Performance of Softcup ® menstrual cup and vulvovaginal swab samples for detection and quantification of genital cytokines
J Immunol Methods. 2024 Mar 5;528:113656. doi: 10.1016/j.jim.2024.113656. Online ahead of print.ABSTRACTCytokines are important mediators of immunity in the female genital tract, and their levels may be associated with various reproductive health outcomes. However, the measurement of cytokines and chemokines in vaginal fluid samples may be influenced by a variety of factors, each with the potential to affect the sensitivity and accuracy of the assay, including the interpretation and comparison of data. We measured and compared cytokine milieu in samples collected via Softcup® menstrual cup versus vulvovaginal swabs. One h...
Source: Journal of Immunological Methods - March 6, 2024 Category: Allergy & Immunology Authors: Nashlin Pillay Gugulethu Favourate Mzobe Marothi Letsoalo Asavela Olona Kama Andile Mtshali Stanley Nzuzo Magini Nikkishia Singh Vani Govender Natasha Samsunder Megeshinee Naidoo Dhayendre Moodley Cheryl Baxter Derseree Archary Sinaye Ngcapu Source Type: research
Development and basic performance verification of a rapid homogeneous bioassay for agonistic antibodies against the thyroid-stimulating hormone receptor
J Immunol Methods. 2024 Mar 4:113655. doi: 10.1016/j.jim.2024.113655. Online ahead of print.ABSTRACTGraves' disease is a type of autoimmune hyperthyroidism caused by thyroid-stimulating antibodies (TSAb).1 The combination of a porcine thyroid cell bioassay and cyclic adenosine monophosphate (cAMP) immunoassay (TSAb-enzyme immunoassay; EIA) is a clinically approved TSAb measurement method. Due to the requirement of multiple procedures and a long assay time of 6 h in the TSAb-EIA, a simplified and rapid assay is desired. Herein, we developed a rapid homogeneous TSAb bioassay (rapid-TSAb assay) using the human embryonic kidne...
Source: Journal of Immunological Methods - March 6, 2024 Category: Allergy & Immunology Authors: Motoki Hoshina Shiomi Ojima Atsushi Kawasaki Kosuke Doi Satoshi Ohta Asuka Inoue Hiroshi Murayama Source Type: research
Performance of Softcup ® menstrual cup and vulvovaginal swab samples for detection and quantification of genital cytokines
J Immunol Methods. 2024 Mar 4:113656. doi: 10.1016/j.jim.2024.113656. Online ahead of print.ABSTRACTCytokines are important mediators of immunity in the female genital tract, and their levels may be associated with various reproductive health outcomes. However, the measurement of cytokines and chemokines in vaginal fluid samples may be influenced by a variety of factors, each with the potential to affect the sensitivity and accuracy of the assay, including the interpretation and comparison of data. We measured and compared cytokine milieu in samples collected via Softcup® menstrual cup versus vulvovaginal swabs. One hundr...
Source: Journal of Immunological Methods - March 6, 2024 Category: Allergy & Immunology Authors: Nashlin Pillay Gugulethu Favourate Mzobe Marothi Letsoalo Asavela Olona Kama Andile Mtshali Stanley Nzuzo Magini Nikkishia Singh Vani Govender Natasha Samsunder Megeshinee Naidoo Dhayendre Moodley Cheryl Baxter Derseree Archary Sinaye Ngcapu Source Type: research
Development and basic performance verification of a rapid homogeneous bioassay for agonistic antibodies against the thyroid-stimulating hormone receptor
J Immunol Methods. 2024 Mar 4:113655. doi: 10.1016/j.jim.2024.113655. Online ahead of print.ABSTRACTGraves' disease is a type of autoimmune hyperthyroidism caused by thyroid-stimulating antibodies (TSAb).1 The combination of a porcine thyroid cell bioassay and cyclic adenosine monophosphate (cAMP) immunoassay (TSAb-enzyme immunoassay; EIA) is a clinically approved TSAb measurement method. Due to the requirement of multiple procedures and a long assay time of 6 h in the TSAb-EIA, a simplified and rapid assay is desired. Herein, we developed a rapid homogeneous TSAb bioassay (rapid-TSAb assay) using the human embryonic kidne...
Source: Journal of Immunological Methods - March 6, 2024 Category: Allergy & Immunology Authors: Motoki Hoshina Shiomi Ojima Atsushi Kawasaki Kosuke Doi Satoshi Ohta Asuka Inoue Hiroshi Murayama Source Type: research
High-resolution epitope mapping of commercial antibodies to ANCA antigens by yeast surface display
J Immunol Methods. 2024 Mar 1:113654. doi: 10.1016/j.jim.2024.113654. Online ahead of print.ABSTRACTEpitope mapping provides critical insight into antibody-antigen interactions. Epitope mapping of autoantibodies from patients with autoimmune diseases can help elucidate disease immunogenesis and guide the development of antigen-specific therapies. Similarly, epitope mapping of commercial antibodies targeting known autoantigens enables the use of those antibodies to test specific hypotheses. Anti-Neutrophil Cytoplasmic Autoantibody (ANCA) vasculitis results from the formation of autoantibodies to multiple autoantigens, inclu...
