Isolation of local strains of the yeast Metschnikowia for biocontrol and lipid production purposes
AbstractThe bioprospection of indigenous microorganism strains with biotechnological potential represents a prominent trend.Metschnikowia yeasts exhibit diverse capabilities, such as ethanol reduction in winemaking, biocontrol potential, and lipid production. In this work, localMetschnikowia strains were isolated from different fruits by their ability to produce pulcherrimic acid, a molecule that has been linked to biocontrol activity and that binds iron giving colored colonies. Five strains were selected, each from one of five distinct sources. All of them were identified asM. pulcherrima. All five were able inhibit other...
Source: World Journal of Microbiology and Biotechnology - February 9, 2024 Category: Microbiology Source Type: research

Two at once: simultaneous increased production of astaxanthin and mycosporines in a single batch culture using a Phaffia rhodozyma mutant strain
AbstractPhaffia rhodozyma is a basidiomycetous yeast characterized by its production of the carotenoid pigment astaxanthin, which holds high commercial value for its significance in aquaculture, cosmetics and as nutraceutics, and the UV-B-absorbing compound mycosporine-glutaminol-glucoside (MGG), which is of great biotechnological relevance for its incorporation into natural sunscreens. However, the industrial exploitation has been limited to the production of astaxanthin in small quantities. On the other hand, the accumulation of MGG inP. rhodozyma was recently reported and could add value to the simultaneous production o...
Source: World Journal of Microbiology and Biotechnology - February 8, 2024 Category: Microbiology Source Type: research

Antimicrobial and antibiofilm properties of selenium-chitosan-loaded salicylic acid nanoparticles for the removal of emerging contaminants from bacterial pathogens
In this study salicylic acid loaded containing selenium nanoparticles was synthesized and called SA@CS-Se NPs. the chitosan was used as a natural stabilizer during the synthesis process. Fourier transforms infrared spectroscopy (FTIR), Powder X-ray diffraction (XRD), field emission electron microscopy (FESEM), and transmission electron microscopy (TEM) were used to describe the physicochemical characteristics of the SA@CS-Se NPs. The PXRD examination revealed that the grain size was around 31.9  nm. TEM and FESEM techniques showed the spherical shape of SA@CS-Se NPs. Additionally, the analysis of experiments showed that S...
Source: World Journal of Microbiology and Biotechnology - February 6, 2024 Category: Microbiology Source Type: research

Application of sequential design for enhanced l-asparaginase synthesis from Ganoderma australe GPC191
AbstractWith an increasing demand forl-asparaginase in pharmaceutical and food sectors for its cytostatic and acrylamide-reducing qualities, there's a need to discover novel, highly productive enzyme sources with improved pharmacokinetic profiles. Keeping this in mind, the present study aimed at maximizing the potential ofGanoderma australe GPC191 to producel-asparaginase by fermentation medium optimization using statistical validation. Of the 11 physicochemical parameters evaluated under submerged fermentation conditions through one-factor-at-a-time approach and Plackett –Burman design, only four parameters (inoculum lo...
Source: World Journal of Microbiology and Biotechnology - February 1, 2024 Category: Microbiology Source Type: research

Microbial α-L-arabinofuranosidases: diversity, properties, and biotechnological applications
AbstractArabinoxylans (AXs) are hemicellulosic polysaccharides consisting of a linear backbone ofβ-1,4-linked xylose residues branched by high content ofα-L-arabinofuranosyl (Araf) residues along with other side-chain substituents, and are abundantly found in various agricultural crops especially cereals. The efficient bioconversion of AXs into monosaccharides, oligosaccharides and/or other chemicals depends on the synergism of main-chain enzymes and de-branching enzymes. Exo-α-L-arabinofuranosidases (ABFs) catalyze the hydrolysis of terminal non-reducingα-1,2-,α-1,3- orα-1,5- linkedα-L-Araf residues from arabinose-...
Source: World Journal of Microbiology and Biotechnology - January 31, 2024 Category: Microbiology Source Type: research

Manipulation of co-pelletization for Chlorela vulgaris harvest by treatment of Aspergillus niger spore
In this study,Aspergillus niger andChlorella vulgaris were used as the model species of filamentous fungi and microalgae to investigate co-pellets formation usingA. niger spores after by different pH solutions treatment, swelling, snailase treatment. The importance of spore treatments onC. vulgaris harvest in sequence was claimed based on response surface methodology analysis. The pH solutions treatment, swelling, snailase treatment ofA. niger spore contributed 21.0%, 10.5%, 40.7% of harvest ratio ofC. vulgaris respectively, which guided the application of spore treatment into co-pelletization. Treatment of spore was showe...
Source: World Journal of Microbiology and Biotechnology - January 30, 2024 Category: Microbiology Source Type: research

Axenic green microalgae for the treatment of textile effluent and the production of biofuel: a promising sustainable approach
This study illustrates the potential use of TWW as a microalgae feedstock with reduced nutrient supplementation (20% of TWW). Thus, it can be considered a promising feedstock for economical biofuel production.Graphical abstract (Source: World Journal of Microbiology and Biotechnology)
Source: World Journal of Microbiology and Biotechnology - January 29, 2024 Category: Microbiology Source Type: research

