Clay ‐associated microbial communities and their relevance for a nuclear waste repository in the Opalinus Clay rock formation
Biocorrosion and microbial mineral transformation can affect the safety of deep geological repositories for high-level nuclear waste storage. We analyzed the composition and evaluated the importance of mineral-associated microbial communities in Opalinus Clay for safety purposes. Rock-attached communities were dominated by phyla capable of corrosive biofilm formation. Based on the abundance of potential iron-reducing bacteria and available electron acceptors, iron reduction appeared to be the most important process. Site-specific mineralogy and geochemistry have selected for subcommunities and specific metabolic functions....
Source: MicrobiologyOpen - July 11, 2023 Category: Microbiology Authors: Julia Mitzscherling,
Steffi Genderjahn,
Anja M. Schleicher,
Alexander Bartholom äus,
Jens Kallmeyer,
Dirk Wagner Tags: ORIGINAL ARTICLE Source Type: research
Morphological and physiological impacts of salinity on colonial strains of the cyanobacteria Microcystis aeruginosa
High salinity affects the morphology and viability of colonial strains of the cyanobacteriaMicrocystis aeruginosa producing different amounts of mucilage. We demonstrate that the collective organization of colonies embedded in an extracellular polymeric substance improves their ability to cope with osmotic shock when compared to unicellular strains. These results suggestMicrocystis survival and a potential proliferation in mesohaline estuaries after transfer from freshwater. AbstractIn the context of global change and enhanced toxic cyanobacterial blooms, cyanobacterial transfer to estuaries is likely to increase in freq...
Source: MicrobiologyOpen - June 29, 2023 Category: Microbiology Authors: Myriam Bormans,
Benjamin Legrand,
Nicolas Waisbord,
Enora Briand Tags: ORIGINAL ARTICLE Source Type: research
An improved method for intracellular DNA (iDNA) recovery from terrestrial environments
The simultaneous extraction of intracellular DNA (iDNA) and extracellular DNA (eDNA) can be improved by using a highly concentrated sodium phosphate buffer with a detergent mix or EDTA. This improved protocol allows for higher recovery of iDNA from various environmental samples, including low-biomass iron-bearing rock samples, while buffers based solely on sodium phosphate are recommended for studies focusing on eDNA. These modifications may contribute to a more accurate characterization of modern and past ecosystems in environmental studies AbstractThe simultaneous extraction of intracellular DNA (iDNA) and extracellula...
Source: MicrobiologyOpen - June 26, 2023 Category: Microbiology Authors: Diego Medina Caro,
Lucas Horstmann,
Lars Ganzert,
Romulo Oses,
Thomas Friedl,
Dirk Wagner Tags: ORIGINAL ARTICLE Source Type: research
Genome comparison reveals that Halobacterium salinarum 63 ‐R2 is the origin of the twin laboratory strains NRC‐1 and R1
The complete genomes of four Halobacterium salinarum strains were compared in detail. Two strains (91-R6T and 63-R2) were isolated in 1934 by Lochhead from cow and buffalo hides. From the results of these comparisons, we conclude that strain 63-R2 is the immediate ancestor of the two, widely used laboratory strains NRC-1 and R1. AbstractThe genome ofHalobacterium strain 63-R2 was recently reported and provides the opportunity to resolve long-standing issues regarding the source of two widely used model strains ofHalobacterium salinarum, NRC-1 and R1. Strain 63-R2 was isolated in 1934 from a salted buffalo hide (epithet “...
Source: MicrobiologyOpen - June 14, 2023 Category: Microbiology Authors: Friedhelm Pfeiffer,
Mike Dyall ‐Smith Tags: ORIGINAL ARTICLE Source Type: research
Aedes albopictus microbiome derives from environmental sources and partitions across distinct host tissues
The processes that govern how environmental microbes assemble across the tissues within mosquitoes remain poorly resolved. We use ecological network analyses to examine how environmental bacteria assemble to form bacteriomes amongAedes albopictus host tissues. Broadly, these data demonstrate that mosquito tissue microbiomes are nested within environmental sources, and form specialized modules based on tissue type. AbstractThe mosquito microbiome consists of a consortium of interacting microorganisms that reside on and within culicid hosts. Mosquitoes acquire most of their microbial diversity from the environment over their...
