Nuclear conditions of basidiospores and hyphal cells in the edible mushroom Oudemansiella aparlosarca

The life cycle of the fungusOudemansiella aparlosarca includes homokaryotic and heterokaryotic basidiospores with four nuclear conditions: non-nucleate, mononucleate, binucleate, and multinucleate. The number of binucleate cells was high in heterokaryotic hyphal cells, while that of multinucleated cells was high in homokaryotic hyphal cells. The predominance of binucleate spores might be a result of post-meiotic mitosis. AbstractOudemansiella aparlosarca is an edible mushroom possessing medicinal and health benefits. Although there are studies on the cultivation ofO.aparlosarca, only a few studies have focused on its genetics and life cycle. Therefore, the main objective of this study was to identify the nuclear conditions of basidiospores and homokaryotic and heterokaryotic hyphal cells and to determine the influence of different nuclear conditions on basidiospore diameter inO.aparlosarca. Two parental strains: strain-55 and strain-81 were used. Staining of basidiospores and hyphal cells in the apical region was performed. We observed the following nuclear conditions: non-nucleate, mononucleate, binucleate, and multinucleate. In both parental strains, binucleate spores were predominant, while the number of non-nucleate spores was the lowest. The diameter of non-nucleate spores was the smallest, being 11.52  µm and 12.15 µm in parental strain-81 and strain-55, respectively, while multinucleate spores had the largest diameter, being 14.78 µm in both parental strains. Bot...
Source: MicrobiologyOpen - Category: Microbiology Authors: Tags: ORIGINAL ARTICLE Source Type: research