World Journal of Microbiology and Biotechnology 
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This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.Superwettable surfaces and factors impacting microbial adherence in microbiologically-influenced corrosion: a review
AbstractMicrobiologically-influenced corrosion (MIC) is a common operational hazard to many industrial processes. The focus of this review lies on microbial corrosion in the maritime industry. Microbial metal attachment and colonization are the critical steps in MIC initiation. We have outlined the crucial factors influencing corrosion caused by microorganism sulfate-reducing bacteria (SRB), where its adherence on the metal surface leads to Direct Electron Transfer (DET) —MIC. This review thus aims to summarize the recent progress and the lacunae in mitigation of MIC. We further highlight the susceptibility of stainless ...
Source: World Journal of Microbiology and Biotechnology - February 14, 2024 Category: Microbiology Source Type: research
Thread-powered cell lysis and isotachophoresis: unlocking microbial DNA for diverse molecular applications
AbstractCentral to the domain of molecular biology resides the foundational process of DNA extraction and purification, a cornerstone underpinning a myriad of pivotal applications. In this research, we introduce a DNA extraction and purification technique leveraging polypropylene (PP) threads. The process commences with robust cell lysis achieved through the vigorous agitation of interwoven PP threads. The friction between the threads facilitates cell lysis especially those microbes having tough cell wall. For purification of DNA, thread-based isotachophoresis was employed which makes the whole process swift and cost-effec...
Source: World Journal of Microbiology and Biotechnology - February 13, 2024 Category: Microbiology Source Type: research
Metagenomic analysis of carbohydrate-active enzymes and their contribution to marine sediment biodiversity
In this study, we employ a metagenomic methodology to investigate the distribution and abundance of carbohydrate-active enzymes (CAZymes) in 37 marine sediments sites. These sediments exhibit varying oxygen availability and were isola ted in diverse regions worldwide. Our comparative analysis is based on the metabolic potential for oxygen utilisation, derived from genes present in both oxic and anoxic environments. We found that extracellular CAZyme modules targeting the degradation of plant and algal detritus, necromass, and hos t glycans were abundant across all metagenomic samples. The analysis of these results indicate...
Source: World Journal of Microbiology and Biotechnology - February 13, 2024 Category: Microbiology Source Type: research
Strategies for the biological synthesis of D-glucuronic acid and its derivatives
AbstractD-glucuronic acid is a kind of glucose derivative, which has excellent properties such as anti-oxidation, treatment of liver disease and hyperlipidemia, and has been widely used in medicine, cosmetics, food and other fields. The traditional production methods of D-glucuronic acid mainly include natural extraction and chemical synthesis, which can no longer meet the growing market demand. The production of D-glucuronic acid by biocatalysis has become a promising alternative method because of its high efficiency and environmental friendliness. This review describes different production methods of D-glucuronic acid, i...
Source: World Journal of Microbiology and Biotechnology - February 13, 2024 Category: Microbiology Source Type: research
Multilocus genotyping for classification and genetic structuring of Lactobacillus casei: insights from source and geographical origin
AbstractThe aim of the study in this article is to systematise the newly introduced strains ofLactobacillus based on determining the nucleotide sequence of a particular set of their genes (loci). The primary approach employed to address this issue involves conducting a laboratory experiment. During this experiment, a thorough examination was carried out on a set of organic compounds consisting of small DNA elements from theLactobacillus genus. TheMultilocus genotyping method served as the central technique, complemented by additional molecular-biological and population methods. These additional methods were utilized to det...
Source: World Journal of Microbiology and Biotechnology - February 13, 2024 Category: Microbiology Source Type: research
Evaluation of stability of (1R,2 S)-(−)-2-methylamino-1-phenyl-1-propanol hydrochloride in plasma and urine samples-inoculated with Escherichia coli using high-performance liquid chromatography (HPLC)
AbstractThe preservation of drug stability in biological evidence during the processes of collection and storage poses a substantial obstacle to the progress of forensic investigations. In conjunction with other constituents, the microorganisms present in the samples play a vital role in this investigation. The present investigation employed the high-performance liquid chromatography (HPLC) technique to assess the stability of (1R,2 S)-(–)-2-methylamino-1-phenyl-1-propanol hydrochloride in plasma and urine samples that were inoculated withEscherichia coli. These samples were subjected to storage conditions of 37 °C ...
Source: World Journal of Microbiology and Biotechnology - February 13, 2024 Category: Microbiology Source Type: research
Evaluation of the efficiency of thermostable l-asparaginase from B. licheniformis UDS-5 for acrylamide mitigation during preparation of French fries
AbstractA thermostablel-asparaginase was produced fromBacillus licheniformis UDS-5 (GenBank accession number, OP117154). The production conditions were optimized by the Plackett Burman method, followed by the Box Behnken method, where the enzyme production was enhanced up to fourfold. It secretedl-asparaginase optimally in the medium, pH 7, containing 0.5% (w/v) peptone, 1% (w/v) sodium chloride, 0.15% (w/v) beef extract, 0.15% (w/v) yeast extract, 3% (w/v)l-asparagine at 50 °C for 96 h. The enzyme, with a molecular weight of 85 kDa, was purified by ion exchange chromatography and size exclusion chromatography with bett...