Source: MicrobiologyOpen - June 13, 2023 Category: Microbiology Authors: Priscilla S. Seabourn,
Danya E. Weber,
Helen Spafford,
Matthew C. I. Medeiros Tags: ORIGINAL ARTICLE Source Type: research
Potential impacts of environmental bacteria on the microbiota of loggerhead (Caretta caretta) and green (Chelonia mydas) sea turtle eggs and their hatching success
This study characterized and compared the bacterial communities from the (i) cloaca of nesting sea turtles (ii) sand within and surrounding the nests; and (iii) hatched and unhatched eggshells from loggerhead (Caretta caretta) and green (Chelonia mydas) turtles. High throughput sequencing of bacterial 16S ribosomal RNA gene V4 region amplicons was performed on samples collected from 27 total nests in Fort Lauderdale and Hillsboro beaches in southeast Florida, United States. Significant differences were identified between hatched and unhatched egg microbiota with the differences caused predominately byPseudomonas spp., foun...
Source: MicrobiologyOpen - June 5, 2023 Category: Microbiology Authors: Colleen M. McMaken,
Derek A. Burkholder,
Rosanna J. Milligan,
Jose V. Lopez Tags: ORIGINAL ARTICLE Source Type: research
Molecular mechanisms of regulation by a β‐alanine‐responsive Lrp‐type transcription factor from Acidianus hospitalis
Molecular mechanisms of regulation by a beta-alanine-responsive Lrp-type transcription factor fromAcidianus hospitalis. AbstractThe leucine-responsive regulatory protein (Lrp) family of transcriptional regulators is widespread among prokaryotes and especially well-represented in archaea. It harbors members with diverse functional mechanisms and physiological roles, often linked to the regulation of amino acid metabolism. BarR is an Lrp-type regulator that is conserved in thermoacidophilic Thermoprotei belonging to the order Sulfolobales and is responsive to the non-proteinogenic amino acid β-alanine. In this work, we unra...
Source: MicrobiologyOpen - May 23, 2023 Category: Microbiology Authors: Amber J. Bernauw,
Vincent Crabbe,
Fraukje Ryssegem,
Ronnie Willaert,
Indra Bervoets,
Eveline Peeters Tags: ORIGINAL ARTICLE Source Type: research
Development of a new multiplex quantitative PCR for the detection of Glaesserella parasuis, Mycoplasma hyorhinis, and Mycoplasma hyosynoviae
The aim of the study was to develop a multiplex PCR for identification ofGlaesserella parasuis and its virulence markervtaA distinguishing between highly virulent and non-virulent strains. Furthermore,Mycoplasma hyorhinis andMycoplasma hyosynoviae can be identified targeting 16S rRNA. The developed multiplex PCR provides a simple one-tube assay, which can be performed rapidly and cost-efficiently allowing an efficient throughput of samples in veterinary diagnostic laboratories. AbstractGlaesserella parasuis,Mycoplasma hyorhinis, andMycoplasma hyosynoviae are important porcine pathogens responsible for polyserositis, polyar...
Source: MicrobiologyOpen - May 16, 2023 Category: Microbiology Authors: Simone Scherrer,
Sarah Schmitt,
Fenja Rademacher,
Peter Kuhnert,
Giovanni Ghielmetti,
Sophie Peterhans,
Roger Stephan Tags: COMMENTARY Source Type: research
Effects of sponge ‐to‐sponge contact on the microbiomes of three spatially competing Caribbean coral reef species
In this study, we characterized the microbiomes of three common Caribbean sponges (Agelas tubulata,Iotrochota birotulata, andXestospongia muta) observed to naturally interact spatially in Key Largo, Florida (USA). For each species, replicate samples were collected from sponges in contact with neighbors at the site of contact (contact) and distant from the site of contact (no contact), and from sponges spatially isolated from neighbors (control). Next-generation amplicon sequencing (V4 region of 16S rRNA) revealed significant differences in microbial community structure and diversity among sponge species, but no significant...
Source: MicrobiologyOpen - April 28, 2023 Category: Microbiology Authors: Shelby E. Gantt,
Patrick M. Erwin Tags: ORIGINAL ARTICLE Source Type: research
Issue Information
(Source: MicrobiologyOpen)
Source: MicrobiologyOpen - April 27, 2023 Category: Microbiology Tags: ISSUE INFORMATION Source Type: research
Responses of Anabaena sp. PCC7120 to lindane: Physiological effects and differential expression of potential lin genes
In this article, the tolerance ofAnabaena sp. to lindane was studied as well as the expression of its potentiallin genes. Our goal was to obtain information to create strategies for lindane bioremediation and also to find the induction of genes in the presence of lindane that can be used in the development of whole-cell biosensors. AbstractLindane ( γ-HCH) is an organochlorine pesticide that causes huge environmental concerns worldwide due to its recalcitrance and toxicity. The use of the cyanobacteriumAnabaena sp. PCC 7120 in aquatic lindane bioremediation has been suggested but information relative to this process is sc...
