Parameters of TNF receptor co-expression in allergic and autoimmune processes: Differences and diagnostic significance
J Immunol Methods. 2023 Jul 17:113525. doi: 10.1016/j.jim.2023.113525. Online ahead of print.ABSTRACTThe authors used a method quantitative estimation density of TNFR1/TNFR2 on cells by flow cytometry with calibration particles, which allowed them to estimate the absolute number of receptors on cells regardless of the type of flow cytometer. The TNF receptor expression parameters were used to determine their association with the fact of disease and to build diagnostic models. The proposed methodological approach using a combination of flow cytometry and mathematical modeling techniques represents a promising direction for ...
Source: Journal of Immunological Methods - July 19, 2023 Category: Allergy & Immunology Authors: Alina Alshevskaya Julia Zhukova Julia Lopatnikova Nadezhda Shkaruba Oksana Chumasova Alexey Sizikov Daria Demina Vera Nepomniashchikh Viktor Gladkikh Sergey Sennikov Source Type: research
Parameters of TNF receptor co-expression in allergic and autoimmune processes: Differences and diagnostic significance
J Immunol Methods. 2023 Jul 17:113525. doi: 10.1016/j.jim.2023.113525. Online ahead of print.ABSTRACTThe authors used a method quantitative estimation density of TNFR1/TNFR2 on cells by flow cytometry with calibration particles, which allowed them to estimate the absolute number of receptors on cells regardless of the type of flow cytometer. The TNF receptor expression parameters were used to determine their association with the fact of disease and to build diagnostic models. The proposed methodological approach using a combination of flow cytometry and mathematical modeling techniques represents a promising direction for ...
Source: Journal of Immunological Methods - July 19, 2023 Category: Allergy & Immunology Authors: Alina Alshevskaya Julia Zhukova Julia Lopatnikova Nadezhda Shkaruba Oksana Chumasova Alexey Sizikov Daria Demina Vera Nepomniashchikh Viktor Gladkikh Sergey Sennikov Source Type: research
Parameters of TNF receptor co-expression in allergic and autoimmune processes: Differences and diagnostic significance
J Immunol Methods. 2023 Jul 17:113525. doi: 10.1016/j.jim.2023.113525. Online ahead of print.ABSTRACTThe authors used a method quantitative estimation density of TNFR1/TNFR2 on cells by flow cytometry with calibration particles, which allowed them to estimate the absolute number of receptors on cells regardless of the type of flow cytometer. The TNF receptor expression parameters were used to determine their association with the fact of disease and to build diagnostic models. The proposed methodological approach using a combination of flow cytometry and mathematical modeling techniques represents a promising direction for ...
Source: Journal of Immunological Methods - July 19, 2023 Category: Allergy & Immunology Authors: Alina Alshevskaya Julia Zhukova Julia Lopatnikova Nadezhda Shkaruba Oksana Chumasova Alexey Sizikov Daria Demina Vera Nepomniashchikh Viktor Gladkikh Sergey Sennikov Source Type: research
Parameters of TNF receptor co-expression in allergic and autoimmune processes: Differences and diagnostic significance
J Immunol Methods. 2023 Jul 17:113525. doi: 10.1016/j.jim.2023.113525. Online ahead of print.ABSTRACTThe authors used a method quantitative estimation density of TNFR1/TNFR2 on cells by flow cytometry with calibration particles, which allowed them to estimate the absolute number of receptors on cells regardless of the type of flow cytometer. The TNF receptor expression parameters were used to determine their association with the fact of disease and to build diagnostic models. The proposed methodological approach using a combination of flow cytometry and mathematical modeling techniques represents a promising direction for ...
Source: Journal of Immunological Methods - July 19, 2023 Category: Allergy & Immunology Authors: Alina Alshevskaya Julia Zhukova Julia Lopatnikova Nadezhda Shkaruba Oksana Chumasova Alexey Sizikov Daria Demina Vera Nepomniashchikh Viktor Gladkikh Sergey Sennikov Source Type: research
Parameters of TNF receptor co-expression in allergic and autoimmune processes: Differences and diagnostic significance
J Immunol Methods. 2023 Jul 17:113525. doi: 10.1016/j.jim.2023.113525. Online ahead of print.ABSTRACTThe authors used a method quantitative estimation density of TNFR1/TNFR2 on cells by flow cytometry with calibration particles, which allowed them to estimate the absolute number of receptors on cells regardless of the type of flow cytometer. The TNF receptor expression parameters were used to determine their association with the fact of disease and to build diagnostic models. The proposed methodological approach using a combination of flow cytometry and mathematical modeling techniques represents a promising direction for ...
