Metabolic potential of the imperfect denitrifier Candidatus Desulfobacillus denitrificans in an anammox bioreactor

The imperfect denitrifierCa. D. denitrificans inhabiting anammox bioreactors can use a range of electron donors for denitrification, including aromatic compounds, glucose, sulfur compounds, and hydrogen. However, our metatranscriptomic analysis suggested that the major electron donors are aromatic compounds, which inhibit anammox activity. AbstractThe imperfect denitrifier,Candidatus (Ca.) Desulfobacillus denitrificans, which lacks nitric oxide (NO) reductase, frequently appears in anammox bioreactors depending on the operating conditions. We used genomic and metatranscriptomic analyses to evaluate the metabolic potential ofCa. D. denitrificans and deduce its functional relationships to anammox bacteria (i.e.,Ca. Brocadia pituitae). AlthoughCa. D. denitrificans is hypothesized to supply NO toCa. B. pituitae as a byproduct of imperfect denitrification, this microbe also possesses hydroxylamine oxidoreductase, which catalyzes the oxidation of hydroxylamine to NO and potentially the reverse reaction.Ca. D. denitrificans can use a range of electron donors for denitrification, including aromatic compounds, glucose, sulfur compounds, and hydrogen, but metatranscriptomic analysis suggested that the major electron donors are aromatic compounds, which inhibit anammox activity. The interrelationship betweenCa. D. denitirificans andCa. B. pituitae via the metabolism of aromatic compounds may govern the population balance of both species.Ca. D. denitrificans also has the potential to fix...

Source: MicrobiologyOpen - August 29, 2021 Category: Microbiology Authors: Takashi Okubo, Hideto Takami Tags: ORIGINAL ARTICLE Source Type: research