Plants as a realized niche for Listeria monocytogenes
In this review, we summarize current data providing direct or indirect evidence that plants can serve as habitats forListeria monocytogenes, the causative agent of the food-borne life-threatening disease listeriosis. The mechanisms involved in the complex interplay between plants and this bacterium are addressed, and whether this pathogen elicits an immune response in plants is discussed. The combination of plant intrinsic factors, extrinsic biotic factors, and abiotic environmental factors draws the boundaries of plant habitat colonization byL. monocytogenes. AbstractListeria monocytogenes is a human pathogen. It is the c...
Source: MicrobiologyOpen - December 20, 2021 Category: Microbiology Authors: Hoai ‐Nam Truong,
Dominique Garmyn,
Laurent Gal,
Carine Fournier,
Yann Sevellec,
Sylvain Jeandroz,
Pascal Piveteau Tags: REVIEW Source Type: research
Transcriptomic analysis of Streptococcus agalactiae periprosthetic joint infection
Transcriptomic analysis ofStreptococcus agalactiae periprosthetic joint infection (PJI) was performed using fluid derived from sonication of explanted arthroplasties of subjects withS. agalactiae PJI. Results were compared to those ofS. agalactiae strain NEM316 grownin vitro. Results of functional enrichment analysis are shown; one interesting finding was that genes involved in inorganic ion transport and metabolism were enriched in PJI. AbstractAlthoughStreptococcus agalactiae periprosthetic joint infection (PJI) is not as prevalent as staphylococcal PJI, invasiveS. agalactiae infection is not uncommon. Here, RNA-seq was ...
Source: MicrobiologyOpen - December 17, 2021 Category: Microbiology Authors: Hye ‐Kyung Cho,
Thao Masters,
Kerryl E. Greenwood‐Quaintance,
Stephen Johnson,
Patricio R. Jeraldo,
Nicholas Chia,
Meng Pu,
Matthew P. Abdel,
Robin Patel Tags: ORIGINAL ARTICLE Source Type: research
Multiple classes and isoforms of the RNA polymerase recycling motor protein HelD
Stalled transcription complexes must be removed to prevent harmful collisions with DNA replication and actively transcribing complexes. This work investigates the phylogeny of recently identified HelD proteins that play an important role in removing stalled complexes. Transcription recycling factor HelD is widely distributed across the eubacteria as is represented by three different classes: Class I in the low G+C Gram-positives; Class II in the high G+C Gram-positives; Class III in the Gram-negative deltaproteobacteria. Many organisms contain multiple expressed isoforms of HelD. AbstractEfficient control of transcription ...
Source: MicrobiologyOpen - December 9, 2021 Category: Microbiology Authors: Joachim S. Larsen,
Michael Miller,
Aaron J. Oakley,
Nicholas E. Dixon,
Peter J. Lewis Tags: ORIGINAL ARTICLE Source Type: research
Dynamic interspecies interactions and robustness in a four ‐species model biofilm
Here, we describe the constitution of a model biofilm composed of four bacterial species, highly reproducible and robust against transient perturbations, in which we reveal a network of positive and negative interactions. Furthermore, we identified the molecular determinant of one negative interaction and demonstrated its importance for species distribution and its impact on biofilm robustness by mutational analysis of the multispecies ecosystem. AbstractInterspecific interactions within biofilms determine relative species abundance, growth dynamics, community resilience, and success or failure of invasion by an extraneous...
Source: MicrobiologyOpen - December 8, 2021 Category: Microbiology Authors: Aur élie Baliarda,
Michèle Winkler,
Laurent Tournier,
Colin R. Tinsley,
Stéphane Aymerich Tags: ORIGINAL ARTICLE Source Type: research
Dynamic interspecies interactions and robustness in a four ?species model biofilm
Here, we describe the constitution of a model biofilm composed of four bacterial species, highly reproducible and robust against transient perturbations, in which we reveal a network of positive and negative interactions. Furthermore, we identified the molecular determinant of one negative interaction and demonstrated its importance for species distribution and its impact on biofilm robustness by mutational analysis of the multispecies ecosystem. AbstractInterspecific interactions within biofilms determine relative species abundance, growth dynamics, community resilience, and success or failure of invasion by an extraneous...
