Diagnosis of septic shock by serum measurement of human neutrophil lipocalin by a rapid homogeneous assay
CONCLUSIONS: As an assessment tool, we found that our AlphaLISA had good consistency with an ELISA and had several other advantages, including requiring a shorter processing time and detecting a wider range of serum HNL concentrations. Monitoring serum HNL levels of patients admitted to the ICU might be useful in distinguishing sepsis patients who have septic shock from other sepsis patients, indicating its value in the prediction of sepsis patient prognosis.PMID:37774777 | DOI:10.1016/j.jim.2023.113570 (Source: Journal of Immunological Methods)
Source: Journal of Immunological Methods - September 29, 2023 Category: Allergy & Immunology Authors: Huijun Zong Xueyi Shang Xin Wang Ting Chen Ye Wang Yuhao Ren Yongqiang Jiang Yan Li Qingyu Lv Peng Liu Source Type: research
Impact of bridge heterology on functional parameters of ELISA for 17 α-methyltestosterone
In this study, we have developed bridge heterologous ELISA for the detection of 17α- Methyltestosterone by incorporating aromatic spacers between 17α-Methyltestosterone-3-Carboxymethyloxime and Horseradish peroxidase label through N-hydroxysuccinimide mediated carbodiimide reaction method. The immunogen 17α-Methyltestosterone-3-Carboxymethyloxime-Bovine serum albumin used to generate the antibody was also prepared by the N-hydroxysuccinimide mediated carbodiimide reaction without using any spacer. We have studied the impact of bridge/aromatic spacers on functional parameters i.e. sensitivity, affinity and ED50 of the br...
Source: Journal of Immunological Methods - September 29, 2023 Category: Allergy & Immunology Authors: Divya Verma T G Shrivastav Sonu Chand Thakur Source Type: research
Diagnosis of septic shock by serum measurement of human neutrophil lipocalin by a rapid homogeneous assay
CONCLUSIONS: As an assessment tool, we found that our AlphaLISA had good consistency with an ELISA and had several other advantages, including requiring a shorter processing time and detecting a wider range of serum HNL concentrations. Monitoring serum HNL levels of patients admitted to the ICU might be useful in distinguishing sepsis patients who have septic shock from other sepsis patients, indicating its value in the prediction of sepsis patient prognosis.PMID:37774777 | DOI:10.1016/j.jim.2023.113570 (Source: Journal of Immunological Methods)
Source: Journal of Immunological Methods - September 29, 2023 Category: Allergy & Immunology Authors: Huijun Zong Xueyi Shang Xin Wang Ting Chen Ye Wang Yuhao Ren Yongqiang Jiang Yan Li Qingyu Lv Peng Liu Source Type: research
Impact of bridge heterology on functional parameters of ELISA for 17 α-methyltestosterone
In this study, we have developed bridge heterologous ELISA for the detection of 17α- Methyltestosterone by incorporating aromatic spacers between 17α-Methyltestosterone-3-Carboxymethyloxime and Horseradish peroxidase label through N-hydroxysuccinimide mediated carbodiimide reaction method. The immunogen 17α-Methyltestosterone-3-Carboxymethyloxime-Bovine serum albumin used to generate the antibody was also prepared by the N-hydroxysuccinimide mediated carbodiimide reaction without using any spacer. We have studied the impact of bridge/aromatic spacers on functional parameters i.e. sensitivity, affinity and ED50 of the br...
Source: Journal of Immunological Methods - September 29, 2023 Category: Allergy & Immunology Authors: Divya Verma T G Shrivastav Sonu Chand Thakur Source Type: research
Diagnosis of septic shock by serum measurement of human neutrophil lipocalin by a rapid homogeneous assay
CONCLUSIONS: As an assessment tool, we found that our AlphaLISA had good consistency with an ELISA and had several other advantages, including requiring a shorter processing time and detecting a wider range of serum HNL concentrations. Monitoring serum HNL levels of patients admitted to the ICU might be useful in distinguishing sepsis patients who have septic shock from other sepsis patients, indicating its value in the prediction of sepsis patient prognosis.PMID:37774777 | DOI:10.1016/j.jim.2023.113570 (Source: Journal of Immunological Methods)
Source: Journal of Immunological Methods - September 29, 2023 Category: Allergy & Immunology Authors: Huijun Zong Xueyi Shang Xin Wang Ting Chen Ye Wang Yuhao Ren Yongqiang Jiang Yan Li Qingyu Lv Peng Liu Source Type: research
Impact of bridge heterology on functional parameters of ELISA for 17 α-methyltestosterone
In this study, we have developed bridge heterologous ELISA for the detection of 17α- Methyltestosterone by incorporating aromatic spacers between 17α-Methyltestosterone-3-Carboxymethyloxime and Horseradish peroxidase label through N-hydroxysuccinimide mediated carbodiimide reaction method. The immunogen 17α-Methyltestosterone-3-Carboxymethyloxime-Bovine serum albumin used to generate the antibody was also prepared by the N-hydroxysuccinimide mediated carbodiimide reaction without using any spacer. We have studied the impact of bridge/aromatic spacers on functional parameters i.e. sensitivity, affinity and ED50 of the br...