Source: Journal of Immunological Methods - March 3, 2024 Category: Allergy & Immunology Authors: John S Poulton Sajan Lamba Meghan Free Gang Xi Elizabeth McInnis Gabrielle Williams Stephan T Kudlacek David Thieker Brian Kuhlman Ronald Falk Source Type: research
High-resolution epitope mapping of commercial antibodies to ANCA antigens by yeast surface display
J Immunol Methods. 2024 Mar 1:113654. doi: 10.1016/j.jim.2024.113654. Online ahead of print.ABSTRACTEpitope mapping provides critical insight into antibody-antigen interactions. Epitope mapping of autoantibodies from patients with autoimmune diseases can help elucidate disease immunogenesis and guide the development of antigen-specific therapies. Similarly, epitope mapping of commercial antibodies targeting known autoantigens enables the use of those antibodies to test specific hypotheses. Anti-Neutrophil Cytoplasmic Autoantibody (ANCA) vasculitis results from the formation of autoantibodies to multiple autoantigens, inclu...
Source: Journal of Immunological Methods - March 3, 2024 Category: Allergy & Immunology Authors: John S Poulton Sajan Lamba Meghan Free Gang Xi Elizabeth McInnis Gabrielle Williams Stephan T Kudlacek David Thieker Brian Kuhlman Ronald Falk Source Type: research
High-resolution epitope mapping of commercial antibodies to ANCA antigens by yeast surface display
J Immunol Methods. 2024 Mar 1:113654. doi: 10.1016/j.jim.2024.113654. Online ahead of print.ABSTRACTEpitope mapping provides critical insight into antibody-antigen interactions. Epitope mapping of autoantibodies from patients with autoimmune diseases can help elucidate disease immunogenesis and guide the development of antigen-specific therapies. Similarly, epitope mapping of commercial antibodies targeting known autoantigens enables the use of those antibodies to test specific hypotheses. Anti-Neutrophil Cytoplasmic Autoantibody (ANCA) vasculitis results from the formation of autoantibodies to multiple autoantigens, inclu...
Source: Journal of Immunological Methods - March 3, 2024 Category: Allergy & Immunology Authors: John S Poulton Sajan Lamba Meghan Free Gang Xi Elizabeth McInnis Gabrielle Williams Stephan T Kudlacek David Thieker Brian Kuhlman Ronald Falk Source Type: research
An immunochromatographic test for serological diagnosis of scrub typhus
J Immunol Methods. 2024 Feb 29:113653. doi: 10.1016/j.jim.2024.113653. Online ahead of print.ABSTRACTA fluorescent immunochromatographic test (FM-ICT) was developed for rapid detection of anti-Orientia tsutsugamushi antibodies in serum samples. The FM-ICT was constructed based on the dual-antigen sandwich method. Truncated 56 kDa outer membrane protein of O. tsutsugamushi strain SJ, was expressed in E. coli and mixed with those of Ptan and Gillam strains. A thin line of the protein mixture was precisely sprayed across a nitrocellulose membrane making this the "Test" line. Polyclonal antibodies (pAbs) to O.tsutsugamushi wer...
Source: Journal of Immunological Methods - March 2, 2024 Category: Allergy & Immunology Authors: Shuhao Yan Qingyu Lu Yawei Lu Qingyuan Tao Bao Gao Sibo Wang Xusheng Cai Lele Ai Xiaohui Xiong Min Cao Weilong Tan Source Type: research
A comprehensive method for the phenotypical and functional characterization of recalled human memory B and T cells specific to vaccine antigens
This study not only establishes a rationale for the selection of methods to expand and detect antigen-specific lymphocyte subsets but also presents a means to quantify vaccine effectiveness by correlating serum antibody levels with preexisting memory cells within peripheral blood samples.PMID:38428517 | DOI:10.1016/j.jim.2024.113650 (Source: Journal of Immunological Methods)
Source: Journal of Immunological Methods - March 1, 2024 Category: Allergy & Immunology Authors: Czdari Lee Imtisal Imran Sara Thomas Mahyar Nouri-Shirazi Source Type: research
A comprehensive method for the phenotypical and functional characterization of recalled human memory B and T cells specific to vaccine antigens
This study not only establishes a rationale for the selection of methods to expand and detect antigen-specific lymphocyte subsets but also presents a means to quantify vaccine effectiveness by correlating serum antibody levels with preexisting memory cells within peripheral blood samples.PMID:38428517 | DOI:10.1016/j.jim.2024.113650 (Source: Journal of Immunological Methods)
Source: Journal of Immunological Methods - March 1, 2024 Category: Allergy & Immunology Authors: Czdari Lee Imtisal Imran Sara Thomas Mahyar Nouri-Shirazi Source Type: research
Methodological optimisation of thymocyte isolation and cryopreservation of human thymus samples
J Immunol Methods. 2024 Feb 26:113651. doi: 10.1016/j.jim.2024.113651. Online ahead of print.ABSTRACTPremature lymphocytes develop into non-autoreactive, mature naïve CD4+ or CD8+ T cells in the thymus before entering the circulation. However, in-depth characterization of human thymocyte development remains challenging due to limited availability of human thymus samples and the fragile nature of thymocyte populations. Thymocytes often do not survive cryopreservation and thawing procedures, especially the fragile CD4+CD8+ double positive population. It is generally recommended to use fresh human thymus tissue on the day of...
