World Journal of Microbiology and Biotechnology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Prevalence and distribution pattern of AmpC β-lactamases in ESBL producing clinical isolates of Klebsiella spp. in parts of Assam, India
AbstractThe production of extended-spectrum β-lactamases (ESBLs) and AmpC β-lactamases is the most common explanation of multidrug resistance in clinical isolates ofKlebsiella spp. In the present study, a total of 160 isolates ofKlebsiella spp. were procured from the DBT-NER project with ethical clearance no. DU/Dib/ECBHR(Human)/2021-22/02). These were collected from various health settings of Assam and identified as drug-resistant. The isolates were screened for antibiotic susceptibility and phenotypic tests were performed on multidrug resistant isolates to confirm ESBL and AmpC β-lactamases production. The distributio...
Source: World Journal of Microbiology and Biotechnology - December 8, 2023 Category: Microbiology Source Type: research

The flexible linker and CotG were more effective for the spore surface display of keratinase KERQ7
AbstractFeather, horn, hoof, and other keratin waste are protein-rich but limited by natural keratinase synthesis, activity, pH, and temperature stability. It is challenging to realize its large-scale application in industries.Bacillus subtilis spores are a safe, efficient, and highly resistant immobilized carrier, which can improve target proteins ’ resistance. In this research,KERQ7, the keratinase gene ofBacillus tequilensis strain Q7, was fused to theBacillus subtilis genes coding for the coat proteins CotG and CotB, respectively, and displayed on the surface ofB. subtilis spores. Compared with the free KERQ7, the im...
Source: World Journal of Microbiology and Biotechnology - December 7, 2023 Category: Microbiology Source Type: research

Endophytic fungi of Lycium barbarum: isolation, determination, bioactivity and separation of compounds
In conclusion, endophytic fungi ofL. barbarum from eastern China can serve as a potential source of active natural products with antibacterial and antioxidant properties. (Source: World Journal of Microbiology and Biotechnology)
Source: World Journal of Microbiology and Biotechnology - December 7, 2023 Category: Microbiology Source Type: research

Cytochrome P450 for environmental remediation:  catalytic mechanism, engineering strategies and future prospects
This article presents an overview of the current understanding of various members of the CYP450 superfamily involved in transforming organic pollutants and the engineering of biodegrading CYP450s. Additionally, it explores the catalytic mechanisms, current practical applications of CYP450-based systems, their potential applications, and the prospects in bioremediation. (Source: World Journal of Microbiology and Biotechnology)
Source: World Journal of Microbiology and Biotechnology - December 7, 2023 Category: Microbiology Source Type: research

Characterization of a novel sucrose phosphorylase from Paenibacillus elgii and its use in biosynthesis of α-arbutin
In this study, a previously reported SPase known for its effectiveness in synthesizing α-arbutin, was u sed as a probe sequence to identify a novel SPase fromPaenibacillus elgii (PeSP) in the protein database. The sequence similarity betweenPeSP and the probe was 39.71%, indicating a degree of novelty. Subsequently, the gene encodingPeSP was coexpressed with the molecular chaperone pG-Tf2 inEscherichia coli, significantly improvingPeSP ’s solubility. Following this,PeSP was characterized and employed for α-arbutin biosynthesis. The specific activity of co-expressedPeSP reached 169.72 U/mg, exhibited optimal activity at...
Source: World Journal of Microbiology and Biotechnology - December 7, 2023 Category: Microbiology Source Type: research

Whole genome sequencing reveals candidate genes involving in PAS resistance in M. Tuberculosis isolated from patients in Thailand
AbstractPara-amino salicylic acid (PAS) was first reported by Lehmann in 1946 and used for tuberculosis treatment. However, due to its adverse effects, it is now used only as a second line anti-tuberculosis drug for treatment of multidrug resistant or extensively drug resistantM. tuberculosis. The structure of PAS is similar to para-amino benzoic acid (pABA), an intermediate metabolite in the folate synthesis pathway. The study has identified mutations in genes in folate pathway and their intergenic regions for their possibilities in responsible for PAS resistance. Genomic DNA from 120 PAS-resistant and 49 PAS-sensitiveM. ...
Source: World Journal of Microbiology and Biotechnology - December 7, 2023 Category: Microbiology Source Type: research

Analysis of rhizosphere fungal community of agricultural crops cultivated in laboratory experiments on Chernevaya taiga soil
AbstractChernevaya taiga of Western Siberia, Russia, is a unique ecosystem characterized by fertile soil, exceptionally large herbaceous plant sizes, and extraordinarily rapid rates of plant residue degradation. We expected that growing crops on soil collected from Chernevaya taiga, which has never been used for agricultural purposes before, would result in a distinct rhizospheric fungal community. This community could potentially yield novel, potent biostimulators and biocontrol fungi for modern agriculture. To check this idea, we used high-throughput ITS sequencing to examine the microbial communities in the rhizosphere ...
Source: World Journal of Microbiology and Biotechnology - December 7, 2023 Category: Microbiology Source Type: research

Biochemical and microbiological characterization of a thermotolerant bacterial consortium involved in the corrosion of Aluminum Alloy 7075
AbstractMicroorganisms can play a significant role in material corrosion, with bacterial biofilms as major participants in microbially influenced corrosion (MIC). The exact mechanisms by which this takes place are poorly understood, resulting in a scarcity of information regarding MIC detection and prevention. In this work, a consortium of moderately thermophilic bacteria isolated from a biofilm growing over aluminum alloy 7075 was characterized. Its effect over the alloy was evaluated on a 40-day period using Electron Microscopy, demonstrating acceleration of corrosion in comparison to the abiotic control. The bacterial c...
Source: World Journal of Microbiology and Biotechnology - December 7, 2023 Category: Microbiology Source Type: research

