MicroRNAs miR-18a and miR-452 regulate the replication of enterovirus 71 by targeting the gene encoding VP3
In this study, we established a screening system for miRNAs of target genes to detect the effect of miRNAs on Enterovirus 71 (EV71) replication in rhabdomyosarcoma (RD) cells. A 3’-untranslated region (UTR) dual-luciferase assay was performed to confirm putative miRNA targ ets in EV71 genome. Firstly, 13 fragments of EV71 genome were inserted into the vector pMIR, and luciferase activities were analyzed to identify the putative miRNAs of target genes. The expression of the reporter protein was significantly downregulated in cells transfected with the vector containing gene VP3. Then we screened for miRNAs that might targ...
Source: Virus Genes - May 17, 2021 Category: Genetics & Stem Cells Source Type: research
Identification and whole-genome characterization of a novel Porcine Circovirus 3 subtype b strain from swine populations in Vietnam
In this study, we identified PCV3 infection in aborted fetuses and reported the full-length genome sequence of a PCV3 strain identified from southern Vietnam. The complete genome of this PCV3 strain is 2000 nucleotides in length. We found that it shares 98.5 –99.25% sequence identity with other reference sequences and that it clusters with the PCV3b subtype. Several specific mutated sites were found to be unique to this Vietnamese PCV3b strain, including I14M in the Rep protein and K139R, I150F, and P169T in the Cap protein. The sequence data that hav e been made publically available as part of this study will help inves...
Source: Virus Genes - May 16, 2021 Category: Genetics & Stem Cells Source Type: research
Genotypes of rhinovirus detected among children in two communities of South-West Nigeria
This study determined the genotypes of rhinovirus circulating among children in selected cities in Nigeria. Nasopharyngeal and oropharyngeal samples were carefully collected from children showing signs of respiratory infection in two communities in South-west Nigeria. Polymerase Chain Reaction was used to amplify the hypervariable part of the 5'- non-coding region, the entire viral protein gene 4 and the 5' terminus of the VP2 gene of RV. Nucleotide BLAST and phylogenetic analyses were used to genotype the isolates. Of the samples analysed, 12.7% showed rhinovirus positivity. All the three genotypes of rhinovirus were dete...
Source: Virus Genes - May 14, 2021 Category: Genetics & Stem Cells Source Type: research
First detection and complete genome analysis of the Lyon IARC polyomavirus in China from samples of diarrheic cats
AbstractLyon IARC polyomavirus (LIPyV), a newly discovered polyomavirus (PyV), was first identified in 2017 in human skin samples in the USA. Later, it was detected in several other countries in samples of human and feline origin. Our aim was to find out if the virus occurs in China. To this end, 100 fecal samples were collected from cats with diarrhea in Guangxi Province during 2016 and 2018 and tested with polymerase chain reaction (PCR). Only 2 samples that originated from two related individuals were found to be positive. Based on the sequence identity of the 240-bp PCR products, the two positive samples supposedly con...
Source: Virus Genes - May 10, 2021 Category: Genetics & Stem Cells Source Type: research
A highly virulent canine distemper virus strain isolated from vaccinated mink in China
AbstractAn outbreak of canine distemper in 2017 in mink breeding farms (Shandong province, China) caused severe pneumonia, hardened footpads, and death in more than 5000 vaccinated animals. Sequencing of the hemagglutinin and fusion protein genes from the WH2 canine distemper virus (CDV) strain we isolated from the infected minks were clustered into the recently isolated CDV Asia-1 genotype group. The WH2 strain was distinct from the current vaccine strains, containing a novel potential N-glycosylation site in its hemagglutinin protein. It also contained amino acid mutations in the fusion protein gene (I87N, T110P and L386...
Source: Virus Genes - May 5, 2021 Category: Genetics & Stem Cells Source Type: research
Anguillid herpesvirus 1 (AngHV) ORF 95 encodes a late, structural envelope protein
In this study, AngHVORF95 was characterized. Bioinformatics analysis showed thatORF95 putatively encodes a structural protein that is homologous to hemagglutinin-esterase (HE) protein of infectious salmon anemia virus (ISAV). Temporal transcription and expression analysis indicated thatORF95 is a viral late gene. Subcellular localization analysis revealed that ORF95 was predominantly localized in the cytoplasm. Further, western blot analysis indicated that ORF95 is a structural protein of virion envelope. These results provide a novel basis to make further efforts to clarify the function of ORF95 in the process of AngHV in...
Source: Virus Genes - April 30, 2021 Category: Genetics & Stem Cells Source Type: research
Complete genome sequences of novel Berlinvirus and novel Certrevirus lytic for Pectobacterium sp. causing soft rot and black leg disease of potato
AbstractTwo novel dsDNA bacteriophages namedPectobacterium virus CB251 (PcCB251) andPectobacterium virus CB7V (PcCB7V) targeting plant pathogenPectobacterium parmentieri have been isolated and sequenced. The PcCB251 genome consists of 40,557 bp with G+C content of 48.6% and contains 47 predicted genes on a single strand. The phage is classified in genusBerlinvirus, familyAutographiviridae. The PcCB7V phage has a circular dsDNA genome of 146,054 bp with G+C content of 50.4% and contains 269 predicted protein genes on both strands and 13 tRNA genes. The PcCB7V phage can be classified in genusCertrevirus, subfamilyVequint...
