Pantoea ananatis carotenoid production confers toxoflavin tolerance and is regulated by Hfq ‐controlled quorum sensing
Carotenoid production confers tolerance to toxoflavin and UV radiation inPantoea ananatis. We proposed a model of carotenoid production for the previously reported regulatory network HfqArcZ→ RpoS Ͱ ClpXP and that identified here, in which Hfq‐controlled quorum signaling derepresses EanR to activate RpoS expression, thereby initiating carotenoid production. AbstractCarotenoids are widely used in functional foods, cosmetics, and health supplements, and their importance and scope of use are continuously expanding. Here, we characterized carotenoid biosynthetic genes of the plant ‐pathogenic bacteriumPantoea ananatis, ...
Source: MicrobiologyOpen - February 15, 2021 Category: Microbiology Authors: Okhee Choi,
Byeongsam Kang,
Yongsang Lee,
Yeyeong Lee,
Jinwoo Kim Tags: ORIGINAL ARTICLE Source Type: research
Epipelagic microbiome of the Small Aral Sea: Metagenomic structure and ecological diversity
This study aimed to describe and analyze the microbial community from one location of the Small Aral Sea (SAS) using metagenomic approaches. In total 78% of the reads could be assigned to domains of bacteria (2,239,543 reads) and archaea (46,904 reads), while the other 22% of the sequences belonged to eukaryotes and viruses. We presume that the observed broad range of phylogenetic and ecological features displayed by the genetic signatures may demonstrate intensive mixing of water masses originating from different ecological niches of the Aral ‐Syr Darya River basin. AbstractMicrobial diversity studies regarding the aqua...
Source: MicrobiologyOpen - February 15, 2021 Category: Microbiology Authors: Madina Alexyuk,
Andrey Bogoyavlenskiy,
Pavel Alexyuk,
Yergali Moldakhanov,
Vladimir Berezin,
Ilya Digel Tags: ORIGINAL ARTICLE Source Type: research
No man's land: Species ‐specific formation of exclusion zones bordering Actinomyces graevenitzii microcolonies in nanoliter cultures
“Exclusion zone” formation aroundActinomyces graevenitzii microcolonies in microfluidic nanoliter volume co ‐culture chambers. This species‐specific phenomenon appears to relate toA. graevenitzii colony stress and can alter inflammatory responses. AbstractTo survive within complex environmental niches, including the human host, bacteria have evolved intricate interspecies communities driven by competition for limited nutrients, cooperation via complementary metabolic proficiencies, and establishment of homeostatic relationships with the host immune system. The study of such complex, interdependent relationships i...
Source: MicrobiologyOpen - February 14, 2021 Category: Microbiology Authors: Fatemeh Jalali,
Felix Ellett,
Pooja Balani,
Margaret J. Duncan,
Floyd E. Dewhirst,
Gary G. Borisy,
Daniel Irimia Tags: ORIGINAL ARTICLE Source Type: research
Seasonal variation of viral infections between the eastern honey bee (Apis cerana) and the western honey bee (Apis mellifera)
This study provides important insights into the complex relationships between viruses and their hosts in different seasons and regions, which will be important for developing effective disease management strategies to improve bee health. (Source: MicrobiologyOpen)
Source: MicrobiologyOpen - February 5, 2021 Category: Microbiology Authors: Gongwen Chen,
Yuqi Wu,
Jie Deng,
Zhengsheng Wen,
Shuai Wang,
Yanping Chen,
Fuliang Hu,
Huoqing Zheng Tags: ORIGINAL ARTICLE Source Type: research
Evaluation of the occurrence of pathogenic free ‐living amoeba and bacteria in 20 public indoor swimming pool facilities
Free ‐living amoebas (FLA) and pathogenic bacteria represent a risk in swimming pools. This work aimed to study the physicochemical characteristics and the occurrence of potentially pathogenic FLA and bacteria in water samples from 20 public indoor swimming facilities in northern Portugal. Our findings highlight the need to establish recommendations to safeguard the health of the swimming pool users. AbstractRecently, indoor swimming pool activities have increased to promote health ‐enhancing physical activities, which require establishing suitable protocols for disinfection and water quality control. Normally, the ass...
