Journal of Mammary Gland Biology and Neoplasia This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

GATA3 Truncating Mutations Promote Cistromic Re-Programming In Vitro, but Not Mammary Tumor Formation in Mice
AbstractHeterozygous mutations in the transcription factor GATA3 are identified in 10 –15% of all breast cancer cases. Most of these are protein-truncating mutations, concentrated within or downstream of the second GATA-type zinc-finger domain. Here, we investigated the functional consequences of expression of two truncated GATA3 mutants, in vitro in breast cancer cell lines and in vivo in the mouse mammary gland. We found that the truncated GATA3 mutants display altered DNA binding activity caused by preferred tethering through FOXA1. In addition, expression of the truncated GATA3 mutants reduces E-cadherin expression a...
Source: Journal of Mammary Gland Biology and Neoplasia - June 18, 2019 Category: Cancer & Oncology Source Type: research

BRCA1 Attenuates Progesterone Effects on Proliferation and NF κB Activation in Normal Human Mammary Epithelial Cells
This study investigates the effect of reduced BRCA1 expression on proliferation and NFκB activation in human breast cells, and the impact of progesterone on these effects. The major findings are that: 1) Reduced BRCA1 levels inhibit cell growth in normal human mammary cells and breast cancer cells; 2) Reduced BRCA1 levels stimulated i nflammatory targets and NFκB activity in normal human mammary cells; 3) Wildtype BRCA1 inhibited the pro-proliferative effects of progesterone in normal mammary epithelial cells, and; 4) Progesterone attenuated BRCA1-mediated NFκB activation in normal human mammary cells. These data have i...
Source: Journal of Mammary Gland Biology and Neoplasia - May 17, 2019 Category: Cancer & Oncology Source Type: research

Emerging Role of SOX Proteins in Breast Cancer Development and Maintenance
AbstractTheSOX genes encode a family of more than 20 transcription factors that are critical regulators of embryogenesis and developmental processes and, when aberrantly expressed, have been shown to contribute to tumor development and progression in both an oncogenic and tumor suppressive role. Increasing evidence demonstrates that the SOX proteins play essential roles in multiple cellular processes that mediate or contribute to oncogenic transformation and tumor progression. In the context of breast cancer, SOX proteins function both as oncogenes and tumor suppressors and have been shown to be associated with tumor stage...
Source: Journal of Mammary Gland Biology and Neoplasia - May 8, 2019 Category: Cancer & Oncology Source Type: research

Metformin and Breast Cancer: Molecular Targets
AbstractMetformin has been the first-line drug for the treatment of type II diabetes mellitus for decades, being presently the most widely prescribed antihyperglycemic drug. Retrospective studies associate the use of metformin with a reduction in cancer incidence and cancer-related death. However, despite extensive research about the molecular effects of metformin in cancer cells, its mode of action remains controversial. The major molecular targets of metformin include complex I of the mitochondrial electron transport chain, adenosine monophosphate (AMP)-activated protein kinase (AMPK), and mechanistic target of rapamycin...
Source: Journal of Mammary Gland Biology and Neoplasia - March 21, 2019 Category: Cancer & Oncology Source Type: research

A Syngeneic ErbB2 Mammary Cancer Model for Preclinical Immunotherapy Trials
AbstractIn order to develop a practical model of breast cancer, with in vitro and syngeneic,  immune-intact, in vivo growth capacity, we established a primary cell line derived from a mammary carcinoma in the transgenic FVB/N-Tg(MMTV-ErbB2*)NDL2-5Mul mouse, referred to as “NDLUCD”. The cell line is adapted to standard cell culture and can be transplanted into syngeneic FVB/N mice. The line maintains a stable phenotype over multiple in vitro passages and rounds of in vivo transplantation. NDLUCD tumors in FVB/N mice exhibit high expression ofErbB2 andErbB3 and signaling molecules downstream ofErbB2. The syngeneic tran...
Source: Journal of Mammary Gland Biology and Neoplasia - February 27, 2019 Category: Cancer & Oncology Source Type: research

Inhibition of Tumor Progression by N G -Nitro-L-arginine Methyl Ester in 7,12- dimethylbenz(a)anthracene Induced Breast Cancer: Nitric Oxide Synthase Inhibition as an Antitumor Prevention
AbstractBreast cancer has high mortality rates among the women in the Republic of Armenia. It is now evident that nitric oxide plays important roles in various stages of carcinogenesis such as oncogene activation, tumor suppressor genes, modulation of apoptosis and metastasis. Advances in our understanding of the metabolism and molecular functions of arginine alterations in cancer have led to resurgence in the interest of targeting arginine catabolism, as an anticancer strategy. NOS inhibitors have been proposed, as a way to treat cancer. We have been researching the anti-tumor potential of the NOS inhibition by NG-Nitro-L...
Source: Journal of Mammary Gland Biology and Neoplasia - February 26, 2019 Category: Cancer & Oncology Source Type: research

RETRACTED ARTICLE: Inhibition of Tumor Progression by N G -Nitro-L-arginine Methyl Ester in 7,12- dimethylbenz(a)anthracene Induced Breast Cancer: Nitric Oxide Synthase Inhibition as an Antitumor Prevention
(Source: Journal of Mammary Gland Biology and Neoplasia)
Source: Journal of Mammary Gland Biology and Neoplasia - February 25, 2019 Category: Cancer & Oncology Source Type: research

