Biological characteristics and genomic analysis of a novel Escherichia phage Kayfunavirus CY1
In this study,Escherichia phageKayfunavirus CY1 was isolated from sewage samples of swine farms and identified by biological characteristics and genomic analysis. One-step growth curve showed that the latent period of phage CY1 was about 10 min, the outbreak period was about 40 min and the burst size was 35 PFU/cell. Analysis of the electron microscopy and whole-genome sequence showed that the phage should be classified as a member of theAutographiviridae family,Studiervirinae subfamily. Genomic analysis of phage CY1 (GenBank accession no. OM937123) revealed a genome size of 39,173 bp with an average GC content of 50....
Source: Virus Genes - May 11, 2023 Category: Genetics & Stem Cells Source Type: research
Whole genome sequencing and evolution analyses of Human metapneumovirus
In this study, we sequenced HMPV whole genomes to improve the identification of molecular epidemiology in Beijing, China. Nasopharyngeal aspirates of hospitalized children aged < 14 years old with ARTIs were screened for HMPV infection using qPCR. Fourteen pairs of overlapping primers were used to amplify whole genome sequences of HMPV from positive samples with high viral loads. The epidemiology of HMPV was analysed and 27 HMPV whole genome sequences were obtained. Sequ ence identity and the positional entropy analyses showed that most regions of HMPV genome are conserved, whereas the G gene contained many varia...
Source: Virus Genes - May 7, 2023 Category: Genetics & Stem Cells Source Type: research
Immune response induced by recombinant pres2/S-protein and a pres2-S-protein fused with a core 18-27 antigen fragment of hepatitis B virus compared to conventional HBV vaccine
AbstractAlthough comprehensive vaccination is the cornerstone of public health programs to control hepatitis B virus (HBV) infections, 5% of people who receive the existing vaccine do not develop proper immunity against HBV. To overcome this challenge, researchers have tried using various protein fragments encoded by the virus genome to achieve better immunization rates. An important antigenic component of HBsAg called the preS2/S or M protein has also received much attention in this area. The gene sequences of preS2/S and Core18-27 peptide were extracted from the GenBank (NCBI). Final gene synthesis was conducted with pET...
Source: Virus Genes - May 4, 2023 Category: Genetics & Stem Cells Source Type: research
Co-circulation of two Mumps virus genotypes in Assam, India
AbstractMumps is a vaccine-preventable disease, and research on the vaccine's efficacy has recently indicated declining efficacy that has failed to protect against primary infections or reinfections, leading to a global resurgence in nations that use mumps vaccine in their national immunization programmes (NIPs). Lack of reports on its infection, documentation and published studies prevents it from being recognized as a public health issue in India. The waning of immunity is ascribed to the changes between the circulating and vaccine strains. The goal of the current study was to describe the circulating MuV strains in the ...
Source: Virus Genes - May 3, 2023 Category: Genetics & Stem Cells Source Type: research
Correction to: Characterization of the first Rubus yellow net virus genome from blackberry
(Source: Virus Genes)
Source: Virus Genes - May 3, 2023 Category: Genetics & Stem Cells Source Type: research
Complete genome sequence analysis, morphology and structural protein identification of two Bacillus subtilis phages, BSTP4 and BSTP6, which may form a new species in the genus Salasvirus
AbstractIn the present study, two newBacillus subtilis phages, BSTP4 and BSTP6, were isolated and studied further. Morphologically, BSTP4 and BSTP6 are podoviruses with complete genome of 19,145 (39.9% G + C content) and 19,367 bp (39.8% G + C content), respectively, which became among the smallestBacillus phages. Three most prominent structural proteins were separated and identified as pre-neck appendage, major head, and head fiber proteins using LC –MS/MS. Both phages encode putative terminal proteins (TP) and contain short inverted terminal repeats (ITRs) which may be important for their replication. In addi...
Source: Virus Genes - April 29, 2023 Category: Genetics & Stem Cells Source Type: research
Establishment of reverse genetics for genotype VII Newcastle disease virus and altering the cell tropism by inserting TMPRSS2 into the viral genome
In this study, an NDV strain SD19 (GenBank accession number OP797800) was isolated, and phylogenetic analysis suggested the virus belongs to the class II genotype VII. After generating wild-type rescued SD19 (rSD19), the attenuating strain (raSD19) was generated by mutating the F protein cleavage site. To explore the potential role of the transmembrane protease, serine S1 member 2 (TMPRSS2), the TMPRSS2 gene was inserted into the region between the P and M genes of raSD19 to generate raSD19-TMPRSS2. Besides, the coding sequence of the enhanced green fluorescent protein (EGFP) gene was inserted in the same region as a contr...
Source: Virus Genes - April 27, 2023 Category: Genetics & Stem Cells Source Type: research
Genetic characteristics of a novel HIV-1 recombinant lineage (CRF103_01B) and its prevalence in northern China
AbstractDuring the routine surveillance of HIV-1 pretreatment drug resistance in Beijing, five men who have sex with men (MSM) and a woman were observed to get infected by newly identified CRF103_01B strain. To elucidate the genetic characteristics, the near full-length genome (NFLG) was obtained. Phylogenetic inference indicated that CRF103_01B NFLG was composed of six mosaic segments. Segments IV and V of CRF103_01B were located among the clusters subtype B and CRF01_AE (group 5), respectively. The CRF103_01B strain was deduced to originate from Beijing MSM population around 2002.3 –2006.4 and continued to spread among...
