Stability of anti-drug antibodies in human serum samples
J Immunol Methods. 2024 Feb;525:113616. doi: 10.1016/j.jim.2024.113616. Epub 2024 Jan 10.ABSTRACTStability of anti-drug antibodies (ADAs) is important as ADA-analysis should be reliable over time at different storage conditions. Stability of anti-insulin antibodies in serum samples was assessed after short-term storage at different temperatures and after long-term storage at -20 °C. Correlation between measurements was tested and acceptance criteria for incurred sample reanalysis were applied. ADAs were stable after 72 h at 22 °C, after 2 weeks at 4 °C, and after 6.3 years at -20 °C. The study confirms that ADAs in ser...
Source: Journal of Immunological Methods - January 11, 2024 Category: Allergy & Immunology Authors: Helene Solberg Christina Bartholdy Lone Hummelsh øj Landsy Lars Ole Andresen Source Type: research
Development of optimized cytotoxicity assays for assessing the antitumor potential of CAR-T cells
J Immunol Methods. 2024 Feb;525:113603. doi: 10.1016/j.jim.2023.113603. Epub 2023 Dec 24.ABSTRACTCAR-T cells are T cells expressing a chimeric antigen receptor (CAR) rendering them capable of killing tumor cells after recognition of a target antigen. CD19 CAR-T cells have revolutionized the treatment of hematological malignancies. Their function is typically assessed by cytotoxicity assays using human allogeneic cell lines expressing the target antigen CD19 such as Nalm-6. However, an alloreactive reaction is observed with these cells, leading to a CD19-independent killing. To address this issue, we developed a fluorescenc...
Source: Journal of Immunological Methods - December 26, 2023 Category: Allergy & Immunology Authors: Misa Eugene-Norbert Alexis Cuffel Gaetan Riou Laetitia Jean Clara Blondel Justine Dehayes Aur élie Bisson Camille Giverne Emilie Brotin Christophe Denoyelle Laurent Poulain Olivier Boyer J érémie Martinet Jean-Baptiste Latouche Source Type: research
Development of optimized cytotoxicity assays for assessing the antitumor potential of CAR-T cells
J Immunol Methods. 2023 Dec 24;525:113603. doi: 10.1016/j.jim.2023.113603. Online ahead of print.ABSTRACTCAR-T cells are T cells expressing a chimeric antigen receptor (CAR) rendering them capable of killing tumor cells after recognition of a target antigen. CD19 CAR-T cells have revolutionized the treatment of hematological malignancies. Their function is typically assessed by cytotoxicity assays using human allogeneic cell lines expressing the target antigen CD19 such as Nalm-6. However, an alloreactive reaction is observed with these cells, leading to a CD19-independent killing. To address this issue, we developed a flu...
Source: Journal of Immunological Methods - December 26, 2023 Category: Allergy & Immunology Authors: Misa Eugene-Norbert Alexis Cuffel Gaetan Riou Laetitia Jean Clara Blondel Justine Dehayes Aur élie Bisson Camille Giverne Emilie Brotin Christophe Denoyelle Laurent Poulain Olivier Boyer J érémie Martinet Jean-Baptiste Latouche Source Type: research
Development of optimized cytotoxicity assays for assessing the antitumor potential of CAR-T cells
J Immunol Methods. 2023 Dec 24;525:113603. doi: 10.1016/j.jim.2023.113603. Online ahead of print.ABSTRACTCAR-T cells are T cells expressing a chimeric antigen receptor (CAR) rendering them capable of killing tumor cells after recognition of a target antigen. CD19 CAR-T cells have revolutionized the treatment of hematological malignancies. Their function is typically assessed by cytotoxicity assays using human allogeneic cell lines expressing the target antigen CD19 such as Nalm-6. However, an alloreactive reaction is observed with these cells, leading to a CD19-independent killing. To address this issue, we developed a flu...
