Serratia ‐based toxin cluster elements are associated with a type I fimbria
This article characterizes Sef fimbrial elements that have been observed to be consistently colocated with an ABC toxin complex (Tc) found in pathogenic Serratia species that infect the beetle larvae ofCostelytra giveni. This unique association between the fimbria and Tc has not been documented before in any known Tc system and raises questions about the high selective pressure for maintaining these fimbriae only in bacteria that encode Tcs. Our study also sought to characterize the structure and function of Sef fimbria using bioinformatics, cloning, and experimental approaches. AbstractA soil bacterium in theSerratia genu...
Source: MicrobiologyOpen - January 2, 2024 Category: Microbiology Authors: Lesley Sitter,
Marion Schoof,
Travis R. Glare,
Murray P. Cox,
Peter C. Fineran,
Paul P. Gardner,
Mark R. H. Hurst Tags: ORIGINAL ARTICLE Source Type: research
Issue Information
(Source: MicrobiologyOpen)
Source: MicrobiologyOpen - December 22, 2023 Category: Microbiology Tags: ISSUE INFORMATION Source Type: research
Gut microbiota in the short ‐beaked echidna (Tachyglossus Aculeatus) shows stability across gestation
This study is the first to describe the microbiome composition of short-beaked echidnas across reproductive phases and allows the opportunity for this novel information to be used as a metric of health to aid in the detection of diseases triggered by microbiota dysbiosis. (Source: MicrobiologyOpen)
Source: MicrobiologyOpen - December 15, 2023 Category: Microbiology Authors: Isini Buthgamuwa,
Jane C. Fenelon,
Alice Roser,
Haley Meer,
Stephen D. Johnston,
Ashley M. Dungan Tags: ORIGINAL ARTICLE Source Type: research
Examining the functional space of gut microbiome ‐derived peptides
This article proposes exploring the functional space of gut microbiome-derived peptides using in silico approaches that include machine learning tools, as well as activity-agnostic approaches. AbstractThe human gut microbiome contains thousands of small, novel peptides that could play a role in microbe –microbe and host–microbe interactions, contributing to human health and disease. Although these peptides have not yet been systematically characterized, computational tools can be used to elucidate the bioactivities they may have. This article proposes probing the functional space of gut microb iome-derived peptides (MD...
Source: MicrobiologyOpen - December 12, 2023 Category: Microbiology Authors: Ying ‐Chiang J. Lee Tags: COMMENTARY Source Type: research
Bacillus ‐based probiotic cleansers reduce the formation of dry biofilms on common hospital surfaces
We developed a test method for assessing the prevention of the adhesion of organisms to surfaces that could generate dry biofilm material. The study used a probiotic blend ofBacillus and a formulated product to assess their efficacy and visualize the presence of pathogen analogs on the surface of three material types. AbstractIn the absence of liquid suspension, dry biofilms can form upon hard surfaces within a hospital environment, representing a healthcare-associated infection risk. Probiotic cleansers using generally recognized as safe organisms, such as those of theBacillus genus, represent a potential strategy for t...
Source: MicrobiologyOpen - November 23, 2023 Category: Microbiology Authors: Richard Wormald,
Paul N. Humphreys,
Christopher J. Charles,
Simon P. Rout Tags: COMMENTARY Source Type: research
Correction to Concomitant knockout of target and transporter genes in filamentous fungi by genome coediting
(Source: MicrobiologyOpen)
Source: MicrobiologyOpen - November 9, 2023 Category: Microbiology Tags: CORRIGENDUM Source Type: research
Comparative genomic and metabolomic study of three Streptomyces sp. differing in biological activity
This study illustrates the power of comparative genomics and metabolomics on phylogenetically closely related strains of differing activities to highlight bioactive compounds that could contribute to new sustainable crop protection strategies. (Source: MicrobiologyOpen)
Source: MicrobiologyOpen - October 31, 2023 Category: Microbiology Authors: Alisson Gillon,
Ola Abdelrahman,
Eliane Abou ‐Mansour,
Floriane L'Haridon,
Laurent Falquet,
Pierre‐Marie Allard,
Laure Weisskopf Tags: ORIGINAL ARTICLE Source Type: research
Issue Information
(Source: MicrobiologyOpen)
Source: MicrobiologyOpen - October 28, 2023 Category: Microbiology Tags: ISSUE INFORMATION Source Type: research
Biosynthetic gene cluster synteny: Orthologous polyketide synthases in Hypogymnia physodes, Hypogymnia tubulosa, and Parmelia sulcata
This study focused on biosynthetic gene clusters related to polyketide synthesis. Based on ketosynthase homology, we identified nine highly syntenic clusters present in all three species. Among the four clusters belonging to nonreducing PKSs, two are putatively linked to lichen substances derived from orsellinic acid (orcinol depsides and depsidones, e.g., lecanoric acid, physodic acid, lobaric acid), one to compounds derived from methylated forms of orsellinic acid (beta orcinol depsides, e.g., atranorin), and one to melanins. Five clusters with orthologs in all three species are linked to reducing PKSs. Our study contrib...
