First description of adenosine production by Gnomoniopsis smithogilvyi, causal agent of chestnut brown rot
AbstractGnomoniopsis smithogilvyi (Gnomoniaceae,Diaporthales) is the main causal agent of chestnut brown rot on sweet chestnut worldwide. The rotting of nuts leads to alterations in the organoleptic qualities and decreased fruit production, resulting in significant economic losses. In 2021, there was an important outbreak of chestnut rot in southern Galicia (Spanish northwest). The profile of secondary metabolites fromG. smithogilvyi was studied, especially to determine its capability for producing mycotoxins, as happens with other rotting fungi, due to the possible consequences on the safety of chestnut consumption. Secon...
Source: World Journal of Microbiology and Biotechnology - March 28, 2024 Category: Microbiology Source Type: research

Differentiation of closely-related species within Acinetobacter baumannii-calcoaceticus complex via Raman spectroscopy: a comparative machine learning analysis
In this study, we combined surfaced-enhanced Raman spectroscopy (SERS) with a set of machine learning algorithms for identifying species within the Acb complex. According to the results, the support vector machine (SVM) model achieved the best prediction accuracy at 98.33% with a fivefold cross-validation rate of 96.73%. Taken together, this study confirms that the SERS-SVM method provides a convenient way to discriminate betweenA. baumannii,Acinetobacter pittii, andAcinetobacter nosocomialis in the Acb complex, which shows an application potential for species identification ofAcinetobacter baumannii-calcoaceticus complex ...
Source: World Journal of Microbiology and Biotechnology - March 28, 2024 Category: Microbiology Source Type: research

Improvement of violacein production using abiotic stresses and microbial adaptation
AbstractThe aim of the current research was to improve violacein production withJanthinobacterium lividum using abiotic stresses and bacterial adaptation against stress. Initially, the effect of carbon sources and the medium volume: air ratio on violacein production was assessed. Then, the production of violacein under hydrogen peroxide (H2O2) and ampicillin (Amp) stresses and acyl homoserine lactone (AHL) was evaluated. In the next step,J. lividum was adapted against increased concentrations of Amp. Finally, the production of violacein was analyzed in adapted bacterium cultivated in the presence of optimal amounts of H2O2...
Source: World Journal of Microbiology and Biotechnology - March 28, 2024 Category: Microbiology Source Type: research

Selenium enriched bifidobacteria and lactobacilli as potential dietary supplements
In this study, we tested the ability of lactobacilli and bifidobacteria strains to accumulate and biotransform sodium selenite into various selenium species, including selenium nanoparticles (SeNPs). Selenium tolerance and cytotoxicity of selenized strains towards human adenocarcinoma Caco-2 and HT29 cells were determined for all tested strains. Furthermore, the influence of selenium enrichment on the antioxidant activity of selenized strains and hydrophobicity of the bacterial cell surfaces were evaluated. Both hydrophobicity and antioxidant activity increased significantly in the selenizedL. paracasei strain and decrease...
Source: World Journal of Microbiology and Biotechnology - March 27, 2024 Category: Microbiology Source Type: research

Advances in recombinant protease production: current state and perspectives
AbstractProteases, enzymes that catalyze the hydrolysis of peptide bonds in proteins, are important in the food industry, biotechnology, and medical fields. With increasing demand for proteases, there is a growing emphasis on enhancing their expression and production through microbial systems. However, proteases ’ native hosts often fall short in high-level expression and compatibility with downstream applications. As a result, the recombinant production of proteases has become a significant focus, offering a solution to these challenges. This review presents an overview of the current state of protease p roduction in pr...
Source: World Journal of Microbiology and Biotechnology - March 27, 2024 Category: Microbiology Source Type: research

Progress in polystyrene biodegradation by insect gut microbiota
AbstractPolystyrene (PS) is frequently used in the plastics industry. However, its structural stability and difficulty to break down lead to an abundance of plastic waste in the environment, resulting in micro-nano plastics (MNPs). As MNPs are severe hazards to both human and environmental health, it is crucial to develop innovative treatment technologies to degrade plastic waste. The biodegradation of plastics by insect gut microorganisms has gained attention as it is environmentally friendly, efficient, and safe. However, our knowledge of the biodegradation of PS is still limited. This review summarizes recent research a...
Source: World Journal of Microbiology and Biotechnology - March 26, 2024 Category: Microbiology Source Type: research

Hypothetical protein MAA_07646 is required for stress resistance and pathogenicity in Metarhizium robertsii
In conclusion,MAA_07646 contributes to thermotolerance, UV resistance, and virulence inM. robertsii. Understanding its function sheds light on the insecticidal potential ofM. robertsii’s hypothetical proteins. (Source: World Journal of Microbiology and Biotechnology)
Source: World Journal of Microbiology and Biotechnology - March 23, 2024 Category: Microbiology Source Type: research