Detection of Milk Ejection Using Bioimpedance Spectroscopy in Lactating Women during Milk Expression Using an Electric Breast Pump
AbstractMilk ejection is essential for effective milk removal during breastfeeding and pumping, and for continued milk synthesis. Many women are unable to accurately sense milk ejection to determine whether their infant is receiving milk or, when pumping, to switch the pump to a more effective expression pattern. To determine if changes in bioimpedance parameters are associated with milk ejection in the lactating breast during pumping. 30 lactating women participated in 2 pumping sessions within 2  weeks of each other. During pumping the breasts were monitored with bioimpedance spectroscopy (on either the pumped or the no...
Source: Journal of Mammary Gland Biology and Neoplasia - February 13, 2019 Category: Cancer & Oncology Source Type: research

Mammary Epithelial Cell Lineage Changes During Cow ’s Life
AbstractMilk production is highly dependent on the optimal development of the mammary epithelium. It is therefore essential to better understand mammary epithelial cell growth and maintenance from the related epithelial lineage during the animal life. Here, we characterized the epithelial lineage at puberty, lactation and dry-off in bovine using the cell surface markers CD49f, CD24, and CD10. The pubertal period was characterized by a high proportion of CD49fpos cells corresponding to various epithelial subpopulations, notably the CD24pos subpopulations. The proportion of CD49fpos cells was weaker during lactation and dry-...
Source: Journal of Mammary Gland Biology and Neoplasia - February 13, 2019 Category: Cancer & Oncology Source Type: research

Application of the D492 Cell Lines to Explore Breast Morphogenesis, EMT and Cancer Progression in 3D Culture
AbstractThe human female breast gland is composed of branching epithelial ducts that extend from the nipple towards the terminal duct lobular units (TDLUs), which are the functional, milk-producing units of the gland and the site of origin of most breast cancers. The epithelium of ducts and TDLUs is composed of an inner layer of polarized luminal epithelial cells and an outer layer of contractile myoepithelial cells, separated from the vascular-rich stroma by a basement membrane. The luminal- and myoepithelial cells share an origin and in recent years, there has been increasing understanding of how these cell types interac...
Source: Journal of Mammary Gland Biology and Neoplasia - January 25, 2019 Category: Cancer & Oncology Source Type: research

A Longitudinal Study of the Association between Mammographic Density and Gene Expression in Normal Breast Tissue
AbstractHigh mammographic density (MD) is associated with a 4 –6 times increase in breast cancer risk. For post-menopausal women, MD often decreases over time, but little is known about the underlying biological mechanisms. MD reflects breast tissue composition, and may be associated with microenvironment subtypes previously identified in tumor-adjacent norm al tissue. Currently, these subtypes have not been explored in normal breast tissue. We obtained biopsies from breasts of healthy women at two different time points several years apart and performed microarray gene expression analysis. At time point 1, 65 samples wit...
Source: Journal of Mammary Gland Biology and Neoplasia - January 6, 2019 Category: Cancer & Oncology Source Type: research

A Comparative Review of Mixed Mammary Tumors in Mammals
AbstractMixed tumors are characterized by the histological identification of two or more cell types. Commonly, a mixture of epithelial and myoepithelial cells is included in abundant stroma, which can consist of myxoid, chondroid or bony matrices. Spontaneously arising mixed tumors are rare lesions in the human breast but are common in human salivary glands and canine mammary glands. Subtle histopathological characteristics and overlapping attributes of malignant lesions with other benign lesions can lead to a diagnostic challenge. Mixed tumors can present as benign or malignant. While malignant mixed tumors are quite rare...
Source: Journal of Mammary Gland Biology and Neoplasia - November 28, 2018 Category: Cancer & Oncology Source Type: research

A Novel 3-Dimensional Co-culture Method Reveals a Partial Mesenchymal to Epithelial Transition in Breast Cancer Cells Induced by Adipocytes
AbstractCancer metastases are accountable for almost 90% of all human cancer related deaths including from breast cancer (BC). Adipocytes can alter the tumor microenvironment, which can promote metastasis by inducing an epithelial-to-mesenchymal transition (EMT) in BC cells. However, the role of adipocytes during the mesenchymal-to-epithelial transition (MET), that can be important in metastasis, is not clear. To understand the effect of adipocytes on the BC progression, there is a requirement for a better in vitro 3-dimensional (3D) co-culture system that mimics the breast tissue and allows for more accurate analysis of E...
Source: Journal of Mammary Gland Biology and Neoplasia - November 24, 2018 Category: Cancer & Oncology Source Type: research

Correction to: The Emerging Roles of Steroid Hormone Receptors in Ductal Carcinoma in Situ (DCIS) of the Breast
The original version of this article unfortunately contained mistakes. (Source: Journal of Mammary Gland Biology and Neoplasia)
Source: Journal of Mammary Gland Biology and Neoplasia - November 21, 2018 Category: Cancer & Oncology Source Type: research

Human Ductal Carcinoma In Situ: from the Eyes of a Beholder
(Source: Journal of Mammary Gland Biology and Neoplasia)
Source: Journal of Mammary Gland Biology and Neoplasia - November 8, 2018 Category: Cancer & Oncology Source Type: research