World Journal of Microbiology and Biotechnology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Diversity and functional annotation of microorganisms in anaerobic chamber treating nitrate-rich wastewater
AbstractThe vertical flow constructed wetlands (VFCW) for the treatment of domestic wastewater has become a conventional and cost effective treatment system with one of the major disadvantage of elevated nitrate concentrations. The present study makes an effort in providing a new design of anaerobic denitrification unit termed as anaerobic chamber (AC) which  was introduced after two-stage VFCW to remove nitrates from the treated wastewater (WW). The AC provided all the essential conditions of effective denitrification such as anaerobic environment with enough carbon and nitrogen source. To understand the pollutant remova...
Source: World Journal of Microbiology and Biotechnology - September 19, 2023 Category: Microbiology Source Type: research

A mini-review on indigenous microbial biofilm from various wastewater for heavy-metal removal - new trends
AbstractBiofilm, as a form of the microbial community in nature, represents an evolutionary adaptation to the influence of various environmental conditions. In nature, the largest number of microorganisms occur in the form of multispecies biofilms. The ability of microorganisms to form a biofilm is one of the reasons for antibiotic resistance. The creation of biofilms resistant to various contaminants, on the other hand, improves the biological treatment process in wastewater treatment plants. Heavy metals cannot be degraded, but they can be transformed into non-reactive and less toxic forms. In this process, microorganism...
Source: World Journal of Microbiology and Biotechnology - September 16, 2023 Category: Microbiology Source Type: research

Evaluating β-cryptoxanthin antioxidant properties against ROS-induced macromolecular damages and determining its photo-stability and in-vitro SPF
This study investigated the antioxidant property of β-cryptoxanthin (β-CRX) extracted from Kocuria marina DAGII and its protective effect against macromolecular damages by generating ROS via two models: UV radiation and the Fenton reaction. β-cryptoxanthin exhibited the highest scavenging activity towards hydrogen peroxide radicals with an IC50 value of 38.30  ± 1.13 μg/ml, favoring the hydrogen atom transfer mechanism. The total antioxidant capacity value of 872.0101 ± 1.84 μg BHT/mg β-CRX indicated the cumulative ROS scavenging ability of β-cryptoxanthin. β-cryptoxanthin could protect against ROS-in...
Source: World Journal of Microbiology and Biotechnology - September 16, 2023 Category: Microbiology Source Type: research

Isolation and characterization of two glycolipopeptids biosurfactants produced by a Lactiplantibacillus plantarum OL5 strain isolated from green olive curing water
AbstractMicrobial surfactants are natural amphiphilic compounds  with high surface activities and emulsifying properties. Due to their structural diversity, low toxicity, biodegradability, and chemical stability in different conditions, these molecules are potential substitutes for chemical surfactants; their interest has grown significantly over the last decad e. The current study focuses on the isolation, identification, and characterization of a lactic acid bacteria that produce two forms of biosurfactants. The OL5 strain was isolated from green olive fermentation and identified using MALDI/TOF and DNAr16S amplificatio...
Source: World Journal of Microbiology and Biotechnology - September 16, 2023 Category: Microbiology Source Type: research

The ester production capacity of Pichia kudriavzevii based on functional annotation of genes
This study focused on the yeast strainPichia kudriavzevii, renowned for its high capacity to produce esters. Whole genome sequences were annotated and analyzed using the GO, KEGG, KOG, CAZy, and Pfam databases to determine the genetic basis underly the enhanced ester production capacity. Results showed thatP. kudriavzevii gene function was concentrated in biosynthetic capacity, metabolic capacity, amino acid translocation capacity, glycoside hydrolysis capacity and transfer capacity. Additionally, acyltransferase and kinase were predicted as active sites contributing toP. kudriavzevii high ester production. We further comp...
Source: World Journal of Microbiology and Biotechnology - September 15, 2023 Category: Microbiology Source Type: research

Expression profiling and characterization of key RGA involved in lentil Fusarium wilt Race 5 resistance
AbstractFusarium wilt is a major threat to lentil production in India and worldwide. The presence of evolving virulent races has imposed the necessity of reliable management practices including breeding for resistance using unexplored germplasms. The magnitude of resistance by the plant is determined by rapid recognition of the pathogen and induction of defence genes. Resistance gene analogues have been key factors involved in the recognition and induction of defence response. In the present study, the expression of key RGA previously cloned was determined in three resistant accessions (L65, L83 and L90) and a susceptible ...
Source: World Journal of Microbiology and Biotechnology - September 15, 2023 Category: Microbiology Source Type: research

High NaCl concentrations induce the resistance to thermal denaturation of an extremely halotolerant (salt-activated) β-mannanase from Bacillus velezensis H1
This study aims to report the first purification and characterization of ManH1, an extremely halotolerant β-mannanase from the halotolerantB. velezensis strain H1. Electrospray ionization quadrupole time-of-flight mass spectrometry (ESI-Q-TOF-MS) analysis revealed a single major peak with a molecular mass of 37.8  kDa demonstrating its purity. The purified enzyme showed a good thermostability as no activity was lost after a 48 h incubation under optimal conditions of 50 °C and pH 5.5. The enzyme’s salt activation nature was revealed when its maximum activity was obtained in the presence of 4 M NaCl,  it doubled co...
Source: World Journal of Microbiology and Biotechnology - September 11, 2023 Category: Microbiology Source Type: research