Source: MicrobiologyOpen - December 7, 2021 Category: Microbiology Authors: Aur lie Baliarda,
Michle Winkler,
Laurent Tournier,
Colin R. Tinsley,
Stphane Aymerich Tags: ORIGINAL ARTICLE Source Type: research
Magnaporthe oryzae as an expression host for the production of the unspecific peroxygenase AaeUPO from the basidiomycete Agrocybe aegerita
We establishedM. oryzae as a host for the expression of the unspecific peroxygenase from the basidiomycete Agrocybe aegerita (AaeUPO). UPOs are attractive biocatalysts for selective oxyfunctionalization of non-activated carbon-hydrogen bonds. To improve and simplify the isolation of AaeUPO inM. oryzae, we fused aMagnaporthe signal peptide for protein secretion and set it under control of the strong EF1 α-promoter. AbstractThe filamentous fungusMagnaporthe oryzae has the potential to be developed as an alternative platform organism for the heterologous production of industrially important enzymes.M. oryzae is easy to hand...
Source: MicrobiologyOpen - December 3, 2021 Category: Microbiology Authors: Stefan Jacob,
Sebastian Bormann,
Michael Becker,
Luis Antelo,
Dirk Holtmann,
Eckhard Thines Tags: COMMENTARY Source Type: research
Binding of the kringle ‐2 domain of human plasminogen to streptococcal PAM‐type M‐protein causes dissociation of PAM dimers
PAM-type M-proteins on the surface ofStreptococcus pyogenes are monomeric (not the usually depicted dimers) at functional temperatures and bind human plasminogen as monomers. AbstractThe direct binding of human plasminogen (hPg), via its kringle-2 domain (K2hPg), to streptococcal M-protein (PAM), largely contributes to the pathogenesis of Pattern D Group AStreptococcus pyogenes (GAS). However, the mechanism of complex formation is unknown. In a system consisting of a Class II PAM from Pattern D GAS isolate NS88.2 (PAMNS88.2), with one K2hPg binding a-repeat in its A-domain, we employed biophysical techniques to analyze the...
Source: MicrobiologyOpen - December 1, 2021 Category: Microbiology Authors: Olawole Ayinuola,
Yetunde A. Ayinuola,
Cunjia Qiu,
Shaun W. Lee,
Victoria A. Ploplis,
Francis J. Castellino Tags: ORIGINAL ARTICLE Source Type: research
Binding of the kringle ?2 domain of human plasminogen to streptococcal PAM?type M?protein causes dissociation of PAM dimers
PAM-type M-proteins on the surface ofStreptococcus pyogenes are monomeric (not the usually depicted dimers) at functional temperatures and bind human plasminogen as monomers. AbstractThe direct binding of human plasminogen (hPg), via its kringle-2 domain (K2hPg), to streptococcal M-protein (PAM), largely contributes to the pathogenesis of Pattern D Group AStreptococcus pyogenes (GAS). However, the mechanism of complex formation is unknown. In a system consisting of a Class II PAM from Pattern D GAS isolate NS88.2 (PAMNS88.2), with one K2hPg binding a-repeat in its A-domain, we employed biophysical techniques to analyze the...
Source: MicrobiologyOpen - November 30, 2021 Category: Microbiology Authors: Olawole Ayinuola,
Yetunde A. Ayinuola,
Cunjia Qiu,
Shaun W. Lee,
Victoria A. Ploplis,
Francis J. Castellino Tags: ORIGINAL ARTICLE Source Type: research
Insights into the bacterial community composition of farmed Caulerpa lentillifera: A comparison between contrasting health states
This study offers pioneering insights on the co-occurrence ofC. lentillifera-attached bacteria, potential detrimental or beneficial microbes, and a baseline for understanding theC. lentillifera holobiont. Further applied and basic research is urgently needed onC. lentillifera microbiome, shotgun metagenomic, metatranscriptomic, and metabolomic studies as well as bioactivity assays are recommended. (Source: MicrobiologyOpen)
Source: MicrobiologyOpen - November 26, 2021 Category: Microbiology Authors: Germ án A. Kopprio,
Nguyen D. Luyen,
Le Huu Cuong,
Tran Mai Duc,
Anna Fricke,
Andreas Kunzmann,
Le Mai Huong,
Astrid Gärdes Tags: ORIGINAL ARTICLE Source Type: research
Distinct microbiota assembly mechanisms revealed in different reconstruction stages during gut regeneration in the sea cucumber Apostichopus japonicus
Ejection of internal organs was induced in the sea cucumberApostichopus japonicus, and gut immune responses and bacterial diversity during gut regeneration were investigated. We revealed that the reconstruction of the bacterial community involved two stages. The diversity, taxonomic composition, and bacterial interaction of the gut bacterial community and the interaction between the bacterial community and the host were significantly different in the two stages. AbstractApostichopus japonicus is a useful model for studying organ regeneration, and the gut microbiota is important for host organ regeneration. However, the...