Source: Journal of Immunological Methods - July 19, 2023 Category: Allergy & Immunology Authors: Alina Alshevskaya Julia Zhukova Julia Lopatnikova Nadezhda Shkaruba Oksana Chumasova Alexey Sizikov Daria Demina Vera Nepomniashchikh Viktor Gladkikh Sergey Sennikov Source Type: research
Cross-reactivity of T cell-specific antibodies in the bank vole (Myodes glareolus)
J Immunol Methods. 2023 Jul 16:113524. doi: 10.1016/j.jim.2023.113524. Online ahead of print.ABSTRACTThe bank vole is a common Cricetidae rodent that is a reservoir of several zoonotic pathogens and an emerging model in eco-immunology. Here, we add to a developing immunological toolkit for this species by testing the cross-reactivity of commercially available monoclonal antibodies (mAbs) to the bank vole lymphocyte differentiation molecules and a transcription factor. We show that a combination of mAbs against CD4, CD3, and Foxp3 allows flow cytometric distinction of the main subsets of T cells: putative helper CD4+, cytot...
Source: Journal of Immunological Methods - July 18, 2023 Category: Allergy & Immunology Authors: Migalska Magdalena W ęglarczyk Kazimierz M ężyk-Kopeć Renata Baliga-Klimczyk Katarzyna Homa Joanna Source Type: research
Cross-reactivity of T cell-specific antibodies in the bank vole (Myodes glareolus)
J Immunol Methods. 2023 Jul 16:113524. doi: 10.1016/j.jim.2023.113524. Online ahead of print.ABSTRACTThe bank vole is a common Cricetidae rodent that is a reservoir of several zoonotic pathogens and an emerging model in eco-immunology. Here, we add to a developing immunological toolkit for this species by testing the cross-reactivity of commercially available monoclonal antibodies (mAbs) to the bank vole lymphocyte differentiation molecules and a transcription factor. We show that a combination of mAbs against CD4, CD3, and Foxp3 allows flow cytometric distinction of the main subsets of T cells: putative helper CD4+, cytot...
Source: Journal of Immunological Methods - July 18, 2023 Category: Allergy & Immunology Authors: Migalska Magdalena W ęglarczyk Kazimierz M ężyk-Kopeć Renata Baliga-Klimczyk Katarzyna Homa Joanna Source Type: research
Comparison of capillary blood self-collection using the Tasso-SST device with venous phlebotomy for anti-SARS-CoV-2 antibody measurement
J Immunol Methods. 2023 Jul 8;520:113523. doi: 10.1016/j.jim.2023.113523. Online ahead of print.ABSTRACTMeasuring seroprevalence over time is a valuable epidemiological tool for improving our understanding of COVID-19 immunity. Due to the large number of collections required for population surveillance as well as concerns about potential infection risk to the collectors, self-collection approaches are being increasingly pursued. To advance this methodology, we collected paired venous and capillary blood samples by routine phlebotomy and Tasso-SST device respectively from 26 participants and measured total immunoglobulin (I...
Source: Journal of Immunological Methods - July 9, 2023 Category: Allergy & Immunology Authors: Elise R King Haley E Garrett Haley Abernathy Caitlin A Cassidy Cameron R Cabell Bonnie E Shook-Sa Jonathan J Juliano Ross M Boyce Allison E Aiello Emily J Ciccone Source Type: research
Development and characterization of a latex turbidimetric immunoassay using rabbit anti-CRP single-chain Fv antibodies
In this study, we developed and demonstrated a latex turbidimetric immunoassay (LTIA) using latex beads immobilized with rabbit monoclonal single-chain variable fragments (scFvs) selected from an scFv-displayed phage library. Sixty-five different anti-c-reactive protein (anti-CRP) scFv clones were identified after biopanning selection using antigen-coupled multi-lamellar vesicles. By ranking antigen-binding clones using the apparent dissociation rate constant (appkoff) as a sorting index, scFv clones with a dissociation constant (KD free) ranging from 4.07 × 10-9 M to 1.21 × 10-11 M were isolated. Among them, three candi...
Source: Journal of Immunological Methods - July 9, 2023 Category: Allergy & Immunology Authors: Yoichi Kumada Reina Tanibata Kimitaka Yamamoto Hiroshi Noguchi Alessandro Angelini Jun-Ichi Horiuchi Source Type: research
Comparison of capillary blood self-collection using the Tasso-SST device with venous phlebotomy for anti-SARS-CoV-2 antibody measurement
J Immunol Methods. 2023 Jul 7:113523. doi: 10.1016/j.jim.2023.113523. Online ahead of print.ABSTRACTMeasuring seroprevalence over time is a valuable epidemiological tool for improving our understanding of COVID-19 immunity. Due to the large number of collections required for population surveillance as well as concerns about potential infection risk to the collectors, self-collection approaches are being increasingly pursued. To advance this methodology, we collected paired venous and capillary blood samples by routine phlebotomy and Tasso-SST device respectively from 26 participants and measured total immunoglobulin (Ig) a...
