Comparison of capillary blood self-collection using the Tasso-SST device with venous phlebotomy for anti-SARS-CoV-2 antibody measurement
J Immunol Methods. 2023 Jul 7:113523. doi: 10.1016/j.jim.2023.113523. Online ahead of print.ABSTRACTMeasuring seroprevalence over time is a valuable epidemiological tool for improving our understanding of COVID-19 immunity. Due to the large number of collections required for population surveillance as well as concerns about potential infection risk to the collectors, self-collection approaches are being increasingly pursued. To advance this methodology, we collected paired venous and capillary blood samples by routine phlebotomy and Tasso-SST device respectively from 26 participants and measured total immunoglobulin (Ig) a...
Source: Journal of Immunological Methods - July 9, 2023 Category: Allergy & Immunology Authors: Elise R King Haley E Garrett Haley Abernathy Caitlin A Cassidy Cameron R Cabell Bonnie E Shook-Sa Jonathan J Juliano Ross M Boyce Allison E Aiello Emily J Ciccone Source Type: research
Development and characterization of a latex turbidimetric immunoassay using rabbit anti-CRP single-chain Fv antibodies
In this study, we developed and demonstrated a latex turbidimetric immunoassay (LTIA) using latex beads immobilized with rabbit monoclonal single-chain variable fragments (scFvs) selected from an scFv-displayed phage library. Sixty-five different anti-c-reactive protein (anti-CRP) scFv clones were identified after biopanning selection using antigen-coupled multi-lamellar vesicles. By ranking antigen-binding clones using the apparent dissociation rate constant (appkoff) as a sorting index, scFv clones with a dissociation constant (KD free) ranging from 4.07 × 10-9 M to 1.21 × 10-11 M were isolated. Among them, three candi...
Source: Journal of Immunological Methods - July 9, 2023 Category: Allergy & Immunology Authors: Yoichi Kumada Reina Tanibata Kimitaka Yamamoto Hiroshi Noguchi Alessandro Angelini Jun-Ichi Horiuchi Source Type: research
Comparison of capillary blood self-collection using the Tasso-SST device with venous phlebotomy for anti-SARS-CoV-2 antibody measurement
J Immunol Methods. 2023 Jul 7:113523. doi: 10.1016/j.jim.2023.113523. Online ahead of print.ABSTRACTMeasuring seroprevalence over time is a valuable epidemiological tool for improving our understanding of COVID-19 immunity. Due to the large number of collections required for population surveillance as well as concerns about potential infection risk to the collectors, self-collection approaches are being increasingly pursued. To advance this methodology, we collected paired venous and capillary blood samples by routine phlebotomy and Tasso-SST device respectively from 26 participants and measured total immunoglobulin (Ig) a...
Source: Journal of Immunological Methods - July 9, 2023 Category: Allergy & Immunology Authors: Elise R King Haley E Garrett Haley Abernathy Caitlin A Cassidy Cameron R Cabell Bonnie E Shook-Sa Jonathan J Juliano Ross M Boyce Allison E Aiello Emily J Ciccone Source Type: research
Development and characterization of a latex turbidimetric immunoassay using rabbit anti-CRP single-chain Fv antibodies
In this study, we developed and demonstrated a latex turbidimetric immunoassay (LTIA) using latex beads immobilized with rabbit monoclonal single-chain variable fragments (scFvs) selected from an scFv-displayed phage library. Sixty-five different anti-c-reactive protein (anti-CRP) scFv clones were identified after biopanning selection using antigen-coupled multi-lamellar vesicles. By ranking antigen-binding clones using the apparent dissociation rate constant (appkoff) as a sorting index, scFv clones with a dissociation constant (KD free) ranging from 4.07 × 10-9 M to 1.21 × 10-11 M were isolated. Among them, three candi...
Source: Journal of Immunological Methods - July 9, 2023 Category: Allergy & Immunology Authors: Yoichi Kumada Reina Tanibata Kimitaka Yamamoto Hiroshi Noguchi Alessandro Angelini Jun-Ichi Horiuchi Source Type: research
Comparison of capillary blood self-collection using the Tasso-SST device with venous phlebotomy for anti-SARS-CoV-2 antibody measurement
J Immunol Methods. 2023 Jul 7:113523. doi: 10.1016/j.jim.2023.113523. Online ahead of print.ABSTRACTMeasuring seroprevalence over time is a valuable epidemiological tool for improving our understanding of COVID-19 immunity. Due to the large number of collections required for population surveillance as well as concerns about potential infection risk to the collectors, self-collection approaches are being increasingly pursued. To advance this methodology, we collected paired venous and capillary blood samples by routine phlebotomy and Tasso-SST device respectively from 26 participants and measured total immunoglobulin (Ig) a...
