A hunt for OM45 synthetic petite interactions in Saccharomyces cerevisiae reveals a role for Miro GTPase Gem1p in cristae structure maintenance
A screen for mutations inSaccharomyces cerevisiae that are synthetically respiratory deficient with a deletion of theOM45 gene (encoding a major yeast mitochondrial outer membrane protein), led to the isolation of mutations inUGO1 andGEM1. Our results indicate the inner mitochondrial membrane (IMM) as the location of action of Om45 and the Miro GTPase Gem1, and a role for these proteins in the maintenance of IMM morphology and mitochondrial DNA. (A) WT state; (B) TheΔom45 strain has no detectable phenotype; (C) TheΔom45Δgem1 synthetic petite (SPM) strain is respiratory deficient, displays severely disturbed IMM morpholo...
Source: MicrobiologyOpen - October 9, 2021 Category: Microbiology Authors: Antonina Shvetsova,
Ali J. Masud,
Laura Schneider,
Ulrich Bergmann,
Geoffray Monteuuis,
Ilkka J. Miinalainen,
J. Kalervo Hiltunen,
Alexander J. Kastaniotis Tags: ORIGINAL ARTICLE Source Type: research
Identifying the best regimen for primary eradication of Helicobacter pylori: analysis of 240 cases
(Source: MicrobiologyOpen)
Source: MicrobiologyOpen - October 8, 2021 Category: Microbiology Tags: CORRIGENDUM Source Type: research
Polyunsaturated fatty acids cause physiological and behavioral changes in Vibrio alginolyticus and Vibrio fischeri
Bacterial utilization of fatty acids continues to evolve as a practical strategy for environmental adaptation, providing energy currency, ingredients for membrane construction, and cues for behavioral modification. Here, we add two members of the familyVibrionaceae,Vibrio alginolyticus andAliivibrio fischeri, that scavenge a variety of polyunsaturated fatty acids (PUFAs), as evidenced by incorporation into membrane phospholipids and orchestration of phenotypic responses associated with growth, motility, and biofilm formation. Our findings highlight the differential exploitation of PUFAs by two related genera occupying dist...
Source: MicrobiologyOpen - October 7, 2021 Category: Microbiology Authors: David S. Smith,
Carina Houck,
Allycia Lee,
Timothy B. Simmons,
Olivia N. Chester,
Ayanna Esdaile,
Steven J. K. Symes,
David K. Giles Tags: ORIGINAL ARTICLE Source Type: research
Effect of the skincare product on facial skin microbial structure and biophysical parameters: A pilot study
This study aimed to determine the bacterial community structure and skin biophysical parameters following the daily application of a skincare product on the face. Twenty-five Korean women, who used the same skincare product for four weeks participated in the study. During this period, skin hydration, texture, sebum content, and pH were measured, and skin swab samples were collected on the cheeks. The microbiota was analyzed using the MiSeq system. Through these experiments, bacterial diversity in facial skin increased and the microbial community changed after four weeks of skincare product application. The relative abundan...
Source: MicrobiologyOpen - October 7, 2021 Category: Microbiology Authors: Bo Kyoung Hwang,
Sado Lee,
Joonoh Myoung,
Seung Jin Hwang,
Jun Man Lim,
Eui Taek Jeong,
Sun Gyoo Park,
Sung Hun Youn Tags: ORIGINAL ARTICLE Source Type: research
Characterization of methicillin ‐resistant Staphylococcus aureus strains colonizing the nostrils of Spanish children
Conclusionsmethicillin-resistantStaphylococcus aureus nasal colonization in Spanish children is rare, with t002 being the most observed spa type, associated with SCCmec IVc. None of the MRSA strains produced pvl, but up to 30% ofS. aureus strains were positive for tst. (Source: MicrobiologyOpen)
Source: MicrobiologyOpen - October 7, 2021 Category: Microbiology Authors: Federico Rom án,
Ana Mendez‐Echevarria,
Teresa Del Rosal,
Cesar Garcia‐Vera,
Luis Escosa‐Garcia,
Martin Agud,
Fernando Chaves,
José Gutiérrez‐Fernández,
Enrique Ruiz de Gopegui,
Guillermo Ruiz‐Carrascoso,
Maria del Carmen Ruiz‐Ga Tags: ORIGINAL ARTICLE Source Type: research
Nuclear conditions of basidiospores and hyphal cells in the edible mushroom Oudemansiella aparlosarca
The life cycle of the fungusOudemansiella aparlosarca includes homokaryotic and heterokaryotic basidiospores with four nuclear conditions: non-nucleate, mononucleate, binucleate, and multinucleate. The number of binucleate cells was high in heterokaryotic hyphal cells, while that of multinucleated cells was high in homokaryotic hyphal cells. The predominance of binucleate spores might be a result of post-meiotic mitosis. AbstractOudemansiella aparlosarca is an edible mushroom possessing medicinal and health benefits. Although there are studies on the cultivation ofO.aparlosarca, only a few studies have focused on its genet...