Source: Journal of Immunological Methods - July 9, 2023 Category: Allergy & Immunology Authors: Elise R King Haley E Garrett Haley Abernathy Caitlin A Cassidy Cameron R Cabell Bonnie E Shook-Sa Jonathan J Juliano Ross M Boyce Allison E Aiello Emily J Ciccone Source Type: research
Development and characterization of a latex turbidimetric immunoassay using rabbit anti-CRP single-chain Fv antibodies
In this study, we developed and demonstrated a latex turbidimetric immunoassay (LTIA) using latex beads immobilized with rabbit monoclonal single-chain variable fragments (scFvs) selected from an scFv-displayed phage library. Sixty-five different anti-c-reactive protein (anti-CRP) scFv clones were identified after biopanning selection using antigen-coupled multi-lamellar vesicles. By ranking antigen-binding clones using the apparent dissociation rate constant (appkoff) as a sorting index, scFv clones with a dissociation constant (KD free) ranging from 4.07 × 10-9 M to 1.21 × 10-11 M were isolated. Among them, three candi...
Source: Journal of Immunological Methods - July 9, 2023 Category: Allergy & Immunology Authors: Yoichi Kumada Reina Tanibata Kimitaka Yamamoto Hiroshi Noguchi Alessandro Angelini Jun-Ichi Horiuchi Source Type: research
Comparison of capillary blood self-collection using the Tasso-SST device with venous phlebotomy for anti-SARS-CoV-2 antibody measurement
J Immunol Methods. 2023 Jul 7:113523. doi: 10.1016/j.jim.2023.113523. Online ahead of print.ABSTRACTMeasuring seroprevalence over time is a valuable epidemiological tool for improving our understanding of COVID-19 immunity. Due to the large number of collections required for population surveillance as well as concerns about potential infection risk to the collectors, self-collection approaches are being increasingly pursued. To advance this methodology, we collected paired venous and capillary blood samples by routine phlebotomy and Tasso-SST device respectively from 26 participants and measured total immunoglobulin (Ig) a...
Source: Journal of Immunological Methods - July 9, 2023 Category: Allergy & Immunology Authors: Elise R King Haley E Garrett Haley Abernathy Caitlin A Cassidy Cameron R Cabell Bonnie E Shook-Sa Jonathan J Juliano Ross M Boyce Allison E Aiello Emily J Ciccone Source Type: research
Development and characterization of a latex turbidimetric immunoassay using rabbit anti-CRP single-chain Fv antibodies
In this study, we developed and demonstrated a latex turbidimetric immunoassay (LTIA) using latex beads immobilized with rabbit monoclonal single-chain variable fragments (scFvs) selected from an scFv-displayed phage library. Sixty-five different anti-c-reactive protein (anti-CRP) scFv clones were identified after biopanning selection using antigen-coupled multi-lamellar vesicles. By ranking antigen-binding clones using the apparent dissociation rate constant (appkoff) as a sorting index, scFv clones with a dissociation constant (KD free) ranging from 4.07 × 10-9 M to 1.21 × 10-11 M were isolated. Among them, three candi...
Source: Journal of Immunological Methods - July 9, 2023 Category: Allergy & Immunology Authors: Yoichi Kumada Reina Tanibata Kimitaka Yamamoto Hiroshi Noguchi Alessandro Angelini Jun-Ichi Horiuchi Source Type: research
Comparison of capillary blood self-collection using the Tasso-SST device with venous phlebotomy for anti-SARS-CoV-2 antibody measurement
J Immunol Methods. 2023 Jul 7:113523. doi: 10.1016/j.jim.2023.113523. Online ahead of print.ABSTRACTMeasuring seroprevalence over time is a valuable epidemiological tool for improving our understanding of COVID-19 immunity. Due to the large number of collections required for population surveillance as well as concerns about potential infection risk to the collectors, self-collection approaches are being increasingly pursued. To advance this methodology, we collected paired venous and capillary blood samples by routine phlebotomy and Tasso-SST device respectively from 26 participants and measured total immunoglobulin (Ig) a...
Source: Journal of Immunological Methods - July 9, 2023 Category: Allergy & Immunology Authors: Elise R King Haley E Garrett Haley Abernathy Caitlin A Cassidy Cameron R Cabell Bonnie E Shook-Sa Jonathan J Juliano Ross M Boyce Allison E Aiello Emily J Ciccone Source Type: research
Development and characterization of a latex turbidimetric immunoassay using rabbit anti-CRP single-chain Fv antibodies
In this study, we developed and demonstrated a latex turbidimetric immunoassay (LTIA) using latex beads immobilized with rabbit monoclonal single-chain variable fragments (scFvs) selected from an scFv-displayed phage library. Sixty-five different anti-c-reactive protein (anti-CRP) scFv clones were identified after biopanning selection using antigen-coupled multi-lamellar vesicles. By ranking antigen-binding clones using the apparent dissociation rate constant (appkoff) as a sorting index, scFv clones with a dissociation constant (KD free) ranging from 4.07 × 10-9 M to 1.21 × 10-11 M were isolated. Among them, three candi...
Source: Journal of Immunological Methods - July 9, 2023 Category: Allergy & Immunology Authors: Yoichi Kumada Reina Tanibata Kimitaka Yamamoto Hiroshi Noguchi Alessandro Angelini Jun-Ichi Horiuchi Source Type: research