Source: MicrobiologyOpen - February 4, 2021 Category: Microbiology Authors: Mar ía Reyes‐Batlle,
Marta F. Gabriel,
Rubén Rodríguez‐Expósito,
Fátima Felgueiras,
Ines Sifaoui,
Zenaida Mourão,
Eduardo Oliveira Fernandes,
José E. Piñero,
Jacob Lorenzo‐Morales Tags: ORIGINAL ARTICLE Source Type: research
Seasonal variation of viral infections between the eastern honey bee (Apis cerana) and the western honey bee (Apis mellifera)
This study provides important insights into the complex relationships between viruses and their hosts in different seasons and regions, which will be important for developing effective disease management strategies to improve bee health. (Source: MicrobiologyOpen)
Source: MicrobiologyOpen - February 4, 2021 Category: Microbiology Authors: Gongwen Chen,
Yuqi Wu,
Jie Deng,
Zhengsheng Wen,
Shuai Wang,
Yanping Chen,
Fuliang Hu,
Huoqing Zheng Tags: ORIGINAL ARTICLE Source Type: research
Spatially explicit depiction of a floral epiphytic bacterial community reveals role for environmental filtering within petals
Spatially explicit analysis of flower petal microbial communities reveals evidence of environmental filtering. AbstractThe microbiome of flowers (anthosphere) is an understudied compartment of the plant microbiome. Within the flower, petals represent a heterogeneous environment for microbes in terms of resources and environmental stress. Yet, little is known of drivers of structure and function of the epiphytic microbial community at the within ‐petal scale. We characterized the petal microbiome in two co‐flowering plants that differ in the pattern of ultraviolet (UV) absorption along their petals. Bacterial communitie...
Source: MicrobiologyOpen - February 4, 2021 Category: Microbiology Authors: Rebecca A. Hayes,
Maria Rebolleda ‐Gómez,
Kristen Butela,
Leah F. Cabo,
Nevin Cullen,
Nancy Kaufmann,
Steffani O'Neill,
Tia‐Lynn Ashman Tags: ORIGINAL ARTICLE Source Type: research
A culture ‐independent approach to understanding the role of soil fungal communities in Bromus tectorum stand failure
Metabarcoding via targeted ITS sequencing was used to characterize fungal communities in cheatgrass soils affected by stand failure in multiple areas. Analysis of the data confirmed key differences in the overall community composition between native communities and those affected by stand failure. Several identified fungal pathogens warrant further investigation to determine whether they are causal agents of stand failure. AbstractCheatgrass (Bromus tectorum L.) is an invasive annual grass (Poaceae) that has colonized large portions of the Intermountain West. Cheatgrass stand failures have been observed throughout the inva...
Source: MicrobiologyOpen - February 4, 2021 Category: Microbiology Authors: Nathan J. Ricks,
Taryn Williamson,
Susan E. Meyer,
John M. Chaston,
Craig E. Coleman Tags: ORIGINAL ARTICLE Source Type: research
Functional and structural characterization of Hyp730, a highly conserved and dormancy ‐specific hypothetical membrane protein
Dormancy requires a bacterium to be tolerant to external stresses, including antibiotics, hypoxia, nutrient deprivation, or immune surveillance from the host organism. Dormant bacteria aim to survive by minimizing their biological events, such as by adopting a state of non ‐replicative persistence, yet the molecular mechanism and protein which regulates dormancy are largely unknown. Here, we identified inMicrococcus luteus a dormancy ‐specific protein, Hyp730 is a membrane protein, widely conserved across Actinobacteria includingMycobacterium tuberculosis, but whose biological function has remained elusive. We determin...
Source: MicrobiologyOpen - February 3, 2021 Category: Microbiology Authors: Stewart Fannin,
Jonathan Rangel,
Abiodun P. Bodurin,
Tannon Yu,
Brandon Mistretta,
Sujina Mali,
Preethi Gunaratne,
Steven J. Bark,
Jerry O. Ebalunode,
Arshad Khan,
William R. Widger,
Mehmet Sen Tags: ORIGINAL ARTICLE Source Type: research
Evaluation of the occurrence of pathogenic free ‐living amoeba and bacteria in 20 public indoor swimming pool facilities
Free ‐living amoebas (FLA) and pathogenic bacteria represent a risk in swimming pools. This work aimed to study the physicochemical characteristics and the occurrence of potentially pathogenic FLA and bacteria in water samples from 20 public indoor swimming facilities in northern Portugal. Our findings highlight the need to establish recommendations to safeguard the health of the swimming pool users. AbstractRecently, indoor swimming pool activities have increased to promote health ‐enhancing physical activities, which require establishing suitable protocols for disinfection and water quality control. Normally, the ass...
