Mutations in the SARS-CoV-2 spike receptor binding domain and their delicate balance between ACE2 affinity and antibody evasion
Protein Cell. 2024 Mar 5:pwae007. doi: 10.1093/procel/pwae007. Online ahead of print.ABSTRACTIntensive selection pressure constrains the evolutionary trajectory of SARS-CoV-2 genomes and results in various novel variants with distinct mutation profiles. Point mutations, particularly those within the receptor binding domain (RBD) of SARS-CoV-2 spike (S) protein lead to the functional alteration in both receptor engagement and monoclonal antibody (mAb) recognition. Here, we review the data of the RBD point mutations possessed by major SARS-CoV-2 variants and discuss their individual effects on ACE2 affinity and immune evasio...
Source: Protein and Cell - March 5, 2024 Category: Cytology Authors: Song Xue Yuru Han Fan Wu Qiao Wang Source Type: research
Skeletal stem cells in bone development, homeostasis and disease
Protein Cell. 2024 Mar 5:pwae008. doi: 10.1093/procel/pwae008. Online ahead of print.ABSTRACTTissue-resident stem cells are essential for development and repair, and in the skeleton this function is fulfilled by recently identified skeletal stem cells (SSCs). However, recent work has identified that SSCs are not monolithic, with long bones, craniofacial sites, and the spine being formed by distinct stem cells. Recent studies have utilized techniques such as fluorescence-activated cell sorting (FACS), lineage tracing and single-cell sequencing to investigate the involvement of SSCs in bone development, homeostasis and disea...
Source: Protein and Cell - March 5, 2024 Category: Cytology Authors: Guixin Yuan Xixi Lin Ying Liu Matthew B Greenblatt Ren Xu Source Type: research
Immune rebalancing at the maternal-fetal interface of maternal SARS-CoV-2 infection during early pregnancy
Protein Cell. 2024 Mar 5:pwae006. doi: 10.1093/procel/pwae006. Online ahead of print.ABSTRACTThe current coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus (SARS-CoV-2) remains a threat to pregnant women. However, the impact of early pregnancy SARS-CoV-2 infection on the maternal-fetal interface remains poorly understood. Here, we present a comprehensive analysis of single-cell transcriptomics and metabolomics in placental samples infected with SARS-CoV-2 during early pregnancy. Compared to control placentas, SARS-CoV-2 infection elicited immune responses at the maternal-fe...
Source: Protein and Cell - March 5, 2024 Category: Cytology Authors: Chenxiang Xi Zihui Yan Dandan Bai Yalin Zhang Beiying Wang Xiao Xiao Han Li Wu Xiaohui Shi Zhiyi Hu Ming Tang Zhongqu Su Yingdong Liu Binya Liu Jiqing Yin Hong Wang Xiaocui Li Yanping Zhang Shaorong Gao Wenqiang Liu Source Type: research
Mutations in the SARS-CoV-2 spike receptor binding domain and their delicate balance between ACE2 affinity and antibody evasion
Protein Cell. 2024 Mar 5:pwae007. doi: 10.1093/procel/pwae007. Online ahead of print.ABSTRACTIntensive selection pressure constrains the evolutionary trajectory of SARS-CoV-2 genomes and results in various novel variants with distinct mutation profiles. Point mutations, particularly those within the receptor binding domain (RBD) of SARS-CoV-2 spike (S) protein lead to the functional alteration in both receptor engagement and monoclonal antibody (mAb) recognition. Here, we review the data of the RBD point mutations possessed by major SARS-CoV-2 variants and discuss their individual effects on ACE2 affinity and immune evasio...
Source: Protein and Cell - March 5, 2024 Category: Cytology Authors: Song Xue Yuru Han Fan Wu Qiao Wang Source Type: research
Skeletal stem cells in bone development, homeostasis and disease
Protein Cell. 2024 Mar 5:pwae008. doi: 10.1093/procel/pwae008. Online ahead of print.ABSTRACTTissue-resident stem cells are essential for development and repair, and in the skeleton this function is fulfilled by recently identified skeletal stem cells (SSCs). However, recent work has identified that SSCs are not monolithic, with long bones, craniofacial sites, and the spine being formed by distinct stem cells. Recent studies have utilized techniques such as fluorescence-activated cell sorting (FACS), lineage tracing and single-cell sequencing to investigate the involvement of SSCs in bone development, homeostasis and disea...
