Application of sequential design for enhanced l-asparaginase synthesis from Ganoderma australe GPC191

AbstractWith an increasing demand forl-asparaginase in pharmaceutical and food sectors for its cytostatic and acrylamide-reducing qualities, there's a need to discover novel, highly productive enzyme sources with improved pharmacokinetic profiles. Keeping this in mind, the present study aimed at maximizing the potential ofGanoderma australe GPC191 to producel-asparaginase by fermentation medium optimization using statistical validation. Of the 11 physicochemical parameters evaluated under submerged fermentation conditions through one-factor-at-a-time approach and Plackett –Burman design, only four parameters (inoculum load,l-asparagine, soybean meal, and initial pH) influencedl-asparaginase production, significantly (p <  0.001). The optimal levels and interaction effects of these on the overall production were further evaluated by the central composite rotatable design of response surface methodology. Post-optimization, 27.34 U/mL was predicted as the maximum activity at pH 7 with 5n inoculum load and 15 g/L eac h ofl-asparagine and soybean meal. Experimental validation yielded an activity of 28.52 U/mL, indicating an overall 18.17-fold increase from the unoptimized stage. To our knowledge, this is the first report signifying thel-asparaginase production aptitude ofG. australe with sequential statistical validation using agricultural waste, which can serve as a model to enhance its yields, offering a sustainable and cost-effective solution for industrial applicatio...
Source: World Journal of Microbiology and Biotechnology - Category: Microbiology Source Type: research