World Journal of Microbiology and Biotechnology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Bilobetin attenuates Staphylococcus aureus virulence by targeting Von Willebrand factor-binding protein and staphylocoagulase
AbstractStaphylococcus aureus (S. aureus) induces a variety of infectious diseases in humans and animals and is responsible for hospital- and community-acquired infections. The aim of this study was to investigate how bilobetin, a natural compound, attenuatesS. aureus virulence by inhibiting two key virulence factors, von Willebrand factor-binding protein (vWbp) and staphylocoagulase (Coa). The results showed that bilobetin inhibited Coa- or vWbp-induced coagulation without affectingS. aureus proliferation. The Western blotting and fluorescence quenching assays indicated that bilobetin did not affect the expression of vWbp...
Source: World Journal of Microbiology and Biotechnology - October 27, 2023 Category: Microbiology Source Type: research

Recombinant Prevotella melaninogenica α-1,3 glucanase and Capnocytophaga ochracea α-1,6 glucanase as enzymatic tools for in vitro degradation of S. mutans biofilms
AbstractDental biofilms represent a serious oral health problem playing a key role in the development of caries and other oral diseases. In the present work, we cloned and expressed inE. coli two glucanases,Prevotella melaninogenica mutanase (PmGH87) andCapnocytophaga ochracea dextranase (CoGH66), and characterized them biochemically and biophysically. Their three-dimensional structures were elucidated and discussed. Furthermore, we tested the capacity of the enzymes to hydrolyze mutan and dextran to prevent formation ofStreptococcus mutans biofilms, as well as to degrade pre- formed biofilms in low and abundant sugar cond...
Source: World Journal of Microbiology and Biotechnology - October 26, 2023 Category: Microbiology Source Type: research

Comparative genomic analysis of Mycoplasma related to cell culture for infB gene-based loop-mediated isothermal amplification
In conclusion, we present a methodology utilizing theinfB gene-based LAMP assay to detect three of the six most prevalentMycoplasma species in cell culture.Graphical abstract (Source: World Journal of Microbiology and Biotechnology)
Source: World Journal of Microbiology and Biotechnology - October 25, 2023 Category: Microbiology Source Type: research

Inhibitory effects of hinokitiol on the development and pathogenicity of Colletotrichum gloeosporioides
AbstractPostharvest anthracnose of mango fruit caused byColletotrichum gloeosporioides is a devastating fungal disease, which causes tremendous quality deterioration and economic losses. Hinokitiol, an environmentally friendly natural compound, is effective in controlling a variety of postharvest fungal diseases. However, there is still a lack of research on the inhibitory effect of hinokitiol onC. gloeosporioides and its possible modes of action. In the present study, the activity of hinokitiol againstC. gloeosporioides and its potential mechanisms involved have been investigated. We found that hinokitiol treatment could ...
Source: World Journal of Microbiology and Biotechnology - October 25, 2023 Category: Microbiology Source Type: research

Campylobacter jejuni and Campylobacter coli in human stool samples: antibiotic resistance profiles, putative virulence determinants and molecular characterization of the isolates
In conclusion, this was a very comprehensive study revealing the presence of antibiotic-resistantC. jejuni andC. coli with various virulence genes in patients admitted to a university hospital with acute gastroenteritis. This is of utmost significance for public health. Since campylobacteria are foodborne, zoonotic pathogens and transmission occurs mostly through food. People should have detailed information about the transmission routes of campylobacteria and risky foods. In addition, staff, food processors and caterers, should be trained in hygiene. (Source: World Journal of Microbiology and Biotechnology)
Source: World Journal of Microbiology and Biotechnology - October 24, 2023 Category: Microbiology Source Type: research

Effects of rotation and Bacillus on the changes of continuous cropping soil fungal communities in American ginseng
This study suggested that compared to probioticBacillus, crop rotation can significantly affect soil fungal community structure, especially the enrichment of specific potentially beneficial fungal species. Our findings provide a scientific basis for understanding the dynamic changes of fungal communities and soil properties with continuous cropping obstacle of American ginseng in initial stage of soil improvement. (Source: World Journal of Microbiology and Biotechnology)
Source: World Journal of Microbiology and Biotechnology - October 24, 2023 Category: Microbiology Source Type: research

Engineering of formate dehydrogenase for improving conversion potential of carbon dioxide to formate
AbstractFormate dehydrogenase (FDH) is aD-2-hydroxy acid dehydrogenase, which can reversibly reduce CO2 to formate and thus act as non-photosynthetic CO2 reductase. In order to increase catalytic efficiency of formate dehydrogenase for CO2 reduction, two mutants V328I/F285W and V354G/F285W were obtained of which reduction activity was about two times more than the parentCbFDHM2, and the formate production from CO2 catalyzed by mutants were 2.9 and 2.7-fold higher than that of the parentCbFDHM2. The mutants had greater potential in CO2 reduction. The optimal temperature for V328I/F285W and V354G/F285W was 55  °C, and they...
Source: World Journal of Microbiology and Biotechnology - October 21, 2023 Category: Microbiology Source Type: research

Molecular characterization of vermicompost-derived IAA-releasing bacterial isolates and assessment of their impact on the root improvement of banana during primary hardening
AbstractThe hardening step of micropropagation is crucial to make the in vitro raised plants mature and further enhancing their survivability in the external environment. Auxin regulates various root physiological parameters in plant systems. Therefore, the present study aimed to assess the impact of three vermicompost-derived IAA-releasing microbial strains, designated S1, S2, and S3, as biofertilizers on in vitro raised banana plantlets during primary hardening. The High-Performance Thin-Layer Chromatography (HPTLC) analysis of these strains revealed a higher IAA content for S1 and S2 than that of S3 after 144  h of inc...
Source: World Journal of Microbiology and Biotechnology - October 21, 2023 Category: Microbiology Source Type: research

