Nicotinamide employs a starvation strategy against Porphyromonas gingivalis virulence by inhibiting the heme uptake system and gingipain activities
In this study, we investigated the inhibitory effect of nicotinamide (NAM), a vitamin B3 derivative, on the growth and virulence ofPorphyromonas gingivalis, a key member of the red complex. Our findings revealed that NAM inhibited bacterial growth and gingipain activities, which played a dominant role in protein hydrolysis and heme acquisition. NAM decreased hemagglutination and hemolysis abilities and changed hemin and hemoglobin binding capacities, controlling bacterial infection through a starvation strategy by blocking access to growth-essential nutrients from the outside and reducing bacterial virulence. Several exper...
Source: Molecular Oral Microbiology - January 11, 2024 Category: Microbiology Authors: Zixue Lei,
Qizhao Ma,
Yeting Tu,
Yang Qiu,
Tao Gong,
Yongwang Lin,
Xuedong Zhou,
Yuqing Li Tags: ORIGINAL ARTICLE Source Type: research
The ways Fusobacterium nucleatum translocate to breast tissue and contribute to breast cancer development
AbstractBreast cancer is among the most prevalent malignancies in women worldwide. Epidemiological findings suggested that periodontal diseases may be associated with breast cancer, among whichFusobacterium nucleatum is considered an important cross-participant. In this work, we comprehensively summarize the known mechanisms of howF. nucleatum translocates to, colonizes in mammary tumors, and promotes the carcinogenesis. Specifically,F. nucleatum translocates to mammary tissue through the mammary –intestinal axis, direct nipple contact, and hematogenous transmission. Subsequently,F. nucleatum takes advantage of fusobacte...
Source: Molecular Oral Microbiology - January 4, 2024 Category: Microbiology Authors: Xinyu Guo,
Ke Yu,
Ruijie Huang Tags: REVIEW ARTICLE Source Type: research
Issue Information
(Source: Molecular Oral Microbiology)
Source: Molecular Oral Microbiology - January 4, 2024 Category: Microbiology Tags: ISSUE INFORMATION Source Type: research
Activation of liver X receptors suppresses the abundance and osteoclastogenic potential of osteoclast precursors and periodontal bone loss
AbstractLiver-X receptors (LXRs) are essential nuclear hormone receptors involved in cholesterol and lipid metabolism. They are also believed to regulate inflammation and physiological and pathological bone turnover. We have previously shown that infection with the periodontal pathogenPorphyromonas gingivalis (Pg) in mice increases the abundance of CD11b+c-fms+Ly6Chi cells in bone marrow (BM), spleen (SPL), and peripheral blood. These cells also demonstrated enhanced osteoclastogenic activity and a distinctive gene profile following Pg infection. Here, we investigated the role of LXRs in regulating these osteoclast precurs...
Source: Molecular Oral Microbiology - December 19, 2023 Category: Microbiology Authors: Yanfang Zhao,
Kai Yang,
Thalyta Amanda Ferreira,
Xuejia Kang,
Xu Feng,
Jannet Katz,
Suzanne M Michalek,
Ping Zhang Tags: ORIGINAL ARTICLE Source Type: research
Filifactor alocis enhances survival of Porphyromonas gingivalis W83 in response to H2O2 ‐induced stress
The objective of this study is to further determine ifF. alocis, because of its increased resistance to oxidative stress, can affect the survival of other ‘established’ periodontal pathogens under environmental stress conditions typical of the periodontal pocket. Here, we have shown that via their interaction,F.alocis protectsP. gingivalis W83 under H2O2-induced oxidative stress conditions. Transcriptional profiling of the interaction ofF. alocis andP. gingivalis in the presence of H2O2-induced stress revealed the modulation of several genes, including those with ABC transporter and other cellular functions. The ABC tr...
Source: Molecular Oral Microbiology - December 2, 2023 Category: Microbiology Authors: Arunima Mishra,
Yuetan Dou,
Charles Wang,
Hansel M Fletcher Tags: ORIGINAL ARTICLE Source Type: research
Issue Information
(Source: Molecular Oral Microbiology)
Source: Molecular Oral Microbiology - November 29, 2023 Category: Microbiology Tags: ISSUE INFORMATION Source Type: research
Dual function of the O ‐antigen WaaL ligase of Aggregatibacter actinomycetemcomitans
In this study, we have determined that theEscherichia coli waaL ortholog (rflA) does not restore collagen binding of awaaL mutant strain ofA. actinomycetemcomitans but does restore O-PS ligase activity following transformation of a plasmid expressingwaaL. Therefore, a heterologousE. coli expression system was developed constituted of two independently replicating plasmids expressing eitherwaaL oremaA ofA. actinomycetemcomitans to directly demonstrate the necessity of ligase activity for EmaA collagen binding. Proper expression of the protein encoded by each plasmid was characterized, and the individually transformed strain...
Source: Molecular Oral Microbiology - November 9, 2023 Category: Microbiology Authors: David R. Danforth,
Marcella Melloni,
Richard Thorpe,
Avi Cohen,
Richard Voogt,
Jake Tristano,
Keith P. Mintz Tags: ORIGINAL ARTICLE Source Type: research
The potential impact of periodontitis on cerebral small vessel disease
AbstractCerebral small vessel disease (CSVD) is a term used to describe abnormalities in the intracranial microvasculature affecting small arteries, arterioles, capillaries, and venules. The etiology of these conditions is not fully understood but inflammation appears to play a significant role. Periodontal diseases have been associated with conditions such as stroke and dementia, which are clinical consequences of CSVD. Periodontitis is a highly prevalent chronic multifactorial inflammatory disease regulated by the host immune response against pathogenic bacterial colonization around the teeth. The inflammatory response a...
