Activity of Ligustrum robustum (Roxb.) Blume extract against the biofilm formation and exopolysaccharide synthesis of Streptococcus mutans
The extract ofLigustrum robustum (Roxb.) Blume, mainly composed of Ligurobustosides, exhibits antimicrobial activity against oral pathogenStreptococcus mutans. AbstractLigustrum robustum (Roxb.) Blume is utilized as a traditional Chinese herbal tea with various health benefits and protective effects.Streptococcus mutans is an important cariogenic oral bacteria species. The present study aimed to assess the influence ofLigustrum robustum extract (LRE) on the biofilm formation ofS.mutans and the mechanism of its action, as well as to identify its chemical components. For chemical identification, HPLC ‐MS and nuclear magnet...
Source: Molecular Oral Microbiology - December 31, 2020 Category: Microbiology Authors: Zhong Zhang,
Jumei Zeng,
Xuedong Zhou,
Qianda Xu,
Chenghui Li,
Yiduo Liu,
Chaoliang Zhang,
Liu Wang,
Weicai Zeng,
Yuqing Li Tags: ORIGINAL ARTICLE Source Type: research
Periodontal Pathogens ’ strategies disarm neutrophils to promote dysregulated inflammation
Neutrophils normally play a critical role in maintaining homeostasis in the oral cavity, but both established and emerging periodontal pathogens evade and manipulate neutrophil functional mechanisms to initiate and maintain a cycle of inflammation that results in periodontitis. Here, we review the effect of established and emerging periodontal pathogens on each neutrophil function and link the aberrant neutrophil phenotype to the pathogenesis of periodontitis. AbstractPeriodontitis is an irreversible, chronic inflammatory disease where inflammophilic pathogenic microbial communities accumulate in the gingival crevice. Neut...
Source: Molecular Oral Microbiology - December 31, 2020 Category: Microbiology Authors: Irina Miralda,
Silvia M. Uriarte Tags: REVIEW ARTICLE Source Type: research
PG1659 functions as anti ‐sigma factor to extracytoplasmic function sigma factor RpoE in Porphyromonas gingivalis W83
SummaryAnti ‐sigma factors play a critical role in regulating the expression of sigma factors in response to environmental stress signals.PG1659 is cotranscribed with an upstream genePG1660 (rpoE), which encodes for a sigma factor that plays an important role in oxidative stress resistance and the virulence regulatory network ofP. gingivalis.PG1659, which is annotated as a hypothetical gene, is evaluated in this study. PG1659, composed of 130 amino acids, is predicted to be transmembrane protein with a single calcium (Ca2+) binding site. InP. gingivalis FLL358 ( ΔPG1659::ermF), therpoE gene was highly upregulated compar...
Source: Molecular Oral Microbiology - December 29, 2020 Category: Microbiology Authors: Yuetan Dou,
Hiel Rutanhira,
Norbert Schormann,
Champion Deivanayagam,
Hansel M Fletcher Tags: ORIGINAL ARTICLE Source Type: research
Lactobacilli Postbiotics reduce biofilm formation and alter transcription of virulence genes of Aggregatibacter actinomycetemcomitans
AbstractPeriodontitis is characterized by a dysbiotic microbial community and treatment strategies include the reestablishment of symbiosis by reducing pathogens abundance.Aggregatibacter actinomycetemcomitans (Aa) is frequently associated with rapidly progressing periodontitis. Since the oral ecosystem may be affected by metabolic ‐end‐products of bacteria, we evaluated the effect of soluble compounds released by probiotic lactobacilli, known as postbiotics, on Aa biofilm and expression of virulence‐associated genes. Cell‐free pH‐neutralized supernatants (CFS) ofLactobacillus rhamnosus Lr32,L. rhamnosus HN001,La...