Salinity-controlled distribution of prokaryotic communities in the Arctic sea-ice melt ponds
AbstractThe thawing of snow and sea ice produces distinctive melt ponds on the surface of the Arctic sea ice, which covers a significant portion of the surface sea ice during summer. Melt-pond salinity impacts heat transfer to the ice below and the melting rate. It is widely known that melt ponds play a significant role in heat fluxes, ice-albedo feedback, and sea-ice energy balance. However, not much attention has been given to the fact that melt ponds also serve as a unique microbial ecosystem where microbial production begins as soon as they are formed. Here, we investigated the role of melt pond salinity in controlling...
Source: World Journal of Microbiology and Biotechnology - December 7, 2023 Category: Microbiology Source Type: research

Biodegradation of furfuryl alcohol by indigenous Bacillus species of industrial effluent-contaminated sites: estimation, biokinetics and toxicity assessment of bio-transformed metabolites
AbstractFurfuryl alcohol (FA) and other furanic compounds have garnered considerable attention in the quest for sustainable alternatives. FA-based resins have been used in various sectors that entail the release of FA into the environment. Hence, to ensure sustainability in this scenario, devising a dependable approach to its degradation is imperative. Given the crucial role of bacterial strains in the biodegradation of various organic pollutants, this study investigates the microbial degradation of FA, using bacterial strains isolated from sites that are constantly exposed to industrial waste. Three potential isolates wer...
Source: World Journal of Microbiology and Biotechnology - December 7, 2023 Category: Microbiology Source Type: research

Identification and characterization of a versatile keratinase, KerZJ, from Stenotrophomonas sp. LMY
AbstractKeratinases have drawn increasing attention in recent decades owing to their catalytic versatility and broad applications from agriculture to medicine. In the present study, we isolated a highly keratinolytic and fibrinolytic bacterium from the campus soil and named itStenotrophomonas sp. LMY based on genetic information. To identify the potential keratinase genes, the genome sequence of the strain was obtained and analyzed. Sequence alignment and comparison revealed that the protein 1_737 (KerZJ) had the highest sequence homology to a reported keratinase KerBL. We recombinantly expressed KerZJ inEscherichia coli O...
Source: World Journal of Microbiology and Biotechnology - December 7, 2023 Category: Microbiology Source Type: research

Antifungal properties of cathelicidin LL-37: current knowledge and future research directions
AbstractThe threat of fungal diseases is substantially underestimated worldwide, but they have serious consequences for humans, animals, and plants. Given the limited number of existing antifungal drugs together with the emergence of drug-resistant strains, many researchers have actively sought alternatives or adjuvants to antimycotics. The best way to tackle these issues is to unearth potential antifungal agents with new modes of action. Antimicrobial peptides are being hailed as a promising source of novel antimicrobials since they exhibit rapid and broad-spectrum microbicidal activities with a reduced likelihood of deve...
Source: World Journal of Microbiology and Biotechnology - December 7, 2023 Category: Microbiology Source Type: research

Microbial synthesis of pyrroloquinoline quinone
AbstractPyrroloquinoline quinone (PQQ) is a peptide-modified natural product. PQQ has important physiological functions such as anti-oxidation, anti-aging, and immunity enhancement. However, due to the lack of in-depth understanding of PQQ biosynthesis and regulation, inefficient PQQ production level limits its wide application. Accordingly, there is still an urgent need to develop high-yielding strains for synthesis of PQQ. This paper reviewed the research and development trends on the PQQ biosynthetic pathways, catalytic reaction mechanism of key enzymes, and the selection of high-yielding strains, which also prospects f...
Source: World Journal of Microbiology and Biotechnology - December 7, 2023 Category: Microbiology Source Type: research

Towards industrial biological hydrogen production: a review
AbstractIncreased production of renewable energy sources is becoming increasingly needed. Amidst other strategies, one promising technology that could help achieve this goal is biological hydrogen production. This technology uses micro-organisms to convert organic matter into hydrogen gas, a clean and versatile fuel that can be used in a wide range of applications. While biohydrogen production is in its early stages, several challenges must be addressed for biological hydrogen production to become a viable commercial solution. From an experimental perspective, the need to improve the efficiency of hydrogen production, the ...
Source: World Journal of Microbiology and Biotechnology - December 7, 2023 Category: Microbiology Source Type: research

From microbes to molecules: a review of microbial-driven antioxidant peptide generation
AbstractOxidative stress, arising from excess reactive oxygen species (ROS) or insufficient antioxidant defenses, can damage cellular components, such as lipids, proteins, and nucleic acids, resulting in cellular dysfunction. The relationship between oxidative stress and various health disorders has prompted investigations into potent antioxidants that counteract ROS ’s detrimental impacts. In this context, antioxidant peptides, composed of two to twenty amino acids, have emerged as a unique group of antioxidants and have found applications in food, nutraceuticals, and pharmaceuticals. Antioxidant peptides are sourced fr...
Source: World Journal of Microbiology and Biotechnology - December 7, 2023 Category: Microbiology Source Type: research