Source: Journal of Immunological Methods - December 26, 2023 Category: Allergy & Immunology Authors: Misa Eugene-Norbert Alexis Cuffel Gaetan Riou Laetitia Jean Clara Blondel Justine Dehayes Aur élie Bisson Camille Giverne Emilie Brotin Christophe Denoyelle Laurent Poulain Olivier Boyer J érémie Martinet Jean-Baptiste Latouche Source Type: research
Development of optimized cytotoxicity assays for assessing the antitumor potential of CAR-T cells
J Immunol Methods. 2023 Dec 24;525:113603. doi: 10.1016/j.jim.2023.113603. Online ahead of print.ABSTRACTCAR-T cells are T cells expressing a chimeric antigen receptor (CAR) rendering them capable of killing tumor cells after recognition of a target antigen. CD19 CAR-T cells have revolutionized the treatment of hematological malignancies. Their function is typically assessed by cytotoxicity assays using human allogeneic cell lines expressing the target antigen CD19 such as Nalm-6. However, an alloreactive reaction is observed with these cells, leading to a CD19-independent killing. To address this issue, we developed a flu...
Source: Journal of Immunological Methods - December 26, 2023 Category: Allergy & Immunology Authors: Misa Eugene-Norbert Alexis Cuffel Gaetan Riou Laetitia Jean Clara Blondel Justine Dehayes Aur élie Bisson Camille Giverne Emilie Brotin Christophe Denoyelle Laurent Poulain Olivier Boyer J érémie Martinet Jean-Baptiste Latouche Source Type: research
Development of optimized cytotoxicity assays for assessing the antitumor potential of CAR-T cells
J Immunol Methods. 2023 Dec 24;525:113603. doi: 10.1016/j.jim.2023.113603. Online ahead of print.ABSTRACTCAR-T cells are T cells expressing a chimeric antigen receptor (CAR) rendering them capable of killing tumor cells after recognition of a target antigen. CD19 CAR-T cells have revolutionized the treatment of hematological malignancies. Their function is typically assessed by cytotoxicity assays using human allogeneic cell lines expressing the target antigen CD19 such as Nalm-6. However, an alloreactive reaction is observed with these cells, leading to a CD19-independent killing. To address this issue, we developed a flu...
Source: Journal of Immunological Methods - December 26, 2023 Category: Allergy & Immunology Authors: Misa Eugene-Norbert Alexis Cuffel Gaetan Riou Laetitia Jean Clara Blondel Justine Dehayes Aur élie Bisson Camille Giverne Emilie Brotin Christophe Denoyelle Laurent Poulain Olivier Boyer J érémie Martinet Jean-Baptiste Latouche Source Type: research
Development of optimized cytotoxicity assays for assessing the antitumor potential of CAR-T cells
J Immunol Methods. 2023 Dec 24:113603. doi: 10.1016/j.jim.2023.113603. Online ahead of print.ABSTRACTCAR-T cells are T cell expressing a chimeric antigen receptor (CAR) rendering them capable of killing tumor cells after recognition of a target antigen. CD19 CAR-T cells have revolutionized the treatment of hematological malignancies. Their function is typically assessed by cytotoxicity assays using human allogeneic cell lines expressing the target antigen CD19 such as Nalm-6. However, an alloreactive reaction is observed with these cells, leading to a CD19-independent killing. To address this issue, we developed a fluoresc...
Source: Journal of Immunological Methods - December 26, 2023 Category: Allergy & Immunology Authors: Misa Eugene Norbert Alexis Cuffel Gaetan Riou Laetitia Jean Clara Blondel Justine Dehayes Aur élie Bisson Camille Giverne Emilie Brotin Christophe Denoyelle Laurent Poulain Olivier Boyer J érémie Martinet Jean-Baptiste Latouche Source Type: research
Development of optimized cytotoxicity assays for assessing the antitumor potential of CAR-T cells
J Immunol Methods. 2023 Dec 24:113603. doi: 10.1016/j.jim.2023.113603. Online ahead of print.ABSTRACTCAR-T cells are T cell expressing a chimeric antigen receptor (CAR) rendering them capable of killing tumor cells after recognition of a target antigen. CD19 CAR-T cells have revolutionized the treatment of hematological malignancies. Their function is typically assessed by cytotoxicity assays using human allogeneic cell lines expressing the target antigen CD19 such as Nalm-6. However, an alloreactive reaction is observed with these cells, leading to a CD19-independent killing. To address this issue, we developed a fluoresc...
