World Journal of Microbiology and Biotechnology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Improvement of the production and quality of Cordyceps javanica conidia for the control of Diaphorina citri adults
This study aimed to evaluate the use of palm kernel meal (PKM) in the traditional solid-state fermentation system to improve the production and quality ofCordyceps javanica conidia. The impact of PKM was determined by measuring conidia yield, viability, hydrophobicity, shelf life, and conidia pathogenicity againstDiaphorina citri adults. By supplementing rice grains with 5% palm kernel meal increased the conidial yield by up to 40%, without compromising conidia viability and hydrophobicity. In addition, conidia caused higher levels of mortality by mycosis againstD. citri adults (90%), relative to conidia harvested from ric...
Source: World Journal of Microbiology and Biotechnology - February 29, 2024 Category: Microbiology Source Type: research

Establishing a pulmonary aspergillus fumigatus infection diagnostic platform based on RPA-CRISPR-Cas12a
In this study, we devised a diagnostic platform harnessing a combination of recombinase polymerase amplification (RPA) and the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas12a system. Notably, this platform obviates the need for intricate equipment and finds utility in diverse settings. Two result display methods were incorporated in this investigation: the RPA-Cas12a-fluorescence method and the RPA-Cas12a-LFS (lateral flow strip). Upon validation, both display platforms exhibited no instances of cross-reactivity, with seven additional types of fungal pathogens responsible for respiratory infection...
Source: World Journal of Microbiology and Biotechnology - February 29, 2024 Category: Microbiology Source Type: research

Metagenomics analysis of water samples collected from the Yamuna River of Agra city, India
In this study, Yamuna River water samples were collected from three different sites in Agra city during the monsoon, summer, and winter seasons. The physico-chemical parameters were estimated along with heavy metals. In physico-chemical parameter, the values found were mostly above the permissible limits. The results water samples contain high levels of cadmium, chromium, lead, and nickel above the desirable levels in most cases. The metagenomic analysis revealed that Proteobacteria, Bacteroidetes, Verrucomicrobia, Actinobacteria, and Planctobacteria were the most abundant phyla with a relative abundance of 61%, 9.34%, 5.2...
Source: World Journal of Microbiology and Biotechnology - February 29, 2024 Category: Microbiology Source Type: research

Transplantation of soil from organic field confers disease suppressive ability to conducive soil
This study aimed at transforming a disease-conducive soil from a conventional field into disease-suppressive soil by inoculating soil from an organic field previously established as “disease-suppressive”. The effectiveness of the transformed soil was established with the model plant wheat (Triticum aestivum) grown under natural conditions, with regard to its potential in inhibiting fungal phytopathogens,Rhizoctonia solani andFusarium oxysporum. The conducive soil inoculated with the disease-suppressive soil performed better than the control conducive soil in terms of reduced disease severity in plants, improved soil nu...
Source: World Journal of Microbiology and Biotechnology - February 28, 2024 Category: Microbiology Source Type: research

Habitat shapes the gut microbiome diversity of Malayan tigers (Panthera tigris jacksoni) as revealed through metabarcoding 16S rRNA profiling
AbstractThe gut microbiome refers to the microorganism community living within the digestive tract. The environment plays a crucial role in shaping the gut microbiome composition of animals. The gut microbiome influences the health and behavior of animals, including the critically endangered Malayan tiger  (Panthera tigris jacksoni). However, the gut microbiome composition of Malayan tigers, especially those living in their natural habitats, remains poorly understood. To address this knowledge gap, we used next-generation sequencing DNA metabarcoding techniques to analyze the gut microbiome of wild Malayan tigers using fe...
Source: World Journal of Microbiology and Biotechnology - February 28, 2024 Category: Microbiology Source Type: research

Methane production and lignocellulosic degradation of wastes from rice, corn and sugarcane by natural anaerobic fungi-methanogens co-culture
AbstractBiomass from agriculture, forestry, and urban wastes is a potential renewable organic resource for energy generation. Many investigations have demonstrated that anaerobic fungi and methanogens could be co-cultured to degrade lignocellulose for methane generation. Thus, this study aimed to evaluate the effect of natural anaerobic fungi-methanogens co-culture on the methane production and lignocellulosic degradation of wastes from rice, corn and sugarcane. Hu sheep rumen digesta was used to develop a natural anaerobic fungi-methanogen co-culture. The substrates were rice straw (RS), rich husk (RH), corn stover (CS), ...
Source: World Journal of Microbiology and Biotechnology - February 27, 2024 Category: Microbiology Source Type: research

Bacillus species as tools for biocontrol of plant diseases: A meta-analysis of twenty-two years of research, 2000 –2021
AbstractThe traditional way of dealing with plant diseases has been the use of chemical products, but these harm the environment and are incompatible with the global effort for sustainable development. The use ofBacillus and related species in the biological control of plant diseases is a trend in green agriculture. Many studies report the positive effect of these bacteria, but a synthesis is still necessary. So, the objective of this work is to perform a meta-analysis ofBacillus biocontrol potential and identify factors that drive its efficacy. Data were compiled from articles published in journals listed in two of the ma...
Source: World Journal of Microbiology and Biotechnology - February 27, 2024 Category: Microbiology Source Type: research

