In vivo and in silico screening for antimicrobial compounds from cyanobacteria
Due to the emerging rise of multi-drug resistant bacteria, the discovery of novel antibiotics is of interest. Through their high chemodiversity of bioactive secondary metabolites, cyanobacteria have proven to be promising microorganisms for the discovery of antimicrobial compounds. This review deals with the screening, including in vivo approaches like bioactivity assays as well as in silico approaches using contemporary genome-mining tools in connection with cyanobacteria. The summarized tools are not only applicable for cyanobacteria and can be transferred to other microorganisms. AbstractDue to the emerging rise of mult...
Source: MicrobiologyOpen - March 16, 2022 Category: Microbiology Authors: Dorina Strieth,
Selina Lenz,
Roland Ulber Tags: REVIEW Source Type: research
Antimicrobial resistance profiles of Escherichia coli and prevalence of extended ‐spectrum beta‐lactamase‐producing Enterobacteriaceae in calves from organic and conventional dairy farms in Switzerland
This study compared antimicrobial resistance (AMR) among commensalEscherichia coli of young calves raised on organic and on conventional dairy farms in Switzerland. Proportions of AMR were higher among organically raised calves and calves with antibiotic usage. Extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae were found exclusively among calves from conventional dairy farms. AbstractThis study compared the antimicrobial resistance (AMR) among commensalEscherichia coli in the fecal microbiota of young calves raised on organic and on conventional dairy farms in Switzerland. Further, fecal carriage of extend...
Source: MicrobiologyOpen - March 16, 2022 Category: Microbiology Authors: Magdalena N üesch‐Inderbinen,
Claudia Hänni,
Katrin Zurfluh,
Sonja Hartnack,
Roger Stephan Tags: ORIGINAL ARTICLE Source Type: research
Development of a novel high resolution melting assay for identification and differentiation of all known 19 serovars of Actinobacillus pleuropneumoniae
In this study, we developed a novel high resolution melting (HRM) assay based on reference strains for each of the 19 known serovars and additional 15 clinicalA. pleuropneumoniae isolates. The novel HRM comprises the species-specific APP-HRM1 and two serovar-specific HRM assays (APP-HRM2 and APP-HRM3). APP-HRM1 allowed polymerase chain reaction (PCR) amplification ofapxIV resulting in anA. pleuropneumoniae specific melting curve, whilenadV specific primers differentiated biovar 2 from biovar 1 isolates. Using APP-HRM2 and APP-HRM3, 13A. pleuropneumoniae serovars can be determined by inspecting the assigned melting temperat...
Source: MicrobiologyOpen - March 16, 2022 Category: Microbiology Authors: Simone Scherrer,
Sophie Peterhans,
Christine Neupert,
Fenja Rademacher,
Giody Bartolomei,
Xaver Sidler,
Roger Stephan Tags: ORIGINAL ARTICLE Source Type: research
Microbial biominers: Sequential bioleaching and biouptake of metals from electronic scraps
The article shows the potential of a microbial sequential process to revalorize electronic scraps and recover metals and rare earth elements harnessing extremotolerant microorganisms. AbstractElectronic scraps (e-scraps) represent an attractive raw material to mine demanded metals, as well as rare earth elements (REEs). A sequential microbial-mediated process developed in two steps was examined to recover multiple elements. First, we made use of an acidophilic bacteria consortium, mainly composed ofAcidiphilium multivorum andLeptospidillum ferriphilum, isolated from acid mine drainages. The consortium was inoculated in a d...
Source: MicrobiologyOpen - February 22, 2022 Category: Microbiology Authors: Camino Garc ía‐Balboa,
Paloma Martínez‐Alesón García,
Victoria López‐Rodas,
Eduardo Costas,
Beatriz Baselga‐Cervera Tags: ORIGINAL ARTICLE Source Type: research
Issue Information
(Source: MicrobiologyOpen)
Source: MicrobiologyOpen - February 9, 2022 Category: Microbiology Tags: ISSUE INFORMATION Source Type: research
Microbial diversity of garden snail mucus
BacteriaAcinetobacter sp. and the Rhizobiaceae family, (Pedobacter sp., Aeromonas sp., Shewanella sp., and Alcaligenes sp.) were identified as main members of the snail mucus-associated bacterial community. AbstractThe search for new natural compounds for application in medicine and cosmetics is a trend in biotechnology. One of the sources of such active compounds is the snail mucus. Snail physiology and the biological activity of their fluids (especially the mucus) are still poorly studied. Only a few previous studies explored the relationship between snails and their microbiome. The present study was focused on the biodi...
