Phylogenetic characterization of Orthobunyaviruses isolated from Trinidad shows evidence of natural reassortment
This study also highlights the importance of complementing serological identification with nucleotide sequencing when characterizing orthobunyaviruses. (Source: Virus Genes)
Source: Virus Genes - February 10, 2023 Category: Genetics & Stem Cells Source Type: research
The global asymptotical stability of pseudorabies model with age-structure
This study establishes a solid theoretical framework and a rigorous mathematical formulation for the prevention and control of pseudorabies. (Source: Virus Genes)
Source: Virus Genes - February 9, 2023 Category: Genetics & Stem Cells Source Type: research
Quantitative analysis of Epstein –Barr virus DNA in plasma and stomach biopsies of patients with gastric cancer
AbstractEpstein –Barr virus (EBV) associated gastric carcinoma (EBVaGC) is a subtype of gastric cancer with distinct histological and molecular features. The study aimed to assess the EBV DNA copy number and the prevalence of EBVaGC in gastric cancer samples taken from Iranian patients. The next aim was to assess whether the DNA and microRNAs EBV are present in plasma. EBV load was analyzed in 68 gastric cancer biopsies and compared with the results of EBV-encoded small RNA in situ hybridization (EBER-ISH) test in these patients. After the detection of 6 EBV miRNAs in gastric tissue by stem-loop RT-PCR, pla sma samples w...
Source: Virus Genes - February 9, 2023 Category: Genetics & Stem Cells Source Type: research
Correction: Whole genome sequencing and annotation of a lysogenic phage vB_EcoP_DE5 isolated from donkey-derived Escherichia coli
(Source: Virus Genes)
Source: Virus Genes - February 7, 2023 Category: Genetics & Stem Cells Source Type: research
Molecular detection of monkeypox and related viruses: challenges and opportunities
AbstractThe recent widespread emergence of monkeypox (mpox), a rare and endemic zoonotic disease by monkeypox virus (MPXV), has made global headlines. While transmissibility (R0≈ 0.58) and fatality rate (0–3%) are low, as it causes prolonged morbidity, the World Health Organization has declared monkeypox as a public health emergency of international concern. Thus, effective containment and disease management require quick and efficient detection of MPXV. In this bioinf ormatic overview, we summarize the numerous molecular tests available for MPXV, and discuss the diversity of genes and primers used in the polymerase ch...
Source: Virus Genes - February 6, 2023 Category: Genetics & Stem Cells Source Type: research
Diversity of viroids infecting grapevines in the South African Vitis germplasm collection
In this study, RNA sequence (RNA-Seq) data, from 229Vitis accessions from the field-maintained vineyard of the South AfricanVitis germplasm collection, were analysed to determine the diversity of the viroids present. Five of the seven known grapevine-infecting viroids and one putative grapevine-infecting viroid species were very commonly found, with 214 of the 229 samples containing at least one viroid species. HSVd, GYSVd-1, GYSVd-2, AGVd, and JGVd, as well as GHVd, were identified in the RNA-Seq data of the samples and confirmed using RT-PCR and Sanger sequencing. The HSVd sequences indicated the presence of two variants...
Source: Virus Genes - February 6, 2023 Category: Genetics & Stem Cells Source Type: research
Nuclear respiratory factor 1 promotes the progression of EBV-associated gastric cancer and maintains EBV latent infection
This study aimed to investigate the association of Epstein-Barr virus (EBV) with nuclear respiratory factor 1 (NRF1) and the biological function of NRF1 in EBV-associated gastric cancer (EBVaGC). Western blot and qRT-PCR were used to assess the effect of latent membrane protein 2A (LMP2A) on NRF1 expression after transfection with LMP2A plasmid or siLMP2A. The effects of NRF1 on the migration and apoptosis ability of GC cells were investigated by transwell assay and flow cytometry apoptosis analysis in vitro, respectively. In addition, we determined the regulatory role of NRF1 in EBV latent infection by western blot and dr...
Source: Virus Genes - February 4, 2023 Category: Genetics & Stem Cells Source Type: research
Identification and complete genome sequencing of a novel betapartitivirus naturally infecting the mycorrhizal desert truffle Terfezia claveryi
In this study, we identified and characterized a new partitivirus inhabiting the ascomycete, mycorrhizal desert truffle speciesTerfezia claveryi, and named it “Terfezia claveryi partitivirus 1” (TcPV1). The entire genome of TcPV1, sequenced by both high throughput sequencing of the total dsRNA extracts and by Sanger sequencing of the RLM-RACE PCR products comprised two dsRNA segments of 2404 bp and 2374 bp, respectively. Both dsRNA genome segments h arbored a single open reading frame (ORF), encoding a putative RNA-dependent RNA polymerase (RdRp), and a capsid protein (CP), respectively. The BLASTp search of the RdRp...