Source: Virus Genes - April 29, 2021 Category: Genetics & Stem Cells Source Type: research
Genome characterization of parsley severe stunt-associated virus in Iran
AbstractParsley severe stunt-associated virus (PSSaV) is a recently identified nanovirus first reported in Germany. During a survey for identification of nanoviruses infecting apiaceous plants in south-eastern Iran, PSSaV was identified and characterized using a combination of rolling circle amplification (RCA) and high-throughput sequencing. Parsley plant samples were collected from vegetable production farms in Kerman province. From two symptomatic samples (39Ba and 40Ba), seven PSSaV components (DNA-C, -S, -M, -R, -N, -U1 and -U2) with two phylogenetically distinct variants of DNA-R (R1 and R2) were identified. In commo...
Source: Virus Genes - April 21, 2021 Category: Genetics & Stem Cells Source Type: research
Detection of mink astrovirus in Poland and further phylogenetic comparison with other European and Canadian astroviruses
AbstractMink astrovirus infection remains a poorly understood disease entity, and the aetiological agent itself causes disease with a heterogeneous course, including gastrointestinal and neurological symptoms. This paper presents cases of astrovirus infection in mink from continental Europe. RNA was isolated from the brains and intestines of animals showing symptoms typical of shaking mink syndrome (n = 6). RT-PCR was used to amplify astrovirus genetic material, and the reaction products were separated on a 1% agarose gel. The specificity of the reaction was confirmed by sequencing fragment coding RdRP protein (length ...
Source: Virus Genes - April 15, 2021 Category: Genetics & Stem Cells Source Type: research
Isolation and genetic characterization of SARS-CoV-2 from Indian patients in a single family without H/O travel abroad
AbstractIn view of the rapidly progressing COVID-19 pandemic, our aim was to isolate and characterize SARS-CoV-2 from Indian patients. SARS-CoV-2 was isolated from nasopharyngeal swabs collected from the two members of a family without any history of (H/O) travel abroad. Both the virus isolates (8003 and 8004) showed CPE on day 3 post-inoculation, viral antigens by immunofluorescence assay and produced distinct, clear and uniform plaques. Infectious virus titers were 5 × 106 and 4 × 106 Pfu/ml by plaque assay and 107.5 and 107 by CPE-based TCID50/ml, respectively. Phylogenetic analysis grouped our isolates with...
Source: Virus Genes - March 8, 2021 Category: Genetics & Stem Cells Source Type: research
The host cellular protein Ndufaf4 interacts with the vesicular stomatitis virus M protein and affects viral propagation
AbstractVesicular stomatitis virus (VSV) is an archetypal member of Mononegavirales which causes important diseases in cattle, horses and pigs. The matrix protein (M) of VSV plays critical roles in the replication, assembly/budding and pathogenesis of VSV. To further investigate the role of M during viral growth, we used a two-hyb rid system to screen for host factors that interact with the M protein. Here, NADH: ubiquinone oxidoreductase complex assembly factor 4 (Ndufaf4) was identified as an M-binding partner, and this interaction was confirmed by yeast cotransformation and GST pulldown assays. The globular domain ...
Source: Virus Genes - February 26, 2021 Category: Genetics & Stem Cells Source Type: research
Molecular characterization of poleroviruses isolated from oilseed rape in Greece
AbstractIn 2018 virus-like symptoms, typical of polerovirus infection were observed in several oilseed rape crops in northern Greece. In order to identify the etiological agent of these symptoms a polerovirus-generic RT-PCR assay was applied. Sequencing of the amplicons revealed the presence of virus isolates genetically close to turnip yellows virus (TuYV). Further molecular characterization of the near complete genome of ‘1–2’, ‘Geo1’, ‘Geo7’ and ‘Geo15’ isolates revealed that they share > 96% nt identity with various TuYV sequences. On the other hand, the fifth, characterized isolate from oilse...
Source: Virus Genes - February 25, 2021 Category: Genetics & Stem Cells Source Type: research
Profiling of alternative polyadenylation and gene expression in PEDV-infected IPEC-J2 cells
In this study, we used in vitro transcription sequencing alternative polyadenylation sites (IVT-SAPAS) method, which allowed accurate profi ling of gene expression and alternative polyadenylation (APA) sites to profile APA switching genes and differentially expressed genes (DEGs) in IPEC-J2 cells during PEDV variant strain infection. We found 804 APA switching genes, including switching in tandem 3′ UTRs and switching between coding r egion and 3′ UTR, and 1,677 DEGs in host after PEDV challenge. These genes participated in variety of biological processes such as cellular process, metabolism and immunity reactions. Mor...
Source: Virus Genes - February 23, 2021 Category: Genetics & Stem Cells Source Type: research
The effect of single amino acid substitution at position 220 in the hemagglutinin glycoprotein on avian influenza H7N9 candidate vaccine virus
In conclusion, amino acid substitutions at posit ion 220 can substantially influence viral biological properties. (Source: Virus Genes)
Source: Virus Genes - February 19, 2021 Category: Genetics & Stem Cells Source Type: research
Comparison of two Turnip mosaic virus P1 proteins in their ability to co-localize with the Arabidopsis thaliana G3BP-2 protein
AbstractTurnip mosaic virus (TuMV), belonging to the genus Potyvirus (familyPotyviridae), has a large host range and consists of a single-stranded positive sense RNA genome encoding 12 proteins, including the P1 protease. This protein which is separated from the polyprotein by cis cleavage at its respective C-terminus, has been attributed with different functions during potyviral infection of plants. P1 of Turnip mosaic virus (P1-TuMV) harbors an FGSF-motif and FGSL-motif at its N-terminus. This motif is predicted to be a binding site for the host Ras GTPase-activating protein-binding protein (G3BP), which is a key factor ...
Source: Virus Genes - February 18, 2021 Category: Genetics & Stem Cells Source Type: research
