DeoR regulates lincomycin production in Streptomyces lincolnensis

This study explored the function of DeoR (SLINC_8027) inStreptomyces lincolnensis. Deletion ofdeoR in NRRL 2936 led to an increase in cell growth. The lincomycin production of thedeoR deleted strain ΔdeoR was 3.4-fold higher than that of the wild strain. This trait can be recovered to a certain extent in thedeoR complemented strain ΔdeoR::pdeoR. According to qRT-PCR analysis, DeoR inhibited the transcription of all detectable genes in the lincomycin biosynthesis cluster and repressed the expression ofglnR,bldD, andSLCG_Lrp, which encode regulators outside the cluster. DeoR also inhibited the transcription of itself, as revealed by the XylE reporter. Furthermore, we demonstrated that DeoR bound directly to the promoter region ofdeoR,lmbA,lmbC-D,lmbJ-K,lmrA,lmrC,glnR, andSLCG_Lrp, by recognizing the 5 ʼ-CGATCR-3’ motif. This study found that versatile regulatory factor DeoR negatively regulates lincomycin biosynthesis and cellular growth inS. lincolnensis, which expanded the regulatory network of lincomycin biosynthesis.
Source: World Journal of Microbiology and Biotechnology - Category: Microbiology Source Type: research