Source: Protein and Cell - March 5, 2024 Category: Cytology Authors: Guixin Yuan Xixi Lin Ying Liu Matthew B Greenblatt Ren Xu Source Type: research
Immune rebalancing at the maternal-fetal interface of maternal SARS-CoV-2 infection during early pregnancy
Protein Cell. 2024 Mar 5:pwae006. doi: 10.1093/procel/pwae006. Online ahead of print.ABSTRACTThe current coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus (SARS-CoV-2) remains a threat to pregnant women. However, the impact of early pregnancy SARS-CoV-2 infection on the maternal-fetal interface remains poorly understood. Here, we present a comprehensive analysis of single-cell transcriptomics and metabolomics in placental samples infected with SARS-CoV-2 during early pregnancy. Compared to control placentas, SARS-CoV-2 infection elicited immune responses at the maternal-fe...
Source: Protein and Cell - March 5, 2024 Category: Cytology Authors: Chenxiang Xi Zihui Yan Dandan Bai Yalin Zhang Beiying Wang Xiao Xiao Han Li Wu Xiaohui Shi Zhiyi Hu Ming Tang Zhongqu Su Yingdong Liu Binya Liu Jiqing Yin Hong Wang Xiaocui Li Yanping Zhang Shaorong Gao Wenqiang Liu Source Type: research
Mutations in the SARS-CoV-2 spike receptor binding domain and their delicate balance between ACE2 affinity and antibody evasion
Protein Cell. 2024 Mar 5:pwae007. doi: 10.1093/procel/pwae007. Online ahead of print.ABSTRACTIntensive selection pressure constrains the evolutionary trajectory of SARS-CoV-2 genomes and results in various novel variants with distinct mutation profiles. Point mutations, particularly those within the receptor binding domain (RBD) of SARS-CoV-2 spike (S) protein lead to the functional alteration in both receptor engagement and monoclonal antibody (mAb) recognition. Here, we review the data of the RBD point mutations possessed by major SARS-CoV-2 variants and discuss their individual effects on ACE2 affinity and immune evasio...
Source: Protein and Cell - March 5, 2024 Category: Cytology Authors: Song Xue Yuru Han Fan Wu Qiao Wang Source Type: research
Skeletal stem cells in bone development, homeostasis and disease
Protein Cell. 2024 Mar 5:pwae008. doi: 10.1093/procel/pwae008. Online ahead of print.ABSTRACTTissue-resident stem cells are essential for development and repair, and in the skeleton this function is fulfilled by recently identified skeletal stem cells (SSCs). However, recent work has identified that SSCs are not monolithic, with long bones, craniofacial sites, and the spine being formed by distinct stem cells. Recent studies have utilized techniques such as fluorescence-activated cell sorting (FACS), lineage tracing and single-cell sequencing to investigate the involvement of SSCs in bone development, homeostasis and disea...
Source: Protein and Cell - March 5, 2024 Category: Cytology Authors: Guixin Yuan Xixi Lin Ying Liu Matthew B Greenblatt Ren Xu Source Type: research
Immune rebalancing at the maternal-fetal interface of maternal SARS-CoV-2 infection during early pregnancy
Protein Cell. 2024 Mar 5:pwae006. doi: 10.1093/procel/pwae006. Online ahead of print.ABSTRACTThe current coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus (SARS-CoV-2) remains a threat to pregnant women. However, the impact of early pregnancy SARS-CoV-2 infection on the maternal-fetal interface remains poorly understood. Here, we present a comprehensive analysis of single-cell transcriptomics and metabolomics in placental samples infected with SARS-CoV-2 during early pregnancy. Compared to control placentas, SARS-CoV-2 infection elicited immune responses at the maternal-fe...
Source: Protein and Cell - March 5, 2024 Category: Cytology Authors: Chenxiang Xi Zihui Yan Dandan Bai Yalin Zhang Beiying Wang Xiao Xiao Han Li Wu Xiaohui Shi Zhiyi Hu Ming Tang Zhongqu Su Yingdong Liu Binya Liu Jiqing Yin Hong Wang Xiaocui Li Yanping Zhang Shaorong Gao Wenqiang Liu Source Type: research
Mutations in the SARS-CoV-2 spike receptor binding domain and their delicate balance between ACE2 affinity and antibody evasion
Protein Cell. 2024 Mar 5:pwae007. doi: 10.1093/procel/pwae007. Online ahead of print.ABSTRACTIntensive selection pressure constrains the evolutionary trajectory of SARS-CoV-2 genomes and results in various novel variants with distinct mutation profiles. Point mutations, particularly those within the receptor binding domain (RBD) of SARS-CoV-2 spike (S) protein lead to the functional alteration in both receptor engagement and monoclonal antibody (mAb) recognition. Here, we review the data of the RBD point mutations possessed by major SARS-CoV-2 variants and discuss their individual effects on ACE2 affinity and immune evasio...