Source: MicrobiologyOpen - September 30, 2021 Category: Microbiology Authors: Roy Rebecca,
Qi Gao,
Yujin Cui,
Chengbo Rong,
Yu Liu,
Wensheng Zhao,
Wasantha Kumara,
Shouxian Wang Tags: ORIGINAL ARTICLE Source Type: research
Metagenomic strategies identify diverse integron ‐integrase and antibiotic resistance genes in the Antarctic environment
The objective of this study is to identify and analyze integrons and antibiotic resistance genes (ARGs) in samples collected from diverse sites in terrestrial Antarctica. The metagenomic analysis allowed us to identify novel predictedintI integrases and gene cassettes (GCs), which mostly encode unknown functions. AbstractThe objective of this study is to identify and analyze integrons and antibiotic resistance genes (ARGs) in samples collected from diverse sites in terrestrial Antarctica. Integrons were studied using two independent methods. One involved the construction and analysis ofintI gene amplicon libraries. In addi...
Source: MicrobiologyOpen - September 13, 2021 Category: Microbiology Authors: Ver ónica Antelo,
Matías Giménez,
Gastón Azziz,
Patricia Valdespino‐Castillo,
Luisa I. Falcón,
Lucas A. M. Ruberto,
Walter P. Mac Cormack,
Didier Mazel,
Silvia Batista Tags: ORIGINAL ARTICLE Source Type: research
Issue Information
(Source: MicrobiologyOpen)
Source: MicrobiologyOpen - September 9, 2021 Category: Microbiology Tags: ISSUE INFORMATION Source Type: research
Metabolic potential of the imperfect denitrifier Candidatus Desulfobacillus denitrificans in an anammox bioreactor
The imperfect denitrifierCa. D. denitrificans inhabiting anammox bioreactors can use a range of electron donors for denitrification, including aromatic compounds, glucose, sulfur compounds, and hydrogen. However, our metatranscriptomic analysis suggested that the major electron donors are aromatic compounds, which inhibit anammox activity. AbstractThe imperfect denitrifier,Candidatus (Ca.) Desulfobacillus denitrificans, which lacks nitric oxide (NO) reductase, frequently appears in anammox bioreactors depending on the operating conditions. We used genomic and metatranscriptomic analyses to evaluate the metabolic potential ...
Source: MicrobiologyOpen - August 29, 2021 Category: Microbiology Authors: Takashi Okubo,
Hideto Takami Tags: ORIGINAL ARTICLE Source Type: research
Efficiency of the synthetic self ‐splicing RiboJ ribozyme is robust to cis‐ and trans‐changes in genetic background
Genetic insulators, such as RiboJ self-splicing ribozyme, have been used to mitigate unintended context-dependentcis-interactions when designing genetic circuits. Despite the general use of RiboJ as an insulator, there has been no study quantifying the RiboJ splicing efficiency or whether this autocatalytic activity is robust totrans- andcis-genetic context. Here, we show that mRNA molecules containing RiboJ are spliced with a mean efficiency of 98% and neither thecis- nortrans-genetic context has a significant impact on RiboJ activity. AbstractThe expanding knowledge of the variety of synthetic genetic elements has enable...
Source: MicrobiologyOpen - August 24, 2021 Category: Microbiology Authors: Mark éta Vlková,
Bhargava Reddy Morampalli,
Olin K. Silander Tags: ORIGINAL ARTICLE Source Type: research
Marine biofilms on different fouling control coating types reveal differences in microbial community composition and abundance
Marine biofilms constitute the pioneering components of biofouling, a phenomenon with significant environmental and economic impacts in maritime applications. Antifouling control strategies are applied on vessel hulls based on the application of protective paints. Here, we explored the in situ prokaryotic biofilm community composition and abundance on different types of commercial fouling control coatings (fouling release, biocidal) employing next-generation sequencing targeting the 16S rRNA gene. Our results indicated distinct microbial community profiles between coating types, with specific genera contributing to differe...