Source: Molecular Oral Microbiology - December 29, 2020 Category: Microbiology Authors: Karin Hitomi Ishikawa,
Manuela Rocha Bueno,
Dione Kawamoto,
Maria Regina Lorenzetti Simionato,
Marcia Pinto Alves Mayer Tags: ORIGINAL ARTICLE Source Type: research
Examination of gene expression in saliva samples from COVID ‐19 patients to study the host defense response against SARS‐CoV‐2 in the oral cavity
(Source: Molecular Oral Microbiology)
Source: Molecular Oral Microbiology - December 22, 2020 Category: Microbiology Authors: Gill Diamond,
Erika L. Figgins,
Tanya Robinson,
Michal Senitko,
George E. Abraham,
Haley B. Williams,
Meredith Sloan,
Anna Owings,
Hannah Laird,
Yilianys Pride,
Kenneth J. Wilson,
Mohammad Hasan,
Adam Parker,
Sarah C. Glover Tags: LETTER TO THE EDITOR Source Type: research
Examination of gene expression in saliva samples from COVID ‐19 patients to study the host defense response against SARS‐CoV‐2 in the oral cavity
Molecular Oral Microbiology, EarlyView. (Source: Molecular Oral Microbiology)
Source: Molecular Oral Microbiology - December 21, 2020 Category: Microbiology Authors: Gill Diamond,
Erika L. Figgins,
Tanya Robinson,
Michal Senitko,
George E. Abraham,
Haley B. Williams,
Meredith Sloan,
Anna Owings,
Hannah Laird,
Yilianys Pride,
Kenneth J. Wilson,
Mohammad Hasan,
Adam Parker,
Sarah C. Glover Tags: LETTER TO THE EDITOR Source Type: research
Aggregatibacter actinomycetemcomitans induces a proatherosclerotic response in human endothelial cells in a three ‐dimensional collagen scaffold model
This study characterizes a three ‐dimensional model with human coronary artery endothelial cells on three‐dimensional (HCAEC‐3D) type I collagen scaffolds to evaluate whether infection withAggregatibacter actinomycetemcomitans induces a proinflammatory response associated with atherosclerosis. The HCAEC ‐3D culture was physicochemically characterized with regard to biocompatibility and barrier function. Then, the culture was infected withA. actinomycetemcomitans strain ATCC 29522 at multiplicities of infection (MOIs) of 1:1, 1:10, and 1:100. Cultures without infection and stimulated withA. actinomycetemcomitans lip...
Source: Molecular Oral Microbiology - December 19, 2020 Category: Microbiology Authors: Maria A. Torres,
Diego F. Gualtero,
Gloria I. Lafaurie,
Marta R. Fontanilla Tags: ORIGINAL ARTICLE Source Type: research
Activity of Ligustrum robustum (Roxb.) Blume extract against the biofilm formation and exopolysaccharide synthesis of Streptococcus mutans
AbstractLigustrum robustum (Roxb.) Blume is utilized as a traditional Chinese herbal tea with various health benefits and protective effects.Streptococcus mutans is an important cariogenic oral bacteria species. The present study aimed to assess the influence ofLigustrum. robustum extract (LRE) on the biofilm formation ofS.mutans and the mechanism of its action, as well as to identify its chemical components. For chemical identification, HPLC ‐MS and NMR were applied and four identified phytochemicals were reported (Ligurobustoside B, Ligurobustoside N, Ligurobustoside J and Ligurobustoside C). The dose‐dependent (0.5 ...
Source: Molecular Oral Microbiology - December 14, 2020 Category: Microbiology Authors: Zhong Zhang,
Jumei Zeng,
Xuedong Zhou,
Qianda Xu,
Chenghui Li,
Yiduo Liu,
Chaoliang Zhang,
Liu Wang,
Weicai Zeng,
Yuqing Li Tags: ORIGINAL ARTICLE Source Type: research
Development of an FhbB based chimeric vaccinogen that elicits antibodies that block Factor H binding and cleavage by the periopathogen Treponema denticola
This study is the first to extend this approach to the development of a preventive or therapeutic vaccine (or monoclonal Ab) for periodontal disease. (Source: Molecular Oral Microbiology)
Source: Molecular Oral Microbiology - December 9, 2020 Category: Microbiology Authors: Nathaniel S. O'Bier,
Dhara T. Patel,
Lee D. Oliver,
Daniel P. Miller,
Richard T. Marconi Tags: ORIGINAL ARTICLE Source Type: research
Examination of gene expression in saliva samples from COVID ‐19 patients to study the host defense response against SARS‐CoV‐2 in the oral cavity
AbstractInfection with severe acute respiratory syndrome coronavirus 2 (SARS ‐CoV‐2) causes coronavirus disease 2019 (COVID‐19), which presents with respiratory symptoms including fever, sore throat, cough and congestion, suggesting that infection of the oral cavity and nasopharynx (ONP) is an obligate step in its pathogenesis. Consistent with this role in the initial infection, oral and nasopharyngeal epithelial cells express the SARS‐CoV‐2 spike protein receptor, angiotensin‐converting enzyme 2 (ACE2) (1), and RT‐PCR based testing can routinely detect SARS‐CoV‐2 RNA within both the nasal and buccal tiss...