Source: Journal of Immunological Methods - September 29, 2023 Category: Allergy & Immunology Authors: Divya Verma T G Shrivastav Sonu Chand Thakur Source Type: research
Diagnosis of septic shock by serum measurement of human neutrophil lipocalin by a rapid homogeneous assay
CONCLUSIONS: As an assessment tool, we found that our AlphaLISA had good consistency with an ELISA and had several other advantages, including requiring a shorter processing time and detecting a wider range of serum HNL concentrations. Monitoring serum HNL levels of patients admitted to the ICU might be useful in distinguishing sepsis patients who have septic shock from other sepsis patients, indicating its value in the prediction of sepsis patient prognosis.PMID:37774777 | DOI:10.1016/j.jim.2023.113570 (Source: Journal of Immunological Methods)
Source: Journal of Immunological Methods - September 29, 2023 Category: Allergy & Immunology Authors: Huijun Zong Xueyi Shang Xin Wang Ting Chen Ye Wang Yuhao Ren Yongqiang Jiang Yan Li Qingyu Lv Peng Liu Source Type: research
Impact of bridge heterology on functional parameters of ELISA for 17 α-methyltestosterone
In this study, we have developed bridge heterologous ELISA for the detection of 17 α Methyl testosterone by incorporating aromatic spacers between 17α Methyl testosterone-3-Carboxymethyloxime and Horseradish peroxidase label through N-hydroxysuccinimide mediated carbodiimide reaction method. The immunogen 17α Methyl testosterone-3-Carboxymethyloxime-Bovine serum albumin used to generate the antibody was also prepared by the N-hydroxysuccinimide mediated carbodiimide reaction without using any spacer. We have studied the impact of bridge/aromatic spacers on functional parameters i.e. sensitivity, affinity and ED50 of the...
Source: Journal of Immunological Methods - September 29, 2023 Category: Allergy & Immunology Authors: Divya Verma T G Shrivastav Sonu Chand Thakur Source Type: research
Diagnosis of septic shock by serum measurement of human neutrophil lipocalin by a rapid homogeneous assay
CONCLUSIONS: As an assessment tool, we found that our AlphaLISA had good consistency with an ELISA and had several other advantages, including requiring a shorter processing time and detecting a wider range of serum HNL concentrations. Monitoring serum HNL levels of patients admitted to the ICU might be useful in distinguishing sepsis patients who have septic shock from other sepsis patients, indicating its value in the prediction of sepsis patient prognosis.PMID:37774777 | DOI:10.1016/j.jim.2023.113570 (Source: Journal of Immunological Methods)
Source: Journal of Immunological Methods - September 29, 2023 Category: Allergy & Immunology Authors: Huijun Zong Xueyi Shang Xin Wang Ting Chen Ye Wang Yuhao Ren Yongqiang Jiang Yan Li Qingyu Lv Peng Liu Source Type: research
Impact of bridge heterology on functional parameters of ELISA for 17 α-methyltestosterone
In this study, we have developed bridge heterologous ELISA for the detection of 17 α Methyl testosterone by incorporating aromatic spacers between 17α Methyl testosterone-3-Carboxymethyloxime and Horseradish peroxidase label through N-hydroxysuccinimide mediated carbodiimide reaction method. The immunogen 17α Methyl testosterone-3-Carboxymethyloxime-Bovine serum albumin used to generate the antibody was also prepared by the N-hydroxysuccinimide mediated carbodiimide reaction without using any spacer. We have studied the impact of bridge/aromatic spacers on functional parameters i.e. sensitivity, affinity and ED50 of the...