Source: MicrobiologyOpen - November 23, 2021 Category: Microbiology Authors: Zichao Yu,
Zhuang Xue,
Chao Liu,
Anguo Zhang,
Qiang Fu,
Kun Yang,
Fang Zhang,
Liyuan Ran Tags: ORIGINAL ARTICLE Source Type: research
The CCAAT ‐binding complex mediates azole susceptibility of Aspergillus fumigatus by suppressing SrbA expression and cleavage
In this study, we found that loss of HapB (a subunit of CBC) causes the upregulation of SrbA and SrbA cleavage-related proteins: RbdB, SppA, and the Dsc complex. This facilitates SrbA cleavage and prolongs the nuclear retention of SrbA to further upregulate expression of Erg11A, suggesting a potential interplay exists between SrbA and CBC. AbstractIn fungal pathogens, the transcription factor SrbA (asterolregulatory element-binding protein, SREBP) and CBC (CCAATbindingcomplex) have been reported to regulate azole resistance by competitively binding the TR34 region (34mer) in the promoter of the drug target gene,erg11A. How...
Source: MicrobiologyOpen - November 23, 2021 Category: Microbiology Authors: Chi Zhang,
Lu Gao,
Yiran Ren,
Huiyu Gu,
Yuanwei Zhang,
Ling Lu Tags: ORIGINAL ARTICLE Source Type: research
The CCAAT ?binding complex mediates azole susceptibility of Aspergillus fumigatus by suppressing SrbA expression and cleavage
In this study, we found that loss of HapB (a subunit of CBC) causes the upregulation of SrbA and SrbA cleavage-related proteins: RbdB, SppA, and the Dsc complex. This facilitates SrbA cleavage and prolongs the nuclear retention of SrbA to further upregulate expression of Erg11A, suggesting a potential interplay exists between SrbA and CBC. AbstractIn fungal pathogens, the transcription factor SrbA (asterolregulatory element-binding protein, SREBP) and CBC (CCAATbindingcomplex) have been reported to regulate azole resistance by competitively binding the TR34 region (34mer) in the promoter of the drug target gene,erg11A. How...
Source: MicrobiologyOpen - November 22, 2021 Category: Microbiology Authors: Chi Zhang,
Lu Gao,
Yiran Ren,
Huiyu Gu,
Yuanwei Zhang,
Ling Lu Tags: ORIGINAL ARTICLE Source Type: research
Detection of antimicrobial resistance genes in urban air
This study aimed to investigate the feasibility of a method for metagenomic analysis of microbial composition and ARGs in the outdoor air. Air samples were collected with a Harvard impactor in the PM10 range at 50 m from a hospital in Budapest. From the DNA yielded from samples of PM10 fraction single-end reads were generated with an Ion Torrent sequencer. During the metagenomic analysis, reads were classified taxonomically. The core bacteriome was defined. Reads were assembled to contigs and the ARG content was analyzed. The dominant genera in the core bacteriome wereBacillus,Acinetobacter,Leclercia andPaenibacillus. Am...
Source: MicrobiologyOpen - November 18, 2021 Category: Microbiology Authors: Ágnes Becsei,
Norbert Solymosi,
István Csabai,
Donát Magyar Tags: COMMENTARY Source Type: research
Development and clinical validation of a droplet digital PCR assay for detecting Acinetobacter baumannii and Klebsiella pneumoniae in patients with suspected bloodstream infections
In conclusion, our study suggests that ddPCR represents a sensitive and rapid method for identifying causal pathogens in blood samples and guiding treatment decisions in the early stages of BSIs. (Source: MicrobiologyOpen)
Source: MicrobiologyOpen - November 16, 2021 Category: Microbiology Authors: Yang Zheng,
Jun Jin,
Ziqiang Shao,
Jingquan Liu,
Run Zhang,
Renhua Sun,
Bangchuan Hu Tags: ORIGINAL ARTICLE Source Type: research
ON ‐rep‐seq as a rapid and cost‐effective alternative to whole‐genome sequencing for species‐level identification and strain‐level discrimination of Listeria monocytogenes contamination in a salmon processing plant
This study is essentially a real industry case from a salmon processing plant. TwentyListeria monocytogenes isolates were analyzed both by ON-rep-seq and WGS to identify and differentiate putativeL.monocytogenes from a routine sampling of processing equipment and products, and finally, compare the strain-level discriminatory power of ON-rep-seq to different analyzing levels delivered from the WGS data. The analyses revealed that among the isolates tested there were three different strains. The isolates of the most frequently detected strain (n = 15) were all detected in the problematic area in the processing plant. The str...
Source: MicrobiologyOpen - November 16, 2021 Category: Microbiology Authors: Gunn Merethe Bj ørge Thomassen,
Lukasz Krych,
Susanne Knøchel,
Lisbeth Mehli Tags: ORIGINAL ARTICLE Source Type: research
