guo,
Weizhai Bao,
Feng Yu,
Xin‐Bing Cheng,
Jingfa Li Tags: Research Article Source Type: research
How to freeze a memory: putting worms on ice stops them forgetting
Nature, Published online: 22 April 2024; doi:10.1038/d41586-024-01130-4The model organism Caenorhabditis elegans is quick to forget a notable odour — unless it is chilled or given lithium. (Source: Nature AOP)
Source: Nature AOP - April 22, 2024 Category: Research Authors: Julian Nowogrodzki Source Type: research
Determination of a whole-blood single-test low-risk threshold for a point-of-care high-sensitivity troponin assay
High-sensitivity troponin (hsTn) is used with ECG and clinical evaluation to stratify patients attending ED as low risk for acute myocardial infarctions (AMI). Diagnostic pathways incorporate a single test threshold for stratification with these assays. The Siemens Atellica VTLi Point-Of-Care-hsTnI assay (POC-hsTnI) has an 8 min turn-around which may expedite decision-making. Although a single-test low-risk (‘rule-out’) threshold for this assay has been reported,1 it was desirable to determine a threshold specifically for New Zealand ED settings and intended end-users. From 3 November 2022 to 28 May 2023, hsTnI...
Source: Emergency Medicine Journal - April 22, 2024 Category: Emergency Medicine Authors: Pickering, J. W., Hamill, L., Aldous, S., Joyce, L., Stothart, R. A., Williams, O., Florkowski, C. M., Than, M. Tags: Research letter Source Type: research
Lithium Pre-Storage Enables High Initial Coulombic Efficiency and Stable Lithium-Enriched Silicon/Graphite Anode
This study opens up a promising avenue for highly air and water-stable silicon anode prelithiation methods.PMID:38644436 | DOI:10.1002/anie.202404637 (Source: Angewandte Chemie)
Source: Angewandte Chemie - April 21, 2024 Category: Chemistry Authors: Yingjie Gao Chenghao Cui Zhikun Huang Guoyu Pan Yuanfan Gu Yanan Yang Fan Bai Zhuang Sun Tao Zhang Source Type: research
A Comprehensive Review on Reductive Recycling of Cathode Materials of Spent Lithium-Ion Batteries
Chemistry. 2024 Apr 20:e202400566. doi: 10.1002/chem.202400566. Online ahead of print.ABSTRACTThe prosperity of the lithium-ion battery market is inevitably accompanied by the depletion of corresponding resources and the accumulation of spent batteries in a dialectical manner. Spent lithium-ion batteries are harboring the characteristics of hazardous waste and high-value resources, so efficient recycling is of great significance. The cathode material is considered as an interesting target for repurposing. Despite some important reviews give commendable emphasis to recycling technologies, there is still a dearth of explorat...
Source: Chemistry - April 20, 2024 Category: Chemistry Authors: Yiran Li Junhui Cai Jiayu Wang Shengnv Xu Yanjuan Li Wei He Zhanzhan Wang Shun Yang Xiao Yan Source Type: research
Lithium induced neurotoxicity presenting as a rapidly progressive encephalopathy: a case report and initial evidence for an immune-mediated pathophysiology
(Source: Acta Neurologica Belgica)
Source: Acta Neurologica Belgica - April 20, 2024 Category: Neurology Source Type: research
Stability and Structure of the Aqueous LiTFSI-LiCl Interface
This article is licensed under aCreative Commons Attribution 3.0 Unported Licence.Hannah Wood, Hannah M Burnett, Robert Dryfe, Paola Carbone It has recently been demonstrated that aqueous lithium bis(trifluoromethanesulfonyl)imide (LiTFSI) and lithium chloride (LiCl) solutions can form stable liquid-liquid biphasic systems when both elec- trolyte phases have sufficiently high concentrations.... The content of this RSS Feed (c) The Royal Society of Chemistry (Source: RSC - Faraday Discuss. latest articles)
Source: RSC - Faraday Discuss. latest articles - April 19, 2024 Category: Chemistry Authors: Hannah Wood Source Type: research