Source: Journal of Immunological Methods - September 29, 2023 Category: Allergy & Immunology Authors: Divya Verma T G Shrivastav Sonu Chand Thakur Source Type: research
Diagnosis of septic shock by serum measurement of human neutrophil lipocalin by a rapid homogeneous assay
CONCLUSIONS: As an assessment tool, we found that our AlphaLISA had good consistency with an ELISA and had several other advantages, including requiring a shorter processing time and detecting a wider range of serum HNL concentrations. Monitoring serum HNL levels of patients admitted to the ICU might be useful in distinguishing sepsis patients who have septic shock from other sepsis patients, indicating its value in the prediction of sepsis patient prognosis.PMID:37774777 | DOI:10.1016/j.jim.2023.113570 (Source: Journal of Immunological Methods)
Source: Journal of Immunological Methods - September 29, 2023 Category: Allergy & Immunology Authors: Huijun Zong Xueyi Shang Xin Wang Ting Chen Ye Wang Yuhao Ren Yongqiang Jiang Yan Li Qingyu Lv Peng Liu Source Type: research
Impact of bridge heterology on functional parameters of ELISA for 17 α-methyltestosterone
In this study, we have developed bridge heterologous ELISA for the detection of 17 α Methyl testosterone by incorporating aromatic spacers between 17α Methyl testosterone-3-Carboxymethyloxime and Horseradish peroxidase label through N-hydroxysuccinimide mediated carbodiimide reaction method. The immunogen 17α Methyl testosterone-3-Carboxymethyloxime-Bovine serum albumin used to generate the antibody was also prepared by the N-hydroxysuccinimide mediated carbodiimide reaction without using any spacer. We have studied the impact of bridge/aromatic spacers on functional parameters i.e. sensitivity, affinity and ED50 of the...
Source: Journal of Immunological Methods - September 29, 2023 Category: Allergy & Immunology Authors: Divya Verma T G Shrivastav Sonu Chand Thakur Source Type: research
Diagnosis of septic shock by serum measurement of human neutrophil lipocalin by a rapid homogeneous assay
CONCLUSIONS: As an assessment tool, we found that our AlphaLISA had good consistency with an ELISA and had several other advantages, including requiring a shorter processing time and detecting a wider range of serum HNL concentrations. Monitoring serum HNL levels of patients admitted to the ICU might be useful in distinguishing sepsis patients who have septic shock from other sepsis patients, indicating its value in the prediction of sepsis patient prognosis.PMID:37774777 | DOI:10.1016/j.jim.2023.113570 (Source: Journal of Immunological Methods)
Source: Journal of Immunological Methods - September 29, 2023 Category: Allergy & Immunology Authors: Huijun Zong Xueyi Shang Xin Wang Ting Chen Ye Wang Yuhao Ren Yongqiang Jiang Yan Li Qingyu Lv Peng Liu Source Type: research
Impact of bridge heterology on functional parameters of ELISA for 17 α-methyltestosterone
In this study, we have developed bridge heterologous ELISA for the detection of 17 α Methyl testosterone by incorporating aromatic spacers between 17α Methyl testosterone-3-Carboxymethyloxime and Horseradish peroxidase label through N-hydroxysuccinimide mediated carbodiimide reaction method. The immunogen 17α Methyl testosterone-3-Carboxymethyloxime-Bovine serum albumin used to generate the antibody was also prepared by the N-hydroxysuccinimide mediated carbodiimide reaction without using any spacer. We have studied the impact of bridge/aromatic spacers on functional parameters i.e. sensitivity, affinity and ED50 of the...
Source: Journal of Immunological Methods - September 29, 2023 Category: Allergy & Immunology Authors: Divya Verma T G Shrivastav Sonu Chand Thakur Source Type: research
Diagnosis of septic shock by serum measurement of human neutrophil lipocalin by a rapid homogeneous assay
CONCLUSIONS: As an assessment tool, we found that our AlphaLISA had good consistency with an ELISA and had several other advantages, including requiring a shorter processing time and detecting a wider range of serum HNL concentrations. Monitoring serum HNL levels of patients admitted to the ICU might be useful in distinguishing sepsis patients who have septic shock from other sepsis patients, indicating its value in the prediction of sepsis patient prognosis.PMID:37774777 | DOI:10.1016/j.jim.2023.113570 (Source: Journal of Immunological Methods)
Source: Journal of Immunological Methods - September 29, 2023 Category: Allergy & Immunology Authors: Huijun Zong Xueyi Shang Xin Wang Ting Chen Ye Wang Yuhao Ren Yongqiang Jiang Yan Li Qingyu Lv Peng Liu Source Type: research
