A molecular toolbox to study progesterone receptor signaling
AbstractProgesterone receptor (PR) signaling is required for mammary gland development and homeostasis. A major bottleneck in studying PR signaling is the lack of sensitive assays to measure and visualize PR pathway activity both quantitatively and spatially. Here, we develop new tools to study PR signaling in human breast epithelial cells. First, we generate optimized Progesterone Responsive Element (PRE)-luciferase constructs and demonstrate that these new reporters are a powerful tool to quantify PR signaling activity across a wide range of progesterone concentrations in two luminal breast cancer cell lines, MCF7 and T4...
Source: Journal of Mammary Gland Biology and Neoplasia - November 29, 2023 Category: Cancer & Oncology Source Type: research

High-fat diet, but not duration of lactation, increases mammary gland lymphatic vessel function and subsequent growth of inflammatory breast cancer cells
AbstractInflammatory breast cancer (IBC) presents as rapid-onset swelling and breast skin changes caused by tumor emboli in the breast and breast skin lymphatics. IBC has been linked with obesity and duration of breastfeeding, but how these factors affect IBC tumor progression is not clear. We modeled the simultaneous effects of diet and weaning in mice on in vivo lymphatic function; on IBC tumor growth; and on aspects of the mammary gland microenvironment before and after IBC (SUM149) xenograft inoculation. We hypothesized that weaning status and diet would have synergistic effects on lymphatic function and the breast mic...
Source: Journal of Mammary Gland Biology and Neoplasia - October 6, 2023 Category: Cancer & Oncology Source Type: research

Fourteenth Annual ENBDC Workshop: Methods in Mammary Gland Biology and Breast Cancer
AbstractThe fourteenth annual workshop of the European Network for Breast Development and Cancer (ENBDC) onMethods in Mammary Gland Biology and Breast Cancer was held on April 26th − 29th in Weggis, Switzerland. For the first time, early career researchers organised and took part in an additional ECR workshop on the 26th of April, which was received with great enthusiasm. The topics of the main workshop included mammary branching and morphogenesis, novel experimental syste ms (model organisms), systemic influences on tumour progression and the tumour microenvironment. Novel and recent findings were shared across excell...
Source: Journal of Mammary Gland Biology and Neoplasia - October 6, 2023 Category: Cancer & Oncology Source Type: research

High-fat diet, but not duration of lactation, increases mammary gland lymphatic vessel function and subsequent growth of inflammatory breast cancer cells
AbstractInflammatory breast cancer (IBC) presents as rapid-onset swelling and breast skin changes caused by tumor emboli in the breast and breast skin lymphatics. IBC has been linked with obesity and duration of breastfeeding, but how these factors affect IBC tumor progression is not clear. We modeled the simultaneous effects of diet and weaning in mice on in vivo lymphatic function; on IBC tumor growth; and on aspects of the mammary gland microenvironment before and after IBC (SUM149) xenograft inoculation. We hypothesized that weaning status and diet would have synergistic effects on lymphatic function and the breast mic...
Source: Journal of Mammary Gland Biology and Neoplasia - October 6, 2023 Category: Cancer & Oncology Source Type: research

Fourteenth Annual ENBDC Workshop: Methods in Mammary Gland Biology and Breast Cancer
AbstractThe fourteenth annual workshop of the European Network for Breast Development and Cancer (ENBDC) onMethods in Mammary Gland Biology and Breast Cancer was held on April 26th − 29th in Weggis, Switzerland. For the first time, early career researchers organised and took part in an additional ECR workshop on the 26th of April, which was received with great enthusiasm. The topics of the main workshop included mammary branching and morphogenesis, novel experimental syste ms (model organisms), systemic influences on tumour progression and the tumour microenvironment. Novel and recent findings were shared across excell...
Source: Journal of Mammary Gland Biology and Neoplasia - October 6, 2023 Category: Cancer & Oncology Source Type: research

RETRACTED ARTICLE: Pure Primary Squamous Cell Carcinoma of the Breast Arising from the Epithelium of a Complex Mammary Cyst: A Case Report
(Source: Journal of Mammary Gland Biology and Neoplasia)
Source: Journal of Mammary Gland Biology and Neoplasia - August 1, 2023 Category: Cancer & Oncology Source Type: research

Non-Linear Lymphatic Anatomy in Breast Cancer Patients Prior to Axillary Lymph Node Dissection: A Risk Factor For Lymphedema Development
This study aims to determine potential inciting factors that help explain why some patients have non-linear patterns, and what these patterns represent regarding the relative risk of developing postoperative breast cancer related lymphedema in this population. A retrospective review was conducted to identify breast cancer patients who underwent successful ILR with preoperative ICG at our institution from November 2017 —June 2022. Among the 248 patients who were identified, 13 (5%) had preoperative non-linear lymphatic anatomy. A history of trauma or surgery of the affected limb and an increasing number of sentinel lymph ...
Source: Journal of Mammary Gland Biology and Neoplasia - July 22, 2023 Category: Cancer & Oncology Source Type: research

Distinct Requirements for Adaptor Proteins NCK1 and NCK2 in Mammary Gland Development
AbstractThe adaptor proteins NCK1 and NCK2 are well-established signalling nodes that regulate diverse biological processes including cell proliferation and actin dynamics in many tissue types. Here we have investigated the distribution and function ofNck1 andNck2 in the developing mouse mammary gland. Using publicly available single-cell RNA sequencing data, we uncovered distinct expression profiles between the two paralogs.Nck1 showed widespread expression in luminal, basal, stromal and endothelial cells, whileNck2 was restricted to luminal and basal cells, with prominent enrichment in hormone-sensing luminal subtypes. N...
Source: Journal of Mammary Gland Biology and Neoplasia - July 21, 2023 Category: Cancer & Oncology Source Type: research

Imagine beyond: recent breakthroughs and next challenges in mammary gland biology and breast cancer research
AbstractOn 8 December 2022 the organizing committee of the European Network for Breast Development and Cancer labs (ENBDC) held its fifth annual Think Tank meeting in Amsterdam, the Netherlands. Here, we embraced the opportunity to look back to identify the most prominent breakthroughs of the past ten years and to reflect on the main challenges that lie ahead for our field in the years to come. The outcomes of these discussions are presented in this position paper, in the hope that it will serve as a summary of the current state of affairs in mammary gland biology and breast cancer research for early career researchers and...
Source: Journal of Mammary Gland Biology and Neoplasia - July 14, 2023 Category: Cancer & Oncology Source Type: research

Gestational Breast Cancer – a Review of Outcomes, Pathophysiology, and Model Systems
AbstractThe onset of pregnancy marks the start of offspring development, and represents the key physiological event that induces re-organization and specialization of breast tissue. Such drastic tissue remodeling has also been linked to epithelial cell transformation and the establishment of breast cancer (BC). While patient outcomes for BC overall continue to improve across subtypes, prognosis remains dismal for patients with gestational breast cancer (GBC) and post-partum breast cancer (PPBC), as pregnancy and lactation pose additional complications and barriers to several gold standard clinical approaches. Moreover, del...
Source: Journal of Mammary Gland Biology and Neoplasia - July 14, 2023 Category: Cancer & Oncology Source Type: research

Studying Mammary Physiology and Pathology in Domestic Species Benefits Both Humans and Animals
(Source: Journal of Mammary Gland Biology and Neoplasia)
Source: Journal of Mammary Gland Biology and Neoplasia - July 14, 2023 Category: Cancer & Oncology Source Type: research