Source: Molecular Oral Microbiology - November 7, 2023 Category: Microbiology Authors: Beatriz Bezerra,
Mark Fisher,
Flavia Q. Pirih,
Ma ísa Casarin Tags: INVITED REVIEW Source Type: research
Primed inflammatory response by fibroblast subset is necessary for proper oral and cutaneous wound healing
AbstractFibroblasts are ubiquitous mesenchymal cells that exhibit considerable molecular and functional heterogeneity. Besides maintaining stromal integrity, oral fibroblast subsets are thought to play an important role in host –microbe interaction during injury repair, which is not well explored in vivo. Here, we characterize a subset of fibroblast lineage labeled by paired-related homeobox-1 promoter activity (Prx1Cre+) in oral mucosa and skin and demonstrate these fibroblasts readily respond to microbial products to facilitate the normal wound healing process. Using a reporter mouse model, we determined that Prx1Cre+ ...
Source: Molecular Oral Microbiology - October 31, 2023 Category: Microbiology Authors: Zhaoxu Chen,
Rahul Debnath,
Ifeoma Chikelu,
Jonathan X. Zhou,
Kang I. Ko Tags: ORIGINAL ARTICLE Source Type: research
Effects of IL ‐34 and anti‐IL‐34 neutralizing mAb on alveolar bone loss in a ligature‐induced model of periodontitis
This study illustrates the role of IL-34 in promoting alveolar bone loss in periodontal lesions and proposes the potential of anti-IL34 monoclonal antibody (mAb)-based therapeutic regimens to suppress alveolar bone loss in periodontitis lesions. (Source: Molecular Oral Microbiology)
Source: Molecular Oral Microbiology - October 30, 2023 Category: Microbiology Authors: Carolina Duarte,
Chiaki Yamada,
Bidii Ngala,
Christopher Garcia,
Juliet Akkaoui,
Maxim Birsa,
Anny Ho,
Amilia Nusbaum,
Hawra AlQallaf,
Vanchit John,
Alexandru Movila Tags: ORIGINAL ARTICLE Source Type: research
Enhanced transformation efficiency in Treponema denticola enabled by SyngenicDNA ‐based plasmids lacking restriction–modification target motifs
AbstractOral spirochetes are among a small group of keystone pathogens contributing to dysregulation of tissue homeostatic processes that leads to breakdown of the tissue and bone supporting the teeth in periodontal disease. Additionally, our group has recently demonstrated thatTreponema are among the dominant microbial genera detected intracellularly in tumor specimens from patients with oral squamous cell carcinoma. While over 60 species and phylotypes of oralTreponema have been detected,T. denticola is one of the few that can be grown in culture and the only one in which genetic manipulation is regularly performed. Thus...
Source: Molecular Oral Microbiology - October 26, 2023 Category: Microbiology Authors: Christopher D. Johnston,
M. Paula Goetting ‐Minesky,
Kelly Kennedy,
Valentina Godovikova,
Sara M. Zayed,
Richard J. Roberts,
J. Christopher Fenno Tags: ORIGINAL ARTICLE Source Type: research
Metabolism of serine/glycine lipids by human gingival cells in culture
This study revealed that treatment of HGF or macrophage (THP1) cells with lipid (L) 654 resulted in breakdown to L342 and s ubsequent release into culture medium. However, L654 was converted only to L567 in gingival keratinocytes. By contrast, L1256 was converted to L654 by fibroblasts and macrophages but no further hydrolysis or release into medium was observed. Gingival keratinocytes showed no hydrolysis of L1256 to sm aller lipid products but because L1256 was not recovered in these cells, it is not clear what hydrolysis products are produced from L1256. Although primary cultures of gingival fibroblasts and macrophages ...
Source: Molecular Oral Microbiology - October 18, 2023 Category: Microbiology Authors: Tyler M. Guido,
Samuel D. Ratcliffe,
Amanda Rahmlow,
Matthew A. Zambrello,
Anthony A. Provates,
Robert B. Clark,
Michael B. Smith,
Frank C. Nichols Tags: ORIGINAL ARTICLE Source Type: research
Cover Image, Volume 38, Issue 5
The cover image is based on the Original ArticleRequirements for anti-aquaporin 5 autoantibody production in a mouse model by Sabin Acharya et al.,https://doi.org/10.1111/omi.12430. (Source: Molecular Oral Microbiology)
Source: Molecular Oral Microbiology - October 13, 2023 Category: Microbiology Tags: FEATURED COVER Source Type: research
Issue Information
(Source: Molecular Oral Microbiology)
Source: Molecular Oral Microbiology - October 13, 2023 Category: Microbiology Tags: ISSUE INFORMATION Source Type: research
Critical review of 16S rRNA gene sequencing workflow in microbiome studies: From primer selection to advanced data analysis
AbstractThe multi-batch reanalysis approach of jointly reevaluating gene/genome sequences from different works has gained particular relevance in the literature in recent years. The large amount of 16S ribosomal ribonucleic acid (rRNA) gene sequence data stored in public repositories and information in taxonomic databases of the same gene far exceeds that related to complete genomes. This review is intended to guide researchers new to studying microbiota, particularly the oral microbiota, using 16S rRNA gene sequencing and those who want to expand and update their knowledge to optimise their decision-making and improve the...
Source: Molecular Oral Microbiology - October 8, 2023 Category: Microbiology Authors: Alba Regueira ‐Iglesias,
Carlos Balsa‐Castro,
Triana Blanco‐Pintos,
Inmaculada Tomás Tags: INVITED REVIEW Source Type: research
