2,3,7,8-Tetrachlorodibenzo-p-dioxin and kynurenine induce Parkin expression in neuroblastoma cells through different signaling pathways mediated by the aryl hydrocarbon receptor
We describe here the molecular mechanism of AHR-mediated Parkin regulation in human SH-SY5Y neuroblastoma cells. Specifically, we report that the human Parkin gene (PRKN) is transcriptionally upregulated by the aryl hydrocarbon receptor (AHR) through two different selective ligand-dependent pathways. 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), a stress-inducing AHR ligand, indirectly promotes PRKN transcription by inducing ATF4 expression via TCDD-mediated endoplasmic reticulum (ER) stress. In contrast, kynurenine, a nontoxic AHR agonist, induces PRKN transcription by promoting AHR binding to the PRKN promoter without acti...
Source: Toxicology Letters - March 4, 2024 Category: Toxicology Authors: F átima E Murillo-González Rosario Garc ía-Aguilar Jorge Lim ón-Pacheco Mar ía Cabañas-Cortés Guillermo Elizondo Source Type: research
2,3,7,8-Tetrachlorodibenzo-p-dioxin and kynurenine induce Parkin expression in neuroblastoma cells through different signaling pathways mediated by the aryl hydrocarbon receptor
We describe here the molecular mechanism of AHR-mediated Parkin regulation in human SH-SY5Y neuroblastoma cells. Specifically, we report that the human Parkin gene (PRKN) is transcriptionally upregulated by the aryl hydrocarbon receptor (AHR) through two different selective ligand-dependent pathways. 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), a stress-inducing AHR ligand, indirectly promotes PRKN transcription by inducing ATF4 expression via TCDD-mediated endoplasmic reticulum (ER) stress. In contrast, kynurenine, a nontoxic AHR agonist, induces PRKN transcription by promoting AHR binding to the PRKN promoter without acti...
Source: Toxicology Letters - March 4, 2024 Category: Toxicology Authors: F átima E Murillo-González Rosario Garc ía-Aguilar Jorge Lim ón-Pacheco Mar ía Cabañas-Cortés Guillermo Elizondo Source Type: research
2,3,7,8-Tetrachlorodibenzo-p-dioxin and kynurenine induce Parkin expression in neuroblastoma cells through different signaling pathways mediated by the aryl hydrocarbon receptor
We describe here the molecular mechanism of AHR-mediated Parkin regulation in human SH-SY5Y neuroblastoma cells. Specifically, we report that the human Parkin gene (PRKN) is transcriptionally upregulated by the aryl hydrocarbon receptor (AHR) through two different selective ligand-dependent pathways. 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), a stress-inducing AHR ligand, indirectly promotes PRKN transcription by inducing ATF4 expression via TCDD-mediated endoplasmic reticulum (ER) stress. In contrast, kynurenine, a nontoxic AHR agonist, induces PRKN transcription by promoting AHR binding to the PRKN promoter without acti...
Source: Toxicology Letters - March 4, 2024 Category: Toxicology Authors: F átima E Murillo-González Rosario Garc ía-Aguilar Jorge Lim ón-Pacheco Mar ía Cabañas-Cortés Guillermo Elizondo Source Type: research
Mitochondrial Uptake of Aristolactam I Plays a Critical Role in its Toxicity
In conclusion, this research reports the mitochondrial uptake of ALI and provides new insight on potential strategies for protection against AAI-induced nephrotoxicity.PMID:38428544 | DOI:10.1016/j.toxlet.2024.02.013 (Source: Toxicology Letters)
Source: Toxicology Letters - March 1, 2024 Category: Toxicology Authors: Yan Zhou Ruirui Cui Mingkang Zhang Fabing Tang Xiaohua Ma Xin'an Wu Source Type: research
Development and Characterization of an Automated Behavioral Assessment Platform for the G öttingen Minipig
Toxicol Lett. 2024 Feb 28:S0378-4274(24)00035-3. doi: 10.1016/j.toxlet.2024.02.009. Online ahead of print.ABSTRACTThe Göttingen minipig is fast becoming the standard for assessing dermal chemical hazards because, like most swine, its skin is predictive of human skin response and because this strain's smaller size makes laboratory manipulations and husbandry easier. Unfortunately, standard behavioral tests and apparatus have not been developed for behavioral assessments of this swine strain. Indeed, computer-controlled automated behavioral testing procedures are much needed. The present research advanced this goal by produ...