Source: World Journal of Microbiology and Biotechnology - February 12, 2024 Category: Microbiology Source Type: research
Enhancement of α-galactosidase production using novel Actinoplanes utahensis B1 strain: sequential optimization and purification of enzyme
This study demonstrate s the isolation ofActinoplanes utahensis B1 and optimization of the process for the α-galactosidase production as well as single-step purification.Graphical abstract (Source: World Journal of Microbiology and Biotechnology)
Source: World Journal of Microbiology and Biotechnology - February 12, 2024 Category: Microbiology Source Type: research
Harnessing the potential of bimetallic nanoparticles: Exploring a novel approach to address antimicrobial resistance
In this study, the promising potential of bimetallic nanoparticles (BMNPs) was investigated as a novel weapon against AMR. This research begins by elaborating on the gravity of the AMR problem, outlining its scope in terms of the effects on healthcare systems, and stressing the urgent need for novel solutions. Because of their unusual features and wide range of potential uses, bimetallic nanoparticles (BMNPs), which are tiny particles consisting of two different metal elements, have attracted a lot of interest in numerous fields. This review article provides a comprehensive analysis of the composition, structural character...
Source: World Journal of Microbiology and Biotechnology - February 10, 2024 Category: Microbiology Source Type: research
A biomedical perspective of pyocyanin from Pseudomonas aeruginosa: its applications and challenges
AbstractPyocyanin is a bioactive pigment produced byPseudomonas aeruginosa. It is an important virulence factor that plays a critical role inP. aeruginosa infections as a redox-active secondary metabolite and a quorum sensing (QS) signaling molecule. Pyocyanin production from chorismic acid requires the involvement of two homologous operons,phz1 andphz2, which are activated by QS regulatory proteins. Pyocyanin inhibits the proliferation of bacterial, fungal, and mammalian cells by inducing oxidative stress due to which it acts as a potent antibacterial, antifungal, and anticancer agent. Its potential role as a neuroprotect...
Source: World Journal of Microbiology and Biotechnology - February 10, 2024 Category: Microbiology Source Type: research
Isolation of local strains of the yeast Metschnikowia for biocontrol and lipid production purposes
AbstractThe bioprospection of indigenous microorganism strains with biotechnological potential represents a prominent trend.Metschnikowia yeasts exhibit diverse capabilities, such as ethanol reduction in winemaking, biocontrol potential, and lipid production. In this work, localMetschnikowia strains were isolated from different fruits by their ability to produce pulcherrimic acid, a molecule that has been linked to biocontrol activity and that binds iron giving colored colonies. Five strains were selected, each from one of five distinct sources. All of them were identified asM. pulcherrima. All five were able inhibit other...
Source: World Journal of Microbiology and Biotechnology - February 9, 2024 Category: Microbiology Source Type: research
Two at once: simultaneous increased production of astaxanthin and mycosporines in a single batch culture using a Phaffia rhodozyma mutant strain
AbstractPhaffia rhodozyma is a basidiomycetous yeast characterized by its production of the carotenoid pigment astaxanthin, which holds high commercial value for its significance in aquaculture, cosmetics and as nutraceutics, and the UV-B-absorbing compound mycosporine-glutaminol-glucoside (MGG), which is of great biotechnological relevance for its incorporation into natural sunscreens. However, the industrial exploitation has been limited to the production of astaxanthin in small quantities. On the other hand, the accumulation of MGG inP. rhodozyma was recently reported and could add value to the simultaneous production o...
Source: World Journal of Microbiology and Biotechnology - February 8, 2024 Category: Microbiology Source Type: research
Antimicrobial and antibiofilm properties of selenium-chitosan-loaded salicylic acid nanoparticles for the removal of emerging contaminants from bacterial pathogens
In this study salicylic acid loaded containing selenium nanoparticles was synthesized and called SA@CS-Se NPs. the chitosan was used as a natural stabilizer during the synthesis process. Fourier transforms infrared spectroscopy (FTIR), Powder X-ray diffraction (XRD), field emission electron microscopy (FESEM), and transmission electron microscopy (TEM) were used to describe the physicochemical characteristics of the SA@CS-Se NPs. The PXRD examination revealed that the grain size was around 31.9 nm. TEM and FESEM techniques showed the spherical shape of SA@CS-Se NPs. Additionally, the analysis of experiments showed that S...
Source: World Journal of Microbiology and Biotechnology - February 6, 2024 Category: Microbiology Source Type: research
Application of sequential design for enhanced l-asparaginase synthesis from Ganoderma australe GPC191
AbstractWith an increasing demand forl-asparaginase in pharmaceutical and food sectors for its cytostatic and acrylamide-reducing qualities, there's a need to discover novel, highly productive enzyme sources with improved pharmacokinetic profiles. Keeping this in mind, the present study aimed at maximizing the potential ofGanoderma australe GPC191 to producel-asparaginase by fermentation medium optimization using statistical validation. Of the 11 physicochemical parameters evaluated under submerged fermentation conditions through one-factor-at-a-time approach and Plackett –Burman design, only four parameters (inoculum lo...
Source: World Journal of Microbiology and Biotechnology - February 1, 2024 Category: Microbiology Source Type: research
Microbial α-L-arabinofuranosidases: diversity, properties, and biotechnological applications
AbstractArabinoxylans (AXs) are hemicellulosic polysaccharides consisting of a linear backbone ofβ-1,4-linked xylose residues branched by high content ofα-L-arabinofuranosyl (Araf) residues along with other side-chain substituents, and are abundantly found in various agricultural crops especially cereals. The efficient bioconversion of AXs into monosaccharides, oligosaccharides and/or other chemicals depends on the synergism of main-chain enzymes and de-branching enzymes. Exo-α-L-arabinofuranosidases (ABFs) catalyze the hydrolysis of terminal non-reducingα-1,2-,α-1,3- orα-1,5- linkedα-L-Araf residues from arabinose-...
Source: World Journal of Microbiology and Biotechnology - January 31, 2024 Category: Microbiology Source Type: research