Source: Journal of Immunological Methods - July 9, 2023 Category: Allergy & Immunology Authors: Elise R King Haley E Garrett Haley Abernathy Caitlin A Cassidy Cameron R Cabell Bonnie E Shook-Sa Jonathan J Juliano Ross M Boyce Allison E Aiello Emily J Ciccone Source Type: research
Development and characterization of a latex turbidimetric immunoassay using rabbit anti-CRP single-chain Fv antibodies
In this study, we developed and demonstrated a latex turbidimetric immunoassay (LTIA) using latex beads immobilized with rabbit monoclonal single-chain variable fragments (scFvs) selected from an scFv-displayed phage library. Sixty-five different anti-c-reactive protein (anti-CRP) scFv clones were identified after biopanning selection using antigen-coupled multi-lamellar vesicles. By ranking antigen-binding clones using the apparent dissociation rate constant (appkoff) as a sorting index, scFv clones with a dissociation constant (KD free) ranging from 4.07 × 10-9 M to 1.21 × 10-11 M were isolated. Among them, three candi...
Source: Journal of Immunological Methods - July 9, 2023 Category: Allergy & Immunology Authors: Yoichi Kumada Reina Tanibata Kimitaka Yamamoto Hiroshi Noguchi Alessandro Angelini Jun-Ichi Horiuchi Source Type: research
Dried serum spots on pre-punched filter paper discs are ready-to-use storage and shipping devices for blood-borne antigens and antibodies
J Immunol Methods. 2023 Jul 5;519:113519. doi: 10.1016/j.jim.2023.113519. Online ahead of print.ABSTRACTDried serum spots that are well prepared can be attractive alternatives to frozen serum samples for shelving specimens in a medical or research center's biobank and mailing freshly prepared serum to specialized laboratories. During the pre-analytical phase, complications can arise which are often challenging to identify or are entirely overlooked. These complications can lead to reproducibility issues, which can be avoided in serum protein analysis by implementing optimized storage and transfer procedures. With a method ...
Source: Journal of Immunological Methods - July 7, 2023 Category: Allergy & Immunology Authors: Kira Billinger Charles A Okai Manuela Russ Cornelia Koy Claudia R öwer Kwabena F M Opuni Harald Illges Ulrich Pecks Michael O Glocker Source Type: research
Dried serum spots on pre-punched filter paper discs are ready-to-use storage and shipping devices for blood-borne antigens and antibodies
J Immunol Methods. 2023 Jul 5;519:113519. doi: 10.1016/j.jim.2023.113519. Online ahead of print.ABSTRACTDried serum spots that are well prepared can be attractive alternatives to frozen serum samples for shelving specimens in a medical or research center's biobank and mailing freshly prepared serum to specialized laboratories. During the pre-analytical phase, complications can arise which are often challenging to identify or are entirely overlooked. These complications can lead to reproducibility issues, which can be avoided in serum protein analysis by implementing optimized storage and transfer procedures. With a method ...
Source: Journal of Immunological Methods - July 7, 2023 Category: Allergy & Immunology Authors: Kira Billinger Charles A Okai Manuela Russ Cornelia Koy Claudia R öwer Kwabena F M Opuni Harald Illges Ulrich Pecks Michael O Glocker Source Type: research
A novel HRM-based gap-qRT-PCR for identification and quantitation of the vaccine and field strain(s) of lumpy skin disease virus
J Immunol Methods. 2023 Jun 29;519:113521. doi: 10.1016/j.jim.2023.113521. Online ahead of print.ABSTRACTLumpy skin disease (LSD) has become the most important animal health problem in India due to high morbidity, mortality and production losses caused by it. A homologous live-attenuated LSD vaccine (Lumpi-ProVacInd) was recently developed by using a local LSD virus (LSDV) strain (LSDV/2019/India/Ranchi) in India which is likely to replace the existing practice of vaccinating cattle with goatpox vaccine. It is essential to differentiate the vaccine and field strains, if a live-attenuated vaccine has been used for control a...
Source: Journal of Immunological Methods - July 1, 2023 Category: Allergy & Immunology Authors: Ram Kumar Yogesh Chander Assim Verma Bhupendra N Tripathi Sanjay Barua Naveen Kumar Source Type: research
A novel HRM-based gap-qRT-PCR for identification and quantitation of the vaccine and field strain(s) of lumpy skin disease virus
J Immunol Methods. 2023 Jun 29;519:113521. doi: 10.1016/j.jim.2023.113521. Online ahead of print.ABSTRACTLumpy skin disease (LSD) has become the most important animal health problem in India due to high morbidity, mortality and production losses caused by it. A homologous live-attenuated LSD vaccine (Lumpi-ProVacInd) was recently developed by using a local LSD virus (LSDV) strain (LSDV/2019/India/Ranchi) in India which is likely to replace the existing practice of vaccinating cattle with goatpox vaccine. It is essential to differentiate the vaccine and field strains, if a live-attenuated vaccine has been used for control a...