Source: Toxicology Letters - March 1, 2024 Category: Toxicology Authors: Jeffrey L Langston Todd M Myers Source Type: research
Non-invasive methods of monitoring Fe < sub > 3 < /sub > O < sub > 4 < /sub > magnetic nanoparticle toxicity in human liver HepaRG cells using impedance biosensing and Coherent anti-Stokes Raman spectroscopic (CARS) microscopy
Toxicol Lett. 2024 Feb 28:S0378-4274(24)00037-7. doi: 10.1016/j.toxlet.2024.02.010. Online ahead of print.ABSTRACTFunctionalized nanoparticles have been developed for use in nanomedicines for treating life threatening diseases including various cancers. To ensure safe use of these new nanoscale reagents, various assays for biocompatibility or cytotoxicity in vitro using cell lines often serve as preliminary assessments prior to in vivo animal testing. However, many of these assays were designed for soluble, colourless materials and may not be suitable for coloured, non-transparent nanoparticles. Moreover, cell lines are no...
Source: Toxicology Letters - March 1, 2024 Category: Toxicology Authors: Joel Kuhn Alison McDonald Cyril Mongoin Graham Anderson Guillemette Lafeuillade Stephen Mitchell Alistair P D Elfick Pierre O Bagnaninchi Humphrey H P Yiu Leonard J Nelson Source Type: research
Mitochondrial Uptake of Aristolactam I Plays a Critical Role in its Toxicity
In conclusion, this research reports the mitochondrial uptake of ALI and provides new insight on potential strategies for protection against AAI-induced nephrotoxicity.PMID:38428544 | DOI:10.1016/j.toxlet.2024.02.013 (Source: Toxicology Letters)
Source: Toxicology Letters - March 1, 2024 Category: Toxicology Authors: Yan Zhou Ruirui Cui Mingkang Zhang Fabing Tang Xiaohua Ma Xin'an Wu Source Type: research
Development and Characterization of an Automated Behavioral Assessment Platform for the G öttingen Minipig
Toxicol Lett. 2024 Feb 28:S0378-4274(24)00035-3. doi: 10.1016/j.toxlet.2024.02.009. Online ahead of print.ABSTRACTThe Göttingen minipig is fast becoming the standard for assessing dermal chemical hazards because, like most swine, its skin is predictive of human skin response and because this strain's smaller size makes laboratory manipulations and husbandry easier. Unfortunately, standard behavioral tests and apparatus have not been developed for behavioral assessments of this swine strain. Indeed, computer-controlled automated behavioral testing procedures are much needed. The present research advanced this goal by produ...
Source: Toxicology Letters - March 1, 2024 Category: Toxicology Authors: Jeffrey L Langston Todd M Myers Source Type: research
Non-invasive methods of monitoring Fe < sub > 3 < /sub > O < sub > 4 < /sub > magnetic nanoparticle toxicity in human liver HepaRG cells using impedance biosensing and Coherent anti-Stokes Raman spectroscopic (CARS) microscopy
Toxicol Lett. 2024 Feb 28:S0378-4274(24)00037-7. doi: 10.1016/j.toxlet.2024.02.010. Online ahead of print.ABSTRACTFunctionalized nanoparticles have been developed for use in nanomedicines for treating life threatening diseases including various cancers. To ensure safe use of these new nanoscale reagents, various assays for biocompatibility or cytotoxicity in vitro using cell lines often serve as preliminary assessments prior to in vivo animal testing. However, many of these assays were designed for soluble, colourless materials and may not be suitable for coloured, non-transparent nanoparticles. Moreover, cell lines are no...
Source: Toxicology Letters - March 1, 2024 Category: Toxicology Authors: Joel Kuhn Alison McDonald Cyril Mongoin Graham Anderson Guillemette Lafeuillade Stephen Mitchell Alistair P D Elfick Pierre O Bagnaninchi Humphrey H P Yiu Leonard J Nelson Source Type: research
Mitochondrial Uptake of Aristolactam I Plays a Critical Role in its Toxicity
In conclusion, this research reports the mitochondrial uptake of ALI and provides new insight on potential strategies for protection against AAI-induced nephrotoxicity.PMID:38428544 | DOI:10.1016/j.toxlet.2024.02.013 (Source: Toxicology Letters)
Source: Toxicology Letters - March 1, 2024 Category: Toxicology Authors: Yan Zhou Ruirui Cui Mingkang Zhang Fabing Tang Xiaohua Ma Xin'an Wu Source Type: research
Development and Characterization of an Automated Behavioral Assessment Platform for the G öttingen Minipig
Toxicol Lett. 2024 Feb 28:S0378-4274(24)00035-3. doi: 10.1016/j.toxlet.2024.02.009. Online ahead of print.ABSTRACTThe Göttingen minipig is fast becoming the standard for assessing dermal chemical hazards because, like most swine, its skin is predictive of human skin response and because this strain's smaller size makes laboratory manipulations and husbandry easier. Unfortunately, standard behavioral tests and apparatus have not been developed for behavioral assessments of this swine strain. Indeed, computer-controlled automated behavioral testing procedures are much needed. The present research advanced this goal by produ...