Hydrogel capsules as new delivery system for Trichoderma koningiopsis Th003 to control Rhizoctonia solani in rice (Oryza sativa)
AbstractThe incorporation of biological control agents (BCAs) such asTrichoderma spp. in agricultural systems favors the transition towards sustainable practices of plant nutrition and diseases control. Novel bioproducts for crop management are called to guarantee sustainable antagonism activity of BCAs and increase the acceptance of the farmers. The encapsulation in polymeric matrices play a prominent role for providing an effective carrier/protector and long-lasting bioproduct. This research aimed to study the influence of biopolymer in hydrogel capsules on survival and shelf-life ofT. koningiopsis. Thus, two hydrogel ca...
Source: World Journal of Microbiology and Biotechnology - February 26, 2024 Category: Microbiology Source Type: research

Synthesis of nanoparticles using Trichoderma Harzianum, characterization, antifungal activity and impact on Plant Growth promoting Bacteria
This study suggests that the synthesized nanoparticles have the potential to serve as antifungal compounds at special concentration against plant diseases without significantly reducing the potential of PGPB at low concentrations. (Source: World Journal of Microbiology and Biotechnology)
Source: World Journal of Microbiology and Biotechnology - February 24, 2024 Category: Microbiology Source Type: research

Shifting the pH profiles of Staphylococcus epidermidis lipase (SEL) and Staphylococcus hyicus lipase (SHL) through generating chimeric lipases by DNA shuffling strategy
AbstractEnzymes are often required to function in a particular reaction condition by the industrial procedure. In order to identify critical residues affecting the optimum pH ofStaphylococcal lipases, chimeric lipases from homologous lipases were generated via a DNA shuffling strategy. Chimeric 1 included mutations of G166S, K212E, T243A, H271Y. Chimeric 2 consisted of substitutions of K212E, T243A, H271Y. Chimeric 3 contained substitutions of K212E, R359L. From the screening results, the pH profiles for chimeric 1 and 2 lipases were shifted from pH 7 to 6. While the pH of chimeric 3 was shifted to 8. It seems the mutation...
Source: World Journal of Microbiology and Biotechnology - February 22, 2024 Category: Microbiology Source Type: research

Insights into the complementation potential of the extreme acidophile ’s orthologue in replacing Escherichia coli hfq gene—particularly in bacterial resistance to environmental stress
In this study, the recombinant strain Δhfq/Achfq, constructed by CRISPR/Cas9-mediated chromosome integration, fully or partially restored the phenotypic defects caused byhfq deletion inEscherichia coli, including impaired growth performance, abnormal cell morphology, impaired swarming motility, decreased stress resistance, decreased intracellular ATP and free amino acid levels, and attenuated biofilm formation. Particularly noteworthy, the intracellular ATP level and biofilm production of the recombinant strain were increased by 12.2% and 7.0%, respectively, compared to the Δhfq mutant. Transcriptomic analysis revealed t...
Source: World Journal of Microbiology and Biotechnology - February 22, 2024 Category: Microbiology Source Type: research

Tiny but mighty: metal nanoparticles as effective antimicrobial agents for plant pathogen control
AbstractMetal nanoparticles (MNPs) have gained significant attention in recent years for their potential use as effective antimicrobial agents for controlling plant pathogens. This review article summarizes the recent advances in the role of MNPs in the control of plant pathogens, focusing on their mechanisms of action, applications, and limitations. MNPs can act as a broad-spectrum antimicrobial agent against various plant pathogens, including bacteria, fungi, and viruses. Different types of MNPs, such as silver, copper, zinc, iron, and gold, have been studied for their antimicrobial properties. The unique physicochemical...
Source: World Journal of Microbiology and Biotechnology - February 19, 2024 Category: Microbiology Source Type: research

Biodesulfurization of organosulfur compounds by a trehalose biosurfactant producing Gordonia sp. isolated from crude oil contaminated soil
AbstractCertain factors hinder the commercialization of biodesulfurization process, including low substrate-specificity of the currently reported desulfurizing bacteria and restricted mass transfer of organic-sulfur compounds in biphasic systems. These obstacles must be addressed to clean organic-sulfur rich petro-fuels that pose serious environmental and health challenges. In current study, a dibenzothiophene desulfurizing strain,Gordonia rubripertincta W3S5 (source: oil contaminated soil) was systematically evaluated for its potential to remove sulfur from individual compounds and mixture of organic-sulfur compounds. Met...
Source: World Journal of Microbiology and Biotechnology - February 19, 2024 Category: Microbiology Source Type: research

Methods for the separation of hydraulic retention time and solids retention time in the application of photosynthetic microorganisms in photobioreactors: a review
AbstractPhotosynthetic microorganisms have a wide range of biotechnical applications, through the application of their versatile metabolisms. However, their use in industry has been extremely limited to date, partially because of the additional complexities associated with their cultivation in comparison to other organisms. Strategies and developments in photobioreactors (PBRs) designed for their culture and applications are needed to drive the field forward. One particular area which bears examination is the use of strategies to separate solid- and hydraulic-residence times (SRT and HRT), to facilitate flow-through system...
Source: World Journal of Microbiology and Biotechnology - February 17, 2024 Category: Microbiology Source Type: research

Revisiting the smart metallic nanomaterials: advances in nanotechnology-based antimicrobials
AbstractDespite significant advancements in diagnostics and treatments over the years, the problem of antimicrobial drug resistance remains a pressing issue in public health. The reduced effectiveness of existing antimicrobial drugs has prompted efforts to seek alternative treatments for microbial pathogens or develop new drug candidates. Interestingly, nanomaterials are currently gaining global attention as a possible next-generation antibiotics. Nanotechnology holds significant importance, particularly when addressing infections caused by multi-drug-resistant organisms. Alternatively, these biomaterials can also be combi...
Source: World Journal of Microbiology and Biotechnology - February 17, 2024 Category: Microbiology Source Type: research