Source: Journal of Immunological Methods - July 1, 2023 Category: Allergy & Immunology Authors: Ram Kumar Yogesh Chander Assim Verma Bhupendra N Tripathi Sanjay Barua Naveen Kumar Source Type: research
TA novel HRM-based gap-qRT-PCR for identification and quantitation of the vaccine and field strain(s) of lumpy skin disease virus
J Immunol Methods. 2023 Jun 29:113521. doi: 10.1016/j.jim.2023.113521. Online ahead of print.ABSTRACTLumpy skin disease (LSD) has become the most important animal health problem in India due to high morbidity, mortality and production losses. A homologous live-attenuated LSD vaccine (Lumpi-ProVacInd) was recently developed by using a local LSD virus (LSDV) strain (LSDV/2019/India/Ranchi) in India which is likely to replace the existing practice of vaccinating cattle with goatpox vaccine. It is essential to differentiate the vaccine and field strains, if a live-attenuated vaccine has been used for control and eradication of...
Source: Journal of Immunological Methods - July 1, 2023 Category: Allergy & Immunology Authors: Ram Kumar Yogesh Chander Assim Verma Bhupendra N Tripathi Sanjay Barua Naveen Kumar Source Type: research
TA novel HRM-based gap-qRT-PCR for identification and quantitation of the vaccine and field strain(s) of lumpy skin disease virus
J Immunol Methods. 2023 Jun 29:113521. doi: 10.1016/j.jim.2023.113521. Online ahead of print.ABSTRACTLumpy skin disease (LSD) has become the most important animal health problem in India due to high morbidity, mortality and production losses. A homologous live-attenuated LSD vaccine (Lumpi-ProVacInd) was recently developed by using a local LSD virus (LSDV) strain (LSDV/2019/India/Ranchi) in India which is likely to replace the existing practice of vaccinating cattle with goatpox vaccine. It is essential to differentiate the vaccine and field strains, if a live-attenuated vaccine has been used for control and eradication of...
Source: Journal of Immunological Methods - July 1, 2023 Category: Allergy & Immunology Authors: Ram Kumar Yogesh Chander Assim Verma Bhupendra N Tripathi Sanjay Barua Naveen Kumar Source Type: research
TA novel HRM-based gap-qRT-PCR for identification and quantitation of the vaccine and field strain(s) of lumpy skin disease virus
J Immunol Methods. 2023 Jun 29:113521. doi: 10.1016/j.jim.2023.113521. Online ahead of print.ABSTRACTLumpy skin disease (LSD) has become the most important animal health problem in India due to high morbidity, mortality and production losses. A homologous live-attenuated LSD vaccine (Lumpi-ProVacInd) was recently developed by using a local LSD virus (LSDV) strain (LSDV/2019/India/Ranchi) in India which is likely to replace the existing practice of vaccinating cattle with goatpox vaccine. It is essential to differentiate the vaccine and field strains, if a live-attenuated vaccine has been used for control and eradication of...
Source: Journal of Immunological Methods - July 1, 2023 Category: Allergy & Immunology Authors: Ram Kumar Yogesh Chander Assim Verma Bhupendra N Tripathi Sanjay Barua Naveen Kumar Source Type: research
TA novel HRM-based gap-qRT-PCR for identification and quantitation of the vaccine and field strain(s) of lumpy skin disease virus
J Immunol Methods. 2023 Jun 29:113521. doi: 10.1016/j.jim.2023.113521. Online ahead of print.ABSTRACTLumpy skin disease (LSD) has become the most important animal health problem in India due to high morbidity, mortality and production losses. A homologous live-attenuated LSD vaccine (Lumpi-ProVacInd) was recently developed by using a local LSD virus (LSDV) strain (LSDV/2019/India/Ranchi) in India which is likely to replace the existing practice of vaccinating cattle with goatpox vaccine. It is essential to differentiate the vaccine and field strains, if a live-attenuated vaccine has been used for control and eradication of...
Source: Journal of Immunological Methods - July 1, 2023 Category: Allergy & Immunology Authors: Ram Kumar Yogesh Chander Assim Verma Bhupendra N Tripathi Sanjay Barua Naveen Kumar Source Type: research
Effect of wash media type during PBMC isolation on downstream characterization of SARS-CoV-2-specific T cells
J Immunol Methods. 2023 Jun 28:113520. doi: 10.1016/j.jim.2023.113520. Online ahead of print.ABSTRACTProtocols for the isolation of peripheral blood mononuclear cells (PBMCs) from whole blood vary greatly between laboratories, especially in published studies of SARS-CoV-2-specific T cell responses following infection and vaccination. Research on the effects of different wash media types or centrifugation speeds and brake usage during the PBMC isolation process on downstream T cell activation and functionality is limited. Blood samples from 26 COVID-19-vaccinated participants were processed with different PBMC isolation met...
Source: Journal of Immunological Methods - June 30, 2023 Category: Allergy & Immunology Authors: Zion Congrave-Wilson Minjun Kim Aaron Sutherland Jaycee Jumarang Yesun Lee Jennifer Del Valle Wesley A Cheng Ricardo Da Silva Antunes Pia S Pannaraj Source Type: research