Source: Toxicology Letters - March 1, 2024 Category: Toxicology Authors: Jeffrey L Langston Todd M Myers Source Type: research
Non-invasive methods of monitoring Fe < sub > 3 < /sub > O < sub > 4 < /sub > magnetic nanoparticle toxicity in human liver HepaRG cells using impedance biosensing and Coherent anti-Stokes Raman spectroscopic (CARS) microscopy
Toxicol Lett. 2024 Feb 28:S0378-4274(24)00037-7. doi: 10.1016/j.toxlet.2024.02.010. Online ahead of print.ABSTRACTFunctionalized nanoparticles have been developed for use in nanomedicines for treating life threatening diseases including various cancers. To ensure safe use of these new nanoscale reagents, various assays for biocompatibility or cytotoxicity in vitro using cell lines often serve as preliminary assessments prior to in vivo animal testing. However, many of these assays were designed for soluble, colourless materials and may not be suitable for coloured, non-transparent nanoparticles. Moreover, cell lines are no...
Source: Toxicology Letters - March 1, 2024 Category: Toxicology Authors: Joel Kuhn Alison McDonald Cyril Mongoin Graham Anderson Guillemette Lafeuillade Stephen Mitchell Alistair P D Elfick Pierre O Bagnaninchi Humphrey H P Yiu Leonard J Nelson Source Type: research
Roles of Breast Cancer Resistance Protein and Organic Anion Transporting Polypeptide 2B1 in Gastrointestinal Toxicity Induced by SN-38 under Inflammatory Conditions
In this study, we investigated the roles of drug transporters involved in transport of SN-38, which is an active metabolite of irinotecan, in the intestine under inflammatory conditions in vitro and determined their functional consequences. The expression alterations of breast cancer resistance protein (BCRP) and organic anion transporting polypeptide (OATP) 2B1 were determined at the mRNA and protein levels, and the subsequent functional alterations were evaluated via an accumulation study with the representative transporter substrates [prazosin and dibromofluorescein (DBF)] and SN-38. We also determined the cytotoxicity ...
Source: Toxicology Letters - February 29, 2024 Category: Toxicology Authors: Aneela Gulnaz Kyeong-Ryoon Lee Min-Ji Kang Ji-Eun Chang Yoon-Jee Chae Source Type: research
A Chirality-Sensitive Approach to Predict Chemical Transfer Across the Human Placental Barrier
In conclusion, we demonstrate the need of a chirality-sensitive approach for modelling placental transfer of chemicals, and present two predictive QSPR models that may reliably be used for prediction of placental transfer.PMID:38423482 | DOI:10.1016/j.toxlet.2024.02.012 (Source: Toxicology Letters)
Source: Toxicology Letters - February 29, 2024 Category: Toxicology Authors: Anish Gomatam Evans Coutinho Source Type: research
Roles of Breast Cancer Resistance Protein and Organic Anion Transporting Polypeptide 2B1 in Gastrointestinal Toxicity Induced by SN-38 under Inflammatory Conditions
In this study, we investigated the roles of drug transporters involved in transport of SN-38, which is an active metabolite of irinotecan, in the intestine under inflammatory conditions in vitro and determined their functional consequences. The expression alterations of breast cancer resistance protein (BCRP) and organic anion transporting polypeptide (OATP) 2B1 were determined at the mRNA and protein levels, and the subsequent functional alterations were evaluated via an accumulation study with the representative transporter substrates [prazosin and dibromofluorescein (DBF)] and SN-38. We also determined the cytotoxicity ...
Source: Toxicology Letters - February 29, 2024 Category: Toxicology Authors: Aneela Gulnaz Kyeong-Ryoon Lee Min-Ji Kang Ji-Eun Chang Yoon-Jee Chae Source Type: research