Source: MicrobiologyOpen - February 3, 2021 Category: Microbiology Authors: Mar ía Reyes‐Batlle,
Marta F. Gabriel,
Rubén Rodríguez‐Expósito,
Fátima Felgueiras,
Ines Sifaoui,
Zenaida Mourão,
Eduardo Oliveira Fernandes,
José E. Piñero,
Jacob Lorenzo‐Morales Tags: ORIGINAL ARTICLE Source Type: research
Spatially explicit depiction of a floral epiphytic bacterial community reveals role for environmental filtering within petals
Spatially explicit analysis of flower petal microbial communities reveals evidence of environmental filtering. AbstractThe microbiome of flowers (anthosphere) is an understudied compartment of the plant microbiome. Within the flower, petals represent a heterogeneous environment for microbes in terms of resources and environmental stress. Yet, little is known of drivers of structure and function of the epiphytic microbial community at the within ‐petal scale. We characterized the petal microbiome in two co‐flowering plants that differ in the pattern of ultraviolet (UV) absorption along their petals. Bacterial communitie...
Source: MicrobiologyOpen - February 3, 2021 Category: Microbiology Authors: Rebecca A. Hayes,
Maria Rebolleda ‐Gómez,
Kristen Butela,
Leah F. Cabo,
Nevin Cullen,
Nancy Kaufmann,
Steffani O'Neill,
Tia‐Lynn Ashman Tags: ORIGINAL ARTICLE Source Type: research
A culture ‐independent approach to understanding the role of soil fungal communities in Bromus tectorum stand failure
Metabarcoding via targeted ITS sequencing was used to characterize fungal communities in cheatgrass soils affected by stand failure in multiple areas. Analysis of the data confirmed key differences in the overall community composition between native communities and those affected by stand failure. Several identified fungal pathogens warrant further investigation to determine whether they are causal agents of stand failure. AbstractCheatgrass (Bromus tectorum L.) is an invasive annual grass (Poaceae) that has colonized large portions of the Intermountain West. Cheatgrass stand failures have been observed throughout the inva...
Source: MicrobiologyOpen - February 3, 2021 Category: Microbiology Authors: Nathan J. Ricks,
Taryn Williamson,
Susan E. Meyer,
John M. Chaston,
Craig E. Coleman Tags: ORIGINAL ARTICLE Source Type: research
Functional and structural characterization of Hyp730, a highly conserved and dormancy ‐specific hypothetical membrane protein
Dormancy requires a bacterium to be tolerant to external stresses, including antibiotics, hypoxia, nutrient deprivation, or immune surveillance from the host organism. Dormant bacteria aim to survive by minimizing their biological events, such as by adopting a state of non ‐replicative persistence, yet the molecular mechanism and protein which regulates dormancy are largely unknown. Here, we identified inMicrococcus luteus a dormancy ‐specific protein, Hyp730 is a membrane protein, widely conserved across Actinobacteria includingMycobacterium tuberculosis, but whose biological function has remained elusive. We determin...
Source: MicrobiologyOpen - February 3, 2021 Category: Microbiology Authors: Stewart Fannin,
Jonathan Rangel,
Abiodun P. Bodurin,
Tannon Yu,
Brandon Mistretta,
Sujina Mali,
Preethi Gunaratne,
Steven J. Bark,
Jerry O. Ebalunode,
Arshad Khan,
William R. Widger,
Mehmet Sen Tags: ORIGINAL ARTICLE Source Type: research
A Pichia biosensor for high ‐throughput analyses of compounds that can influence mosquito behavior
We have constructed a prototypical whole ‐cell biosensor in Pichia pastoris that can be used to study the molecular interactions of the mosquito olfactory receptor co‐receptor (Orco). The sensor can also be expanded through co‐expression of olfactory receptors to study olfactory cascades and identify molecules that can modulate these receptors. AbstractMosquitoes utilize their sense of smell to locate prey and feed on their blood. Repellents interfere with the biochemical cascades that detect odors. Consequently, repellants are highly effective and resource ‐efficient alternatives for controlling the spread of mosq...
Source: MicrobiologyOpen - February 1, 2021 Category: Microbiology Authors: Julia Nogueira Varela,
Vikramaditya G. Yadav Tags: ORIGINAL ARTICLE Source Type: research