Source: Journal of Immunological Methods - December 26, 2023 Category: Allergy & Immunology Authors: Misa Eugene Norbert Alexis Cuffel Gaetan Riou Laetitia Jean Clara Blondel Justine Dehayes Aur élie Bisson Camille Giverne Emilie Brotin Christophe Denoyelle Laurent Poulain Olivier Boyer J érémie Martinet Jean-Baptiste Latouche Source Type: research
Development of optimized cytotoxicity assays for assessing the antitumor potential of CAR-T cells
J Immunol Methods. 2023 Dec 24:113603. doi: 10.1016/j.jim.2023.113603. Online ahead of print.ABSTRACTCAR-T cells are T cell expressing a chimeric antigen receptor (CAR) rendering them capable of killing tumor cells after recognition of a target antigen. CD19 CAR-T cells have revolutionized the treatment of hematological malignancies. Their function is typically assessed by cytotoxicity assays using human allogeneic cell lines expressing the target antigen CD19 such as Nalm-6. However, an alloreactive reaction is observed with these cells, leading to a CD19-independent killing. To address this issue, we developed a fluoresc...
Source: Journal of Immunological Methods - December 26, 2023 Category: Allergy & Immunology Authors: Misa Eugene Norbert Alexis Cuffel Gaetan Riou Laetitia Jean Clara Blondel Justine Dehayes Aur élie Bisson Camille Giverne Emilie Brotin Christophe Denoyelle Laurent Poulain Olivier Boyer J érémie Martinet Jean-Baptiste Latouche Source Type: research
Development of optimized cytotoxicity assays for assessing the antitumor potential of CAR-T cells
J Immunol Methods. 2023 Dec 24:113603. doi: 10.1016/j.jim.2023.113603. Online ahead of print.ABSTRACTCAR-T cells are T cell expressing a chimeric antigen receptor (CAR) rendering them capable of killing tumor cells after recognition of a target antigen. CD19 CAR-T cells have revolutionized the treatment of hematological malignancies. Their function is typically assessed by cytotoxicity assays using human allogeneic cell lines expressing the target antigen CD19 such as Nalm-6. However, an alloreactive reaction is observed with these cells, leading to a CD19-independent killing. To address this issue, we developed a fluoresc...
Source: Journal of Immunological Methods - December 26, 2023 Category: Allergy & Immunology Authors: Misa Eugene Norbert Alexis Cuffel Gaetan Riou Laetitia Jean Clara Blondel Justine Dehayes Aur élie Bisson Camille Giverne Emilie Brotin Christophe Denoyelle Laurent Poulain Olivier Boyer J érémie Martinet Jean-Baptiste Latouche Source Type: research
Development of optimized cytotoxicity assays for assessing the antitumor potential of CAR-T cells
J Immunol Methods. 2023 Dec 24:113603. doi: 10.1016/j.jim.2023.113603. Online ahead of print.ABSTRACTCAR-T cells are T cell expressing a chimeric antigen receptor (CAR) rendering them capable of killing tumor cells after recognition of a target antigen. CD19 CAR-T cells have revolutionized the treatment of hematological malignancies. Their function is typically assessed by cytotoxicity assays using human allogeneic cell lines expressing the target antigen CD19 such as Nalm-6. However, an alloreactive reaction is observed with these cells, leading to a CD19-independent killing. To address this issue, we developed a fluoresc...