Optimizing light regimes for neutral lipid accumulation in Dunaliella salina MCC 43: a study on physiological status and carbon allocation
AbstractDunaliella salina is a favourable source of high lipid feedstock for biofuel and medicinal chemicals. Low biomass output from microalgae is a significant barrier to industrial-scale commercialisation. The current study aimed to determine how photosynthetic efficiency, carbon fixation, macromolecular synthesis, accumulation of neutral lipids, and antioxidative defence (ROS scavenging enzyme activities) ofD. salina cells were affected by different light intensities (LI) (50, 100, 200, and 400  µmol m−2 s−1). The cells when exposed to strong light (400  µmol m−2 s−1) led to reduction in chlorophylla bu...
Source: World Journal of Microbiology and Biotechnology - January 29, 2024 Category: Microbiology Source Type: research

Insight into effects of terbium on cell growth, sporulation and spore properties of Bacillus subtilis
In conclusion, Tb(III) directly or indirectly regulated both the nutrient status of the media and certain metabolic events, which in turn affected most of the properties ofB. subtilis. Compared to the coat-deficient strain, the wild-type strain grew faster and was more tolerant to Tb(III), DPA, and wet heat, which in turn implied that it was more suitable for the recovery of REEs during cultivation. These findings provide fundamental insights for the recovery of rare earths during the culture process using microorganisms. (Source: World Journal of Microbiology and Biotechnology)
Source: World Journal of Microbiology and Biotechnology - January 28, 2024 Category: Microbiology Source Type: research

Diverse roles played by “Pseudomonas fluorescens complex” volatile compounds in their interaction with phytopathogenic microrganims, pests and plants
AbstractPseudomonas fluorescens complex consists of environmental and some human opportunistic pathogenic bacteria. It includes mainly beneficial and few phytopathogenic species that are common inhabitants of soil and plant rhizosphere. Many members of the group are in fact known as effective biocontrol agents of plant pathogens and as plant growth promoters and for these attitudes they are of great interest for biotechnological applications. The antagonistic activity of fluorescentPseudomonas is mainly related to the production of several antibiotic compounds, lytic enzymes, lipopeptides and siderophores. Several volatile...
Source: World Journal of Microbiology and Biotechnology - January 28, 2024 Category: Microbiology Source Type: research

Recent advances in Bacillus-mediated plant growth enhancement: a paradigm shift in redefining crop resilience
AbstractTheBacillus genus has emerged as an important player in modern agriculture, revolutionizing plant growth promotion through recent advances. This review provides a comprehensive overview of the critical roleBacillus species play in boosting plant growth and agricultural sustainability.Bacillus genus bacteria benefit plants in a variety of ways, according to new research. Nitrogen fixation, phosphate solubilization, siderophore production, and the production of growth hormones are examples of these.Bacillus species are also well-known for their ability to act as biocontrol agents, reducing phytopathogens and protecti...
Source: World Journal of Microbiology and Biotechnology - January 23, 2024 Category: Microbiology Source Type: research

Exploiting the synergistic antibacterial activity of shikimic acid and ceftiofur against methicillin-resistant Staphylococcus aureus
This study not only offers a compelling strategy, capitalizing on the synergistic potential of SA and CF, to rapidly address antibiotic resistance but also contributes significantly to the refinement of antimicrobial therapeutic str ategies. (Source: World Journal of Microbiology and Biotechnology)
Source: World Journal of Microbiology and Biotechnology - January 23, 2024 Category: Microbiology Source Type: research

Levilactobacillus brevis MZ384011 and  Levilactobacillus brevis MW362779 can mitigate lead induced hepato-renal damage by regulating visceral dispersion and fecal excretion
AbstractHeavy metal pollution is a global issue. Current study provides evidence on Pb toxicity ameliorative potential and safe nature ofLevilactobacillus brevis MZ384011 (S1) andLevilactobacillus brevis MW362779 (S2), isolated from carnivore gut and human milk, respectively. In a 60-days experiment, the rats were distributed into six groups. G-I, G-V and G-VI were kept on normal diet, while GII-IV were fed on lead nitrate (500  mg/kg) supplemented food, throughout experiment. After confirmation of Pb toxicity in GII-IV at 15th day, S1 was orally administered to G-III and G-V while S2 was given to G-IV and G-VI at a dose ...
Source: World Journal of Microbiology and Biotechnology - January 22, 2024 Category: Microbiology Source Type: research

Study of bacterial population dynamics in seed culture developed for ammonia reduction from synthetic wastewater
In this study the different bacteria involved in total nitrogen removal from the wastewater are enriched for 700  days under anoxic condition. The synthetic wastewater containing 0.382 g/L of ammonium chloride was used. Molecular identification of the bacteria involved in various steps of the nitrogen cycle was carried out based on amplification of functional genes and 16S rRNA gene Polymerase chain reaction followed by DNA sequencing. Change in the abundance of chemolithotrophs was studied using qPCR. The mutual growth of various nitrifiers along with anaerobic bacteria were identified by molecular characterisation of D...
Source: World Journal of Microbiology and Biotechnology - January 22, 2024 Category: Microbiology Source Type: research

Novel primers to identify a wider diversity of butyrate-producing bacteria
AbstractButyrate-producing bacteria are a functionally important part of the intestinal tract flora, and the resulting butyric acid is essential for maintaining host intestinal health, regulating the immune system, and influencing energy metabolism. However, butyrate-producing bacteria have not been defined as a coherent phylogenetic group. They are primarily identified using primers for key genes in the butyrate-producing pathway, and their use has been limited to the Bacillota and Bacteroidetes phyla. To overcome this limitation, we developed functional gene primers able to identify butyrate-producing bacteria through th...
Source: World Journal of Microbiology and Biotechnology - January 22, 2024 Category: Microbiology Source Type: research