Source: MicrobiologyOpen - October 18, 2023 Category: Microbiology Authors: Nadim Ahmad,
Manfred Ritz,
Anjuli Calchera,
J ürgen Otte,
Imke Schmitt,
Thomas Brueck,
Norbert Mehlmer Tags: ORIGINAL ARTICLE Source Type: research
Isolation, characterization, and fibroblast uptake of bacterial extracellular vesicles from Porphyromonas gingivalis strains
In conclusion, bEVs fromP. gingivalis were successfully isolated and characterized, and their uptake into human oral fibroblasts was documented. The bEVs displayed active gingipains demonstrating their origin fromP. gingivalis and the potential role of bEVs in periodontitis. (Source: MicrobiologyOpen)
Source: MicrobiologyOpen - October 17, 2023 Category: Microbiology Authors: Helene R. Haugsten,
Anne K. Kristoffersen,
Trude M. Haug,
Tine M. S øland,
Reidun Øvstebø,
Hans C. D. Aass,
Morten Enersen,
Hilde K. Galtung Tags: ORIGINAL ARTICLE Source Type: research
Transcriptomics ‐guided identification of an algicidal protease of the marine bacterium Kordia algicida OT‐1
In this study, we utilize a transcriptomics-guided approach to identify the secreted serine protease AlpA1 as a key factor in the algicidal process. This discovery offers new approaches for the real-time monitoring and manipulation of algicidal bacteria in algal blooms. AbstractIn recent years, interest in algicidal bacteria has risen due to their ecological importance and their potential as biotic regulators of harmful algal blooms. Algicidal bacteria shape the plankton communities of the oceans by inhibiting or lysing microalgae and by consuming the released nutrients.Kordia algicida strain OT-1 is a model marine algicid...
Source: MicrobiologyOpen - October 11, 2023 Category: Microbiology Authors: Kristy S. Syhapanha,
David A. Russo,
Yun Deng,
Nils Meyer,
Remington X. Poulin,
Georg Pohnert Tags: ORIGINAL ARTICLE Source Type: research
Relationship between the Rod complex and peptidoglycan structure in Escherichia coli
The structures of the peptidoglycan of the wild-type strain, the mutant strain of the Rod complex, and its suppressor mutant strains were observed. The peptidoglycan purified from the Rod complex mutant had many large holes, whereas the number and size of holes were reduced in the suppressor mutants. We visualized the relationship between the Rod complex activity and the peptidoglycan structure. AbstractPeptidoglycan for elongation inEscherichia coli is synthesized by the Rod complex, which includes RodZ. Although various mutant strains of the Rod complex have been isolated, the relationship between the activity of the Rod...
Source: MicrobiologyOpen - October 9, 2023 Category: Microbiology Authors: Risa Ago,
Yuhei O. Tahara,
Honoka Yamaguchi,
Motoya Saito,
Wakana Ito,
Kaito Yamasaki,
Taishi Kasai,
Sho Okamoto,
Taiki Chikada,
Taku Oshima,
Issey Osaka,
Makoto Miyata,
Hironori Niki,
Daisuke Shiomi Tags: ORIGINAL ARTICLE Source Type: research
Dynamic interactions between Candida albicans and different streptococcal species in a multispecies oral biofilm
This study aims to investigate the role of streptococcal species in the development of oral biofilm and the cross-kingdom interactions between some of the members of the commensal MSG and the pathogen yeastC. albicans using a multispecies supragingival biofilm model. A total of nine different in vitro biofilms were grown, quantified with culture analyses, and visually examined with confocal laser scanning microscopy (CLSM). A four-species biofilm without any streptococcal species was used as a basic biofilm. In each subsequent inoculum, one species of MSG was added and afterward combined withStreptococcus mutans. The eight...
Source: MicrobiologyOpen - October 4, 2023 Category: Microbiology Authors: Tenzin Kunchok Lueyar,
Lamprini Karygianni,
Thomas Attin,
Thomas Thurnheer Tags: ORIGINAL ARTICLE Source Type: research
Similarity in milk microbiota in replicates
In this study, DNA from 44 bovine milk samples were extracted twice and the V3 –V4 region of the 16S rRNA gene was sequenced in two separate runs. The FASTQ files from the two data sets were run through the same bioinformatics pipeline using the same settings and results from the two data sets were compared. Milk samples collected maximally 2 h apart were used as replicate s and permitted comparisons to be made within the same run. Results show a significant difference in species richness between the two sequencing runs although Shannon and Simpson's diversity was the same. Multivariate analyses of all samples demonstr...
Source: MicrobiologyOpen - October 1, 2023 Category: Microbiology Authors: Josef Dahlberg,
Erik Pelve,
Johan Dicksved Tags: COMMENTARY Source Type: research
A loophole in soap dispensers mediates contamination with Gram ‐negative bacteria
Liquid soap dispensers are widely used in domestic and clinical settings. In previous studies, the risk of bacterial contamination of refillable systems was pointed out and a bacterial contamination rate of 25%, with values of up to 108 colony-forming units/mL was reported. We contaminated different dispensing systems withPseudomonas aeruginosa /Pluralibacter gergoviae biofilm, to reveal the route of contamination and identified the pressure release of standard pump dispensers as the loophole for microbial contamination. AbstractLiquid soap dispensers are widely used in domestic and clinical settings. In previous studies...
Source: MicrobiologyOpen - September 30, 2023 Category: Microbiology Authors: Ralf Lucassen,
Nicole Leuven,
Dirk Bockm ühl Tags: ORIGINAL ARTICLE Source Type: research