Source: MicrobiologyOpen - April 27, 2023 Category: Microbiology Authors: Jorge Gu ío,
Maria F. Fillat,
Maria L. Peleato,
Emma Sevilla Tags: ORIGINAL ARTICLE Source Type: research
Genomics of the “tumorigenes” clade of the family Rhizobiaceae and description of Rhizobium rhododendri sp. nov
This study revealed additional genomic and taxonomic diversity of tumorigenic agrobacteria. Tumorigenic strains isolated from rhododendron represent a novel species of the genusRhizobium for which the nameRhizobium rhododendri sp. nov. is proposed. Both species of the “tumorigenes” clade (R. rhododendri andR. tumorigenes), contain multipartite genomes. Synteny and phylogenetic analyses indicated that a large putative chromid ofR. rhododendri resulted from the cointegration of an ancestral megaplasmid and two putative chromids, following its divergence fromR. tumorigenes. AbstractTumorigenic members of the familyRhizobi...
Source: MicrobiologyOpen - April 1, 2023 Category: Microbiology Authors: Nemanja Kuzmanovi ć,
George C. diCenzo,
Boyke Bunk,
Cathrin Spröer,
Anja Frühling,
Meina Neumann‐Schaal,
Jörg Overmann,
Kornelia Smalla Tags: ORIGINAL ARTICLE Source Type: research
High prevalence of blaCTX ‐M‐15 type extended‐spectrum beta‐lactamases in Gambian hooded vultures (Necrosyrtes monachus): A threatened species with substantial human interaction
In this study, we found that hooded vulture has a high prevalence of ESBL-producing bacteria. Based on these data, hooded vultures as top predators may function as a bioindicator for the presence of antibiotic-resistant bacteria (ARB). Furthermore, this study highlights how vultures are exposed to anthropogenic impact, not only posing a threat to the birds but also serving as vectors for further dissemination of ARB in the environment and transmission to humans. AbstractOne hundred fecal samples from hooded vultures in the Gambia (Banjul area) were investigated for the presence of bacteria with extended-spectrum cephalospo...
Source: MicrobiologyOpen - March 24, 2023 Category: Microbiology Authors: Hanna Woksepp,
Fagimba Camara,
Jonas Bonnedahl Tags: ORIGINAL ARTICLE Source Type: research
Biological and synthetic surfactant exposure increases antimicrobial gene occurrence in a freshwater mixed microbial biofilm environment
Biofilms exposed to biological and synthetic surfactants may experience an increased prevalence of antimicrobial resistance genes. Of the nine total classes tested, eight were present in biofilm exposed to a biological surfactant, five were present after exposure to a synthetic surfactant, and four were present in our control. AbstractAquatic habitats are particularly susceptible to chemical pollution, such as antimicrobials, from domestic, agricultural, and industrial sources. This has led to the rapid increase of antimicrobial resistance (AMR) gene prevalence. Alternate approaches to counteract pathogenic bacteria are in...
Source: MicrobiologyOpen - March 18, 2023 Category: Microbiology Authors: Stephanie P. Gill,
William J. Snelling,
James S. G. Dooley,
Nigel G. Ternan,
Ibrahim M. Banat,
Joerg Arnscheidt,
William R. Hunter Tags: ORIGINAL ARTICLE Source Type: research
Temporal disturbance of a model stream ecosystem by high microbial diversity from treated wastewater
Wastewater represents a substantial portion of river microbiomes worldwide, yet its effect on stream microbiomes remains challenging to study. Here, we use a mesocosm setup combined with strain-resolved metagenomics to unravel the impacts of treated wastewater on previously stressed stream water. The disturbance resulted in a significant shift of the microbiome, which did not persist over 10 days, suggesting that previously stressed stream microbiomes are resilient to the introduction of treated wastewater. AbstractMicrobial communities in freshwater streams play an essential role in ecosystem functioning via biogeochemica...
Source: MicrobiologyOpen - March 15, 2023 Category: Microbiology Authors: Tom L. Stach,
Guido Sieber,
Manan Shah,
Sophie A. Simon,
Andr é Soares,
Till L. V. Bornemann,
Julia Plewka,
Julian Künkel,
Christian Becker,
Folker Meyer,
Jens Boenigk,
Alexander J. Probst Tags: ORIGINAL ARTICLE Source Type: research