Source: Virus Genes - April 20, 2023 Category: Genetics & Stem Cells Source Type: research
Characterization of Phietavirus Henu 2 in the virome of individuals with acute gastroenteritis
In this study, we characterized a new member (named PhiV_005_BRA/2016) of the recently identified phage speciesPhietavirus Henu 2. PhiV_005_BRA/2016 was detected through metagenomic analysis of stool samples of individuals with acute gastroenteritis. PhiV_005_BRA/2016 contains double-stranded linear DNA (dsDNA), it has a genome of 43,513 base pairs (bp), with a high identity score (99%) with phage of the genusPhietavirus, species ofPhietavirus Henu 2. Life style prediction indicated that PhiV_005_BRA/2016 is a lysogenic phage whose the main host is methicillin-resistantStaphylococcus aureus (MRSA). Indeed, we found PhiV_00...
Source: Virus Genes - April 1, 2023 Category: Genetics & Stem Cells Source Type: research
Subregional origins of emerging SARS-CoV-2 variants during the second pandemic wave in C ôte d’Ivoire
AbstractSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants with increased transmissibility, virulence and immune escape abilities have heavily altered the COVID-19 pandemic ’s course. Deciphering local and global transmission patterns of those variants is thus key in building a profound understanding of the virus’ spread around the globe. In the present study, we investigate SARS-CoV-2 variant epidemiology in Côte d’Ivoire, Western sub-Saharan Africa. We therefo re generated 234 full SARS-CoV-2 genomes stemming from Central and Northern Côte d’Ivoire. Covering the first and second pandemic wave...
Source: Virus Genes - March 18, 2023 Category: Genetics & Stem Cells Source Type: research
Complete genome sequence analysis and biological characteristics of Newcastle disease viruses from different hosts in China
AbstractNewcastle disease (ND) is one of the most serious diseases affecting poultry worldwide. In 2022, we studied two strains of Newcastle disease virus (NDV) from pigeons and magpies identified by PCR and propagated in SPF chicken embryos. The whole genome of the virus was then expanded and its biological characteristics were studied. The results showed that NDV was isolated from pigeons and magpies. Virus present in the allantoic fluid could agglutinate red blood cells and could not be neutralized by serum positive for avian influenza. Sequencing showed that the gene length of the two isolates was 15,191 bp, had high...
Source: Virus Genes - March 16, 2023 Category: Genetics & Stem Cells Source Type: research
Expression and localization of Bombyx mori nucleopolyhedrovirus GP37
This study described the involvement of Bombyx mori nucleopolyhedrovirus (BmNPV) GP37 (BmGP37) in host mitochondria. Herein, the proteins associated with host mitochondria isolated from BmNPV-infected or mock-infected cells by two-dimensional gel electrophoresis were compared. One mitochondria-associated protein in virus-infected cells was identified as BmGP37 by liquid chromatography-mass spectrometry analysis. Furthermore, the BmGP37 antibodies were generated, which could react specifically with BmGP37 in the BmNPV-infected BmN cells. Western blot experiments showed that BmGP37 was expressed at 18 h post-infection and ...
Source: Virus Genes - March 13, 2023 Category: Genetics & Stem Cells Source Type: research
The emergence of novel Iranian variants in sheeppox and goatpox viral envelope proteins with remarkably altered putative binding affinities with the host receptor
The objective of this study was to predict the impacts of the SGP P32/envelope variations on the binding with host receptors as a candidate tool to assess this outbreak. The targeted gene was amplified in a total of 101 viral samples, and the PCR products were subjected to Sanger sequencing. The polymorphism and phylogenetic interactions of the identified variants were assessed. Molecular docking was performed between the identified P32 variants and the host receptor and the effects of these variants were evaluated. Eighteen variations were identified in the investigated P32 gene with variable silent and missense effects o...
Source: Virus Genes - March 13, 2023 Category: Genetics & Stem Cells Source Type: research
Comprehensive analysis of clinical indications and viral strain variants among patients infected with SARS-CoV-2 in Inner Mongolia, China
In this study, we collected 6 throat swabs from patients who diagnosed with COVID-19 in Inner Mongolia, China, to understand the entry of multiple SARS-CoV-2 variants into Inner Mongolia and analyze the relationships between variants and clinical features observed in infected patients. In addition, we performed a combined analysis of clinical parameters associated with SARS-CoV-2 variants of interest, pedigree analysis, and detection of single-nucleotide polymorphisms. Our results showed that the clinical symptoms were generally mild although some patients demonstrated some degree of liver function abnormalities, and the S...
Source: Virus Genes - March 11, 2023 Category: Genetics & Stem Cells Source Type: research
Characterization and phylogenetic analysis of bovine gammaherpesvirus 4 isolated in China, 2022
This study will provide foundation for epidemiological investigations of BoHV-4 and contribute to the molecular and pathogenic studies of BoHV-4. (Source: Virus Genes)
Source: Virus Genes - March 6, 2023 Category: Genetics & Stem Cells Source Type: research