Source: MicrobiologyOpen - August 24, 2021 Category: Microbiology Authors: Maria Papadatou,
Samuel C. Robson,
Sergey Dobretsov,
Joy E. M. Watts,
Jennifer Longyear,
Maria Salta Tags: ORIGINAL ARTICLE Source Type: research
Non ‐ribosomal insights from the ribosomal P2 protein in Plasmodium falciparum‐infected erythrocytes
In malaria parasites, the 60S ribosomal stalk protein P2 translocates to the infected red blood cell surface and is potentially involved in the regulation of parasite nuclear division. AbstractThe enormous complexity of the eukaryotic ribosome has been a real challenge in unlocking the mechanistic aspects of its amazing molecular function during mRNA translation and many non-canonical activities of ribosomal proteins in eukaryotic cells. While exploring the uncanny nature of ribosomal P proteins in malaria parasitesPlasmodium falciparum, the 60S stalk ribosomal P2 protein has been shown to get exported to the infected eryt...
Source: MicrobiologyOpen - August 23, 2021 Category: Microbiology Authors: Sudipta Das,
Bhaskar Roy,
Saswata Chakrabarty Tags: COMMENTARY Source Type: research
Non ‐ribosomal insights into ribosomal P2 protein in Plasmodium falciparum‐infected erythrocytes
In malaria parasites, the 60S ribosomal stalk protein P2 translocates to the infected red blood cell surface and is potentially involved in the regulation of parasite nuclear division. AbstractThe enormous complexity of the eukaryotic ribosome has been a real challenge in unlocking the mechanistic aspects of its amazing molecular function during mRNA translation and many non-canonical activities of ribosomal proteins in eukaryotic cells. While exploring the uncanny nature of ribosomal P proteins in malaria parasitesPlasmodium falciparum, the 60S stalk ribosomal P2 protein has been shown to get exported to the infected eryt...
Source: MicrobiologyOpen - August 22, 2021 Category: Microbiology Authors: Sudipta Das,
Bhaskar Roy,
Saswata Chakrabarty Tags: COMMENTARY Source Type: research
Nitrite as a causal factor for nitrate ‐dependent anaerobic corrosion of metallic iron induced by Prolixibacter strains
In this study, we isolated fourProlixibacter strains, which belong to the phylumBacteroidetes, and examined their nitrate respiration- and Fe0-corroding activities, together with two previously isolatedProlixibacter strains. Four of the sixProlixibacter strains reduced nitrate under anaerobic conditions, while the other two strains did not. The anaerobic growth of the four nitrate-reducing strains was enhanced by nitrate, which was not observed in the two strains unable to reduce nitrate. When the nitrate-reducing strains were grown anaerobically in the presence of Fe0 or carbon steel, the corrosion of the materials was en...
Source: MicrobiologyOpen - August 17, 2021 Category: Microbiology Authors: Takao Iino,
Nobuaki Shono,
Kimio Ito,
Ryuhei Nakamura,
Kazuo Sueoka,
Shigeaki Harayama,
Moriya Ohkuma Tags: ORIGINAL ARTICLE Source Type: research
Analysis of N ‐glycosylation in fungal l‐amino acid oxidases expressed in the methylotrophic yeast Pichia pastoris
Four fungal L-amino acid oxidases (LAAOs) were expressed inPichia pastoris as N-glycosylated proteins. To understand the effect of glycosylation on fungal LAAOs 9His-hcLAAO4 fromHebeloma cylindrosporum was analyzed and four N-glycans were detected but only glycosylation at asparagine 54 had a major effect on the specific activity. It seems that a more active conformation required this specific glycosylation during biosynthesis. Abstractl-amino acid oxidases (LAAOs) catalyze the oxidative deamination ofl-amino acids to corresponding α-keto acids. Here, we describe the heterologous expression of four fungal LAAOs inPichia p...
Source: MicrobiologyOpen - August 16, 2021 Category: Microbiology Authors: Marc Christian He ß,
Marvin Grollius,
Valentin Duhay,
Simon Koopmeiners,
Svenja Bloess,
Gabriele Fischer von Mollard Tags: ORIGINAL ARTICLE Source Type: research
