World Journal of Microbiology and Biotechnology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Phenotypic, molecular, and symbiotic characterization of the rhizobial symbionts isolated from Acacia saligna grown in different regions in Morocco: a multivariate approach
AbstractThe introduced speciesAcacia saligna is a very promiscuous host as it can be efficiently nodulated with a wide range diversity of rhizobia taxa, including both fast and slow-growing strains. Fourteen nitrogen (N)-fixing bacteria were isolated from root nodules of wildAcacia saligna growing in distinct geographic locations in Morocco and were examined for their symbiotic efficiency and phenotypic properties. Multivariate tools, such as principal component analysis (PCA) and hierarchical clustering analysis (HCA), were used to study the correlation between phenotypic and symbiotic variables and discriminate and descr...
Source: World Journal of Microbiology and Biotechnology - October 16, 2023 Category: Microbiology Source Type: research

Bamboo polysaccharides elicit hypocrellin A biosynthesis of a bambusicolous fungus Shiraia sp. S9
This study is the first report of the enhancement of HA production in mycelium culture ofShiraia sp. S9 by the polysaccharides from its host bamboo which serve as a strong elicitor. A purified bamboo polysaccharide (BPSE) with an average molecular weight of 34.2  kDa was found to be the most effective elicitor to enhance fungal HA production and characterized as a polysaccharide fraction mainly composed of arabinose and galactose (53.7: 36.9). When BPSE was added to the culture at 10 mg/L on day 3, the highest HA production of 422.8 mg/L was achieved on day 8, which was about 4.0-fold of the control. BPSE changed the ge...
Source: World Journal of Microbiology and Biotechnology - October 13, 2023 Category: Microbiology Source Type: research

Purification of Galacto-oligosaccharide (GOS) by fermentation with Kluyveromyces lactis and Interaction between GOS and casein under simulated acidic fermentation conditions
This study was aimed to provide a method to to purify GOS by yeat fermentation and explore the interaction between GOS and CAS with a view for expanding the prospects of GOS application in the food industry. The crude GOS(25.70  g/L) was purified in this study using the fermentation method withKluyveromyces lactis CICC 1773. Optimal conditions for purification with the yeast were 75  g/L of the yeast inoculation rate and 50 g/L of the initial crude GOS concentration for 12 h of incubation. After removing ethanol produced by yeast by low-temperature distillation, GOS content could reach 90.17%. A study of the interactio...
Source: World Journal of Microbiology and Biotechnology - October 13, 2023 Category: Microbiology Source Type: research

Surfactin inhibits Fusarium graminearum by accumulating intracellular ROS and inducing apoptosis mechanisms
This study demonstrates the potential of surfactin as an antifungal agent for FHB biocontrol, provides a new perspective on the antifungal mechanism of surfactin against filamentous fungi, and contributes to the application of surfactin-producing microbes in the biocontrol of plant diseases. (Source: World Journal of Microbiology and Biotechnology)
Source: World Journal of Microbiology and Biotechnology - October 12, 2023 Category: Microbiology Source Type: research

Study of peripheral domains in structure –function of isocitrate lyase (ICL) from Pseudomonas aeruginosa
This study investigates the role of domain II, the extended motif, and the carboxyl-terminus (C-ICL) and amino-terminus (N-ICL) regions in the function of the PaICL enzyme, also as their involvement in the virulence ofP. aeruginosa PAO1. Deletion of domain II and the extended motif results in enzyme inactivation and structural instability of the enzyme. The His6-tag fusion at the C-ICL protein produced a less efficient enzyme than fusion at the N-ICL, but without affecting the acetate assimilation or virulence. The PaICL homotetrameric structure of the enzyme was more stable in the N-His6-ICL than in the C-His6-ICL, sugges...
Source: World Journal of Microbiology and Biotechnology - October 12, 2023 Category: Microbiology Source Type: research

Chemical extraction, characterization, and inspection of the antimicrobial and antibiofilm activities of shrimp chitosan against foodborne fungi and bacteria
AbstractNowadays, the exploitation of biopolymers in the industrial sector has become a trend. Chitosan is considered one of the most investigated biopolymers due to its abundance and antibacterial, antifungal, and antibiofilm activities. In this work, chitosan was chemically extracted from shrimp shells. Solutions of HCl 1  M, NaOH 4 M, and NaOH 15 M were used for the demineralization, deproteinization, and deacetylation process, respectively. The utilized methods of characterization (FTIR,1 H NMR,13 C NMR, and SEC-MALS) revealed that the obtained chitosan has a moderate degree of deacetylation and low molecular weig...
Source: World Journal of Microbiology and Biotechnology - October 12, 2023 Category: Microbiology Source Type: research

Chromosomal gene of hybrid multisensor histidine kinase is involved in motility regulation in the rhizobacterium Azospirillum baldaniorum Sp245 under mechanical and water stress
In this study, we obtained theA. baldaniorum Sp245 mutant for the AZOBR_150176 gene, which encodes the TCS of the hybrid histidine kinase/response sensory regulator (HSHK-RR). Inactivation of this gene affected bacterial morphology and motility. In mutant Sp245-HSHKΔRR-Km, the cells were still able to synthesize a functioning polar flagellum (Fla), were shorter than those of strain Sp245, and were impaired in aerotaxis, elaboration of inducible lateral flagella (Laf), and motility in semiliquid media. The mutant showed decreased transcription of the genes encoding the proteins of the secretion apparatus, which ensures the...
Source: World Journal of Microbiology and Biotechnology - October 10, 2023 Category: Microbiology Source Type: research