Source: Protein and Cell - March 5, 2024 Category: Cytology Authors: Song Xue Yuru Han Fan Wu Qiao Wang Source Type: research
Skeletal stem cells in bone development, homeostasis and disease
Protein Cell. 2024 Mar 5:pwae008. doi: 10.1093/procel/pwae008. Online ahead of print.ABSTRACTTissue-resident stem cells are essential for development and repair, and in the skeleton this function is fulfilled by recently identified skeletal stem cells (SSCs). However, recent work has identified that SSCs are not monolithic, with long bones, craniofacial sites, and the spine being formed by distinct stem cells. Recent studies have utilized techniques such as fluorescence-activated cell sorting (FACS), lineage tracing and single-cell sequencing to investigate the involvement of SSCs in bone development, homeostasis and disea...
Source: Protein and Cell - March 5, 2024 Category: Cytology Authors: Guixin Yuan Xixi Lin Ying Liu Matthew B Greenblatt Ren Xu Source Type: research
Microbiota enterotoxigenic Bacteroides fragilis-secreted BFT-1 promotes breast cancer cell stemness and chemoresistance through its functional receptor NOD1
Protein Cell. 2024 Mar 4:pwae005. doi: 10.1093/procel/pwae005. Online ahead of print.ABSTRACTTumor-resident microbiota in breast cancer promote cancer initiation and malignant progression. However, targeting microbiota to improve the effects of breast cancer therapy has not been investigated in detail. Here, we evaluated the microbiota composition of breast tumors and found that enterotoxigenic Bacteroides fragilis (ETBF) was highly enriched in the tumors of patients who did not respond to taxane-based neoadjuvant chemotherapy. ETBF, albeit at low biomass, secreted the toxic protein BFT-1 to promote breast cancer cell stem...
Source: Protein and Cell - March 4, 2024 Category: Cytology Authors: Wei Ma Lu Zhang Weilong Chen Zhaoxia Chang Juchuanli Tu Yuanyuan Qin Yuwen Yao Mengxue Dong Jiajun Ding Siqin Li Fengkai Li Qiaodan Deng Yifei Yang Tingting Feng Fanrong Zhang Xiying Shao Xueyan He Lixing Zhang Guohong Hu Quentin Liu Yi-Zhou Jiang Shu Zhu Source Type: research
Proteomic analysis of ferroptosis pathways reveals a role of CEPT1 in suppressing ferroptosis
In this study, we established a protein interaction landscape for ferroptosis pathways through proteomic analyses, and identified choline/ethanolamine phosphotransferase 1 (CEPT1) as a lysophosphatidylcholine acyltransferase 3 (LPCAT3)-interacting protein that regulates LPCAT3 protein stability. In contrast to its known role in promoting phospholipid synthesis, we showed that CEPT1 suppresses ferroptosis potentially by interacting with phospholipases and breaking down certain pro-ferroptotic polyunsaturated fatty acid (PUFA)-containing phospholipids. Together, our study reveals a previously unrecognized role of CEPT1 in su...
Source: Protein and Cell - March 2, 2024 Category: Cytology Authors: Xiaoguang Liu Zhen Chen Yuelong Yan Fereshteh Zandkarimi Litong Nie Qidong Li Amber Horbath Kellen Olszewski Lavanya Kondiparthi Chao Mao Hyemin Lee Li Zhuang Masha Poyurovsky Brent R Stockwell Junjie Chen Boyi Gan Source Type: research
Metabolic cell death in cancer: Ferroptosis, cuproptosis, disulfidptosis, and beyond
Protein Cell. 2024 Mar 1:pwae003. doi: 10.1093/procel/pwae003. Online ahead of print.ABSTRACTCell death resistance represents a hallmark of cancer. Recent studies have identified metabolic cell death as unique forms of regulated cell death resulting from an imbalance in the cellular metabolism. This review discusses the mechanisms of metabolic cell death-ferroptosis, cuproptosis, disulfidptosis, lysozincrosis, and alkaliptosis-and explores their potential in cancer therapy. Our review underscores the complexity of the metabolic cell death pathways and offers insights into innovative therapeutic avenues for cancer treatment...
Source: Protein and Cell - March 1, 2024 Category: Cytology Authors: Chao Mao Min Wang Li Zhuang Boyi Gan Source Type: research