Oxygen mediated mobilization and co-occurrence of antibiotic resistance in lab-scale bioreactor using metagenomic binning
AbstractSub-lethal levels of antibiotic stimulate bacteria to generate reactive oxygen species (ROS) that promotes emergence and spread of antibiotic resistance mediated by mobile genetic elements (MGEs). Nevertheless, the influence of dissolved oxygen (DO) levels on mobility of antibiotic resistance genes (ARGs) in response to ROS-induced stress remains elusive. Thus, the study employs metagenomic assembly and binning approaches to decipher mobility potential and co-occurrence frequency of ARGs and MGEs under hyperoxic (5.5-7 mgL− 1), normoxic (2.5-4 mgL− 1), and hypoxic (0.5-1 mgL− 1) conditions in lab-scale ...
Source: World Journal of Microbiology and Biotechnology - March 23, 2024 Category: Microbiology Source Type: research

Adsorption of azo dye by biomass and immobilized Yarrowia lipolytica; equilibrium, kinetic and thermodynamic studies
AbstractOne of the major environmental problems we have today is dye pollution, primarily caused by the textile industry. This pollution has detrimental effects on aquatic life, soil fertility, and human health. Many microbial biosorbents have been documented in the literature for the removal of a wide range of azo dyes commonly employed in the textile industry. However,Yarrowia lipolytica NBRC1658 is firstly used as both free and immobilized sorbents for the removal of Reactive yellow 18 (RY18), acid red 18 (AR18) and basic blue 41 (BB41) in this study. The effect of experimental conditions such as pH, biosorbent quantity...
Source: World Journal of Microbiology and Biotechnology - March 22, 2024 Category: Microbiology Source Type: research

The anticancer effect of potential probiotic L. fermentum and L. plantarum in combination with 5-fluorouracil on colorectal cancer cells
In conclusion, the cell extracts of the lactobacilli strains probably can act as a potential complementary anticancer therapy. (Source: World Journal of Microbiology and Biotechnology)
Source: World Journal of Microbiology and Biotechnology - March 22, 2024 Category: Microbiology Source Type: research

Efficiency of thermostable purified laccase isolated from Physisporinus vitreus for azo dyes decolorization
AbstractLaccases are versatile biocatalysts that are prominent for industrial purposes due to their extensive substrate specificity. Therefore, this research investigated producing laccase fromPhysisporinus vitreus via liquid fermentation. The results revealed that veratryl alcohol (4mM) was the most effective inducer 7500U/L. On the other hand, Zn ions inhibited laccase production. The optimum carbon and nitrogen sources were glucose and tryptone by 5200 and 3300 U/L, respectively. Moreover, solvents exhibited various impacts on the enzyme activity at three different solvent concentrations (5%, 10% and 20%), however, it s...
Source: World Journal of Microbiology and Biotechnology - March 21, 2024 Category: Microbiology Source Type: research

Performance evaluation of Trichoderma reseei in tolerance and biodegradation of diuron herbicide in agar plate, liquid culture and solid-state fermentation
AbstractThe present study evaluated the performance of the fungusTrichoderma reesei to tolerate and biodegrade the herbicide diuron in its agrochemical presentation in agar plates, liquid culture, and solid-state fermentation. The tolerance ofT. reesei to diuron was characterized through a non-competitive inhibition model of the fungal radial growth on the PDA agar plate and growth in liquid culture with glucose and ammonium nitrate, showing a higher tolerance to diuron on the PDA agar plate (inhibition constant 98.63 mg L−1) than in liquid culture (inhibition constant 39.4 mg L−1). Diuron biodegradation byT. reesei wa...
Source: World Journal of Microbiology and Biotechnology - March 20, 2024 Category: Microbiology Source Type: research

Rice N-biofertilization by inoculation with an engineered photosynthetic diazotroph
AbstractPhotosynthetic diazotrophs expressing iron-only (Fe-only) nitrogenase can be developed into a promising biofertilizer, as it is independent on the molybdenum availability in the soil. However, the expression of Fe-only nitrogenase in diazotrophs is repressed by the fixed nitrogen of the soil, limiting the efficiency of nitrogen fixation in farmland with low ammonium concentrations that are inadequate for sustainable crop growth. Here, we succeeded in constitutively expressing the Fe-only nitrogenase even in the presence of ammonium by controlling the transcription of Fe-only nitrogenase gene cluster (anfHDGK) with ...
Source: World Journal of Microbiology and Biotechnology - March 19, 2024 Category: Microbiology Source Type: research

Microbial lipopeptides: their pharmaceutical and biotechnological potential, applications, and way forward
AbstractAs lead molecules, cyclic lipopeptides with antibacterial, antifungal, and antiviral properties have garnered a lot of attention in recent years. Because of their potential, cyclic lipopeptides have earned recognition as a significant class of antimicrobial compounds with applications in pharmacology and biotechnology. These lipopeptides, often with biosurfactant properties, are amphiphilic, consisting of a hydrophilic moiety, like a carboxyl group, peptide backbone, or carbohydrates, and a hydrophobic moiety, mostly a fatty acid. Besides, several lipopeptides also have cationic groups that play an important role i...
Source: World Journal of Microbiology and Biotechnology - March 15, 2024 Category: Microbiology Source Type: research

In-vitro selection of lactic acid bacteria to combat Salmonella enterica and Campylobacter jejuni in broiler chickens
In conclusion,Leuconostoc mesenteroides displayed the most significant overall anti-pathogenic effects when compared to the other LAB strains studied, indicating its potential applicationin-vivo. (Source: World Journal of Microbiology and Biotechnology)
Source: World Journal of Microbiology and Biotechnology - March 14, 2024 Category: Microbiology Source Type: research