Source: Journal of Immunological Methods - February 28, 2024 Category: Allergy & Immunology Authors: Ruth R Hagen Calvin Xu Hui-Fern Koay Igor E Konstantinov Stuart P Berzins Katherine Kedzierska Carolien E van de Sandt Source Type: research
Methodological optimisation of thymocyte isolation and cryopreservation of human thymus samples
J Immunol Methods. 2024 Feb 26:113651. doi: 10.1016/j.jim.2024.113651. Online ahead of print.ABSTRACTPremature lymphocytes develop into non-autoreactive, mature naïve CD4+ or CD8+ T cells in the thymus before entering the circulation. However, in-depth characterization of human thymocyte development remains challenging due to limited availability of human thymus samples and the fragile nature of thymocyte populations. Thymocytes often do not survive cryopreservation and thawing procedures, especially the fragile CD4+CD8+ double positive population. It is generally recommended to use fresh human thymus tissue on the day of...
Source: Journal of Immunological Methods - February 28, 2024 Category: Allergy & Immunology Authors: Ruth R Hagen Calvin Xu Hui-Fern Koay Igor E Konstantinov Stuart P Berzins Katherine Kedzierska Carolien E van de Sandt Source Type: research
Methodological optimisation of thymocyte isolation and cryopreservation of human thymus samples
J Immunol Methods. 2024 Feb 26:113651. doi: 10.1016/j.jim.2024.113651. Online ahead of print.ABSTRACTPremature lymphocytes develop into non-autoreactive, mature naïve CD4+ or CD8+ T cells in the thymus before entering the circulation. However, in-depth characterization of human thymocyte development remains challenging due to limited availability of human thymus samples and the fragile nature of thymocyte populations. Thymocytes often do not survive cryopreservation and thawing procedures, especially the fragile CD4+CD8+ double positive population. It is generally recommended to use fresh human thymus tissue on the day of...
Source: Journal of Immunological Methods - February 28, 2024 Category: Allergy & Immunology Authors: Ruth R Hagen Calvin Xu Hui-Fern Koay Igor E Konstantinov Stuart P Berzins Katherine Kedzierska Carolien E van de Sandt Source Type: research
True volumetric counting of CD34+ cells using flow cytometry
In this study we have used a direct true volumetric counting of CD34+ cells under continuous flow pump to overcome potential drawbacks with impact in rare cell analysis. To confirm this hypothesis, we have compared the results of CD34+ cell enumeration using non-volumetric vs. volumetric systems with FC500 (Beckman Coulter) and Attune NxT (ThermoFisher) flow cytometers, respectively, in mobilized peripheral blood samples. No statistically significant differences were observed between measurements of CD34+ cells using beads, when the FC500 and Attune NxT absolute counting values were compared, or when CD34+ counts were comp...
Source: Journal of Immunological Methods - February 23, 2024 Category: Allergy & Immunology Authors: Laura G Rico Jorge Bardina Roser Salvia Michael D Ward Jolene A Bradford Jordi Petriz Source Type: research
True volumetric counting of CD34+ cells using flow cytometry
In this study we have used a direct true volumetric counting of CD34+ cells under continuous flow pump to overcome potential drawbacks with impact in rare cell analysis. To confirm this hypothesis, we have compared the results of CD34+ cell enumeration using non-volumetric vs. volumetric systems with FC500 (Beckman Coulter) and Attune NxT (ThermoFisher) flow cytometers, respectively, in mobilized peripheral blood samples. No statistically significant differences were observed between measurements of CD34+ cells using beads, when the FC500 and Attune NxT absolute counting values were compared, or when CD34+ counts were comp...
Source: Journal of Immunological Methods - February 23, 2024 Category: Allergy & Immunology Authors: Laura G Rico Jorge Bardina Roser Salvia Michael D Ward Jolene A Bradford Jordi Petriz Source Type: research