Fungal pectinases: an insight into production, innovations and applications
AbstractThe fungal system holds morphological plasticity and metabolic versatility which makes it unique. Fungal habitat ranges from the Arctic region to the fertile mainland, including tropical rainforests, and temperate deserts. They possess a wide range of lifestyles behaving as saprophytic, parasitic, opportunistic, and obligate symbionts. These eukaryotic microbes can survive any living condition and adapt to behave as extremophiles, mesophiles, thermophiles, or even psychrophile organisms. This behaviour has been exploited to yield microbial enzymes which can survive in extreme environments. The cost-effective produc...
Source: World Journal of Microbiology and Biotechnology - September 11, 2023 Category: Microbiology Source Type: research

New progress in the identifying regulatory factors of exopolysaccharide synthesis in lactic acid bacteria
AbstractThe exopolysaccharides (EPSs) of lactic acid bacteria (LAB) have presented various bioactivities and beneficial characteristics, rendering their vast commercial value and attracting a broad interest of researchers. The diversity of EPS structures contributes to the changes of EPS functions. However, the low yield of EPS of LAB has severely limited these biopolymers ’ comprehensive studies and applications in different areas, such as functional food, health and medicine fields. The clarification of biosynthesis mechanism of EPS will accelerate the synthesis and reconstruction of EPS. In recent years, with the deve...
Source: World Journal of Microbiology and Biotechnology - September 9, 2023 Category: Microbiology Source Type: research

Towards an understanding of the enzymatic degradation of complex plant mannan structures
AbstractPlant cell walls are composed of a heterogeneous mixture of polysaccharides that require several different enzymes to degrade. These enzymes are important for a variety of biotechnological processes, from biofuel production to food processing. Several classical mannanolytic enzyme functions of glycoside hydrolases (GH), such as β-mannanase, β-mannosidase and α-galactosidase activities, are helpful for efficient mannan hydrolysis. In this light, we bring three enzymes into the model of mannan degradation that have received little or no attention. By linking their three-dimensional structures and substrate specifi...
Source: World Journal of Microbiology and Biotechnology - September 9, 2023 Category: Microbiology Source Type: research

Musico-bioremediation of seafood canning wastewater by Yarrowia lipolytica
AbstractDue to the lack of water resources and the harmful effects of wastewater on environment and human health, treatment of wastewater becomes necessary. The present study explored the effect of musical sounds on the biological treatment of seafood canning wastewater by usingYarrowia lipolytica. Our results showed that low frequency (21  Hz to 1356 Hz) and high frequency (21 Hz to 16,214 Hz) musical sounds stimulated the growth ofY. lipolytica and increased the polluant removal efficiency. Such treatment decreased significantly the chemical oxygen demand (COD) and salinity as well as the color of this wastewater. Our ...
Source: World Journal of Microbiology and Biotechnology - September 9, 2023 Category: Microbiology Source Type: research

Mycobacterium tuberculosis and its clever approaches to escape the deadly macrophage
AbstractMycobacterium tuberculosis (Mt.b), a deadly disease causer, is a facultative parasite. This microorganism has developed several methods to defend itself, once internalized within specialised vacuoles in the macrophages. A wide array of receptors like the complement receptor mannose receptors, scavenger receptor assists the entry of the microbe within the phagocytic macrophages. However,Mt.b is clever enough to protect itself from the hostile environment of the macrophage thereby prevailing within it. The microbe can efficiently inhibit processes like phagosome-lysosome fusion, acidification of phagosomes, release o...
Source: World Journal of Microbiology and Biotechnology - September 5, 2023 Category: Microbiology Source Type: research

Biofilm formation by agave epiphytic lactic acid bacteria fed with agave fructans
AbstractThe aim of this work was to find out if biofilms can be made by lactic acid bacteria (LAB) isolated from agave plants using agave fructans as sole carbohydrate substrates or if it was necessary to use fructose as a breakdown product of such polymers. This is part of a research project geared to develop industrial lactic acid production from agave fructans, an abundant raw material in Mexico ’s agave plantations. Present results showed that nine strains of LAB isolated fromAgave salmiana and belonging to genusLacticaseibacillus andEnterococcus produced exopolysaccharides directly from agave fructans to a greater e...
Source: World Journal of Microbiology and Biotechnology - September 5, 2023 Category: Microbiology Source Type: research

Comparative metabolomic and transcriptomic analysis of Saccharomyces cerevisiae W303a and CEN.PK2-1C
In this study, metabolomics and transcriptomics were applied to analyze the differential metabolites and differential expression genes (DEGs) between W303a and CEN.PK2-1C cultured in YPD and SD media. The growth rate of W303a in YPD medium was the lowest compared with other groups. When cultured in YPD medium, CEN.PK2-1C produced more phenylalanine than W303a; when cultured in SD medium, W303a produced more phospholipids than CEN.PK2-1C. Transcriptomic analysis revealed that 19 out of 22 genes in glycolysis pathway were expressed at higher levels in CEN.PK2-1C than that in W303a no matter which media were used, and three k...
Source: World Journal of Microbiology and Biotechnology - September 4, 2023 Category: Microbiology Source Type: research

Space exposure enhanced pectin-degrading enzymes expression and activity in Aspergillus costaricaensis
This study investigated the effects of space exposure on the ability ofAspergillus costaricaensis, a filamentous fungus isolated from rotten orange peel, to degrade pectin. These fungal spores were carried into space by the Long March 5B carrier rocket and exposed to cosmic radiation for 79  h. After the flight, these spores were resuscitated, and then the growing strains were screened with pectin as the sole carbon source, and the pectinase activity was evaluated. A mutant with increased biomass accumulation ability and pectin-degrading activity compared to the ground control strain was obtained. Comparative transcriptom...
Source: World Journal of Microbiology and Biotechnology - September 2, 2023 Category: Microbiology Source Type: research