Ultra-high-depth macrogenomic sequencing revealed differences in microbial composition and function between high temperature and medium –high temperature Daqu
AbstractComplex microorganisms inDaqu of different temperatures play a vital role in the taste, flavor and quality ofBaijiu during fermentation. However, understanding the functional diversity of the whole microbial community between theDaqus of two different temperatures (high temperatureDaqu, HD and medium –high temperatureDaqu, MD) remains a major challenge. Here, a systematic study of the microbial diversity, functions as well as physiological and biochemical indexes ofDaqu are described. The results revealed that theDaqu exhibited unique characteristics. In particular, the diversity of microorganisms in HD and MD wa...
Source: World Journal of Microbiology and Biotechnology - October 10, 2023 Category: Microbiology Source Type: research

Pathognomonic features of Pasteurella multocida isolates among various avian species in Sharkia Governorate, Egypt
In conclusion, this study presents a comprehensive picture of the risk factors in correlation to the pathognomonic characteristics ofP. multocida infection in poultry sectors to help in developing more effective strategies for prevention and control. (Source: World Journal of Microbiology and Biotechnology)
Source: World Journal of Microbiology and Biotechnology - October 9, 2023 Category: Microbiology Source Type: research

Cell free extract-mediated biogenic synthesis of ZnONPs and their application with kanamycin as a bactericidal combination
AbstractAntimicrobial resistance (AMR) is a main public health issue and a challenge for the scientific community all over the globe. Hence, there is a burning need to build new bactericides that resist the AMR. The ZnONPs were produced by cell free extract of mint (Mentha piperita L.) leaves. Antibiotics that are ineffective against resistant bacteria likeEscherichia coli andStaphylococcus aureus were treated. The antibiotics were first screened, and then antibacterial activity was checked by disk diffusion, and MIC of Mp-ZnONPs individually and using Kanamycin (KAN) were determined against these pathogens by broth microd...
Source: World Journal of Microbiology and Biotechnology - October 9, 2023 Category: Microbiology Source Type: research

Selective pks+  Escherichia coli strains induce cell cycle arrest and apoptosis in colon cancer cell line
Abstractpks+  Escherichia coli (E. coli) triggers genomic instability in normal colon cells which leads to colorectal cancer (CRC) tumorigenesis. Previously, we reported a significant presentation ofpks+  E. coli strains in CRC patients ’ biopsies as compared to healthy cohorts. In this work, using an in vitro infection model, we further explored the ability of these strains in modulating cell cycle arrest and activation of apoptotic mediators in both primary colon epithelial cells (PCE) and CRC cells (HCT-116). Sixteen strains, of which eight tumours and the matching non-malignant tissues, respectively, from eightp...
Source: World Journal of Microbiology and Biotechnology - October 6, 2023 Category: Microbiology Source Type: research

DeoR regulates lincomycin production in Streptomyces lincolnensis
This study explored the function of DeoR (SLINC_8027) inStreptomyces lincolnensis. Deletion ofdeoR in NRRL 2936 led to an increase in cell growth. The lincomycin production of thedeoR deleted strain ΔdeoR was 3.4-fold higher than that of the wild strain. This trait can be recovered to a certain extent in thedeoR complemented strain ΔdeoR::pdeoR. According to qRT-PCR analysis, DeoR inhibited the transcription of all detectable genes in the lincomycin biosynthesis cluster and repressed the expression ofglnR,bldD, andSLCG_Lrp, which encode regulators outside the cluster. DeoR also inhibited the transcription of itself, as r...
Source: World Journal of Microbiology and Biotechnology - October 6, 2023 Category: Microbiology Source Type: research

Biodiversity and biotechnological properties of lactic acid bacteria isolated from traditional Moroccan sourdoughs
AbstractThe present study aimed at characterizing lactic acid bacteria (LAB) strains isolated from traditional sourdoughs collected in different regions of Morocco. Isolated strains were firstly identified using Gram staining and catalase reaction test. Presumptive LAB strains were then checked for various phenotypical properties including growth at 45  °C, resistance to NaCl, enzyme production, acidification capacity, diacetyl and exopolysaccharide (EPS) production, and antifungal activity. Finally, selected LAB strains were identified using 16S rDNA sequencing. Results showed that 32.1% of the isolates were thermophili...
Source: World Journal of Microbiology and Biotechnology - October 6, 2023 Category: Microbiology Source Type: research

Unveiling novel insights into haloarchaea (Halolamina pelagica CDK2) for alleviation of drought stress in wheat
AbstractPlant growth promoting microorganisms have various implications for plant growth and drought stress alleviation; however, the roles of archaea have not been explored in detail. Herein, present study was aimed for elucidating potential of haloarchaea (Halolamina pelagica CDK2) on plant growth under drought stress. Results showed that haloarchaea inoculated wheat plants exhibited significant improvement in total chlorophyll (100%) and relative water content (30.66%) compared to the uninoculated water-stressed control (30% FC). The total root length (2.20-fold), projected area (1.60-fold), surface area (1.52-fold), nu...
Source: World Journal of Microbiology and Biotechnology - October 4, 2023 Category: Microbiology Source Type: research

Production of high-value oxidative enzymes by Cyathus bulleri on agricultural and agri-food wastes for application in the textile sector
AbstractLigninolytic and other oxidative enzymes have emerged as promising biocatalysts in several industries. Since their production at a low cost is necessary for any large-scale application, we demonstrate the use of rice bran (RB), an agricultural waste and agri-food wastes such as potato peelings (PP), banana peelings (BP), and green pea peelings (GPP) for their production. High activity of laccase (12 U/ml), manganese peroxidase (16.11  ± 1.43 U/ml), and aryl alcohol oxidase (1.25 U/ml) was obtained on the PP on the 12th day of growth and ~ 6 U/ml of lytic polysaccharide monooxygenase was obtained on the 14t...
Source: World Journal of Microbiology and Biotechnology - October 4, 2023 Category: Microbiology Source Type: research