Source: Virus Genes - February 3, 2023 Category: Genetics & Stem Cells Source Type: research
Genome sequence analysis of H7N3 subtype avian influenza virus originated from wild birds and its potential infectivity in mice
AbstractIn 2021, an H7N3 avian influenza virus (AIV) was isolated from a mallard in Tianhewan Yellow River National Wetland Park, Ningxia Hui Autonomous Region, China. Sequences analysis showed that this strain received its genes from H7, H6, H5, H3, and H1 AIVs of domestic poultry and wild birds in Asia and Europe. It was mild pathogenicity in mice. These results suggest the importance of continued surveillance of the H7N3 virus to better understand the ecology and evolution of the AIVs in poultry and wild birds and the potential threat to humans. (Source: Virus Genes)
Source: Virus Genes - February 3, 2023 Category: Genetics & Stem Cells Source Type: research
RNA virus discoveries in the electric ant, Wasmannia auropunctata
AbstractDespite being one of the most destructive invasive species of ants, only two natural enemies are known currently forWasmannia auropunctata, commonly known as the electric ant or little fire ant. Because viruses can be effective biological control agents against many insect pests, including ants, a metagenomics/next-generation sequencing approach was used to facilitate discovery of virus sequences from the transcriptomes ofW. auropunctata. Five new and complete positive sense, single-stranded RNA virus genomes, and one new negative sense, single-stranded RNA virus genome were identified, sequenced, and characterized...
Source: Virus Genes - February 2, 2023 Category: Genetics & Stem Cells Source Type: research
Characterization and genome analysis of a novel phage Kayfunavirus TM1
In this study, a novel virulentPodoviridae phageKayfunavirus TM1 infectingEscherichia coli was isolated from pig farm sewage in Guangxi, China. The one-step growth curve with the optimal multiplicity of infection of 0.01 revealed a latent period of 10 min and a burst size of 50 plaque-forming units per cell. The stability test reveals that it is stable from 4 to 60 °C and pH from 3 to 11. The double-stranded DNA genome of phageKayfunavirus TM1 is composed of 39,948 base pairs with a GC content of 50.03%. (Source: Virus Genes)
Source: Virus Genes - January 26, 2023 Category: Genetics & Stem Cells Source Type: research
First complete genome sequence of lumpy skin disease virus directly from a clinical sample in South India
This study reports the first complete genome sequence and analysis of a pathogenic LSD virus (LSDV) from India (LSDV/208/PVNRTVU/2020) obtained by direct sequencing of a suspected clinical sample using Illumina and Nanopore sequencing technologies. The complete genome sequence of LSDV/208/PVNRTVU/2020 is 150445 bp long, codes for 156 putative genes and carries identical 2254 bp inverted terminal repeats at either ends. The unique features reported in the LSDV isolates from the recent outbreaks in Asia, namely, the insertions of 12 nucleotides in the viral G-protein coupled receptor (GPCR) and 27 nucleot ides leading to ...
Source: Virus Genes - January 23, 2023 Category: Genetics & Stem Cells Source Type: research
Identification and characterization of the genome of a papillomavirus from skin lesions of four-toed hedgehogs (Atelerix albiventris)
This study first identified the presence of a novel PV in Japanese four-toed hedgehogs and provided genetic information about this virus. (Source: Virus Genes)
Source: Virus Genes - January 10, 2023 Category: Genetics & Stem Cells Source Type: research
Whole genome sequencing and annotation of a lysogenic phage vB_EcoP_DE5 isolated from donkey-derived Escherichia coli
AbstractA lysogenic phage vB_EcoP_DE5 (hereafter designated DE5) was isolated from donkey-derivedEscherichia coli. The bacteriophage was examined by transmission electron microscopy, and the result showed that DE5 belonged to the genusKuravirus. DE5 was sensitive to changes in temperature and pH, and it could maintain its activity at pH 7 and below 60 ℃. The whole genome sequencing revealed that DE5 had a double-stranded DNA genome of 177, 305 bp with 42.09% G+C content. A total of 126 open reading frames (ORFs) were identified, including functional genes related to phage integration, DNA replication and modification,...
Source: Virus Genes - January 6, 2023 Category: Genetics & Stem Cells Source Type: research
Hantavirus Brno loanvirus is highly specific to the common noctule bat (Nyctalus noctula) and widespread in Central Europe
In conclusion, this study demonstrates a broader distribution of BRNV in common noctules in Central Europe, but at low to moderate prevalence. Additional studies are needed to prove the zoonotic potential of this hantavirus and evaluate its transmission within bat populations. (Source: Virus Genes)
Source: Virus Genes - December 21, 2022 Category: Genetics & Stem Cells Source Type: research