Source: Molecular Oral Microbiology - December 8, 2020 Category: Microbiology Authors: Gill Diamond,
Erika L. Figgins,
Tanya Robinson,
Michal Senitko,
George E. Abraham,
Haley B. Williams,
Meredith Sloan,
Anna Owings,
Hannah Laird,
Yilianys Pride,
Kenneth J. Wilson,
Mohammad Hasan,
Adam Parker,
Sarah C. Glover Tags: LETTER TO THE EDITOR Source Type: research
Aggregatibacter actinomycetemcomitans induces a proatherosclerotic response in human endothelial cells in a three ‐dimensional collagen scaffold model
This study characterizes a three ‐dimensional model with human coronary artery endothelial cells on three‐dimensional (HCAEC‐3D) type I collagen scaffolds to evaluate whether infection withAggregatibacter actinomycetemcomitans induces a proinflammatory response associated with atherosclerosis. The HCAEC ‐3D culture was physicochemically characterized in regard to biocompatibility and barrier function. Then, the culture was infected withA. actinomycetemcomitans strain ATCC 29522 at multiplicities of infection (MOIs) of 1:1, 1:10, and 1:100. Cultures without infection and stimulated withA. actinomycetemcomitans lipop...
Source: Molecular Oral Microbiology - December 7, 2020 Category: Microbiology Authors: Maria A. Torres,
Diego F. Gualtero,
Gloria I. Lafaurie,
Marta R. Fontanilla Tags: ORIGINAL ARTICLE Source Type: research
Issue Information
Molecular Oral Microbiology, Volume 35, Issue 6, Page i-iii, December 2020. (Source: Molecular Oral Microbiology)
Source: Molecular Oral Microbiology - December 6, 2020 Category: Microbiology Tags: ISSUE INFORMATION Source Type: research
Identification of Streptococcus mutans genes involved in fluoride resistance by screening of a transposon mutant library
We describe here the construction and characterization of a mariner ‐based transposon system designed to be used inS. mutans, which is also potentially applicable to other streptococci. To identify genetic determinants of fluoride resistance inS. mutans, we constructed a library ofS. mutans transposon insertion mutants and screened this library to identify mutants exhibiting fluoride resistance phenotype. Two mutants were found to carry transposon insertion in two different genetic loci (smu.396 andsmu.1291c), respectively. Our subsequent genetic study indicates the fluoride ‐resistant phenotype for the mutant with the...
Source: Molecular Oral Microbiology - December 6, 2020 Category: Microbiology Authors: Jie Yu,
Yaqi Wang,
Dongmei Han,
Wei Cao,
Lanyan Zheng,
Zhoujie Xie,
Hao Liu Tags: ORIGINAL ARTICLE Source Type: research
Comprehensive profiling of protein lysine acetylation and its overlap with lysine succinylation in the Porphyromonas gingivalis fimbriated strain ATCC 33277
This study provides a significant beginning for further investigating the role of Kac and Ksuc in the pathogenicity ofP. gingivalis. (Source: Molecular Oral Microbiology)
Source: Molecular Oral Microbiology - December 6, 2020 Category: Microbiology Authors: Jumei Zeng,
Leng Wu,
Qiushi Chen,
Lingyun Wang,
Wei Qiu,
Xin Zheng,
Xiaoming Yin,
Jie Liu,
Yan Ren,
Yuqing Li Tags: ORIGINAL ARTICLE Source Type: research