Source: MicrobiologyOpen - February 8, 2022 Category: Microbiology Authors: Mihaela Belouhova,
Elmira Daskalova,
Ivaylo Yotinov,
Yana Topalova,
Lyudmila Velkova,
Aleksander Dolashki,
Pavlina Dolashka Tags: COMMENTARY Source Type: research
Protein with negative surface charge distribution, Bnr1, shows characteristics of a DNA ‐mimic protein and may be involved in the adaptation of Burkholderia cenocepacia
Burkholderianegatively surface-chargedregulatory protein 1 (Bnr1) is an acidic protein expressed byBurkholderia cenocepacia that is upregulated in the stationary phase; in response to low oxygen stress; or the hypoxic environment of the chronically infected cystic fibrosis lung. It possesses a negatively charged surface and its deletion dramatically alters the abundance of over a thousand proteins in the cell, with multiple effects on pathogenesis. We show that Bnr1 binds to histone-like binding proteins and behaves as a DNA-mimic protein. AbstractAdaptation of opportunistic pathogens to their host environment requires rep...
Source: MicrobiologyOpen - February 7, 2022 Category: Microbiology Authors: Ruth Dennehy,
Niamh Duggan,
Simon Dignam,
Sarah McCormack,
Eugene Dillon,
Jessica Molony,
Maria Romano,
Yueran Hou,
Laura Ardill,
Matthew V. X. Whelan,
Zuzanna Drulis ‐Kawa,
Tadhg Ó'Cróinín,
Miguel A. Valvano,
Rita Berisio,
Siobhán McC Tags: ORIGINAL ARTICLE Source Type: research
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(Source: MicrobiologyOpen)
Source: MicrobiologyOpen - February 6, 2022 Category: Microbiology Tags: COVER Source Type: research
Living in a bottle: Bacteria from sediment ‐associated Mediterranean waste and potential growth on polyethylene terephthalate
Inner sediments from marine waste were analyzed using scanning electron microscopy, next generation sequencing, and culturomics.Proteobacteria, Bacteroidota, andActinobacteria were predominant in all the samples. Enhanced growth on plastic by several microbial species was observed. AbstractOcean pollution is a worldwide environmental challenge that could be partially tackled through microbial applications. To shed light on the diversity and applications of the bacterial communities that inhabit the sediments trapped in artificial containers, we analyzed residues (polyethylene terephthalate [PET] bottles and aluminum cans) ...
Source: MicrobiologyOpen - January 31, 2022 Category: Microbiology Authors: Àngela Vidal‐Verdú,
Adriel Latorre‐Pérez,
Esther Molina‐Menor,
Joaquin Baixeras,
Juli Peretó,
Manuel Porcar Tags: ORIGINAL ARTICLE Source Type: research
Prevalence of insertion sequence elements in plasmids relating to mgrB gene disruption causing colistin resistance in Klebsiella pneumoniae
Klebsiella pneumoniae is the dominant species carrying colistin-resistance inducing insertion sequence elements ISKpn25, ISKpn26, ISKpn14, and IS903B on plasmids. ISKpn25, ISKpn26, ISKpn14 bearing plasmids have a high carriage of carbapenemase genes and are associated with clinical source isolation relative to IS903B. Clinically derived plasmids that harbor both carbapenemases and IS elements may engender an extensively drug-resistant phenotype during colistin therapy in hospitals, limiting already narrow treatment options. AbstractColistin is a last resort antibiotic for the treatment of carbapenemase producingKlebsiella ...
Source: MicrobiologyOpen - January 28, 2022 Category: Microbiology Authors: Stephen Mark Edward Fordham,
Anna Mantzouratou,
Elizabeth Sheridan Tags: ORIGINAL ARTICLE Source Type: research
Trophic preferences of the pathogen Ralstonia solanacearum and consequences on its growth in xylem sap
This study aimed to explore in-depth the xylem environment where the pathogen is abundant, and its trophic preferences. First, we determined the composition of tomato xylem sap, where fast multiplication of the pathogen occurs. Then, kinetic growth on single and mixtures of carbon sources in relation to this environment was performed to fully quantify growth. Finally, we calculated the concentration of available metabolites in the xylem sap flux to assess how much it can support bacterial growthin planta. Overall, the study underlines the adaptation ofR. solanacearum to the xylem environment and the fact that the pathoge...