Source: Journal of Immunological Methods - December 26, 2023 Category: Allergy & Immunology Authors: Misa Eugene Norbert Alexis Cuffel Gaetan Riou Laetitia Jean Clara Blondel Justine Dehayes Aur élie Bisson Camille Giverne Emilie Brotin Christophe Denoyelle Laurent Poulain Olivier Boyer J érémie Martinet Jean-Baptiste Latouche Source Type: research
Development of optimized cytotoxicity assays for assessing the antitumor potential of CAR-T cells
J Immunol Methods. 2023 Dec 24:113603. doi: 10.1016/j.jim.2023.113603. Online ahead of print.ABSTRACTCAR-T cells are T cell expressing a chimeric antigen receptor (CAR) rendering them capable of killing tumor cells after recognition of a target antigen. CD19 CAR-T cells have revolutionized the treatment of hematological malignancies. Their function is typically assessed by cytotoxicity assays using human allogeneic cell lines expressing the target antigen CD19 such as Nalm-6. However, an alloreactive reaction is observed with these cells, leading to a CD19-independent killing. To address this issue, we developed a fluoresc...
Source: Journal of Immunological Methods - December 26, 2023 Category: Allergy & Immunology Authors: Misa Eugene Norbert Alexis Cuffel Gaetan Riou Laetitia Jean Clara Blondel Justine Dehayes Aur élie Bisson Camille Giverne Emilie Brotin Christophe Denoyelle Laurent Poulain Olivier Boyer J érémie Martinet Jean-Baptiste Latouche Source Type: research
A live mammalian cells electroporation array for on-chip immunofluorescence
J Immunol Methods. 2023 Dec 23:113607. doi: 10.1016/j.jim.2023.113607. Online ahead of print.ABSTRACTThe detection of intracellular proteins in vitro is commonly realized with immunofluorescence techniques, through which antibodies or markers are delivered into fixed cells and recognize specific proteins. Many innovative techniques, however, avoid cells fixation by chemical compounds and, among the others, electroporation is widely used. Here we demonstrate that in situ electroporation on thin film SiO2 capacitive microelectrodes can be realized with high efficiency to deliver fluorescent markers and antibodies into mammal...
Source: Journal of Immunological Methods - December 25, 2023 Category: Allergy & Immunology Authors: Maschietto Marta Girardi Stefano Gagliano Onelia Vassanelli Stefano Source Type: research
Antibody engineering to generate anti-tumor-associated glycoprotein 72 mouse recombinant CC49 IgG with improved solubility, purity, and thermal stability
J Immunol Methods. 2023 Dec 23:113606. doi: 10.1016/j.jim.2023.113606. Online ahead of print.ABSTRACTTumor-associated glycoprotein 72 (TAG-72) is a mucin that is overexpressed heterogeneously on the surface of cancer cells, and is a potential target for immunotherapies for various cancer types. As a tumor marker, TAG-72 is measured with the cancer antigen (CA) 72-4 immunoassay. The murine monoclonal antibody (mAb) CC49 is a second-generation IgG that targets an antigen on TAG-72; however, CC49 has an unfavorable propensity to aggregate, which results in antibody impurity, instability, and low solubility and thus low potenc...
Source: Journal of Immunological Methods - December 25, 2023 Category: Allergy & Immunology Authors: Zhihong Lin Bailin Tu Philip M Hemken A Scott Muerhoff Source Type: research
A live mammalian cells electroporation array for on-chip immunofluorescence
J Immunol Methods. 2023 Dec 23:113607. doi: 10.1016/j.jim.2023.113607. Online ahead of print.ABSTRACTThe detection of intracellular proteins in vitro is commonly realized with immunofluorescence techniques, through which antibodies or markers are delivered into fixed cells and recognize specific proteins. Many innovative techniques, however, avoid cells fixation by chemical compounds and, among the others, electroporation is widely used. Here we demonstrate that in situ electroporation on thin film SiO2 capacitive microelectrodes can be realized with high efficiency to deliver fluorescent markers and antibodies into mammal...
Source: Journal of Immunological Methods - December 25, 2023 Category: Allergy & Immunology Authors: Maschietto Marta Girardi Stefano Gagliano Onelia Vassanelli Stefano Source Type: research