Analysis of the bacterial and fungal populations in South African sorghum beer (umqombothi) using full-length 16S rRNA amplicon sequencing
AbstractThere is a need to profile microorganisms which exist pre-and-post-production ofumqombothi, to understand its microbial diversity and the interactions which subsequently influence the final product. Thus, this study sought to determine the relative microbial abundance inumqombothi and predict the functional pathways of bacterial and fungal microbiota present. Full-length bacterial 16S rRNA and internal transcribed spacer (ITS) gene sequencing using PacBio single-molecule, real-time (SMRT) technology was used to assess the microbial compositions. PICRUSt2 was adopted to infer microbial functional differences. A mixt...
Source: World Journal of Microbiology and Biotechnology - October 21, 2023 Category: Microbiology Source Type: research

Study of growth-improving and sporophore-inducing endobacteria isolated from Pleurotus pulmonarius
AbstractSeveralPleurotus species (oyster mushrooms) are commercially cultivated in India owing to the favorable tropical agro-climatic conditions. However, there are only a few studies on the microbiome of mushrooms, especially oyster mushrooms. The aim of this study was to assess the effect of endobacteria on mycelial growth, spawning, sporophore development, and proximate composition ofP. pulmonarius. We isolated several bacterial strains from the sporophores ofP. pulmonarius and assessed the in vitro production of indole acetic acid, ammonia, and siderophores. The selected bacteria were individually supplemented with sp...
Source: World Journal of Microbiology and Biotechnology - October 20, 2023 Category: Microbiology Source Type: research

Implication of Mn-cofactored superoxide dismutase in the tolerance of swarmer Pseudomonas aeruginosa to polymixin, ciprofloxacin and meropenem antibiotics
AbstractThe protective role of superoxide dismutase (Sod) against oxidative stress, resulting from the common antibiotic pathway of action, has been studied in the wild type and mutant strains of swarmerPseudomonas aeruginosa, lacking Cytosolic Mn-Sod (sodM), Fe-Sod (sodB) or both Sods (sodMB).Our results showed that inactivation ofsodB genes leads to significant motility defects and tolerance to meropenem. This resistance is correlated with a greater membrane unsaturation as well as an effective intervention of Mn-Sod isoform, in antibiotic tolerance.Moreover, loss of Mn-Sod insodM mutant, leads to polymixin intolerance a...
Source: World Journal of Microbiology and Biotechnology - October 19, 2023 Category: Microbiology Source Type: research

Computational analysis of carboxylesterase genes and proteins in non-pathogenic food bacterium Alicyclobacillus acidocaldarius: insights from proteogenomics
This study presents the computational proteogenomics report on the carboxylesterase (CE) enzyme inA. acidocaldarius, shedding light on structural and evolutional of CEs from this bacterium. Our analysis revealed that the average molecular weight of CEs inA. acidocaldarius was 41  kDa, with an isoelectric point around 5. The amino acid composition favored negative amino acids over positive ones. The aliphatic index and hydropathicity were approximately 88 and − 0.15, respectively. While the protein sequence showed no disulfide bonds in the CEs’ structure, the presence of Cys amino acids was observed in the structure ...
Source: World Journal of Microbiology and Biotechnology - October 19, 2023 Category: Microbiology Source Type: research

Exploring the hidden treasures of Nitella hyalina: a comprehensive study on its biological compounds, nutritional profile, and unveiling its antimicrobial, antioxidative, and hypoglycemic properties
In conclusion, the macroalgae demonstrated potency concerning antioxidant, antimicrobial, and hypoglycemic properties. (Source: World Journal of Microbiology and Biotechnology)
Source: World Journal of Microbiology and Biotechnology - October 16, 2023 Category: Microbiology Source Type: research

Evaluating the potential of semi-continuous itaconic acid fermentation by Aspergillus terreus: operational profile and experiences
AbstractItaconic acid is an important bio-based chemical. The present study aims to evaluate the applicability of semi-continuous fermentation technique for itaconic acid production byAspergillus terreus. The fermentation is planned to be connected with bipolar membrane electrodialysis unit for acid recovery. This process allows the reuse of residual glucose from the effluent. Our particular attention was focused on the effect of glucose concentration. Two different glucose supplementation strategies were tested: constant glucose concentration in the refilling medium and adjusted glucose concentration in order to maintain ...
Source: World Journal of Microbiology and Biotechnology - October 16, 2023 Category: Microbiology Source Type: research

Recent advances in enzymatic properties, preparation methods, and functions of glycoside hydrolase from Bifidobacterium: a review
AbstractBifidobacterium is a major probiotic of intestinal gut flora and exerts many physiological activities, and it is widely applied in the fields of food and medicine. As an important part ofBifidobacterium, glycoside hydrolase plays a role in its physiological activity. With the continuous development and improvement of genetic engineering technology, research on this type of enzyme will play a crucial role in promoting the further development ofBifidobacterium in the field of probiotics. In this review, the preparation methods, enzymatic properties, and functions of glycoside hydrolase extracted fromBifidobacterium a...
Source: World Journal of Microbiology and Biotechnology - October 16, 2023 Category: Microbiology Source Type: research