Source: MicrobiologyOpen - January 20, 2022 Category: Microbiology Authors: Caroline Baroukh,
Meriem Zemouri,
St éphane Genin Tags: ORIGINAL ARTICLE Source Type: research
Shining light on multi ‐drug resistant Candida auris: Ultraviolet‐C disinfection, wavelength sensitivity, and prevention of biofilm formation of an emerging yeast pathogen
The objectives of this study were (1) To investigate UVC disinfection performances and wavelength sensitivity ofC. auris. (2) To evaluate the UVC dose required for the prevention of biofilm formation on stainless-steel, plastic (polystyrene), and poly-cotton fabric surfaces.C. auris was grown following standard procedures. The study utilized six different UVC LED arrays with wavelengths between 252 and 280 nm. Arrays were set at similar intensities, to obtain doses of 5–40 mJ cm−2 and similar irradiation time. Disinfection performance for each array was determined using log reduction value (LRV) and percentage r...
Source: MicrobiologyOpen - January 19, 2022 Category: Microbiology Authors: Richard M. Mariita,
James H. Davis,
Michelle M. Lottridge,
Rajul V. Randive Tags: ORIGINAL ARTICLE Source Type: research
Efficacy of antifungal agents against fungal spores: An in vitro study using microplate laser nephelometry and an artificially infected 3D skin model
This study aimed to investigate the sporicidal effects of antimycotics using microplate laser nephelometry and infected three-dimensional full skin models. Sertaconazole nitrate inhibited the growth of dermatophytes, molds, and yeasts. Ciclopirox olamine also had antifungal activity, although, higher concentrations were needed compared to sertaconazole nitrate. Terbinafine was highly effective against most dermatophytes, but higher concentrations were required to kill the resistant strain ofTrichophyton indotineae. AbstractDermal fungal infections seem to have increased over recent years. There is further a shift from anth...
Source: MicrobiologyOpen - January 15, 2022 Category: Microbiology Authors: Sarah Fink,
Anke Burmester,
Uta ‐Christina Hipler,
Claudia Neumeister,
Marcus R. Götz,
Cornelia Wiegand Tags: ORIGINAL ARTICLE Source Type: research
Impact of antibiotics on the human microbiome and consequences for host health
Antibiotics can have several negative impacts on host health; both direct and indirect effects. The gut microbiota plays a crucial role in host health regulation. Effects of antibiotics on the host through the gut microbiome are immense and can affect various functions including immune regulation, metabolic activities, and thus overall health. AbstractIt is well established that the gut microbiota plays an important role in host health and is perturbed by several factors including antibiotics. Antibiotic-induced changes in microbial composition can have a negative impact on host health including reduced microbial diversity...
Source: MicrobiologyOpen - January 13, 2022 Category: Microbiology Authors: Dhrati V. Patangia,
Cornelius Anthony Ryan,
Eugene Dempsey,
Reynolds Paul Ross,
Catherine Stanton Tags: REVIEW Source Type: research
Novel nitrite reductase domain structure suggests a chimeric denitrification repertoire in the phylum Chloroflexi
This study identifies a novel Nir domain architecture and expanded diversity in a rarely reported nitric oxide reductase variant (eNOR) in members of the bacterial phylum Chloroflexi. AbstractDenitrification plays a central role in the global nitrogen cycle, reducing and removing nitrogen from marine and terrestrial ecosystems. The flux of nitrogen species through this pathway has a widespread impact, affecting ecological carrying capacity, agriculture, and climate. Nitrite reductase (Nir) and nitric oxide reductase (NOR) are the two central enzymes in this pathway. Here we present a previously unreported Nir domain archit...
Source: MicrobiologyOpen - January 13, 2022 Category: Microbiology Authors: Sarah L. Schwartz,
Lily Momper,
Luiz Thiberio Rangel,
Cara Magnabosco,
Jan P. Amend,
Gregory P. Fournier Tags: ORIGINAL ARTICLE Source Type: research
